Restriction map for
pFN21AE2377
(Circular) (Six-base) MAPSORT of: pFN21AE2377.seq Check: 7298 from: 1 to: 8875
pFN21AE2377 8875 bp
With 182 enzymes: SgfI *
October 27, 2011 14:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6685 278
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2590 6218 1937
Size: 653 3628 4594
Fragments arranged by size:
4594 3628 653
Acc65I G'GTAC_C
Cuts at: 6196 6196
Size: 8875
AclI AA'CG_TT
Cuts at: 1563 7002 7375 1563
Size: 5439 373 3063
Fragments arranged by size:
5439 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2241 2568 2760 3123 3252 3957 4539 6145
Size: 609 327 192 363 129 705 582 1606
Cuts at: 6145 6938 8322 8590 1632
Size: 793 1384 268 1917
Fragments arranged by size:
1917 1606 1384 793 705 609 582 363
327 268 192 129
AflII C'TTAA_G
Cuts at: 820 1017 4981 820
Size: 197 3964 4714
Fragments arranged by size:
4714 3964 197
AflIII A'CryG_T
Cuts at: 1203 2216 4526 4593 7774 8751 1203
Size: 1013 2310 67 3181 977 1327
Fragments arranged by size:
3181 2310 1327 1013 977 67
AgeI A'CCGG_T
Cuts at: 7706 7706
Size: 8875
AhdI GACnn_n'nnGTC
Cuts at: 7604 7604
Size: 8875
AleI CACnn'nnGTG
Cuts at: 1166 3075 4115 1166
Size: 1909 1040 5926
Fragments arranged by size:
5926 1909 1040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8875
AlwNI CAG_nnn'CTG
Cuts at: 4105 6618 8190 4105
Size: 2513 1572 4790
Fragments arranged by size:
4790 2513 1572
ApaLI G'TGCA_C
Cuts at: 6932 8088 6932
Size: 1156 7719
ApoI r'AATT_y
Cuts at: 1493 2251 3175 3365 3372 3782 4187 4796 5313
Size: 758 924 190 7 410 405 609 517
Cuts at: 5313 5392 5499 6064 6184 6410 6562 8671 1493
Size: 79 107 565 120 226 152 2109 1697
Fragments arranged by size:
2109 1697 924 758 609 565 517 410
405 226 190 152 120 107 79 7
AseI AT'TA_AT
Cuts at: 160 1032 4020 7429 160
Size: 872 2988 3409 1606
Fragments arranged by size:
3409 2988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
AvaI C'yCGr_G
Cuts at: 1870 1943 5895 6200 1870
Size: 73 3952 305 4545
Fragments arranged by size:
4545 3952 305 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5114 5147 5114
Size: 33 8842
BamHI G'GATC_C
Cuts at: 6205 6572 6205
Size: 367 8508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3162 4432 5528 6196 7651
Size: 325 217 627 1375 1270 1096 668 1455
Cuts at: 7651 618
Size: 1842
Fragments arranged by size:
1842 1455 1375 1270 1096 668 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2066 6194 1391
Size: 675 4128 4072
Fragments arranged by size:
4128 4072 675
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 2619 2757 5295 5658 5769 5780
Size: 971 480 240 138 2538 363 111 11
Cuts at: 5780 6023 928
Size: 243 3780
Fragments arranged by size:
3780 2538 971 480 363 243 240 138
111 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7066 7100 1903
Size: 34 5129 34 3678
Fragments arranged by size:
5129 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 6767 7977 3896
Size: 2871 1210 4794
Fragments arranged by size:
4794 2871 1210
BclI T'GATC_A
Cuts at: 1548 3556 1548
Size: 2008 6867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 7486 136
Size: 107 122 71 631 605 463 5351 1525
Fragments arranged by size:
5351 1525 631 605 463 122 107 71
BglII A'GATC_T
Cuts at: 2228 3975 8870 2228
Size: 1747 4895 2233
Fragments arranged by size:
4895 2233 1747
BlpI GC'TnA_GC
Cuts at: 4830 6292 4830
Size: 1462 7413
Bme1580I G_kGCm'C
Cuts at: 1790 4534 6936 8092 1790
Size: 2744 2402 1156 2573
Fragments arranged by size:
2744 2573 2402 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4877 6606 7564
Size: 416 53 83 399 24 3460 1729 958
Cuts at: 7564 442
Size: 1753
Fragments arranged by size:
3460 1753 1729 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3674 3887 1056
Size: 2618 213 6044
Fragments arranged by size:
6044 2618 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3803
Size: 8875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 3600 4653 5232 7535 1239
Size: 878 1483 1053 579 2303 2579
Fragments arranged by size:
2579 2303 1483 1053 878 579
Bpu10I CC'TnA_GC
Cuts at: 4032 4899 4032
Size: 867 8008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5604 7000 7865 8163 5604
Size: 1396 865 298 6316
Fragments arranged by size:
6316 1396 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5639 7538 882
Size: 617 4140 1899 2219
Fragments arranged by size:
4140 2219 1899 617
BsaAI yAC'GTr
Cuts at: 493 1204 2219 4594 6666 493
Size: 711 1015 2375 2072 2702
Fragments arranged by size:
2702 2375 2072 1015 711
BsaBI GATnn'nnATC
Cuts at: 3246 3849 5163 5205 8869 3246
Size: 603 1314 42 3664 3252
Fragments arranged by size:
3664 3252 1314 603 42
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6682 7064 7699 275
Size: 53 83 186 936 5149 382 635 1451
Fragments arranged by size:
5149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2211 4774 5648 7308 7706 7980 8127 8538 2211
Size: 2563 874 1660 398 274 147 411 2548
Fragments arranged by size:
2563 2548 1660 874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 7996
Fragments arranged by size:
7996 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3088 5338 1186
Size: 1902 2250 4723
Fragments arranged by size:
4723 2250 1902
BseYI C'CCAG_C
Cuts at: 2977 8078 8574 2977
Size: 5101 496 3278
Fragments arranged by size:
5101 3278 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 3475 1163
Size: 573 282 1457 6563
Fragments arranged by size:
6563 1457 573 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2110 2130 6325 7086 8114 1540
Size: 275 186 109 20 4195 761 1028 2301
Fragments arranged by size:
4195 2301 1028 761 275 186 109 20
BsiHKAI G_wGCw'C
Cuts at: 2103 2367 3442 6936 7021 8092 2103
Size: 264 1075 3494 85 1071 2886
Fragments arranged by size:
3494 2886 1075 1071 264 85
BsmI GAATG_Cn'
Cuts at: 5584 6386 6479 5584
Size: 802 93 7980
Fragments arranged by size:
7980 802 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2103 2367 3442 4534 6194 6936
Size: 399 276 37 264 1075 1092 1660 742
Cuts at: 6936 7021 8092 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1660 1092 1075 1071 742 399 276
264 85 37
BspEI T'CCGG_A
Cuts at: 8538 8538
Size: 8875
BspHI T'CATG_A
Cuts at: 3486 5992 6764 8494 3486
Size: 2506 772 1730 3867
Fragments arranged by size:
3867 2506 1730 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6230 844
Size: 5386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 6762 1394
Size: 429 301 4638 3507
Fragments arranged by size:
4638 3507 429 301
BsrDI GCAATG_nn'
Cuts at: 65 1248 7370 7544 65
Size: 1183 6122 174 1396
Fragments arranged by size:
6122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2129 7519 7706 1700
Size: 110 319 5390 187 2869
Fragments arranged by size:
5390 2869 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 2658 5786 96
Size: 2562 3128 3185
Fragments arranged by size:
3185 3128 2562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8875
BssSI C'ACGA_G
Cuts at: 6935 7947 6935
Size: 1012 7863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3236 794
Size: 2442 6433
BstBI TT'CG_AA
Cuts at: 7679 7679
Size: 8875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8875
BstXI CCAn_nnnn'nTGG
Cuts at: 3862 3862
Size: 8875
BstYI r'GATC_y
Cuts at: 1972 2228 3745 3975 4006 5206 6205 6572 6957
Size: 256 1517 230 31 1200 999 367 385
Cuts at: 6957 6974 8426 8512 8524 8870 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1517 1452 1200 999 385 367 346
256 230 86 31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2058 2151 513
Size: 552 939 42 12 93 7237
Fragments arranged by size:
7237 939 552 93 42 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2190 6086 487
