Restriction map for
pFN21AE2366
(Circular) (Six-base) MAPSORT of: pFN21AE2366.seq Check: 9624 from: 1 to: 6103
pFN21AE2366 6103 bp
With 182 enzymes: SgfI *
August 4, 2011 13:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2024 3446 1937
Size: 87 1422 4594
Fragments arranged by size:
4594 1422 87
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4166 5550 5818 1632
Size: 2534 1384 268 1917
Fragments arranged by size:
2534 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 2678 1166
Size: 1512 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2071 3846 5418 2071
Size: 1775 1572 2756
Fragments arranged by size:
2756 1775 1572
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 2594 3412 3638 3790 5899 1493
Size: 1101 818 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 818 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 3428 1870
Size: 73 1485 4545
Fragments arranged by size:
4545 1485 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3424 4879 618
Size: 325 217 627 1637 1455 1842
Fragments arranged by size:
1842 1637 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3422 1391
Size: 2031 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 2072 3520 2072
Size: 1448 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2709 4164 5320 1790
Size: 919 1455 1156 2573
Fragments arranged by size:
2573 1455 1156 919
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 3834 4792
Size: 416 53 83 399 24 1691 726 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1753 1691 958 726 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2502 2502
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 4763 1239
Size: 888 2636 2579
Fragments arranged by size:
2636 2579 888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 4228 5093 5391 2252
Size: 1976 865 298 2964
Fragments arranged by size:
2964 1976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4766 882
Size: 617 3267 2219
Fragments arranged by size:
3267 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2419 3894 493
Size: 711 1215 1475 2702
Fragments arranged by size:
2702 1475 1215 711
BsaBI GATnn'nnATC
Cuts at: 2613 2736 2844 6097 2613
Size: 123 108 3253 2619
Fragments arranged by size:
3253 2619 123 108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3910 4292 4927 275
Size: 53 83 186 936 2377 382 635 1451
Fragments arranged by size:
2377 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2400 4536 4934 5208 5355 5766 2400
Size: 2136 398 274 147 411 2737
Fragments arranged by size:
2737 2136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5878
Fragments arranged by size:
5878 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6103
BseYI C'CCAG_C
Cuts at: 3387 5306 5802 3387
Size: 1919 496 3688
Fragments arranged by size:
3688 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4164 4249 5320 4164
Size: 85 1071 4947
Fragments arranged by size:
4947 1071 85
BsmI GAATG_Cn'
Cuts at: 2824 3348 3614 3707 2824
Size: 524 266 93 5220
Fragments arranged by size:
5220 524 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2103
Size: 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2709 3422 4164 4249 5320 1391
Size: 399 919 713 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 713 399 85
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3458 844
Size: 2614 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3008 4598 4772 65
Size: 1183 1760 1590 174 1396
Fragments arranged by size:
1760 1590 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4747 4934 1700
Size: 110 222 2715 187 2869
Fragments arranged by size:
2869 2715 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 3281 96
Size: 3185 2918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6103
BssSI C'ACGA_G
Cuts at: 4163 5175 4163
Size: 1012 5091
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3205 794
Size: 2411 3692
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 2329 2504 2608 3013 3262 3433 3800 4185
Size: 357 175 104 405 249 171 367 385
Cuts at: 4185 4202 5654 5740 5752 6098 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 405 385 367 357 346 249
175 171 104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 4047
Fragments arranged by size:
4047 1504 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2956 487
Size: 683 56 1730 3634
Fragments arranged by size:
3634 1730 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2552 3619 4413 4433 2083
Size: 469 1067 794 20 3753
Fragments arranged by size:
3753 1067 794 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2566 2974
Size: 54 1425 50 275 18 225 511 408
Cuts at: 2974 3550 4439 6068 8
Size: 576 889 1629 43
Fragments arranged by size:
1629 1425 889 576 511 408 275 225
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4033 1353
Size: 2680 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 5064 5210 387
Size: 1064 138 3101 374 146 1280
Fragments arranged by size:
3101 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 4166 4763 5550 5818 1239
Size: 393 495 2039 597 787 268 1524
Fragments arranged by size:
2039 1524 787 597 495 393 268
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 3388 5250 1678
Size: 1710 1862 2531
Fragments arranged by size:
2531 1862 1710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 3005
Size: 32 135 32 943 32 112 32 314
Cuts at: 3005 3037 4744 4776 4818 4850 1373
Size: 32 1707 32 42 32 2626
Fragments arranged by size:
2626 1707 943 314 135 112 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 3447 3693 669
Size: 988 281 87 1422 246 3079
Fragments arranged by size:
3079 1422 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2568 10
Size: 54 1425 343 736 3545
Fragments arranged by size:
3545 1425 736 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2678 2712 3303 4061 4420 4579
Size: 648 1408 104 34 591 758 359 159
Cuts at: 4579 518
Size: 2042
Fragments arranged by size:
2042 1408 758 648 591 359 159 104
34
MspA1I CmG'CkG
Cuts at: 1606 3519 4196 5344 5589 1606
Size: 1913 677 1148 245 2120
Fragments arranged by size:
2120 1913 1148 677 245
NaeI GCC'GGC
Cuts at: 2034 2034
Size: 6103
NcoI C'CATG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 4047
Fragments arranged by size:
4047 1504 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NgoMIV G'CCGG_C
Cuts at: 2032 2032
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2050 3461 5006 1132
Size: 97 821 1411 1545 2229
Fragments arranged by size:
2229 1545 1411 821 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2294 3381 1108
Size: 1186 1087 3830
Fragments arranged by size:
3830 1186 1087
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2703 2735 4172 4204 5685 5717 2703
Size: 32 1437 32 1481 32 3089
Fragments arranged by size:
3089 1481 1437 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 2844 3673 2844
Size: 829 5274
PstI C_TGCA'G
Cuts at: 830 3455 830
Size: 2625 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 2023 3445 1936
Size: 87 1422 4594
Fragments arranged by size:
4594 1422 87
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SfcI C'TryA_G
Cuts at: 826 1045 2349 2496 3451 4586 5267 5458 826
Size: 219 1304 147 955 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 955 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 5771
SmaI CCC'GGG
Cuts at: 1872 3430 1872
Size: 1558 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 4207 5108 5370 820
Size: 197 926 324 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 2243 2688 4027 5
Size: 47 2191 445 1339 2081
Fragments arranged by size:
2191 2081 1339 445 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6103
StyI C'CwwG_G
Cuts at: 513 1065 2569 3542 513
Size: 552 1504 973 3074
Fragments arranged by size:
3074 1504 973 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 4130 4147 4300 4485 4930 325
Size: 1255 1155 1395 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3281 4349 6061
Size: 274 80 33 51 1443 1304 1068 1712
Cuts at: 6061 96
Size: 138
Fragments arranged by size:
1712 1443 1304 1068 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 6071
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 1316
Size: 810 5293
XbaI T'CTAG_A
Cuts at: 2507 3439 2507
Size: 932 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3428 1870
Size: 1558 4545
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI