Restriction map for
pFN21AE2360
(Circular) (Six-base) MAPSORT of: pFN21AE2360.seq Check: 2103 from: 1 to: 10924
pFN21AE2360 10924 bp
With 182 enzymes: SgfI *
September 13, 2011 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5638 5638
Size: 10924
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8734 278
Size: 53 83 186 936 7198 2468
Fragments arranged by size:
7198 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2261 3255 5356 6548 8267 1937
Size: 324 994 2101 1192 1719 4594
Fragments arranged by size:
4594 2101 1719 1192 994 324
Acc65I G'GTAC_C
Cuts at: 4282 8245 4282
Size: 3963 6961
AclI AA'CG_TT
Cuts at: 1563 3100 7135 9051 9424 1563
Size: 1537 4035 1916 373 3063
Fragments arranged by size:
4035 3063 1916 1537 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5117 5369 6089 6183 6231 6372 6393 7279
Size: 3485 252 720 94 48 141 21 886
Cuts at: 7279 8238 8987 10371 10639 1632
Size: 959 749 1384 268 1917
Fragments arranged by size:
3485 1917 1384 959 886 749 720 268
252 141 94 48 21
AfeI AGC'GCT
Cuts at: 5102 5102
Size: 10924
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10727
AflIII A'CryG_T
Cuts at: 1203 6367 6435 7959 8155 9823 10800 1203
Size: 5164 68 1524 196 1668 977 1327
Fragments arranged by size:
5164 1668 1524 1327 977 196 68
AgeI A'CCGG_T
Cuts at: 9755 9755
Size: 10924
AhdI GACnn_n'nnGTC
Cuts at: 9653 9653
Size: 10924
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2270 7887 1184
Size: 1086 5617 4221
Fragments arranged by size:
5617 4221 1086
AlwNI CAG_nnn'CTG
Cuts at: 4076 4327 6209 6917 8667 10239 4076
Size: 251 1882 708 1750 1572 4761
Fragments arranged by size:
4761 1882 1750 1572 708 251
ApaI G_GGCC'C
Cuts at: 2293 2293
Size: 10924
ApaLI G'TGCA_C
Cuts at: 3530 4487 8981 10137 3530
Size: 957 4494 1156 4317
Fragments arranged by size:
4494 4317 1156 957
ApoI r'AATT_y
Cuts at: 1493 2021 2641 3396 3426 3499 4010 4493 5207
Size: 528 620 755 30 73 511 483 714
Cuts at: 5207 7248 7386 7970 8059 8233 8459 8611 10720
Size: 2041 138 584 89 174 226 152 2109
Cuts at: 10720 1493
Size: 1697
Fragments arranged by size:
2109 2041 1697 755 714 620 584 528
511 483 226 174 152 138 89 73
30
AseI AT'TA_AT
Cuts at: 160 1032 6299 7734 9478 160
Size: 872 5267 1435 1744 1606
Fragments arranged by size:
5267 1744 1606 1435 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10924
AvaI C'yCGr_G
Cuts at: 1870 1943 4959 4976 8249 1870
Size: 73 3016 17 3273 4545
Fragments arranged by size:
4545 3273 3016 73 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5486 5519 6503 6536 5486
Size: 33 984 33 9874
Fragments arranged by size:
9874 984 33 33
BamHI G'GATC_C
Cuts at: 8254 8621 8254
Size: 367 10557
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4282 8245 9700 618
Size: 325 217 627 2495 3963 1455 1842
Fragments arranged by size:
3963 2495 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2096 2130 2293 3189 3296 3335 4551
Size: 673 32 34 163 896 107 39 1216
Cuts at: 4551 5934 6855 8243 1391
Size: 1383 921 1388 4072
Fragments arranged by size:
4072 1388 1383 1216 921 896 673 163
107 39 34 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 3010 3696 7562 8124 928
Size: 971 555 556 686 3866 562 3728
Fragments arranged by size:
3866 3728 971 686 562 556 555
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9115 9149 1903
Size: 34 7178 34 3678
Fragments arranged by size:
7178 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5818 7938 8816 10026 5818
Size: 2120 878 1210 6716
Fragments arranged by size:
6716 2120 1210 878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10924
BfrBI ATG'CAT
Cuts at: 2938 5450 7264 8128 2938
Size: 2512 1814 864 5734
Fragments arranged by size:
5734 2512 1814 864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3330 9535 136
Size: 107 122 71 631 605 1658 6205 1525
Fragments arranged by size:
6205 1658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2191 4477 10919 2191
Size: 2286 6442 2196
Fragments arranged by size:
6442 2286 2196
BlpI GC'TnA_GC
Cuts at: 2036 2058 6823 8341 2036
Size: 22 4765 1518 4619
Fragments arranged by size:
4765 4619 1518 22
Bme1580I G_kGCm'C
Cuts at: 1790 2293 2702 3534 3825 4045 4491 8985 10141
Size: 503 409 832 291 220 446 4494 1156
Cuts at: 10141 1790
Size: 2573
Fragments arranged by size:
4494 2573 1156 832 503 446 409 291
220
BmgBI CAC'GTC
Cuts at: 1318 7816 1318
Size: 6498 4426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5806 8655 9613
Size: 416 53 83 399 24 4389 2849 958
Cuts at: 9613 442
Size: 1753
Fragments arranged by size:
4389 2849 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5091 7923 1056
Size: 4035 2832 4057
Fragments arranged by size:
4057 4035 2832
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2386 3666 4803 5741 2386
Size: 1280 1137 938 7569
Fragments arranged by size:
7569 1280 1137 938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4248 6797 7112 9584 1239
Size: 3009 2549 315 2472 2579
Fragments arranged by size:
3009 2579 2549 2472 315
Bpu10I CC'TnA_GC
Cuts at: 6854 6854
Size: 10924
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2714 3603 3683 4458 7197 7414 9049 9914 10212
Size: 889 80 775 2739 217 1635 865 298
Cuts at: 10212 2714
Size: 3426
Fragments arranged by size:
3426 2739 1635 889 865 775 298 217
80
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2401 9587 882
Size: 617 902 7186 2219
Fragments arranged by size:
7186 2219 902 617
BsaAI yAC'GTr
Cuts at: 493 1204 2344 7696 8715 493
Size: 711 1140 5352 1019 2702
Fragments arranged by size:
5352 2702 1140 1019 711
BsaBI GATnn'nnATC
Cuts at: 4734 5301 6321 10918 4734
Size: 567 1020 4597 4740
Fragments arranged by size:
4740 4597 1020 567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2676 8731 9113 9748
Size: 53 83 186 936 1143 6055 382 635
Cuts at: 9748 275
Size: 1451
Fragments arranged by size:
6055 1451 1143 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2941 9357 9755 10029 10176 10587 2941
Size: 6416 398 274 147 411 3278
Fragments arranged by size:
6416 3278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 1178
Size: 30 165 30 1374 30 9295
Fragments arranged by size:
9295 1374 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3009 3642 4776 7795 1186
Size: 1823 633 1134 3019 4315
Fragments arranged by size:
4315 3019 1823 1134 633
BseYI C'CCAG_C
Cuts at: 4794 7052 10127 10623 4794
Size: 2258 3075 496 5095
Fragments arranged by size:
5095 3075 2258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 3501 4663 5595 6457 6495 7883
Size: 573 540 1225 1162 932 862 38 1388
Cuts at: 7883 1163
Size: 4204
Fragments arranged by size:
4204 1388 1225 1162 932 862 573 540
38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8374 9135 10163 1540
Size: 275 186 6373 761 1028 2301
Fragments arranged by size:
6373 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3534 4491 4551 4786 5654 5934 6855 8985
Size: 1470 957 60 235 868 280 921 2130
Cuts at: 8985 9070 10141 2064
Size: 85 1071 2847
Fragments arranged by size:
2847 2130 1470 1071 957 921 868 280
235 85 60
BsmI GAATG_Cn'
Cuts at: 3582 8128 8435 8528 3582
Size: 4546 307 93 5978
Fragments arranged by size:
5978 4546 307 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2566 2849 2566
Size: 283 10641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2096 2130 2293 2702 3189 3296
Size: 399 274 32 34 163 409 487 107
Cuts at: 3296 3335 3534 3825 4045 4491 4551 4786 5654
Size: 39 199 291 220 446 60 235 868
Cuts at: 5654 5934 6855 8243 8985 9070 10141 1391
Size: 280 921 1388 742 85 1071 2174
Fragments arranged by size:
2174 1388 1071 921 868 742 487 446
409 399 291 280 274 235 220 199
163 107 85 60 39 34 32
BspEI T'CCGG_A
Cuts at: 2941 10587 2941
Size: 7646 3278
BspHI T'CATG_A
Cuts at: 4593 8813 10543 4593
Size: 4220 1730 4974
Fragments arranged by size:
4974 4220 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4075 5540 5638 7855 8279 844
Size: 3231 1465 98 2217 424 3489
Fragments arranged by size:
3489 3231 2217 1465 424 98
BsrBI CCG'CTC
Cuts at: 1394 1823 8011 8811 1394
Size: 429 6188 800 3507
Fragments arranged by size:
6188 3507 800 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3993 4397 4504 5376 7044 7989 9419
Size: 1183 2745 404 107 872 1668 945 1430
Cuts at: 9419 9593 65
Size: 174 1396
Fragments arranged by size:
2745 1668 1430 1396 1183 945 872 404
174 107
BsrFI r'CCGG_y
Cuts at: 1700 1810 9568 9755 1700
Size: 110 7758 187 2869
Fragments arranged by size:
7758 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10924
BssSI C'ACGA_G
Cuts at: 8984 9996 8984
Size: 1012 9912
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4327 6061 794
Size: 3533 1734 5657
Fragments arranged by size:
5657 3533 1734
BstBI TT'CG_AA
Cuts at: 2383 6073 9728 2383
Size: 3690 3655 3579
Fragments arranged by size:
3690 3655 3579
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10924
BstYI r'GATC_y
Cuts at: 1972 2191 4477 4584 5176 8254 8621 9006 9023
Size: 219 2286 107 592 3078 367 385 17
Cuts at: 9023 10475 10561 10573 10919 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3078 2286 1977 1452 592 385 367 346
219 107 86 17 12
BstZ17I GTA'TAC
Cuts at: 6549 6549
Size: 10924
Bsu36I CC'TnA_GG
Cuts at: 4788 5181 5925 4788
Size: 393 744 9787
Fragments arranged by size:
9787 744 393
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 4578 4683 513
Size: 552 939 93 2481 105 6754
Fragments arranged by size:
6754 2481 939 552 105 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6935 487
Size: 683 56 5709 4476
Fragments arranged by size:
5709 4476 683 56
BtsI GCAGTG_nn'
Cuts at: 5003 6762 8440 9234 9254 5003
Size: 1759 1678 794 20 6673
Fragments arranged by size:
6673 1759 1678 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10924
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3163 3198 529
Size: 35 2599 35 8255
Fragments arranged by size:
8255 2599 35 35
DraI TTT'AAA
Cuts at: 4563 6276 6465 7740 8228 4563
Size: 1713 189 1275 488 7259
Fragments arranged by size:
7259 1713 1275 488 189
DraIII CAC_nnn'GTG
Cuts at: 3479 6255 3479
Size: 2776 8148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9931 809
Size: 507 8615 1802
Fragments arranged by size:
8615 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3309 4920
Size: 54 1425 50 275 18 177 1302 1611
Cuts at: 4920 6286 6759 6879 7312 8371 9260 10889 8
Size: 1366 473 120 433 1059 889 1629 43
Fragments arranged by size:
1629 1611 1425 1366 1302 1059 889 473
433 275 177 120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8371 1537
Size: 275 6559 4090
Fragments arranged by size:
6559 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2956 3301 3340 3867 4634 4932 5388 6075
Size: 1603 345 39 527 767 298 456 687
Cuts at: 6075 6421 7074 7233 7697 8854 1353
Size: 346 653 159 464 1157 3423
Fragments arranged by size:
3423 1603 1157 767 687 653 527 464
456 346 345 298 159 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4327 9511 9885 10031 387
Size: 1064 138 2738 5184 374 146 1280
Fragments arranged by size:
5184 2738 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 4549 5932 6853 2062
Size: 2487 1383 921 6133
Fragments arranged by size:
6133 2487 1383 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4248 5117 5369 6089 6183 6231 6372
Size: 393 2616 869 252 720 94 48 141
Cuts at: 6372 6393 6797 7112 7279 8238 8987 9584 10371
Size: 21 404 315 167 959 749 597 787
Cuts at: 10371 10639 1239
Size: 268 1524
Fragments arranged by size:
2616 1524 959 869 787 749 720 597
404 393 315 268 252 167 141 94
48 21
EcoNI CCTnn'n_nnAGG
Cuts at: 6568 6568
Size: 10924
EcoO109I rG'GnC_Cy
Cuts at: 2289 7603 2289
Size: 5314 5610
EcoRI G'AATT_C
Cuts at: 4010 8233 8611 4010
Size: 4223 378 6323
Fragments arranged by size:
6323 4223 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3644 4777 5359 7543 3644
Size: 1133 582 2184 7025
Fragments arranged by size:
7025 2184 1133 582
FspI TGC'GCA
Cuts at: 2018 9430 2018
Size: 7412 3512
HaeII r_GCGC'y
Cuts at: 1678 5104 10071 1678
Size: 3426 4967 2531
Fragments arranged by size:
4967 3426 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 2410 2442 2453
Size: 32 135 32 652 32 154 32 11
Cuts at: 2453 2456 2485 2488 2923 2955 2997 3029 4364
Size: 3 29 3 435 32 42 32 1335
Cuts at: 4364 4396 4844 4876 4972 5004 5141 5173 6035
Size: 32 448 32 96 32 137 32 862
Cuts at: 6035 6067 6224 6256 6378 6410 6562 6594 9565
Size: 32 157 32 122 32 152 32 2971
Cuts at: 9565 9597 9639 9671 1373
Size: 32 42 32 2626
Fragments arranged by size:
2971 2626 1335 862 652 448 435 157
154 152 137 135 122 96 42 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
29 11 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2243 8268 8514 669
Size: 988 281 305 6025 246 3079
Fragments arranged by size:
6025 3079 988 305 281 246
HindIII A'AGCT_T
Cuts at: 748 3677 4952 7796 748
Size: 2929 1275 2844 3876
Fragments arranged by size:
3876 2929 2844 1275
HpaI GTT'AAC
Cuts at: 8514 8514
Size: 10924
KpnI G_GTAC'C
Cuts at: 4286 8249 4286
Size: 3963 6961
MfeI C'AATT_G
Cuts at: 3226 5498 7746 8523 3226
Size: 2272 2248 777 5627
Fragments arranged by size:
5627 2272 2248 777
MluI A'CGCG_T
Cuts at: 10800 10800
Size: 10924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 4789 4914 5497 7858 10038 10222 1305
Size: 1719 1765 125 583 2361 2180 184 2007
Fragments arranged by size:
2361 2180 2007 1765 1719 583 184 125
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3311 4922 6288 6761 6881
Size: 54 1425 343 1479 1611 1366 473 120
Cuts at: 6881 7314 10
Size: 433 3620
Fragments arranged by size:
3620 1611 1479 1425 1366 473 433 343
120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3933 4646 4659 7074 8882 9241 9400
Size: 648 2767 713 13 2415 1808 359 159
Cuts at: 9400 518
Size: 2042
Fragments arranged by size:
2767 2415 2042 1808 713 648 359 159
13
MspA1I CmG'CkG
Cuts at: 1606 4327 4513 4798 5287 8340 9017 10165 10410
Size: 2721 186 285 489 3053 677 1148 245
Cuts at: 10410 1606
Size: 2120
Fragments arranged by size:
3053 2721 2120 1148 677 489 285 245
186
NcoI C'CATG_G
Cuts at: 513 1065 2004 4578 4683 513
Size: 552 939 2574 105 6754
Fragments arranged by size:
6754 2574 939 552 105
NdeI CA'TA_TG
Cuts at: 387 3817 7888 387
Size: 3430 4071 3423
Fragments arranged by size:
4071 3430 3423
NheI G'CTAG_C
Cuts at: 1052 5087 7919 1052
Size: 4035 2832 4057
Fragments arranged by size:
4057 4035 2832
NotI GC'GGCC_GC
Cuts at: 8371 8371
Size: 10924
NsiI A_TGCA'T
Cuts at: 2940 5452 7266 8130 2940
Size: 2512 1814 864 5734
Fragments arranged by size:
5734 2512 1814 864
NspI r_CATG'y
Cuts at: 1132 1229 2938 4487 4577 5450 6371 6439 7963
Size: 97 1709 1549 90 873 921 68 1524
Cuts at: 7963 7986 8159 8282 9827 1132
Size: 23 173 123 1545 2229
Fragments arranged by size:
2229 1709 1549 1545 1524 921 873 173
123 97 90 68 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10924
PciI A'CATG_T
Cuts at: 6367 6435 7959 8155 9823 6367
Size: 68 1524 196 1668 7468
Fragments arranged by size:
7468 1668 1524 196 68
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2758 1108
Size: 1650 9274
PfoI T'CCnGG_A
Cuts at: 4014 4014
Size: 10924
PmeI GTTT'AAAC
Cuts at: 8228 8228
Size: 10924
PmlI CAC'GTG
