Restriction map for
pFN21AE2359
(Circular) (Six-base) MAPSORT of: pFN21AE2359.seq Check: 3367 from: 1 to: 5866
pFN21AE2359 5866 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2597 2869 2597
Size: 272 5594
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2307 3676 278
Size: 53 83 186 936 771 1369 2468
Fragments arranged by size:
2468 1369 936 771 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3209 1937
Size: 1272 4594
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3929 5313 5581 1632
Size: 2297 1384 268 1917
Fragments arranged by size:
2297 1917 1384 268
AfeI AGC'GCT
Cuts at: 3002 3002
Size: 5866
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 3070 4765 5742 1203
Size: 1867 1695 977 1327
Fragments arranged by size:
1867 1695 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 3609 5181 3609
Size: 1572 4294
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 3175 3401 3553 5662 1493
Size: 1682 226 152 2109 1697
Fragments arranged by size:
2109 1697 1682 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2423 3057 4420 160
Size: 872 1391 634 1363 1606
Fragments arranged by size:
1606 1391 1363 872 634
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 3191 1870
Size: 73 1173 75 4545
Fragments arranged by size:
4545 1173 75 73
BamHI G'GATC_C
Cuts at: 2848 3062 3196 3563 2848
Size: 214 134 367 5151
Fragments arranged by size:
5151 367 214 134
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2821 3187 4642 618
Size: 325 217 627 447 587 366 1455 1842
Fragments arranged by size:
1842 1455 627 587 447 366 325 217
BanII G_rGCy'C
Cuts at: 1391 3185 1391
Size: 1794 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2551 3016 928
Size: 971 652 465 3778
Fragments arranged by size:
3778 971 652 465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2824 3927 5083 1790
Size: 1034 1103 1156 2573
Fragments arranged by size:
2573 1156 1103 1034
BmgBI CAC'GTC
Cuts at: 1318 2966 1318
Size: 1648 4218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2739
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4526 1239
Size: 3287 2579
Bpu10I CC'TnA_GC
Cuts at: 2153 2661 2153
Size: 508 5358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2759 4529 882
Size: 617 1260 1770 2219
Fragments arranged by size:
2219 1770 1260 617
BsaAI yAC'GTr
Cuts at: 493 1204 2550 3657 493
Size: 711 1346 1107 2702
Fragments arranged by size:
2702 1346 1107 711
BsaBI GATnn'nnATC
Cuts at: 2102 5860 2102
Size: 3758 2108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 3673 4055 4690
Size: 53 83 186 936 771 1369 382 635
Cuts at: 4690 275
Size: 1451
Fragments arranged by size:
1451 1369 936 771 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2612 2698 1186
Size: 865 561 86 4354
Fragments arranged by size:
4354 865 561 86
BseYI C'CCAG_C
Cuts at: 2139 2428 2439 2817 5069 5565 2139
Size: 289 11 378 2252 496 2440
Fragments arranged by size:
2440 2252 496 378 289 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3077 1163
Size: 573 1341 3952
Fragments arranged by size:
3952 1341 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3927 4012 5083 3927
Size: 85 1071 4710
Fragments arranged by size:
4710 1071 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2824 3185 3927 4012 5083 1391
Size: 399 1034 361 742 85 1071 2174
Fragments arranged by size:
2174 1071 1034 742 399 361 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2597 2869 3221 844
Size: 1280 473 272 352 3489
Fragments arranged by size:
3489 1280 473 352 272
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2845 2245
Size: 600 5266
BstYI r'GATC_y
Cuts at: 1972 2200 2679 2848 3062 3196 3563 3948 3965
Size: 228 479 169 214 134 367 385 17
Cuts at: 3965 5417 5503 5515 5861 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 228 214
169 134 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2401 2735 487
Size: 683 56 1175 334 3618
Fragments arranged by size:
3618 1175 683 334 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 2324 1554
Size: 770 5096
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2867 4873 809
Size: 507 1551 2006 1802
Fragments arranged by size:
2006 1802 1551 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3313 4202
Size: 54 1425 50 275 18 270 1213 889
Cuts at: 4202 5831 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1213 889 275 270 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3796 1353
Size: 2443 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3929 4526 5313 5581 1239
Size: 393 2297 597 787 268 1524
Fragments arranged by size:
2297 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 2414 4372 2414
Size: 1958 3908
HaeII r_GCGC'y
Cuts at: 1678 3004 5013 1678
Size: 1326 2009 2531
Fragments arranged by size:
2531 2009 1326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2392 2424 3131 3163 4507
Size: 32 135 32 820 32 707 32 1344
Cuts at: 4507 4539 4581 4613 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 820 707 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2569 3210 3456 669
Size: 988 281 631 641 246 3079
Fragments arranged by size:
3079 988 641 631 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4980 5164 1305
Size: 3675 184 2007
Fragments arranged by size:
3675 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 10
Size: 54 1425 343 270 3774
Fragments arranged by size:
3774 1425 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2795 3824 4183 4342 518
Size: 648 1629 1029 359 159 2042
Fragments arranged by size:
2042 1629 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3282 3959 5107 5352 1606
Size: 1676 677 1148 245 2120
Fragments arranged by size:
2120 1676 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4375
Fragments arranged by size:
4375 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2672 3074 3224 4769 1132
Size: 97 1443 402 150 1545 2229
Fragments arranged by size:
2229 1545 1443 402 150 97
PasI CC'CwG_GG
Cuts at: 1348 2873 1348
Size: 1525 4341
PciI A'CATG_T
Cuts at: 3070 4765 3070
Size: 1695 4171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 1108
Size: 1275 4591
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 3935 3967 5448 5480 2528
Size: 32 1375 32 1481 32 2914
Fragments arranged by size:
2914 1481 1375 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SfcI C'TryA_G
Cuts at: 826 1045 2389 3214 4349 5030 5221 826
Size: 219 1344 825 1135 681 191 1471
Fragments arranged by size:
1471 1344 1135 825 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3116 3970 4871 5133 820
Size: 197 926 1173 854 901 262 1553
Fragments arranged by size:
1553 1173 926 901 854 262 197
SnaBI TAC'GTA
Cuts at: 493 2550 3657 493
Size: 2057 1107 2702
Fragments arranged by size:
2702 2057 1107
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 2672 3224 1132
Size: 1540 552 3774
Fragments arranged by size:
3774 1540 552
SspI AAT'ATT
Cuts at: 5 52 2842 3790 5
Size: 47 2790 948 2081
Fragments arranged by size:
2790 2081 948 47
StuI AGG'CCT
Cuts at: 1517 2722 1517
Size: 1205 4661
StyI C'CwwG_G
Cuts at: 513 1065 2004 3305 513
Size: 552 939 1301 3074
Fragments arranged by size:
3074 1301 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 3910 4063 4248 4693 325
Size: 1255 2313 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3073 4112 5824
Size: 274 80 33 51 1443 1096 1039 1712
Cuts at: 5824 96
Size: 138
Fragments arranged by size:
1712 1443 1096 1039 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 3116 1943
Size: 1173 4693
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3013 3995 3013
Size: 982 4884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2305 3674 276
Size: 53 83 186 936 771 1369 2468
Fragments arranged by size:
2468 1369 936 771 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI