Restriction map for
pFN21AE2371
(Circular) (Six-base) MAPSORT of: pFN21AE2371.seq Check: 4351 from: 1 to: 7075
pFN21AE2371 7075 bp
With 182 enzymes: SgfI *
October 11, 2011 14:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2727 4885 278
Size: 53 83 186 936 1191 2158 2468
Fragments arranged by size:
2468 2158 1191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3228 4418 1937
Size: 1291 1190 4594
Fragments arranged by size:
4594 1291 1190
Acc65I G'GTAC_C
Cuts at: 4396 4396
Size: 7075
AclI AA'CG_TT
Cuts at: 1563 5202 5575 1563
Size: 3639 373 3063
Fragments arranged by size:
3639 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 2607 3095 3788 3862 5138 6522 6790
Size: 749 226 488 693 74 1276 1384 268
Cuts at: 6790 1632
Size: 1917
Fragments arranged by size:
1917 1384 1276 749 693 488 268 226
74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6878
AflIII A'CryG_T
Cuts at: 1203 3321 5974 6951 1203
Size: 2118 2653 977 1327
Fragments arranged by size:
2653 2118 1327 977
AgeI A'CCGG_T
Cuts at: 5906 5906
Size: 7075
AhdI GACnn_n'nnGTC
Cuts at: 5804 5804
Size: 7075
AleI CACnn'nnGTG
Cuts at: 1166 3185 1166
Size: 2019 5056
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 7043
AlwNI CAG_nnn'CTG
Cuts at: 2845 4818 6390 2845
Size: 1973 1572 3530
Fragments arranged by size:
3530 1973 1572
ApaLI G'TGCA_C
Cuts at: 2124 2316 5132 6288 2124
Size: 192 2816 1156 2911
Fragments arranged by size:
2911 2816 1156 192
ApoI r'AATT_y
Cuts at: 1493 4355 4384 4610 4762 6871 1493
Size: 2862 29 226 152 2109 1697
Fragments arranged by size:
2862 2109 1697 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 5629 160
Size: 872 4597 1606
Fragments arranged by size:
4597 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 3393 4261 4400 1870
Size: 73 885 565 868 139 4545
Fragments arranged by size:
4545 885 868 565 139 73
AvrII C'CTAG_G
Cuts at: 3457 3457
Size: 7075
BamHI G'GATC_C
Cuts at: 2568 4405 4772 2568
Size: 1837 367 4871
Fragments arranged by size:
4871 1837 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2448 2717 4080 4396
Size: 325 217 627 242 419 269 1363 316
Cuts at: 4396 5851 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1363 627 419 325 316 269
242 217
BanII G_rGCy'C
Cuts at: 1391 4394 1391
Size: 3003 4072
BbeI G_GCGC'C
Cuts at: 2452 2721 4084 2452
Size: 269 1363 5443
Fragments arranged by size:
5443 1363 269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 2516 3607 4238 4263 928
Size: 971 286 331 1091 631 25 3740
Fragments arranged by size:
3740 1091 971 631 331 286 25
BbvCI CC'TCA_GC
Cuts at: 4136 4136
Size: 7075
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5266 5300 1903
Size: 34 3329 34 3678
Fragments arranged by size:
3678 3329 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3963 4967 6177 3963
Size: 1004 1210 4861
Fragments arranged by size:
4861 1210 1004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7075
BfrBI ATG'CAT
Cuts at: 4337 4337
Size: 7075
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 5686 136
Size: 107 122 71 631 605 1072 2942 1525
Fragments arranged by size:
2942 1525 1072 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7070 7070
Size: 7075
BlpI GC'TnA_GC
Cuts at: 4492 4492
Size: 7075
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2320 5136 6292 1790
Size: 338 192 2816 1156 2573
Fragments arranged by size:
2816 2573 1156 338 192
BmgBI CAC'GTC
Cuts at: 1318 4240 1318
Size: 2922 4153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 2257 2307
Size: 416 53 83 399 24 638 202 50
Cuts at: 2307 3004 3151 4806 5764 442
Size: 697 147 1655 958 1753
Fragments arranged by size:
1753 1655 958 697 638 416 399 202
147 83 53 50 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7075
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 7075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 3023 3290 5735 1239
Size: 920 864 267 2445 2579
Fragments arranged by size:
2579 2445 920 864 267
Bpu10I CC'TnA_GC
Cuts at: 4136 4136
Size: 7075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5200 6065 6363 5200
Size: 865 298 5912
