Restriction map for
pFN21AE2383
(Circular) (Six-base) MAPSORT of: pFN21AE2383.seq Check: 9396 from: 1 to: 6175
pFN21AE2383 6175 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2130 3518 1937
Size: 193 1388 4594
Fragments arranged by size:
4594 1388 193
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 4302 4675 1563
Size: 2739 373 3063
Fragments arranged by size:
3063 2739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3464 4238 5622 5890 1632
Size: 1832 774 1384 268 1917
Fragments arranged by size:
1917 1832 1384 774 268
AfeI AGC'GCT
Cuts at: 3184 3184
Size: 6175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5978
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3201 1184
Size: 2017 4158
AlwNI CAG_nnn'CTG
Cuts at: 2683 2779 3166 3377 3918 5490 2683
Size: 96 387 211 541 1572 3368
Fragments arranged by size:
3368 1572 541 387 211 96
ApaLI G'TGCA_C
Cuts at: 4232 5388 4232
Size: 1156 5019
ApoI r'AATT_y
Cuts at: 1493 3117 3248 3484 3710 3862 5971 1493
Size: 1624 131 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1624 236 226 152 131
AseI AT'TA_AT
Cuts at: 160 1032 2994 4729 160
Size: 872 1962 1735 1606
Fragments arranged by size:
1962 1735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 3500 1870
Size: 73 1002 555 4545
Fragments arranged by size:
4545 1002 555 73
BamHI G'GATC_C
Cuts at: 2418 3505 3872 2418
Size: 1087 367 4721
Fragments arranged by size:
4721 1087 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2589 3053 3083 3496 4951
Size: 325 217 627 802 464 30 413 1455
Cuts at: 4951 618
Size: 1842
Fragments arranged by size:
1842 1455 802 627 464 413 325 217
30
BanII G_rGCy'C
Cuts at: 1391 2875 3494 1391
Size: 1484 619 4072
Fragments arranged by size:
4072 1484 619
BbeI G_GCGC'C
Cuts at: 2593 3057 2593
Size: 464 5711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 3304 928
Size: 971 429 976 3799
Fragments arranged by size:
3799 976 971 429
BbvCI CC'TCA_GC
Cuts at: 2818 2818
Size: 6175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4366 4400 1903
Size: 34 2429 34 3678
Fragments arranged by size:
3678 2429 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 5277 4067
Size: 1210 4965
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BfrBI ATG'CAT
Cuts at: 2269 2509 2269
Size: 240 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6170 6170
Size: 6175
BlpI GC'TnA_GC
Cuts at: 2610 3592 2610
Size: 982 5193
Bme1580I G_kGCm'C
Cuts at: 1790 4236 5392 1790
Size: 2446 1156 2573
Fragments arranged by size:
2573 2446 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3906 4864 442
Size: 416 53 83 399 24 2489 958 1753
Fragments arranged by size:
2489 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 6175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2695 2988 4835 1239
Size: 1340 116 293 1847 2579
Fragments arranged by size:
2579 1847 1340 293 116
Bpu10I CC'TnA_GC
Cuts at: 2818 2818
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2243 4300 5165 5463 2243
Size: 2057 865 298 2955
Fragments arranged by size:
2955 2057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3249 4838 882
Size: 617 1750 1589 2219
Fragments arranged by size:
2219 1750 1589 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 6169 6169
Size: 6175
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2590 3054 3093 3982
Size: 53 83 186 936 1057 464 39 889
Cuts at: 3982 4364 4999 275
Size: 382 635 1451
Fragments arranged by size:
1451 1057 936 889 635 464 382 186
83 53 39
BsaWI w'CCGG_w
Cuts at: 2154 4608 5006 5280 5427 5838 2154
Size: 2454 398 274 147 411 2491
Fragments arranged by size:
2491 2454 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2961 2991 1178
Size: 30 165 30 1558 30 4362
Fragments arranged by size:
4362 1558 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2698 1186
Size: 1512 4663
BseYI C'CCAG_C
Cuts at: 2659 5378 5874 2659
Size: 2719 496 2960
Fragments arranged by size:
2960 2719 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2202 2225 2940 3363 3458 1163
Size: 573 466 23 715 423 95 3880
Fragments arranged by size:
3880 715 573 466 423 95 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4236 4321 5392 4236
Size: 85 1071 5019
Fragments arranged by size:
5019 1071 85
BsmI GAATG_Cn'
Cuts at: 2239 2417 2497 2736 2764 3686 3779 2239
Size: 178 80 239 28 922 93 4635
Fragments arranged by size:
4635 922 239 178 93 80 28
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2875 3494 4236 4321 5392 1391
Size: 399 1085 619 742 85 1071 2174
Fragments arranged by size:
2174 1085 1071 742 619 399 85
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2299 3530 844
Size: 1455 1231 3489
Fragments arranged by size:
3489 1455 1231
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 4670 4844 65
Size: 1183 1239 2183 174 1396
Fragments arranged by size:
2183 1396 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3153 96
Size: 3057 3118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 2854 2878 4235 5247 2854
Size: 24 1357 1012 3782
Fragments arranged by size:
3782 1357 1012 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2492 3386 794
Size: 1698 894 3583
Fragments arranged by size:
3583 1698 894
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2719 2317
Size: 402 5773
BstYI r'GATC_y
Cuts at: 1972 2070 2418 3505 3872 4257 4274 5726 5812
Size: 98 348 1087 367 385 17 1452 86
Cuts at: 5812 5824 6170 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1087 385 367 348 346 98
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2523 2886 3087 513
Size: 552 1458 363 201 3601
Fragments arranged by size:
3601 1458 552 363 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 2237 2631 2910 3691 4485 4505 2237
Size: 394 279 781 794 20 3907
Fragments arranged by size:
3907 794 781 394 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 3479 3479
Size: 6175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3371 5182 809
Size: 507 2055 1811 1802
Fragments arranged by size:
2055 1811 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2398 3622 4511
Size: 54 1425 50 275 18 568 1224 889
Cuts at: 4511 6140 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1224 889 568 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2542 4105 1353
Size: 1189 1563 3423
Fragments arranged by size:
3423 1563 1189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2679 4762 5136 5282 387
Size: 1064 138 1090 2083 374 146 1280
Fragments arranged by size:
2083 1280 1090 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 2695 2988 3464 4238 4835 5622
Size: 393 947 116 293 476 774 597 787
Cuts at: 5622 5890 1239
Size: 268 1524
Fragments arranged by size:
1524 947 787 774 597 476 393 293
268 116
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 3101 3242 2696
Size: 405 141 5629
Fragments arranged by size:
5629 405 141
EcoO109I rG'GnC_Cy
Cuts at: 2429 2429
Size: 6175
EcoRI G'AATT_C
Cuts at: 3117 3484 3862 3117
Size: 367 378 5430
Fragments arranged by size:
5430 378 367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2237
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 2593 2690 2865 3057 3186 5322 1678
Size: 915 97 175 192 129 2136 2531
Fragments arranged by size:
2531 2136 915 192 175 129 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 2374 2406 4816
Size: 32 135 32 685 32 85 32 2410
Cuts at: 4816 4848 4890 4922 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2410 685 135 85 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6175
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KasI G'GCGC_C
Cuts at: 2589 3053 2589
Size: 464 5711
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 2067 2334 2959 5289 5473 1305
Size: 739 23 267 625 2330 184 2007
Fragments arranged by size:
2330 2007 739 625 267 184 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2400 10
Size: 54 1425 343 568 3785
Fragments arranged by size:
3785 1425 568 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2282 2342 2378 4133 4492 4651
Size: 648 888 228 60 36 1755 359 159
Cuts at: 4651 518
Size: 2042
Fragments arranged by size:
2042 1755 888 648 359 228 159 60
36
MspA1I CmG'CkG
Cuts at: 1606 2733 2804 3442 3591 4268 5416 5661 1606
Size: 1127 71 638 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 1127 677 638 245 149 71
NarI GG'CG_CC
Cuts at: 2590 3054 2590
Size: 464 5711
NcoI C'CATG_G
Cuts at: 513 1065 2523 3087 513
Size: 552 1458 564 3601
Fragments arranged by size:
3601 1458 564 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6175
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NsiI A_TGCA'T
Cuts at: 2271 2511 2271
Size: 240 5935
NspI r_CATG'y
Cuts at: 1132 1229 3533 5078 1132
Size: 97 2304 1545 2229
Fragments arranged by size:
2304 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2866 1348
Size: 1518 4657
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6175
PfoI T'CCnGG_A
Cuts at: 2148 2148
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 2429
Size: 6175
PshAI GACnn'nnGTC
Cuts at: 1613 2157 1613
Size: 544 5631
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2296 2697 2803 3174 3190 3273 3527 830
Size: 1466 401 106 371 16 83 254 3478
Fragments arranged by size:
3478 1466 401 371 254 106 83 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
PvuII CAG'CTG
Cuts at: 2804 3442 2804
Size: 638 5537
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SapI GCTCTTCn'nnn_
Cuts at: 2542 2542
Size: 6175
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2292 2693 2799 3170 3186 3269 3523
Size: 219 1247 401 106 371 16 83 254
Cuts at: 3523 4658 5339 5530 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1247 1135 681 401 371 254 219
191 106 83 16
SfoI GGC'GCC
Cuts at: 2591 3055 2591
Size: 464 5711
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 3502 1872
Size: 1630 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 4279 5180 5442 820
Size: 197 926 279 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 279 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 2248 2992 4099 5
Size: 47 2196 744 1107 2081
Fragments arranged by size:
2196 2081 1107 744 47
StuI AGG'CCT
Cuts at: 1517 3107 1517
Size: 1590 4585
StyI C'CwwG_G
Cuts at: 513 1065 2523 3087 3614 513
Size: 552 1458 564 527 3074
Fragments arranged by size:
3074 1458 564 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2711 3116 4202 4219 4372 4557 5002
Size: 1255 1131 405 1086 17 153 185 445
Cuts at: 5002 325
Size: 1498
Fragments arranged by size:
1498 1255 1131 1086 445 405 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3153 4421 6133
Size: 274 80 33 51 1443 1176 1268 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
1712 1443 1268 1176 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3401 3401
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 3500 1870
Size: 1630 4545
XmnI GAAnn'nnTTC
Cuts at: 4304 4304
Size: 6175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SrfI SwaI