Restriction map for
pFN21AE2374
(Circular) (Six-base) MAPSORT of: pFN21AE2374.seq Check: 3846 from: 1 to: 5557
pFN21AE2374 5557 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3367 278
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2784 2900 1937
Size: 847 116 4594
Fragments arranged by size:
4594 847 116
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3620 5004 5272 1632
Size: 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 2343 4456 5433 1203
Size: 1140 2113 977 1327
Fragments arranged by size:
2113 1327 1140 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 2083 2725 4286 2083
Size: 642 1561 3354
Fragments arranged by size:
3354 1561 642
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2479 2520 3300 4872 2479
Size: 41 780 1572 3164
Fragments arranged by size:
3164 1572 780 41
ApaI G_GGCC'C
Cuts at: 2060 2060
Size: 5557
ApaLI G'TGCA_C
Cuts at: 2372 2696 3614 4770 2372
Size: 324 918 1156 3159
Fragments arranged by size:
3159 1156 918 324
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2507 2619 2730 2882 1870
Size: 73 116 448 112 111 152 4545
Fragments arranged by size:
4545 448 152 116 112 111 73
BamHI G'GATC_C
Cuts at: 2161 2887 3254 2161
Size: 726 367 4464
Fragments arranged by size:
4464 726 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 4333 618
Size: 325 217 627 1091 1455 1842
Fragments arranged by size:
1842 1455 1091 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 2620 2688 2876 1391
Size: 669 560 68 188 4072
Fragments arranged by size:
4072 669 560 188 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BbvCI CC'TCA_GC
Cuts at: 2564 2564
Size: 5557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4168 136
Size: 107 122 71 631 605 2496 1525
Fragments arranged by size:
2496 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2841 5552 2841
Size: 2711 2846
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2376 2700 3618 4774 1790
Size: 270 316 324 918 1156 2573
Fragments arranged by size:
2573 1156 918 324 316 270
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5557
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3288 4246
Size: 416 53 83 399 24 1206 665 958
Cuts at: 4246 442
Size: 1753
Fragments arranged by size:
1753 1206 958 665 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2471 2776 4217 1239
Size: 1232 305 1441 2579
Fragments arranged by size:
2579 1441 1232 305
Bpu10I CC'TnA_GC
Cuts at: 2564 2564
Size: 5557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 3682 4547 4845 2564
Size: 1118 865 298 3276
Fragments arranged by size:
3276 1118 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2346 2692 3348 493
Size: 711 1142 346 656 2702
Fragments arranged by size:
2702 1142 711 656 346
BsaBI GATnn'nnATC
Cuts at: 2828 5551 2828
Size: 2723 2834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3364 3746 4381 275
Size: 53 83 186 936 1831 382 635 1451
Fragments arranged by size:
1831 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5332
Fragments arranged by size:
5332 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 2750 1186
Size: 1309 255 3993
Fragments arranged by size:
3993 1309 255
BseYI C'CCAG_C
Cuts at: 2050 2072 2401 2769 4760 5256 2050
Size: 22 329 368 1991 496 2351
Fragments arranged by size:
2351 1991 496 368 329 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2041 1163
Size: 573 305 4679
Fragments arranged by size:
4679 573 305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 3768 4796 1540
Size: 275 186 1006 761 1028 2301
Fragments arranged by size:
2301 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2376 2688 2700 3618 3703 4774 2376
Size: 312 12 918 85 1071 3159
Fragments arranged by size:
3159 1071 918 312 85 12
BsmI GAATG_Cn'
Cuts at: 3068 3161 3068
Size: 93 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2376 2620 2688 2700 2876 3618
Size: 399 270 316 244 68 12 176 742
Cuts at: 3618 3703 4774 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 316 270 244 176
85 68 12
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 2826 3446 5176 2826
Size: 620 1730 3207
Fragments arranged by size:
3207 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2730 2845 2912 844
Size: 1886 115 67 3489
Fragments arranged by size:
3489 1886 115 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 2467 3444 1394
Size: 429 316 328 977 3507
Fragments arranged by size:
3507 977 429 328 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 4052 4226 65
Size: 1183 2804 174 1396
Fragments arranged by size:
2804 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2634 4201 4388 1700
Size: 110 824 1567 187 2869
Fragments arranged by size:
2869 1567 824 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 2327 3617 4629 2327
Size: 1290 1012 3255
Fragments arranged by size:
3255 1290 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5557
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2161 2223 2841 2887 3254 3639 3656 5108
Size: 189 62 618 46 367 385 17 1452
Cuts at: 5108 5194 5206 5552 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 618 385 367 346 189 86
62 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 4058
Fragments arranged by size:
4058 939 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 2376 2661 3073 3867 3887 2376
Size: 285 412 794 20 4046
Fragments arranged by size:
4046 794 412 285 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5522
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DraIII CAC_nnn'GTG
Cuts at: 2158 2158
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3004 3893 5522
Size: 54 1425 50 275 18 1174 889 1629
Cuts at: 5522 8
Size: 43
Fragments arranged by size:
1629 1425 1174 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3004 1537
Size: 275 1192 4090
Fragments arranged by size:
4090 1192 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2756 3487 1353
Size: 1403 731 3423
Fragments arranged by size:
3423 1403 731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4144 4518 4664 387
Size: 1064 138 2555 374 146 1280
Fragments arranged by size:
2555 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2686
Size: 5557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2776 3620 4217 5004 5272 1239
Size: 393 839 305 844 597 787 268 1524
Fragments arranged by size:
1524 844 839 787 597 393 305 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2306 2276
Size: 30 5527
EcoO109I rG'GnC_Cy
Cuts at: 2056 2063 2546 2056
Size: 7 483 5067
Fragments arranged by size:
5067 483 7
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 4704 1678
Size: 3026 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2165 2197 2711
Size: 32 135 32 497 32 64 32 514
Cuts at: 2711 2743 2833 2865 4198 4230 4272 4304 1373
Size: 32 90 32 1333 32 42 32 2626
Fragments arranged by size:
2626 1333 514 497 135 90 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2901 3147 669
Size: 988 281 963 246 3079
Fragments arranged by size:
3079 988 963 281 246
HindIII A'AGCT_T
Cuts at: 748 2294 748
Size: 1546 4011
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3735
Fragments arranged by size:
3735 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2304 3515 3874 4033 518
Size: 648 864 274 1211 359 159 2042
Fragments arranged by size:
2042 1211 864 648 359 274 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2093 2130 2500 2973 3650 4798 5043
Size: 408 79 37 370 473 677 1148 245
Cuts at: 5043 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 473 408 370 245 79
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 3004 3004
Size: 5557
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5557
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5557
PfoI T'CCnGG_A
Cuts at: 2486 2486
Size: 5557
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PmlI CAC'GTG
Cuts at: 2346 2346
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 2546
Size: 5557
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PspOMI G'GGCC_C
Cuts at: 2056 2056
Size: 5557
PspXI vC'TCGA_Gb
Cuts at: 2507 2507
Size: 5557
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2438 2470 2438
Size: 32 5525
PstI C_TGCA'G
Cuts at: 830 2092 2727 2856 2909 830
Size: 1262 635 129 53 3478
Fragments arranged by size:
3478 1262 635 129 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
PvuII CAG'CTG
Cuts at: 2093 2130 2500 2093
Size: 37 370 5150
Fragments arranged by size:
5150 370 37
SacI G_AGCT'C
Cuts at: 2688 2688
Size: 5557
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SanDI GG'GwC_CC
Cuts at: 2546 2546
Size: 5557
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SfcI C'TryA_G
Cuts at: 826 1045 2088 2723 2852 2905 4040 4721 4912
Size: 219 1043 635 129 53 1135 681 191
Cuts at: 4912 826
Size: 1471
Fragments arranged by size:
1471 1135 1043 681 635 219 191 129
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2061 2884 1872
Size: 189 823 4545
Fragments arranged by size:
4545 823 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 2579 3661 4562 4824 820
Size: 197 926 564 72 1082 901 262 1553
Fragments arranged by size:
1553 1082 926 901 564 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2996 513
Size: 552 939 108 884 3074
Fragments arranged by size:
3074 939 884 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3584 3601 3754 3939 4384 325
Size: 1255 2004 17 153 185 445 1498
Fragments arranged by size:
2004 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2299 2299
Size: 5557
XhoI C'TCGA_G
Cuts at: 1943 2507 1943
Size: 564 4993
XmaI C'CCGG_G
Cuts at: 1870 2059 2882 1870
Size: 189 823 4545
Fragments arranged by size:
4545 823 189
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3365 276
Size: 53 83 186 936 1831 2468
Fragments arranged by size:
2468 1831 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI RsrII SapI SexAI SfiI SfoI SrfI SwaI