Fragments arranged by size:
5912 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2282 2999 3523 5738 882
Size: 617 783 717 524 2215 2219
Fragments arranged by size:
2219 2215 783 717 617 524
BsaAI yAC'GTr
Cuts at: 493 1204 4866 493
Size: 711 3662 2702
Fragments arranged by size:
3662 2702 711
BsaBI GATnn'nnATC
Cuts at: 7069 7069
Size: 7075
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2449 2718 2724 2795
Size: 53 83 186 936 916 269 6 71
Cuts at: 2795 4081 4882 5264 5899 275
Size: 1286 801 382 635 1451
Fragments arranged by size:
1451 1286 936 916 801 635 382 269
186 83 71 53 6
BsaWI w'CCGG_w
Cuts at: 3114 4149 5508 5906 6180 6327 6738 3114
Size: 1035 1359 398 274 147 411 3451
Fragments arranged by size:
3451 1359 1035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2170 2200 2240 2270 3848
Size: 30 165 30 767 30 40 30 1578
Cuts at: 3848 3878 1178
Size: 30 4375
Fragments arranged by size:
4375 1578 767 165 40 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2259 2356 2417 2648 3413 3416 3998
Size: 989 84 97 61 231 765 3 582
Cuts at: 3998 4001 4153 1186
Size: 3 152 4108
Fragments arranged by size:
4108 989 765 582 231 152 97 84
61 3 3
BseYI C'CCAG_C
Cuts at: 3678 6278 6774 3678
Size: 2600 496 3979
Fragments arranged by size:
3979 2600 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 3263 1163
Size: 573 1122 405 4975
Fragments arranged by size:
4975 1122 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4525 5286 6314 1540
Size: 275 186 2524 761 1028 2301
Fragments arranged by size:
2524 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2128 2320 2856 3477 5136 5221 6292 2128
Size: 192 536 621 1659 85 1071 2911
Fragments arranged by size:
2911 1659 1071 621 536 192 85
BsiWI C'GTAC_G
Cuts at: 3147 3147
Size: 7075
BsmI GAATG_Cn'
Cuts at: 4586 4679 4586
Size: 93 6982
BsmBI CGTCTCn'nnnn_
Cuts at: 2786 3382 4060 4246 2786
Size: 596 678 186 5615
Fragments arranged by size:
5615 678 596 186
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2320 2856 3477 4394 5136 5221
Size: 399 338 192 536 621 917 742 85
Cuts at: 5221 6292 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 917 742 621 536 399 338
192 85
BspEI T'CCGG_A
Cuts at: 3114 4149 6738 3114
Size: 1035 2589 3451
Fragments arranged by size:
3451 2589 1035
BspHI T'CATG_A
Cuts at: 3735 4964 6694 3735
Size: 1229 1730 4116
Fragments arranged by size:
4116 1730 1229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 2598 4430 844
Size: 1678 76 1832 3489
Fragments arranged by size:
3489 1832 1678 76
BsrBI CCG'CTC
Cuts at: 1394 1823 2677 4962 1394
Size: 429 854 2285 3507
Fragments arranged by size:
3507 2285 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3510 4023 5570 5744 65
Size: 1183 2262 513 1547 174 1396
Fragments arranged by size:
2262 1547 1396 1183 513 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 2714 3249 5719 5906 1700
Size: 110 285 619 535 2470 187 2869
Fragments arranged by size:
2869 2470 619 535 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 3726 96
Size: 3630 3445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7075
BssSI C'ACGA_G
Cuts at: 2855 2886 3656 5135 6147 2855
Size: 31 770 1479 1012 3783
Fragments arranged by size:
3783 1479 1012 770 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3259 3286 794
Size: 2465 27 4583
Fragments arranged by size:
4583 2465 27
BstBI TT'CG_AA
Cuts at: 5879 5879
Size: 7075
BstEII G'GTnAC_C
Cuts at: 1184 2914 3923 1184
Size: 1730 1009 4336
Fragments arranged by size:
4336 1730 1009
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2206 3673 3919 2026
Size: 180 1467 246 5182
Fragments arranged by size:
5182 1467 246 180
BstYI r'GATC_y
Cuts at: 1972 2568 3069 3538 4405 4772 5157 5174 6626
Size: 596 501 469 867 367 385 17 1452
Cuts at: 6626 6712 6724 7070 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 867 596 501 469 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2271 2271
Size: 7075
BtgI C'CryG_G
Cuts at: 513 1065 2205 2919 3198 3666 3717 513
Size: 552 1140 714 279 468 51 3871
Fragments arranged by size:
3871 1140 714 552 468 279 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2650 2677 2692 487
Size: 683 56 1424 27 15 4870
Fragments arranged by size:
4870 1424 683 56 27 15
BtsI GCAGTG_nn'
Cuts at: 2231 3894 4591 5385 5405 2231
Size: 1663 697 794 20 3901
Fragments arranged by size:
3901 1663 794 697 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7040
DraI TTT'AAA
Cuts at: 4379 4379
Size: 7075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6082 809
Size: 507 4766 1802
Fragments arranged by size:
4766 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2922 3045
Size: 54 1425 50 275 18 495 597 123
Cuts at: 3045 3663 4173 4522 5411 7040 8
Size: 618 510 349 889 1629 43
Fragments arranged by size:
1629 1425 889 618 597 510 495 349
275 123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4522 1537
Size: 275 2710 4090
Fragments arranged by size:
4090 2710 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2114 2328 2583 3905 5005 1353
Size: 731 30 214 255 1322 1100 3423
Fragments arranged by size:
3423 1322 1100 731 255 214 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 3080 5662 6036 6182 387
Size: 1064 138 525 966 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 966 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2381 2607 3023 3095 3290 3788
Size: 393 527 222 226 416 72 195 498
Cuts at: 3788 3862 5138 5735 6522 6790 1239
Size: 74 1276 597 787 268 1524
Fragments arranged by size:
1524 1276 787 597 527 498 416 393
268 226 222 195 74 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2522 2522
Size: 7075
EcoO109I rG'GnC_Cy
Cuts at: 2745 4265 2745
Size: 1520 5555
EcoRI G'AATT_C
Cuts at: 4355 4384 4762 4355
Size: 29 378 6668
Fragments arranged by size:
6668 378 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2764 3614 2389
Size: 375 850 5850
Fragments arranged by size:
5850 850 375
FspI TGC'GCA
Cuts at: 5581 5581
Size: 7075
HaeII r_GCGC'y
Cuts at: 1678 2452 2660 2721 4084 6222 1678
Size: 774 208 61 1363 2138 2531
Fragments arranged by size:
2531 2138 1363 774 208 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2170 2202 2240
Size: 32 135 32 445 32 121 32 38
Cuts at: 2240 2272 2279 2311 3530 3562 3848 3880 5716
Size: 32 7 32 1219 32 286 32 1836
Cuts at: 5716 5748 5790 5822 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1836 1219 445 286 135 121 42
38 32 32 32 32 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 4419 4665 669
Size: 988 281 790 1691 246 3079
Fragments arranged by size:
3079 1691 988 790 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7075
HpaI GTT'AAC
Cuts at: 4665 4665
Size: 7075
KasI G'GCGC_C
Cuts at: 2448 2717 4080 2448
Size: 269 1363 5443
Fragments arranged by size:
5443 1363 269
KpnI G_GTAC'C
Cuts at: 4400 4400
Size: 7075
MfeI C'AATT_G
Cuts at: 4674 4674
Size: 7075
MluI A'CGCG_T
Cuts at: 6951 6951
Size: 7075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 2523 4213 6189 6373 1305
Size: 933 285 1690 1976 184 2007
Fragments arranged by size:
2007 1976 1690 933 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 3665 10
Size: 54 1425 343 495 1338 3420
Fragments arranged by size:
3420 1425 1338 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3185 3497 3722 5033 5392 5551 518
Size: 648 2019 312 225 1311 359 159 2042
Fragments arranged by size:
2042 2019 1311 648 359 312 225 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2842 3037 3043 3277 4491 5168 6316
Size: 717 519 195 6 234 1214 677 1148
Cuts at: 6316 6561 1606
Size: 245 2120
Fragments arranged by size:
2120 1214 1148 717 677 519 245 234
195 6
NaeI GCC'GGC
Cuts at: 2097 2716 2097
Size: 619 6456
NarI GG'CG_CC
Cuts at: 2449 2718 4081 2449
Size: 269 1363 5443
Fragments arranged by size:
5443 1363 269
NcoI C'CATG_G
Cuts at: 513 1065 2205 3666 3717 513
Size: 552 1140 1461 51 3871
Fragments arranged by size:
3871 1461 1140 552 51
NdeI CA'TA_TG
Cuts at: 387 3294 387
Size: 2907 4168
NgoMIV G'CCGG_C
Cuts at: 2095 2714 2095
Size: 619 6456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7075
NotI GC'GGCC_GC
Cuts at: 4522 4522
Size: 7075
NsiI A_TGCA'T
Cuts at: 4339 4339
Size: 7075
NspI r_CATG'y