Cuts at: 2344 2344
Size: 10924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7353 7385 8993 9025 10506 10538 7353
Size: 32 1608 32 1481 32 7739
Fragments arranged by size:
7739 1608 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10924
PsiI TTA'TAA
Cuts at: 5426 8494 5426
Size: 3068 7856
PspOMI G'GGCC_C
Cuts at: 2289 2289
Size: 10924
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3765 3797 3765
Size: 32 10892
PstI C_TGCA'G
Cuts at: 830 3744 4072 4852 5286 7786 8276 830
Size: 2914 328 780 434 2500 490 3478
Fragments arranged by size:
3478 2914 2500 780 490 434 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10924
PvuII CAG'CTG
Cuts at: 4327 4513 4798 4327
Size: 186 285 10453
Fragments arranged by size:
10453 285 186
SacI G_AGCT'C
Cuts at: 2064 4551 5934 6855 2064
Size: 2487 1383 921 6133
Fragments arranged by size:
6133 2487 1383 921
SalI G'TCGA_C
Cuts at: 1936 8266 1936
Size: 6330 4594
SapI GCTCTTCn'nnn_
Cuts at: 3301 3340 5388 6075 3301
Size: 39 2048 687 8150
Fragments arranged by size:
8150 2048 687 39
SbfI CC_TGCA'GG
Cuts at: 4072 8276 4072
Size: 4204 6720
ScaI AGT'ACT
Cuts at: 4883 6228 9172 10884 4883
Size: 1345 2944 1712 4923
Fragments arranged by size:
4923 2944 1712 1345
SfcI C'TryA_G
Cuts at: 826 1045 2326 3458 3740 4068 4848 5077 5282
Size: 219 1281 1132 282 328 780 229 205
Cuts at: 5282 6581 7782 8272 9407 10088 10279 826
Size: 1299 1201 490 1135 681 191 1471
Fragments arranged by size:
1471 1299 1281 1201 1135 1132 780 681
490 328 282 229 219 205 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10924
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10924
SmaI CCC'GGG
Cuts at: 1872 8251 1872
Size: 6379 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3618 3698 4473 7212 7393
Size: 197 926 786 889 80 775 2739 181
Cuts at: 7393 9028 9929 10191 820
Size: 1635 901 262 1553
Fragments arranged by size:
2739 1635 1553 926 901 889 786 775
262 197 181 80
SnaBI TAC'GTA
Cuts at: 493 8715 493
Size: 8222 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10924
SphI G_CATG'C
Cuts at: 1132 2938 4577 7986 8282 1132
Size: 1806 1639 3409 296 3774
Fragments arranged by size:
3774 3409 1806 1639 296
SspI AAT'ATT
Cuts at: 5 52 3856 3899 4559 4836 8848 5
Size: 47 3804 43 660 277 4012 2081
Fragments arranged by size:
4012 3804 2081 660 277 47 43
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10924
StyI C'CwwG_G
Cuts at: 513 1065 2004 2893 3562 3981 4578 4683 8363
Size: 552 939 889 669 419 597 105 3680
Cuts at: 8363 513
Size: 3074
Fragments arranged by size:
3680 3074 939 889 669 597 552 419
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3187 6060 7574 8205 8951 8968 9121
Size: 1255 1607 2873 1514 631 746 17 153
Cuts at: 9121 9306 9751 325
Size: 185 445 1498
Fragments arranged by size:
2873 1607 1514 1498 1255 746 631 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4881 6226 7347
Size: 274 80 33 51 1443 2904 1345 1121
Cuts at: 7347 9170 10882 96
Size: 1823 1712 138
Fragments arranged by size:
2904 1823 1712 1443 1345 1121 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10884 10916 10884
Size: 32 10892
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10924
XbaI T'CTAG_A
Cuts at: 8260 8260
Size: 10924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4930 4930
Size: 10924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10924
XmaI C'CCGG_G
Cuts at: 1870 8249 1870
Size: 6379 4545
XmnI GAAnn'nnTTC
Cuts at: 2418 2564 3429 3693 3780 9053 2418
Size: 146 865 264 87 5273 4289
Fragments arranged by size:
5273 4289 865 264 146 87
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8732 276
Size: 53 83 186 936 7198 2468
Fragments arranged by size:
7198 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BsiWI BstXI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI