Restriction map for
pFN21AE2390
(Circular) (Six-base) MAPSORT of: pFN21AE2390.seq Check: 7646 from: 1 to: 6208
pFN21AE2390 6208 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2089 3551 1937
Size: 152 1462 4594
Fragments arranged by size:
4594 1462 152
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3392 4271 5655 5923 1632
Size: 1760 879 1384 268 1917
Fragments arranged by size:
1917 1760 1384 879 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 2231 2504 1166
Size: 1065 273 4870
Fragments arranged by size:
4870 1065 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 2454 3951 5523 2454
Size: 1497 1572 3139
Fragments arranged by size:
3139 1572 1497
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2368 3517 3743 3895 6004 1493
Size: 875 1149 226 152 2109 1697
Fragments arranged by size:
2109 1697 1149 875 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 3098 3533 1870
Size: 73 491 664 435 4545
Fragments arranged by size:
4545 664 491 435 73
AvrII C'CTAG_G
Cuts at: 2675 2675
Size: 6208
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 3529 4984 618
Size: 325 217 627 260 1482 1455 1842
Fragments arranged by size:
1842 1482 1455 627 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2020 2841 3123 3527 1391
Size: 629 821 282 404 4072
Fragments arranged by size:
4072 821 629 404 282
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 928
Size: 971 895 4342
Fragments arranged by size:
4342 971 895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BfrBI ATG'CAT
Cuts at: 2772 2772
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2650 4819 136
Size: 107 122 71 631 605 978 2169 1525
Fragments arranged by size:
2169 1525 978 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3048 6203 3048
Size: 3155 3053
BlpI GC'TnA_GC
Cuts at: 2267 3625 2267
Size: 1358 4850
Bme1580I G_kGCm'C
Cuts at: 1790 4269 5425 1790
Size: 2479 1156 2573
Fragments arranged by size:
2573 2479 1156
BmgBI CAC'GTC
Cuts at: 1318 2341 2377 1318
Size: 1023 36 5149
Fragments arranged by size:
5149 1023 36
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2649 3123 3477 4868 1239
Size: 1028 382 474 354 1391 2579
Fragments arranged by size:
2579 1391 1028 474 382 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4333 5198 5496 4333
Size: 865 298 5045
Fragments arranged by size:
5045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2145 2180 2897 3099 4871 882
Size: 617 646 35 717 202 1772 2219
Fragments arranged by size:
2219 1772 717 646 617 202 35
BsaAI yAC'GTr
Cuts at: 493 1204 3477 3999 493
Size: 711 2273 522 2702
Fragments arranged by size:
2702 2273 711 522
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 2064 3471 4015
Size: 53 83 186 936 515 16 1407 544
Cuts at: 4015 4397 5032 275
Size: 382 635 1451
Fragments arranged by size:
1451 1407 936 635 544 515 382 186
83 53 16
BsaWI w'CCGG_w
Cuts at: 3180 3295 4641 5039 5313 5460 5871 3180
Size: 115 1346 398 274 147 411 3517
Fragments arranged by size:
3517 1346 411 398 274 147 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2142 2172 1178
Size: 30 165 30 739 30 5214
Fragments arranged by size:
5214 739 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3307 1186
Size: 2121 4087
BseYI C'CCAG_C
Cuts at: 2032 2041 2271 3150 5411 5907 2032
Size: 9 230 879 2261 496 2333
Fragments arranged by size:
2333 2261 879 496 230 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2984 1163
Size: 573 1248 4387
Fragments arranged by size:
4387 1248 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3658 4419 5447 1540
Size: 275 186 1657 761 1028 2301
Fragments arranged by size:
2301 1657 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2228 2289 4269 4354 5425 2228
Size: 61 1980 85 1071 3011
Fragments arranged by size:
3011 1980 1071 85 61
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 3319 3319
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2228 2289 2841 3123 3527 4269
Size: 399 230 208 61 552 282 404 742
Cuts at: 4269 4354 5425 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 404 399 282 230
208 85 61
BspEI T'CCGG_A
Cuts at: 3180 3295 5871 3180
Size: 115 2576 3517
Fragments arranged by size:
3517 2576 115
BspHI T'CATG_A
Cuts at: 2596 4097 5827 2596
Size: 1501 1730 2977
Fragments arranged by size:
2977 1730 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2817 3006 3084 3563 844
Size: 1973 189 78 479 3489
Fragments arranged by size:
3489 1973 479 189 78
BsrBI CCG'CTC
Cuts at: 1394 1823 2053 3009 4095 1394
Size: 429 230 956 1086 3507
Fragments arranged by size:
3507 1086 956 429 230
BsrDI GCAATG_nn'
Cuts at: 65 1248 2760 4703 4877 65
Size: 1183 1512 1943 174 1396
Fragments arranged by size:
1943 1512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2454 794
Size: 1660 4548
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 2467 1184
Size: 1283 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 3403 3403
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 3048 3538 3905 4290 4307 5759 5845 5857
Size: 1076 490 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 490 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2068 2949 3396 513
Size: 552 1003 881 447 3325
Fragments arranged by size:
3325 1003 881 552 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3316 487
Size: 683 56 2090 3379
Fragments arranged by size:
3379 2090 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 2298 3264 1554
Size: 744 966 4498
Fragments arranged by size:
4498 966 744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2393 5215 809
Size: 507 1077 2822 1802
Fragments arranged by size:
2822 1802 1077 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2620 2790
Size: 54 1425 50 275 18 302 488 170
Cuts at: 2790 3393 3655 4544 6173 8
Size: 603 262 889 1629 43
Fragments arranged by size:
1629 1425 889 603 488 302 275 262
170 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 2747 2790 3209 3419 4138 1353
Size: 858 536 43 419 210 719 3423
Fragments arranged by size:
3423 858 719 536 419 210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 2333 2717 4795 5169 5315
Size: 1064 138 507 237 384 2078 374 146
Cuts at: 5315 387
Size: 1280
Fragments arranged by size:
2078 1280 1064 507 384 374 237 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2649 3123 3392 3477 4271 4868
Size: 393 635 382 474 269 85 794 597
Cuts at: 4868 5655 5923 1239
Size: 787 268 1524
Fragments arranged by size:
1524 794 787 635 597 474 393 382
269 268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2679 2679
Size: 6208
EcoO109I rG'GnC_Cy
Cuts at: 2275 2561 3450 2275
Size: 286 889 5033
Fragments arranged by size:
5033 889 286
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2758 2254
Size: 504 5704
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2042 2051 2701 5355 1678
Size: 364 9 650 2654 2531
Fragments arranged by size:
2654 2531 650 364 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 4849 4881 4923
Size: 32 135 32 570 32 2675 32 42
Cuts at: 4923 4955 1373
Size: 32 2626
Fragments arranged by size:
2675 2626 570 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2090 2755 3552 3798 669
Size: 988 281 152 665 797 246 3079
Fragments arranged by size:
3079 988 797 665 281 246 152
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 5322 5506 1305
Size: 1128 2889 184 2007
Fragments arranged by size:
2889 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2622 2792 3395 10
Size: 54 1425 343 790 170 603 2823
Fragments arranged by size:
2823 1425 790 603 343 170 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2504 2595 3401 4166 4525 4684
Size: 648 1065 273 91 806 765 359 159
Cuts at: 4684 518
Size: 2042
Fragments arranged by size:
2042 1065 806 765 648 359 273 159
91
MspA1I CmG'CkG
Cuts at: 1606 2070 2454 3070 3343 3624 4301 5449 5694
Size: 464 384 616 273 281 677 1148 245
Cuts at: 5694 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 616 464 384 281 273
245
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 2949 3396 513
Size: 552 1884 447 3325
Fragments arranged by size:
3325 1884 552 447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NsiI A_TGCA'T
Cuts at: 2774 2774
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2012 1348
Size: 664 5544
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3146 1108
Size: 2038 4170
PfoI T'CCnGG_A
Cuts at: 2094 3139 3465 2094
Size: 1045 326 4837
Fragments arranged by size:
4837 1045 326
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2561 3450 2275
Size: 286 889 5033
Fragments arranged by size:
5033 889 286
PshAI GACnn'nnGTC
Cuts at: 1613 3455 1613
Size: 1842 4366
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2459 2550 3348 3560 830
Size: 1629 91 798 212 3478
Fragments arranged by size:
3478 1629 798 212 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2454 3343 2454
Size: 889 5319
SacII CC_GC'GG
Cuts at: 2071 2071
Size: 6208
SalI G'TCGA_C
Cuts at: 1936 2088 3550 1936
Size: 152 1462 4594
Fragments arranged by size:
4594 1462 152
SanDI GG'GwC_CC
Cuts at: 2275 3450 2275
Size: 1175 5033
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2748 2748
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 2260 2455 2518 2546 3344 3556 4691
Size: 219 1215 195 63 28 798 212 1135
Cuts at: 4691 5372 5563 826
Size: 681 191 1471
Fragments arranged by size:
1471 1215 1135 798 681 219 212 195
191 63 28
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2436 3535 1872
Size: 564 1099 4545
Fragments arranged by size:
4545 1099 564
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4312 5213 5475 820
Size: 197 926 2369 901 262 1553
Fragments arranged by size:
2369 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 3173 4132 5
Size: 47 3121 959 2081
Fragments arranged by size:
3121 2081 959 47
StuI AGG'CCT
Cuts at: 1517 2556 1517
Size: 1039 5169
StyI C'CwwG_G
Cuts at: 513 1065 2675 2949 3396 3647 513
Size: 552 1610 274 447 251 3074
Fragments arranged by size:
3074 1610 552 447 274 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 4235 4252 4405 4590 5035 325
Size: 1255 1455 1200 17 153 185 445 1498
Fragments arranged by size:
1498 1455 1255 1200 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 4454 6166
Size: 274 80 33 51 1443 258 2219 1712
Cuts at: 6166 96
Size: 138
Fragments arranged by size:
2219 1712 1443 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 6168 6200 2312
Size: 32 3824 32 2320
Fragments arranged by size:
3824 2320 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2156 1316
Size: 840 5368
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3147 3147
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2434 3533 1870
Size: 564 1099 4545
Fragments arranged by size:
4545 1099 564
XmnI GAAnn'nnTTC
Cuts at: 2105 4337 2105
Size: 2232 3976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SapI SfiI SrfI SwaI