Cuts at: 1132 1229 2817 2965 3325 3691 3727 4021 4433
Size: 97 1588 148 360 366 36 294 412
Cuts at: 4433 5978 1132
Size: 1545 2229
Fragments arranged by size:
2229 1588 1545 412 366 360 294 148
97 36
PasI CC'CwG_GG
Cuts at: 1348 3673 1348
Size: 2325 4750
PciI A'CATG_T
Cuts at: 3321 5974 3321
Size: 2653 4422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3718 1108
Size: 2610 4465
PfoI T'CCnGG_A
Cuts at: 2136 3125 4307 4359 2136
Size: 989 1182 52 4852
Fragments arranged by size:
4852 1182 989 52
PmeI GTTT'AAAC
Cuts at: 4379 4379
Size: 7075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5144 5176 6657 6689 5144
Size: 32 1481 32 5530
Fragments arranged by size:
5530 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3157 3374 4238 1613
Size: 1544 217 864 4450
Fragments arranged by size:
4450 1544 864 217
PsiI TTA'TAA
Cuts at: 4645 4645
Size: 7075
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 7043
PstI C_TGCA'G
Cuts at: 830 2433 3042 3453 3546 3863 4089 4427 830
Size: 1603 609 411 93 317 226 338 3478
Fragments arranged by size:
3478 1603 609 411 338 317 226 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7075
PvuII CAG'CTG
Cuts at: 2323 2842 3043 2323
Size: 519 201 6355
Fragments arranged by size:
6355 519 201
SalI G'TCGA_C
Cuts at: 1936 4417 1936
Size: 2481 4594
SapI GCTCTTCn'nnn_
Cuts at: 2328 2328
Size: 7075
SbfI CC_TGCA'GG
Cuts at: 4427 4427
Size: 7075
ScaI AGT'ACT
Cuts at: 5323 7035 5323
Size: 1712 5363
SfcI C'TryA_G
Cuts at: 826 1045 2429 3038 3449 3542 3859 4085 4423
Size: 219 1384 609 411 93 317 226 338
Cuts at: 4423 5558 6239 6430 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1384 1135 681 609 411 338 317
226 219 191 93
SfoI GGC'GCC
Cuts at: 2450 2719 4082 2450
Size: 269 1363 5443
Fragments arranged by size:
5443 1363 269
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7075
SgrAI Cr'CCGG_yG
Cuts at: 1700 2714 1700
Size: 1014 6061
SmaI CCC'GGG
Cuts at: 1872 3395 4263 4402 1872
Size: 1523 868 139 4545
Fragments arranged by size:
4545 1523 868 139
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5179 6080 6342 820
Size: 197 926 3236 901 262 1553
Fragments arranged by size:
3236 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4866 493
Size: 4373 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7075
SphI G_CATG'C
Cuts at: 1132 2817 4433 1132
Size: 1685 1616 3774
Fragments arranged by size:
3774 1685 1616
SspI AAT'ATT
Cuts at: 5 52 4999 5
Size: 47 4947 2081
Fragments arranged by size:
4947 2081 47
StuI AGG'CCT
Cuts at: 1517 2528 2639 3918 1517
Size: 1011 111 1279 4674
Fragments arranged by size:
4674 1279 1011 111
StyI C'CwwG_G
Cuts at: 513 1065 2019 2205 2328 2553 2739 3420 3457
Size: 552 954 186 123 225 186 681 37
Cuts at: 3457 3666 3717 3816 4059 4514 513
Size: 209 51 99 243 455 3074
Fragments arranged by size:
3074 954 681 552 455 243 225 209
186 186 123 99 51 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 5102 5119 5272 5457 5902 325
Size: 1255 2331 1191 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 1191 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 3078 3621
Size: 274 80 33 51 1443 648 453 543
Cuts at: 3621 3726 5321 7033 96
Size: 105 1595 1712 138
Fragments arranged by size:
1712 1595 1443 648 543 453 274 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 7035 7067 2118
Size: 32 4885 32 2126
Fragments arranged by size:
4885 2126 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7075
XbaI T'CTAG_A
Cuts at: 4411 4411
Size: 7075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 4102 2320
Size: 1782 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7075
XmaI C'CCGG_G
Cuts at: 1870 3393 4261 4400 1870
Size: 1523 868 139 4545
Fragments arranged by size:
4545 1523 868 139
XmnI GAAnn'nnTTC
Cuts at: 5204 5204
Size: 7075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2725 4883 276
Size: 53 83 186 936 1191 2158 2468
Fragments arranged by size:
2468 2158 1191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BstZ17I DraIII EcoICRI
EcoRV FseI FspAI NruI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI