Restriction map for
pFN21AE2370
(Circular) (Six-base) MAPSORT of: pFN21AE2370.seq Check: 769 from: 1 to: 5224
pFN21AE2370 5224 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5224
AlwNI CAG_nnn'CTG
Cuts at: 2967 4539 2967
Size: 1572 3652
ApaI G_GGCC'C
Cuts at: 2052 2052
Size: 5224
ApaLI G'TGCA_C
Cuts at: 3281 4437 3281
Size: 1156 4068
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 1870
Size: 73 606 4545
Fragments arranged by size:
4545 606 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 4000 618
Size: 325 217 627 758 1455 1842
Fragments arranged by size:
1842 1455 758 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2543 1391
Size: 661 491 4072
Fragments arranged by size:
4072 661 491
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3835 136
Size: 107 122 71 631 605 2163 1525
Fragments arranged by size:
2163 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2641 2641
Size: 5224
Bme1580I G_kGCm'C
Cuts at: 1790 2052 3285 4441 1790
Size: 262 1233 1156 2573
Fragments arranged by size:
2573 1233 1156 262
BmgBI CAC'GTC
Cuts at: 1318 2011 1318
Size: 693 4531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2955 3913 442
Size: 416 53 83 399 24 1538 958 1753
Fragments arranged by size:
1753 1538 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3884 1239
Size: 2645 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3031 3413 4048 275
Size: 53 83 186 936 1498 382 635 1451
Fragments arranged by size:
1498 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3657 4055 4329 4476 4887 3657
Size: 398 274 147 411 3994
Fragments arranged by size:
3994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2497 2527 1178
Size: 30 165 30 1094 30 3875
Fragments arranged by size:
3875 1094 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 1186
Size: 1324 3900
BseYI C'CCAG_C
Cuts at: 2060 4427 4923 2060
Size: 2367 496 2361
Fragments arranged by size:
2367 2361 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 1163
Size: 573 555 4096
Fragments arranged by size:
4096 573 555
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 2674 3435 4463 1540
Size: 275 186 205 468 761 1028 2301
Fragments arranged by size:
2301 1028 761 468 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 3285 3370 4441 3285
Size: 85 1071 4068
Fragments arranged by size:
4068 1071 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2543 3285 3370 4441 1391
Size: 399 262 491 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 262 85
BspEI T'CCGG_A
Cuts at: 4887 4887
Size: 5224
BspHI T'CATG_A
Cuts at: 3113 4843 3113
Size: 1730 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2579 844
Size: 1274 461 3489
Fragments arranged by size:
3489 1274 461
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 1394
Size: 429 1288 3507
Fragments arranged by size:
3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 3868 4055 1700
Size: 110 254 1804 187 2869
Fragments arranged by size:
2869 1804 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5224
BssSI C'ACGA_G
Cuts at: 3284 4296 3284
Size: 1012 4212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5224
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5224
BstYI r'GATC_y
Cuts at: 1972 2289 2439 2554 2921 3306 3323 4775 4861
Size: 317 150 115 367 385 17 1452 86
Cuts at: 4861 4873 5219 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 317 150 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2293 2323 513
Size: 552 939 289 30 3414
Fragments arranged by size:
3414 939 552 289 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4485
Fragments arranged by size:
4485 683 56
BtsI GCAGTG_nn'
Cuts at: 2740 3534 3554 2740
Size: 794 20 4410
Fragments arranged by size:
4410 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2216 4231 809
Size: 507 900 2015 1802
Fragments arranged by size:
2015 1802 900 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3560 5189
Size: 54 1425 50 275 18 841 889 1629
Cuts at: 5189 8
Size: 43
Fragments arranged by size:
1629 1425 889 841 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 3154 1353
Size: 812 989 3423
Fragments arranged by size:
3423 989 812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3811 4185 4331 387
Size: 1064 138 2222 374 146 1280
Fragments arranged by size:
2222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3287 3884 4671 4939 1239
Size: 393 1655 597 787 268 1524
Fragments arranged by size:
1655 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2048 2049 2092 2254 2048
Size: 1 43 162 5018
Fragments arranged by size:
5018 162 43 1
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 3730 3730
Size: 5224
HaeII r_GCGC'y
Cuts at: 1678 2314 4371 1678
Size: 636 2057 2531
Fragments arranged by size:
2531 2057 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 3865 3897 3939
Size: 32 135 32 611 32 1650 32 42
Cuts at: 3939 3971 1373
Size: 32 2626
Fragments arranged by size:
2626 1650 611 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 2394 4338 4522 1305
Size: 921 168 1944 184 2007
Fragments arranged by size:
2007 1944 921 184 168
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2483 3182 3541 3700 518
Size: 648 843 474 699 359 159 2042
Fragments arranged by size:
2042 843 699 648 474 359 159
MspA1I CmG'CkG
Cuts at: 1606 2640 3317 4465 4710 1606
Size: 1034 677 1148 245 2120
Fragments arranged by size:
2120 1148 1034 677 245
NaeI GCC'GGC
Cuts at: 2066 2066
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NgoMIV G'CCGG_C
Cuts at: 2064 2064
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2419 2582 4127 1132
Size: 97 1190 163 1545 2229
Fragments arranged by size:
2229 1545 1190 163 97
PasI CC'CwG_GG
Cuts at: 1348 2044 1348
Size: 696 4528
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PspOMI G'GGCC_C
Cuts at: 2048 2048
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2576 830
Size: 1746 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SfcI C'TryA_G
Cuts at: 826 1045 2572 3707 4388 4579 826
Size: 219 1527 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2551 1872
Size: 679 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2419 2582 1132
Size: 1287 163 3774
Fragments arranged by size:
3774 1287 163
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3074
Fragments arranged by size:
3074 939 659 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 3251 3268 3421 3606 4051 325
Size: 1255 897 774 17 153 185 445 1498
Fragments arranged by size:
1498 1255 897 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3470 5182 96
Size: 274 80 33 51 1443 1493 1712 138
Fragments arranged by size:
1712 1493 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2497 2529 5184 5216 2497
Size: 32 2655 32 2505
Fragments arranged by size:
2655 2505 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5224
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2549 1870
Size: 679 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 3353 2240
Size: 1113 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI