Restriction map for
pFN21AE2385
(Circular) (Six-base) MAPSORT of: pFN21AE2385.seq Check: 9450 from: 1 to: 6583
pFN21AE2385 6583 bp
With 182 enzymes: SgfI *
November 30, 2011 11:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4393 278
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3926 1937
Size: 1989 4594
Acc65I G'GTAC_C
Cuts at: 3904 3904
Size: 6583
AclI AA'CG_TT
Cuts at: 1563 4710 5083 1563
Size: 3147 373 3063
Fragments arranged by size:
3147 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2528 4646 6030 6298 1632
Size: 893 3 2118 1384 268 1917
Fragments arranged by size:
2118 1917 1384 893 268 3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6386
AflIII A'CryG_T
Cuts at: 1203 3545 3856 5482 6459 1203
Size: 2342 311 1626 977 1327
Fragments arranged by size:
2342 1626 1327 977 311
AgeI A'CCGG_T
Cuts at: 5414 5414
Size: 6583
AhdI GACnn_n'nnGTC
Cuts at: 5312 5312
Size: 6583
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6583
AlwNI CAG_nnn'CTG
Cuts at: 3582 4326 5898 3582
Size: 744 1572 4267
Fragments arranged by size:
4267 1572 744
ApaLI G'TGCA_C
Cuts at: 4640 5796 4640
Size: 1156 5427
ApoI r'AATT_y
Cuts at: 1493 2224 2335 3008 3445 3567 3892 4118 4270
Size: 731 111 673 437 122 325 226 152
Cuts at: 4270 6379 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 731 673 437 325 226 152
122 111
AseI AT'TA_AT
Cuts at: 160 1032 5137 160
Size: 872 4105 1606
Fragments arranged by size:
4105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
AvaI C'yCGr_G
Cuts at: 1870 1943 3908 1870
Size: 73 1965 4545
Fragments arranged by size:
4545 1965 73
BamHI G'GATC_C
Cuts at: 3670 3913 4280 3670
Size: 243 367 5973
Fragments arranged by size:
5973 367 243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3904 5359 618
Size: 325 217 627 2117 1455 1842
Fragments arranged by size:
2117 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3902 1391
Size: 2511 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2258 2790 3690 928
Size: 971 359 532 900 3821
Fragments arranged by size:
3821 971 900 532 359
BbvCI CC'TCA_GC
Cuts at: 2344 2344
Size: 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3797 3831 4774 4808 1903
Size: 34 1860 34 943 34 3678
Fragments arranged by size:
3678 1860 943 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3409 4475 5685 3409
Size: 1066 1210 4307
Fragments arranged by size:
4307 1210 1066
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3122 5194 136
Size: 107 122 71 631 605 1450 2072 1525
Fragments arranged by size:
2072 1525 1450 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6578 6578
Size: 6583
BlpI GC'TnA_GC
Cuts at: 4000 4000
Size: 6583
Bme1580I G_kGCm'C
Cuts at: 1790 2204 4644 5800 1790
Size: 414 2440 1156 2573
Fragments arranged by size:
2573 2440 1156 414
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6583
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3283 3371 3645
Size: 416 53 83 399 24 1866 88 274
Cuts at: 3645 4314 5272 442
Size: 669 958 1753
Fragments arranged by size:
1866 1753 958 669 416 399 274 88
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2613 3435 2613
Size: 822 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2026 3852 5243 1239
Size: 787 1826 1391 2579
Fragments arranged by size:
2579 1826 1391 787
Bpu10I CC'TnA_GC
Cuts at: 2145 2344 2145
Size: 199 6384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4708 5573 5871 4708
Size: 865 298 5420
Fragments arranged by size:
5420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2104 5246 882
Size: 617 605 3142 2219
Fragments arranged by size:
3142 2219 617 605
BsaAI yAC'GTr
Cuts at: 493 1204 4374 493
Size: 711 3170 2702
Fragments arranged by size:
3170 2702 711
BsaBI GATnn'nnATC
Cuts at: 3558 6577 3558
Size: 3019 3564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4390 4772 5407 275
Size: 53 83 186 936 2857 382 635 1451
Fragments arranged by size:
2857 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5016 5414 5688 5835 6246 5016
Size: 398 274 147 411 5353
Fragments arranged by size:
5353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3051 3081 1178
Size: 30 165 30 1648 30 4680