Size: 683 56 964 3896 3276
Fragments arranged by size:
3896 3276 964 683 56
BtsI GCAGTG_nn'
Cuts at: 2541 2731 6391 7185 7205 2541
Size: 190 3660 794 20 4211
Fragments arranged by size:
4211 3660 794 190 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5386 5421 529
Size: 35 4822 35 3983
Fragments arranged by size:
4822 3983 35 35
DraI TTT'AAA
Cuts at: 3370 6179 3370
Size: 2809 6066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3027 7882 809
Size: 507 1711 4855 1802
Fragments arranged by size:
4855 1802 1711 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2107 2127
Size: 54 1425 50 275 18 225 52 20
Cuts at: 2127 2331 6322 7211 8840 8
Size: 204 3991 889 1629 43
Fragments arranged by size:
3991 1629 1425 889 275 225 204 54
52 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2107 2127 6322 1537
Size: 275 295 20 4195 4090
Fragments arranged by size:
4195 4090 295 275 20
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2736 2862 3873 4018 4713 5486 6805
Size: 1002 381 126 1011 145 695 773 1319
Cuts at: 6805 1353
Size: 3423
Fragments arranged by size:
3423 1319 1011 1002 773 695 381 145
126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3142 7462 7836 7982 387
Size: 1064 138 1553 4320 374 146 1280
Fragments arranged by size:
4320 1553 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2241 2568 2760 3123 3252 3600
Size: 393 485 124 327 192 363 129 348
Cuts at: 3600 3957 4539 4653 5232 6145 6938 7535 8322
Size: 357 582 114 579 913 793 597 787
Cuts at: 8322 8590 1239
Size: 268 1524
Fragments arranged by size:
1524 913 793 787 597 582 579 485
393 363 357 348 327 268 192 129
124 114
EcoRI G'AATT_C
Cuts at: 2251 3782 4187 6184 6562 2251
Size: 1531 405 1997 378 4564
Fragments arranged by size:
4564 1997 1531 405 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2573 3048 5297 2573
Size: 475 2249 6151
Fragments arranged by size:
6151 2249 475
FspI TGC'GCA
Cuts at: 7381 7381
Size: 8875
HaeII r_GCGC'y
Cuts at: 1678 8022 1678
Size: 6344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 2473 2505 3908
Size: 32 135 32 596 32 273 32 1403
Cuts at: 3908 3940 4256 4288 4892 4924 5084 5116 5402
Size: 32 316 32 604 32 160 32 286
Cuts at: 5402 5434 6107 6139 7516 7548 7590 7622 1373
Size: 32 673 32 1377 32 42 32 2626
Fragments arranged by size:
2626 1403 1377 673 604 596 316 286
273 160 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4144 5044 6219 6465 669
Size: 988 281 2206 900 1175 246 3079
Fragments arranged by size:
3079 2206 1175 988 900 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8875
HpaI GTT'AAC
Cuts at: 6465 6465
Size: 8875
KpnI G_GTAC'C
Cuts at: 6200 6200
Size: 8875
MfeI C'AATT_G
Cuts at: 6474 6474
Size: 8875
MluI A'CGCG_T
Cuts at: 8751 8751
Size: 8875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3880 3984 4591 6039 7989 8173 1305
Size: 2575 104 607 1448 1950 184 2007
Fragments arranged by size:
2575 2007 1950 1448 607 184 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 6552
Fragments arranged by size:
6552 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 2766 3075 4115 6833 7192 7351
Size: 648 1273 327 309 1040 2718 359 159
Cuts at: 7351 518
Size: 2042
Fragments arranged by size:
2718 2042 1273 1040 648 359 327 309
159
MspA1I CmG'CkG
Cuts at: 1606 2048 2153 4439 6291 6968 8116 8361 1606
Size: 442 105 2286 1852 677 1148 245 2120
Fragments arranged by size:
2286 2120 1852 1148 677 442 245 105
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 8875
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7384
Fragments arranged by size:
7384 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8875
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 8875
NheI G'CTAG_C
Cuts at: 1052 3670 3883 1052
Size: 2618 213 6044
Fragments arranged by size:
6044 2618 213
