Restriction map for
pFN21AE2391
(Circular) (Six-base) MAPSORT of: pFN21AE2391.seq Check: 3992 from: 1 to: 6256
pFN21AE2391 6256 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2443 2443
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2403 2525 3599 1937
Size: 466 122 1074 4594
Fragments arranged by size:
4594 1074 466 122
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2301 2631 3329 4319 5703 5971 1632
Size: 656 13 330 698 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 698 656 330 268 13
AfeI AGC'GCT
Cuts at: 3548 3548
Size: 6256
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 3084 5155 6132 1203
Size: 1881 2071 977 1327
Fragments arranged by size:
2071 1881 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 3026 1166
Size: 1860 4396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2957 3044 1184
Size: 1773 87 4396
Fragments arranged by size:
4396 1773 87
AlwNI CAG_nnn'CTG
Cuts at: 3999 5571 3999
Size: 1572 4684
ApaI G_GGCC'C
Cuts at: 3124 3124
Size: 6256
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 2285 3565 3791 3943 6052 1493
Size: 792 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 792 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 1870
Size: 73 1638 4545
Fragments arranged by size:
4545 1638 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2094 2767 3240 3577 5032
Size: 325 217 627 307 673 473 337 1455
Cuts at: 5032 618
Size: 1842
Fragments arranged by size:
1842 1455 673 627 473 337 325 307
217
BanII G_rGCy'C
Cuts at: 1391 2130 2683 3078 3124 3575 1391
Size: 739 553 395 46 451 4072
Fragments arranged by size:
4072 739 553 451 395 46
BbeI G_GCGC'C
Cuts at: 3244 3244
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2797 2840 2866 928
Size: 971 898 43 26 4318
Fragments arranged by size:
4318 971 898 43 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4447 4481 1903
Size: 34 2510 34 3678
Fragments arranged by size:
3678 2510 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3143 4148 5358 3143
Size: 1005 1210 4041
Fragments arranged by size:
4041 1210 1005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4867 136
Size: 107 122 71 631 605 3195 1525
Fragments arranged by size:
3195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2376 6251 2376
Size: 3875 2381
BlpI GC'TnA_GC
Cuts at: 3673 3673
Size: 6256
Bme1580I G_kGCm'C
Cuts at: 1790 2772 3124 3458 4317 5473 1790
Size: 982 352 334 859 1156 2573
Fragments arranged by size:
2573 1156 982 859 352 334
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2036 3987 4945
Size: 416 53 83 399 24 619 1951 958
Cuts at: 4945 442
Size: 1753
Fragments arranged by size:
1951 1753 958 619 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 3080 4916 1239
Size: 1130 711 1836 2579
Fragments arranged by size:
2579 1836 1130 711
Bpu10I CC'TnA_GC
Cuts at: 2546 2546
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 2504 3167 4381 5246 5544 2489
Size: 15 663 1214 865 298 3201
Fragments arranged by size:
3201 1214 865 663 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 3056 4919 882
Size: 617 711 846 1863 2219
Fragments arranged by size:
2219 1863 846 711 617
BsaAI yAC'GTr
Cuts at: 493 1204 4047 493
Size: 711 2843 2702
Fragments arranged by size:
2843 2702 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 3437 4063 4445
Size: 53 83 186 936 1708 196 626 382
Cuts at: 4445 5080 275
Size: 635 1451
Fragments arranged by size:
1708 1451 936 635 626 382 196 186
83 53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 3509 3539 1178
Size: 30 165 30 777 30 1299 30 3895
Fragments arranged by size:
3895 1299 777 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2093 2165 2228 2440 3148 3182 3389
Size: 904 3 72 63 212 708 34 207
Cuts at: 3389 1186
Size: 4053
Fragments arranged by size:
4053 904 708 212 207 72 63 34
3
BseYI C'CCAG_C
Cuts at: 2061 2696 3017 3094 3110 3265 3285 3501 5459
Size: 635 321 77 16 155 20 216 1958
Cuts at: 5459 5955 2061
Size: 496 2362
Fragments arranged by size:
2362 1958 635 496 321 216 155 77
20 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3065 3237 3557 1163
Size: 573 1329 172 320 3862
Fragments arranged by size:
3862 1329 573 320 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2654 3706 4467 5495 1540
Size: 275 186 653 1052 761 1028 2301
Fragments arranged by size:
2301 1052 1028 761 653 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 2247 2781 2859 2997 3078 4317 4402 5473
Size: 117 534 78 138 81 1239 85 1071
Cuts at: 5473 2130
Size: 2913
Fragments arranged by size:
2913 1239 1071 534 138 117 85 81
78
BsmI GAATG_Cn'
Cuts at: 2281 3767 3860 2281
Size: 1486 93 4677
Fragments arranged by size:
4677 1486 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2247 2683 2772 2781 2859 2997
Size: 399 340 117 436 89 9 78 138
Cuts at: 2997 3078 3124 3458 3575 4317 4402 5473 1391
Size: 81 46 334 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 340 334 138
117 117 89 85 81 78 46 9
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2443 3611 844
Size: 1599 1168 3489
Fragments arranged by size:
3489 1599 1168
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 2448 3223 4143 1394
Size: 429 260 365 775 920 3507
Fragments arranged by size:
3507 920 775 429 365 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2262 2649 2653 2815 3258 4900 5087
Size: 110 452 387 4 162 443 1642 187
Cuts at: 5087 1700
Size: 2869
Fragments arranged by size:
2869 1642 452 443 387 187 162 110
4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2962 1184
Size: 1778 4478
BstXI CCAn_nnnn'nTGG
Cuts at: 2914 2974 2914
Size: 60 6196
BstYI r'GATC_y
Cuts at: 1972 2376 3465 3586 3953 4338 4355 5807 5893
Size: 404 1089 121 367 385 17 1452 86
Cuts at: 5893 5905 6251 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1089 404 385 367 346 121
86 17 12
BstZ17I GTA'TAC
Cuts at: 2526 2526
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2586 2913 2967 513
Size: 552 939 132 450 327 54 3802
Fragments arranged by size:
3802 939 552 450 327 132 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5517
Fragments arranged by size:
5517 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3249 3772 4566 4586 3199
Size: 50 523 794 20 4869
Fragments arranged by size:
4869 794 523 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2529 2651 2655
Size: 54 1425 50 275 18 699 122 4
Cuts at: 2655 2844 2916 3234 3703 4592 6221 8
Size: 189 72 318 469 889 1629 43
Fragments arranged by size:
1629 1425 889 699 469 318 275 189
122 72 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2651 3703 1537
Size: 275 839 1052 4090
Fragments arranged by size:
4090 1052 839 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2750 2951 3059 3302 4186 1353
Size: 1397 201 108 243 884 3423
Fragments arranged by size:
3423 1397 884 243 201 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2720 4843 5217 5363 387
Size: 1064 138 1131 2123 374 146 1280
Fragments arranged by size:
2123 1280 1131 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 3076 2128
Size: 948 5308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2301 2369 2631 3080 3329 4319
Size: 393 656 13 68 262 449 249 990
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 990 787 656 597 449 393 268
262 249 68 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2555 2555
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 3120 3121 3517 3120
Size: 1 396 5859
Fragments arranged by size:
5859 396 1
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 3070 2761
Size: 309 5947
FseI GG_CCGG'CC
Cuts at: 2657 2657
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 3244 3550 5403 1678
Size: 1566 306 1853 2531
Fragments arranged by size:
2531 1853 1566 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 3200 3232 3509
Size: 32 135 32 1250 32 346 32 277
Cuts at: 3509 3541 4897 4929 4971 5003 1373
Size: 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1356 1250 346 277 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2665 2797 3343 3600 3846 669
Size: 988 281 727 132 546 257 246 3079
Fragments arranged by size:
3079 988 727 546 281 257 246 132
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3343 3846 3343
Size: 503 5753
KasI G'GCGC_C
Cuts at: 3240 3240
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 5370 5554 1305
Size: 906 3159 184 2007
Fragments arranged by size:
3159 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2846 2918 3236 10
Size: 54 1425 343 1014 72 318 3030
Fragments arranged by size:
3030 1425 1014 343 318 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 2852 2912 2972 3026 3449 4214
Size: 648 1599 87 60 60 54 423 765
Cuts at: 4214 4573 4732 518
Size: 359 159 2042
Fragments arranged by size:
2042 1599 765 648 423 359 159 87
60 60 54
MspA1I CmG'CkG
Cuts at: 1606 2061 3317 3387 3672 4349 5497 5742 1606
Size: 455 1256 70 285 677 1148 245 2120
Fragments arranged by size:
2120 1256 1148 677 455 285 245 70
NaeI GCC'GGC
Cuts at: 2655 3260 2655
Size: 605 5651
NarI GG'CG_CC
Cuts at: 3241 3241
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 2004 2913 2967 513
Size: 552 939 909 54 3802
Fragments arranged by size:
3802 939 909 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2653 3258 2653
Size: 605 5651
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 3088 3614 5159 1132
Size: 97 1859 526 1545 2229
Fragments arranged by size:
2229 1859 1545 526 97
PasI CC'CwG_GG
Cuts at: 1348 3116 3145 1348
Size: 1768 29 4459
Fragments arranged by size:
4459 1768 29
PciI A'CATG_T
Cuts at: 3084 5155 3084
Size: 2071 4185
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2235 1108
Size: 1127 5129
PfoI T'CCnGG_A
Cuts at: 3057 3057
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3517 3517
Size: 6256
PshAI GACnn'nnGTC
Cuts at: 1613 3440 1613
Size: 1827 4429
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 3120 3120
Size: 6256
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 6224
PstI C_TGCA'G
Cuts at: 830 3477 3608 830
Size: 2647 131 3478
Fragments arranged by size:
3478 2647 131
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2061 3317 3387 2061
Size: 1256 70 4930
Fragments arranged by size:
4930 1256 70
SacI G_AGCT'C
Cuts at: 2130 3078 2130
Size: 948 5308
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 3517 3517
Size: 6256
SapI GCTCTTCn'nnn_
Cuts at: 2951 3059 2951
Size: 108 6148
SbfI CC_TGCA'GG
Cuts at: 3477 3608 3477
Size: 131 6125
ScaI AGT'ACT
Cuts at: 3173 4504 6216 3173
Size: 1331 1712 3213
Fragments arranged by size:
3213 1712 1331
SexAI A'CCwGG_T
Cuts at: 2799 2799
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 3473 3604 4739 5420 5611 826
Size: 219 2428 131 1135 681 191 1471
Fragments arranged by size:
2428 1471 1135 681 219 191 131
SfoI GGC'GCC
Cuts at: 3242 3242
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 2519 3182 4360 5261 5523
Size: 197 926 561 15 663 1178 901 262
Cuts at: 5523 820
Size: 1553
Fragments arranged by size:
1553 1178 926 901 663 561 262 197
15
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6256
StyI C'CwwG_G
Cuts at: 513 1065 2004 2565 2913 2967 3159 3695 513
Size: 552 939 561 348 54 192 536 3074
Fragments arranged by size:
3074 939 561 552 536 348 192 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3031 3038 4283 4300 4453 4638 5083
Size: 1255 1451 7 1245 17 153 185 445
Cuts at: 5083 325
Size: 1498
Fragments arranged by size:
1498 1451 1255 1245 445 185 153 17
7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3171 4502
Size: 274 80 33 51 1443 444 750 1331
Cuts at: 4502 6214 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1331 750 444 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 6216 6248 2312
Size: 32 3872 32 2320
Fragments arranged by size:
3872 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2665 2683 3509 2665
Size: 18 826 5412
Fragments arranged by size:
5412 826 18
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 3053 4385 3053
Size: 1332 4924
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI Bsu36I DraIII EcoRV FspAI NruI NsiI PacI
PmlI PspXI RsrII SacII SfiI SrfI SwaI