Fragments arranged by size:
4680 1648 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3596 1186
Size: 2410 4173
BseYI C'CCAG_C
Cuts at: 3039 5786 6282 3039
Size: 2747 496 3340
Fragments arranged by size:
3340 2747 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 1163
Size: 573 1062 4948
Fragments arranged by size:
4948 1062 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4033 4794 5822 1540
Size: 275 186 2032 761 1028 2301
Fragments arranged by size:
2301 2032 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4644 4729 5800 4644
Size: 85 1071 5427
Fragments arranged by size:
5427 1071 85
BsmI GAATG_Cn'
Cuts at: 3757 4094 4187 3757
Size: 337 93 6153
Fragments arranged by size:
6153 337 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 3902 4644 4729 5800 1391
Size: 399 414 1698 742 85 1071 2174
Fragments arranged by size:
2174 1698 1071 742 414 399 85
BspEI T'CCGG_A
Cuts at: 6246 6246
Size: 6583
BspHI T'CATG_A
Cuts at: 4472 6202 4472
Size: 1730 4853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3938 844
Size: 3094 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3604 4470 1394
Size: 429 1781 866 3507
Fragments arranged by size:
3507 1781 866 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3495 5078 5252 65
Size: 1183 2247 1583 174 1396
Fragments arranged by size:
2247 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5227 5414 1700
Size: 110 3417 187 2869
Fragments arranged by size:
3417 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6583
BssSI C'ACGA_G
Cuts at: 4643 5655 4643
Size: 1012 5571
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3248 794
Size: 2454 4129
BstBI TT'CG_AA
Cuts at: 5387 5387
Size: 6583
BstEII G'GTnAC_C
Cuts at: 1184 3538 1184
Size: 2354 4229
BstYI r'GATC_y
Cuts at: 1972 3670 3913 4280 4665 4682 6134 6220 6232
Size: 1698 243 367 385 17 1452 86 12
Cuts at: 6232 6578 1972
Size: 346 1977
Fragments arranged by size:
1977 1698 1452 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 2411 3655 487
Size: 683 56 851 334 1244 3415
Fragments arranged by size:
3415 1244 851 683 334 56
BtsI GCAGTG_nn'
Cuts at: 3399 4099 4893 4913 3399
Size: 700 794 20 5069
Fragments arranged by size:
5069 794 700 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6583
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3647 3682 529
Size: 35 3083 35 3430
Fragments arranged by size:
3430 3083 35 35
DraI TTT'AAA
Cuts at: 2803 2898 3887 2803
Size: 95 989 5499
Fragments arranged by size:
5499 989 95
DraIII CAC_nnn'GTG
Cuts at: 3379 3379
Size: 6583
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5590 809
Size: 507 4274 1802
Fragments arranged by size:
4274 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4030 4919 6548
Size: 54 1425 50 275 18 2200 889 1629
Cuts at: 6548 8
Size: 43
Fragments arranged by size:
2200 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4030 1537
Size: 275 2218 4090
Fragments arranged by size:
4090 2218 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 2547 2582 3186 4513 1353
Size: 1139 55 35 604 1327 3423
Fragments arranged by size:
3423 1327 1139 604 55 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 3493 5170 5544 5690 387
Size: 1064 138 462 1442 1677 374 146 1280
Fragments arranged by size:
1677 1442 1280 1064 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2525 2528 3852 4646 5243 6030
Size: 393 394 499 3 1324 794 597 787
Cuts at: 6030 6298 1239
Size: 268 1524
Fragments arranged by size:
1524 1324 794 787 597 499 394 393
268 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2491 2491
Size: 6583
EcoRI G'AATT_C
Cuts at: 2224 3445 3892 4270 2224
Size: 1221 447 378 4537
Fragments arranged by size:
4537 1221 447 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3041 3480 3041
Size: 439 6144
FspI TGC'GCA
Cuts at: 5089 5089
Size: 6583
HaeII r_GCGC'y
Cuts at: 1678 5730 1678
Size: 4052 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 5224 5256 5298
Size: 32 135 32 700 32 2920 32 42
Cuts at: 5298 5330 1373
Size: 32 2626
Fragments arranged by size:
2920 2626 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3927 4173 669
Size: 988 281 1989 246 3079
Fragments arranged by size:
3079 1989 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6583
HpaI GTT'AAC
Cuts at: 4173 4173
Size: 6583
KpnI G_GTAC'C
Cuts at: 3908 3908
Size: 6583
MfeI C'AATT_G
Cuts at: 4182 4182
Size: 6583
MluI A'CGCG_T
Cuts at: 6459 6459
Size: 6583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2729 3001 5697 5881 1305
Size: 1424 272 2696 184 2007
Fragments arranged by size:
2696 2007 1424 272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4761
Fragments arranged by size:
4761 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3276 3426 4541 4900 5059 518
Size: 648 2110 150 1115 359 159 2042
Fragments arranged by size:
2110 2042 1115 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2348 3582 3846 3999 4676 5824 6069 1606
Size: 742 1234 264 153 677 1148 245 2120
Fragments arranged by size:
2120 1234 1148 742 677 264 245 153
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6031
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6583
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6583
NotI GC'GGCC_GC
Cuts at: 4030 4030
Size: 6583
NspI r_CATG'y
Cuts at: 1132 1229 3549 3860 3941 5486 1132
Size: 97 2320 311 81 1545 2229
Fragments arranged by size:
2320 2229 1545 311 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6583
PciI A'CATG_T
Cuts at: 3545 3856 5482 3545
Size: 311 1626 4646
Fragments arranged by size:
4646 1626 311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6583
PfoI T'CCnGG_A
Cuts at: 3449 3449
Size: 6583
PmeI GTTT'AAAC
Cuts at: 3887 3887
Size: 6583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4652 4684 6165 6197 4652
Size: 32 1481 32 5038
Fragments arranged by size:
5038 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6583
PsiI TTA'TAA
Cuts at: 4153 4153
Size: 6583
PstI C_TGCA'G
Cuts at: 830 3935 830
Size: 3105 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6583
PvuII CAG'CTG
Cuts at: 2348 3582 2348
Size: 1234 5349
SalI G'TCGA_C
Cuts at: 1936 3925 1936
Size: 1989 4594
SbfI CC_TGCA'GG
Cuts at: 3935 3935
Size: 6583
ScaI AGT'ACT
Cuts at: 4831 6543 4831
Size: 1712 4871
SexAI A'CCwGG_T
Cuts at: 3650 3650
Size: 6583
SfcI C'TryA_G
Cuts at: 826 1045 2124 3931 5066 5747 5938 826
Size: 219 1079 1807 1135 681 191 1471
Fragments arranged by size:
1807 1471 1135 1079 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6583
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6583
SmaI CCC'GGG
Cuts at: 1872 3910 1872
Size: 2038 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4687 5588 5850 820
Size: 197 926 2744 901 262 1553
Fragments arranged by size:
2744 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4374 493
Size: 3881 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6583
SphI G_CATG'C
Cuts at: 1132 3941 1132
Size: 2809 3774
SspI AAT'ATT
Cuts at: 5 52 2952 4507 5
Size: 47 2900 1555 2081
Fragments arranged by size:
2900 2081 1555 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6583
StyI C'CwwG_G
Cuts at: 513 1065 2313 4022 513
Size: 552 1248 1709 3074
Fragments arranged by size:
3074 1709 1248 552
SwaI ATTT'AAAT
Cuts at: 2898 2898
Size: 6583
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3390 3825 4610 4627 4780 4965 5410
Size: 1255 1810 435 785 17 153 185 445
Cuts at: 5410 325
Size: 1498
Fragments arranged by size:
1810 1498 1255 785 445 435 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4829 6541 96
Size: 274 80 33 51 1443 2852 1712 138
Fragments arranged by size:
2852 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3738 3770 6543 6575 3738
Size: 32 2773 32 3746
Fragments arranged by size:
3746 2773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3689 1316
Size: 2373 4210
XbaI T'CTAG_A
Cuts at: 3919 3919
Size: 6583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 3343 2461
Size: 882 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6583
XmaI C'CCGG_G
Cuts at: 1870 3908 1870
Size: 2038 4545
XmnI GAAnn'nnTTC
Cuts at: 4712 4712
Size: 6583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4391 276
Size: 53 83 186 936 2857 2468
Fragments arranged by size:
2857 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI