Restriction map for
pFN21AE2363
(Circular) (Six-base) MAPSORT of: pFN21AE2363.seq Check: 7393 from: 1 to: 6256
pFN21AE2363 6256 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2729 3443 2729
Size: 714 5542
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 3599 1937
Size: 418 1244 4594
Fragments arranged by size:
4594 1244 418
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 4319 5703 5971 1632
Size: 600 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 600 268
AflII C'TTAA_G
Cuts at: 820 1017 3500 820
Size: 197 2483 3576
Fragments arranged by size:
3576 2483 197
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 2786 5087 2786
Size: 2301 3955
AhdI GACnn_n'nnGTC
Cuts at: 4985 4985
Size: 6256
AleI CACnn'nnGTG
Cuts at: 1166 2798 1166
Size: 1632 4624
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3274 3999 5571 3274
Size: 725 1572 3959
Fragments arranged by size:
3959 1572 725
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 2369 3011 3354 3581 1870
Size: 73 310 116 642 343 227 4545
Fragments arranged by size:
4545 642 343 310 227 116 73
AvrII C'CTAG_G
Cuts at: 3115 3115
Size: 6256
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2283 3577 5032 618
Size: 325 217 627 233 263 1294 1455 1842
Fragments arranged by size:
1842 1455 1294 627 325 263 233 217
BanII G_rGCy'C
Cuts at: 1391 2529 2876 3575 1391
Size: 1138 347 699 4072
Fragments arranged by size:
4072 1138 699 347
BbeI G_GCGC'C
Cuts at: 2287 2287
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 928
Size: 971 266 5019
Fragments arranged by size:
5019 971 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4447 4481 1903
Size: 34 50 34 2426 34 3678
Fragments arranged by size:
3678 2426 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2043 4867 136
Size: 107 122 71 631 605 371 2824 1525
Fragments arranged by size:
2824 1525 631 605 371 122 107 71
BglII A'GATC_T
Cuts at: 3252 6251 3252
Size: 2999 3257
BlpI GC'TnA_GC
Cuts at: 2033 3673 2033
Size: 1640 4616
Bme1580I G_kGCm'C
Cuts at: 1790 2205 4317 5473 1790
Size: 415 2112 1156 2573
Fragments arranged by size:
2573 2112 1156 415
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3987 4945 442
Size: 416 53 83 399 24 2570 958 1753
Fragments arranged by size:
2570 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2876 3377 4916 1239
Size: 785 852 501 1539 2579
Fragments arranged by size:
2579 1539 852 785 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 4381 5246 5544 2825
Size: 1556 865 298 3537
Fragments arranged by size:
3537 1556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2366 4919 882
Size: 617 867 2553 2219
Fragments arranged by size:
2553 2219 867 617
BsaAI yAC'GTr
Cuts at: 493 1204 2206 4047 493
Size: 711 1002 1841 2702
Fragments arranged by size:
2702 1841 1002 711
BsaBI GATnn'nnATC
Cuts at: 2232 6250 2232
Size: 4018 2238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 4063 4445 5080
Size: 53 83 186 936 751 1779 382 635
Cuts at: 5080 275
Size: 1451
Fragments arranged by size:
1779 1451 936 751 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2786 4689 5087 5361 5508 5919 2786
Size: 1903 398 274 147 411 3123
Fragments arranged by size:
3123 1903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6031
Fragments arranged by size:
6031 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2053 3146 3263 3311 1186
Size: 867 1093 117 48 4131
Fragments arranged by size:
4131 1093 867 117 48
BseYI C'CCAG_C
Cuts at: 2457 2754 2860 2876 3072 3484 5459 5955 2457
Size: 297 106 16 196 412 1975 496 2758
Fragments arranged by size:
2758 1975 496 412 297 196 106 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 3287 1163
Size: 573 1506 45 4132
Fragments arranged by size:
4132 1506 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4317 4402 5473 4317
Size: 85 1071 5100
Fragments arranged by size:
5100 1071 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2529 2876 3575 4317 4402 5473
Size: 399 415 324 347 699 742 85 1071
Cuts at: 5473 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 699 415 399 347 324
85
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 3443 3611 844
Size: 1885 714 168 3489
Fragments arranged by size:
3489 1885 714 168
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 2344 4143 1394
Size: 429 482 39 1799 3507
Fragments arranged by size:
3507 1799 482 429 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2310 2786 4900 5087 1700
Size: 110 500 476 2114 187 2869
Fragments arranged by size:
2869 2114 500 476 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2216 3526 1184
Size: 1032 1310 3914
Fragments arranged by size:
3914 1310 1032
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 2800 2491
Size: 309 5947
BstYI r'GATC_y
Cuts at: 1972 3252 3358 3586 3953 4338 4355 5807 5893
Size: 1280 106 228 367 385 17 1452 86
Cuts at: 5893 5905 6251 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1280 385 367 346 228 106
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3084 3084
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2279 2490 2555 2913 3021 513
Size: 552 1214 211 65 358 108 3748
Fragments arranged by size:
3748 1214 552 358 211 108 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2734 487
Size: 683 56 1508 4009