NotI GC'GGCC_GC
Cuts at: 6322 6322
Size: 8875
NspI r_CATG'y
Cuts at: 1132 1229 3065 4184 4530 5787 6233 7778 1132
Size: 97 1836 1119 346 1257 446 1545 2229
Fragments arranged by size:
2229 1836 1545 1257 1119 446 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8875
PciI A'CATG_T
Cuts at: 4526 7774 4526
Size: 3248 5627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3846 4412 1108
Size: 2738 566 5571
Fragments arranged by size:
5571 2738 566
PfoI T'CCnGG_A
Cuts at: 5209 5209
Size: 8875
PmeI GTTT'AAAC
Cuts at: 6179 6179
Size: 8875
PmlI CAC'GTG
Cuts at: 2219 4594 2219
Size: 2375 6500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6944 6976 8457 8489 6944
Size: 32 1481 32 7330
Fragments arranged by size:
7330 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8875
PsiI TTA'TAA
Cuts at: 2534 3207 6445 2534
Size: 673 3238 4964
Fragments arranged by size:
4964 3238 673
PspXI vC'TCGA_Gb
Cuts at: 5895 5895
Size: 8875
PstI C_TGCA'G
Cuts at: 830 4002 4167 6227 830
Size: 3172 165 2060 3478
Fragments arranged by size:
3478 3172 2060 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8875
PvuII CAG'CTG
Cuts at: 4439 4439
Size: 8875
SacII CC_GC'GG
Cuts at: 2049 2154 2049
Size: 105 8770
SalI G'TCGA_C
Cuts at: 1936 6217 1936
Size: 4281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2355 3873 2355
Size: 1518 7357
SbfI CC_TGCA'GG
Cuts at: 6227 6227
Size: 8875
ScaI AGT'ACT
Cuts at: 4507 5458 7123 8835 4507
Size: 951 1665 1712 4547
Fragments arranged by size:
4547 1712 1665 951
SfcI C'TryA_G
Cuts at: 826 1045 3741 3998 4163 5173 5778 6223 7358
Size: 219 2696 257 165 1010 605 445 1135
Cuts at: 7358 8039 8230 826
Size: 681 191 1471
Fragments arranged by size:
2696 1471 1135 1010 681 605 445 257
219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8875
SmaI CCC'GGG
Cuts at: 1872 6202 1872
Size: 4330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4981 5619 5895 6979 7880 8142
Size: 197 926 3038 638 276 1084 901 262
Cuts at: 8142 820
Size: 1553
Fragments arranged by size:
3038 1553 1084 926 901 638 276 262
197
SnaBI TAC'GTA
Cuts at: 493 6666 493
Size: 6173 2702
SpeI A'CTAG_T
Cuts at: 152 3986 152
Size: 3834 5041
SphI G_CATG'C
Cuts at: 1132 3065 6233 1132
Size: 1933 3168 3774
Fragments arranged by size:
3774 3168 1933
SspI AAT'ATT
Cuts at: 5 52 4389 4654 6799 5
Size: 47 4337 265 2145 2081
Fragments arranged by size:
4337 2145 2081 265 47
StuI AGG'CCT
Cuts at: 1517 3563 3763 1517
Size: 2046 200 6629
Fragments arranged by size:
6629 2046 200
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 6314 513
Size: 552 939 63 4247 3074
Fragments arranged by size:
4247 3074 939 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 4630 6902 6919 7072 7257 7702
Size: 1255 805 2245 2272 17 153 185 445
Cuts at: 7702 325
Size: 1498
Fragments arranged by size:
2272 2245 1498 1255 805 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2426 2554 2658
Size: 274 80 33 51 1443 449 128 104
Cuts at: 2658 4505 5456 5786 7121 8833 96
Size: 1847 951 330 1335 1712 138
Fragments arranged by size:
1847 1712 1443 1335 951 449 330 274
138 128 104 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 8835 8867 2027
Size: 32 6776 32 2035
Fragments arranged by size:
6776 2035 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8875
XbaI T'CTAG_A
Cuts at: 5324 6211 5324
Size: 887 7988
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2065 2065
Size: 8875
XhoI C'TCGA_G
Cuts at: 1943 5895 1943
Size: 3952 4923
XmaI C'CCGG_G
Cuts at: 1870 6200 1870
Size: 4330 4545
XmnI GAAnn'nnTTC
Cuts at: 5313 5728 7004 5313
Size: 415 1276 7184
Fragments arranged by size:
7184 1276 415
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6683 276
Size: 53 83 186 936 5149 2468
Fragments arranged by size:
5149 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI