Restriction map for
pFN21AE2384
(Circular) (Six-base) MAPSORT of: pFN21AE2384.seq Check: 2569 from: 1 to: 5653
pFN21AE2384 5653 bp
With 182 enzymes: SgfI *
November 11, 2011 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2074 2191 2074
Size: 117 5536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 3463 278
Size: 53 83 186 936 582 1345 2468
Fragments arranged by size:
2468 1345 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2060 2121 2974 2060
Size: 61 853 4739
Fragments arranged by size:
4739 853 61
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 3716 5100 5368 1632
Size: 692 1392 1384 268 1917
Fragments arranged by size:
1917 1392 1384 692 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2424 4552 5529 1203
Size: 1221 2128 977 1327
Fragments arranged by size:
2128 1327 1221 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2661 2749 2889 3396 4968 2661
Size: 88 140 507 1572 3346
Fragments arranged by size:
3346 1572 507 140 88
ApaLI G'TGCA_C
Cuts at: 2245 3710 4866 2245
Size: 1465 1156 3032
Fragments arranged by size:
3032 1465 1156
ApoI r'AATT_y
Cuts at: 1493 2366 2962 3188 3340 5449 1493
Size: 873 596 226 152 2109 1697
Fragments arranged by size:
2109 1697 873 596 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 2978 1870
Size: 73 729 306 4545
Fragments arranged by size:
4545 729 306 73
BamHI G'GATC_C
Cuts at: 2695 2983 3350 2695
Size: 288 367 4998
Fragments arranged by size:
4998 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2060 2121 2974 4429
Size: 325 217 627 246 27 61 853 1455
Cuts at: 4429 618
Size: 1842
Fragments arranged by size:
1842 1455 853 627 325 246 217 61
27
BanII G_rGCy'C
Cuts at: 1391 2223 2972 1391
Size: 832 749 4072
Fragments arranged by size:
4072 832 749
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 5653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2294 928
Size: 971 395 4287
Fragments arranged by size:
4287 971 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2643 4264 136
Size: 107 122 71 631 605 971 1621 1525
Fragments arranged by size:
1621 1525 971 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2249 3714 4870 1790
Size: 353 106 1465 1156 2573
Fragments arranged by size:
2573 1465 1156 353 106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2397 3384 4342
Size: 416 53 83 399 24 980 987 958
Cuts at: 4342 442
Size: 1753
Fragments arranged by size:
1753 987 980 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2483 2711 2911 4313 1239
Size: 822 422 228 200 1402 2579
Fragments arranged by size:
2579 1402 822 422 228 200
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2250 2427 3444 493
Size: 711 1046 177 1017 2702
Fragments arranged by size:
2702 1046 1017 711 177
BsaBI GATnn'nnATC
Cuts at: 2045 5647 2045
Size: 3602 2051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2115 3460 3842
Size: 53 83 186 936 501 81 1345 382
Cuts at: 3842 4477 275
Size: 635 1451
Fragments arranged by size:
1451 1345 936 635 501 382 186 83
81 53
BsaWI w'CCGG_w
Cuts at: 2754 4086 4484 4758 4905 5316 2754
Size: 1332 398 274 147 411 3091
Fragments arranged by size:
3091 1332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2884 2914 1178
Size: 30 165 30 1481 30 3917
Fragments arranged by size:
3917 1481 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 4608
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2249 3714 3799 4870 2223
Size: 26 1465 85 1071 3006
Fragments arranged by size:
3006 1465 1071 85 26
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2223 2249 2972 3714 3799 4870
Size: 399 353 80 26 723 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 723 399 353 85 80
26
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2191 3008 844
Size: 1230 117 817 3489
Fragments arranged by size:
3489 1230 817 117
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2398 2811 4148 4322 65
Size: 1183 1150 413 1337 174 1396
Fragments arranged by size:
1396 1337 1183 1150 413 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2623 1184
Size: 1439 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 2419 2419
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2695 2983 3350 3735 3752 5204 5290 5302
Size: 723 288 367 385 17 1452 86 12
Cuts at: 5302 5648 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 723 385 367 346 288 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2436 513
Size: 552 939 432 3730
Fragments arranged by size:
3730 939 552 432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2175 2339
Size: 54 1425 50 275 18 276 69 164
Cuts at: 2339 2433 3100 3989 5618 8
Size: 94 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 276 275 164 94
69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 2436 2940 3583 1353
Size: 875 208 504 643 3423
Fragments arranged by size:
3423 875 643 504 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 2221
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2324 2483 2711 2911 3716 4313
Size: 393 429 263 159 228 200 805 597
Cuts at: 4313 5100 5368 1239
Size: 787 268 1524
Fragments arranged by size:
1524 805 787 597 429 393 268 263
228 200 159
EcoO109I rG'GnC_Cy
Cuts at: 2263 2658 2677 2746 2263
Size: 395 19 69 5170
Fragments arranged by size:
5170 395 69 19
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2037 4800 1678
Size: 359 2763 2531
Fragments arranged by size:
2763 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373
Size: 32 135 32 2722 32 42 32 2626
Fragments arranged by size:
2722 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2551 2997 3243 669
Size: 988 281 613 446 246 3079
Fragments arranged by size:
3079 988 613 446 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 2551 3243 2551
Size: 692 4961
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 5653
KpnI G_GTAC'C
Cuts at: 2064 2125 2978 2064
Size: 61 853 4739
Fragments arranged by size:
4739 853 61
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2508 4767 4951 1305
Size: 1203 2259 184 2007
Fragments arranged by size:
2259 2007 1203 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2177 10
Size: 54 1425 343 276 69 3486
Fragments arranged by size:
3486 1425 343 276 69 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3611 3970 4129 518
Size: 648 1455 990 359 159 2042
Fragments arranged by size:
2042 1455 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3069 3746 4894 5139 1606
Size: 1463 677 1148 245 2120
Fragments arranged by size:
2120 1463 1148 677 245
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 2004 2436 513
Size: 552 939 432 3730
Fragments arranged by size:
3730 939 552 432
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2661 1348
Size: 1313 4340
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PfoI T'CCnGG_A
Cuts at: 2688 2688
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PmlI CAC'GTG
Cuts at: 2427 2427
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2677 2746 2677
Size: 69 5584
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2548 3005 830
Size: 1718 457 3478
Fragments arranged by size:
3478 1718 457
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
SacI G_AGCT'C
Cuts at: 2223 2223
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2228 2436 2228
Size: 208 5445
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 2600 3901 5613 2600
Size: 1301 1712 2640
Fragments arranged by size:
2640 1712 1301
SfcI C'TryA_G
Cuts at: 826 1045 2544 2632 2736 3001 4136 4817 5008
Size: 219 1499 88 104 265 1135 681 191
Cuts at: 5008 826
Size: 1471
Fragments arranged by size:
1499 1471 1135 681 265 219 191 104
88
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 5653
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 3092 513
Size: 552 939 432 656 3074
Fragments arranged by size:
3074 939 656 552 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2583 3680 3697 3850 4035 4480 325
Size: 1255 1003 1097 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1097 1003 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 3899 5611
Size: 274 80 33 51 1443 621 1301 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1443 1301 621 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2884 2916 5613 5645 2884
Size: 32 2697 32 2892
Fragments arranged by size:
2892 2697 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2443 2617 2053
Size: 390 174 5089
Fragments arranged by size:
5089 390 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 3461 276
Size: 53 83 186 936 582 1345 2468
Fragments arranged by size:
2468 1345 936 582 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SrfI SwaI