Fragments arranged by size:
4009 1508 683 56
BtsI GCAGTG_nn'
Cuts at: 2927 3772 4566 4586 2927
Size: 845 794 20 4597
Fragments arranged by size:
4597 845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 2542 2745
Size: 54 1425 50 275 18 667 45 203
Cuts at: 2745 2889 3133 3703 4592 6221 8
Size: 144 244 570 889 1629 43
Fragments arranged by size:
1629 1425 889 667 570 275 244 203
144 54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4186 1353
Size: 2833 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 3180 4843 5217 5363 387
Size: 1064 138 666 925 1663 374 146 1280
Fragments arranged by size:
1663 1280 1064 925 666 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2232 2876 3377 4319 4916 5703
Size: 393 392 208 644 501 942 597 787
Cuts at: 5703 5971 1239
Size: 268 1524
Fragments arranged by size:
1524 942 787 644 597 501 393 392
268 208
EcoNI CCTnn'n_nnAGG
Cuts at: 2678 2678
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2274 3080 2274
Size: 806 5450
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 6256
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2287 5403 1678
Size: 609 3116 2531
Fragments arranged by size:
3116 2531 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4897 4929 4971 5003 1373
Size: 32 135 32 3325 32 42 32 2626
Fragments arranged by size:
3325 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2176 2770 3600 3846 669
Size: 988 281 238 594 830 246 3079
Fragments arranged by size:
3079 988 830 594 281 246 238
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2283 2283
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2564 2859 5370 5554 1305
Size: 1259 295 2511 184 2007
Fragments arranged by size:
2511 2007 1259 295 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 2544 3135 10
Size: 54 1425 343 667 45 591 3131
Fragments arranged by size:
3131 1425 667 591 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2489 2763 2798 4214 4573 4732
Size: 648 1269 54 274 35 1416 359 159
Cuts at: 4732 518
Size: 2042
Fragments arranged by size:
2042 1416 1269 648 359 274 159 54
35
MspA1I CmG'CkG
Cuts at: 1606 2119 2825 3672 4349 5497 5742 1606
Size: 513 706 847 677 1148 245 2120
Fragments arranged by size:
2120 1148 847 706 677 513 245
NaeI GCC'GGC
Cuts at: 2312 2312
Size: 6256
NarI GG'CG_CC
Cuts at: 2284 2284
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 2279 2490 513
Size: 552 1214 211 4279
Fragments arranged by size:
4279 1214 552 211
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NgoMIV G'CCGG_C
Cuts at: 2310 2310
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2912 3614 5159 1132
Size: 97 1683 702 1545 2229
Fragments arranged by size:
2229 1683 1545 702 97
PasI CC'CwG_GG
Cuts at: 1348 2699 1348
Size: 1351 4905
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2761 1108
Size: 1653 4603
PfoI T'CCnGG_A
Cuts at: 2972 2972
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2274 2274
Size: 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PstI C_TGCA'G
Cuts at: 830 3608 830
Size: 2778 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
PvuII CAG'CTG
Cuts at: 2119 2825 2119
Size: 706 5550
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 2274 2274
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SexAI A'CCwGG_T
Cuts at: 3323 3323
Size: 6256
SfcI C'TryA_G
Cuts at: 826 1045 3604 4739 5420 5611 826
Size: 219 2559 1135 681 191 1471
Fragments arranged by size:
2559 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2285 2285
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2255 3356 3583 1872
Size: 383 1101 227 4545
Fragments arranged by size:
4545 1101 383 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2369 2840 3011 3500 4360 5261
Size: 197 926 426 471 171 489 860 901
Cuts at: 5261 5523 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 860 489 471 426 262
197 171
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 2912 3614 1132
Size: 1780 702 3774
Fragments arranged by size:
3774 1780 702
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 3506 1517
Size: 1989 4267
StyI C'CwwG_G
Cuts at: 513 1065 2279 2376 2412 2490 2568 3115 3695
Size: 552 1214 97 36 78 78 547 580
Cuts at: 3695 513
Size: 3074
Fragments arranged by size:
3074 1214 580 552 547 97 78 78
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2852 3086 3398 3476 4283 4300 4453
Size: 1255 1272 234 312 78 807 17 153
Cuts at: 4453 4638 5083 325
Size: 185 445 1498
Fragments arranged by size:
1498 1272 1255 807 445 312 234 185
153 78 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3375 4502 6214
Size: 274 80 33 51 1443 1398 1127 1712
Cuts at: 6214 96
Size: 138
Fragments arranged by size:
1712 1443 1398 1127 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6256
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 2884 3331 3446 2822
Size: 62 447 115 5632
Fragments arranged by size:
5632 447 115 62
XhoI C'TCGA_G
Cuts at: 1943 2369 3011 1943
Size: 426 642 5188
Fragments arranged by size:
5188 642 426
XmaI C'CCGG_G
Cuts at: 1870 2253 3354 3581 1870
Size: 383 1101 227 4545
Fragments arranged by size:
4545 1101 383 227
XmnI GAAnn'nnTTC
Cuts at: 2162 2393 4385 2162
Size: 231 1992 4033
Fragments arranged by size:
4033 1992 231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SapI SfiI SrfI SwaI