Restriction map for
pFN21AE2358
(Circular) (Six-base) MAPSORT of: pFN21AE2358.seq Check: 9040 from: 1 to: 5968
pFN21AE2358 5968 bp
With 182 enzymes: SgfI *
July 7, 2011 17:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2759 4031 5415 5683 1632
Size: 1127 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 1127 268
AfeI AGC'GCT
Cuts at: 2384 2384
Size: 5968
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 2490 4867 5844 1203
Size: 1287 2377 977 1327
Fragments arranged by size:
2377 1327 1287 977
AgeI A'CCGG_T
Cuts at: 2056 4799 2056
Size: 2743 3225
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 3234 1166
Size: 2068 3900
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 2465 3711 5283 2465
Size: 1246 1572 3150
Fragments arranged by size:
3150 1572 1246
ApaI G_GGCC'C
Cuts at: 2472 2472
Size: 5968
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2694 3277 3503 3655 5764 1493
Size: 1201 583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1201 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2261 2351 2399 3293 1870
Size: 73 318 90 48 894 4545
Fragments arranged by size:
4545 894 318 90 73 48
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3289 4744 618
Size: 325 217 627 1502 1455 1842
Fragments arranged by size:
1842 1502 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2235 2262 2472 2715 3168 3175 3287
Size: 640 204 27 210 243 453 7 112
Cuts at: 3287 1391
Size: 4072
Fragments arranged by size:
4072 640 453 243 210 204 112 27
7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2592 928
Size: 971 693 4304
Fragments arranged by size:
4304 971 693
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2239 4579
Size: 107 122 71 631 605 337 230 2340
Cuts at: 4579 136
Size: 1525
Fragments arranged by size:
2340 1525 631 605 337 230 122 107
71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 2472 3084 4029 5185 1790
Size: 682 612 945 1156 2573
Fragments arranged by size:
2573 1156 945 682 612
BmgBI CAC'GTC
Cuts at: 1318 2296 1318
Size: 978 4990
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3699 4657 442
Size: 416 53 83 399 24 2282 958 1753
Fragments arranged by size:
2282 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2462 2462
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2210 2342 2523 2537 2639 3194 4628
Size: 806 165 132 181 14 102 555 1434
Cuts at: 4628 1239
Size: 2579
Fragments arranged by size:
2579 1434 806 555 181 165 132 102
14
Bpu10I CC'TnA_GC
Cuts at: 3186 3186
Size: 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2249 4093 4958 5256 2249
Size: 1844 865 298 2961
Fragments arranged by size:
2961 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2807 4631 882
Size: 617 1308 1824 2219
Fragments arranged by size:
2219 1824 1308 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2832 3775 4157 4792
Size: 53 83 186 936 1299 943 382 635
Cuts at: 4792 275
Size: 1451
Fragments arranged by size:
1451 1299 943 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2056 4401 4799 5073 5220 5631 2056
Size: 2345 398 274 147 411 2393
Fragments arranged by size:
2393 2345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3058 1186
Size: 1872 4096
BseYI C'CCAG_C
Cuts at: 2046 2206 2540 5171 5667 2046
Size: 160 334 2631 496 2347
Fragments arranged by size:
2631 2347 496 334 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 2954 4029 4114 5185 2715
Size: 239 1075 85 1071 3498
Fragments arranged by size:
3498 1075 1071 239 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2600
Size: 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2235 2262 2472 2715 2954 3084
Size: 399 241 204 27 210 243 239 130
Cuts at: 3084 3168 3175 3287 4029 4114 5185 1391
Size: 84 7 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 243 241 239 210
204 130 112 85 84 27 7
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3323 844
Size: 2479 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4463 4637 65
Size: 1183 3215 174 1396
Fragments arranged by size:
3215 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2095 2416 4612 4799 1700
Size: 110 246 39 321 2196 187 2869
Fragments arranged by size:
2869 2196 321 246 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 2107 2133 2153 2194 2196 2422 1925
Size: 182 26 20 41 2 226 5471
Fragments arranged by size:
5471 226 182 41 26 20 2
BssSI C'ACGA_G
Cuts at: 2378 3242 4028 5040 2378
Size: 864 786 1012 3306
Fragments arranged by size:
3306 1012 864 786
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 2854 1184
Size: 1670 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 2602 2602
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963
Size: 1326 367 385 17 1452 86 12 346
Cuts at: 5963 1972
Size: 1977
Fragments arranged by size:
1977 1452 1326 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2661 2661
Size: 5968
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2235 2313 2601 2724 3037
Size: 552 939 27 204 78 288 123 313
Cuts at: 3037 3180 513
Size: 143 3301
Fragments arranged by size:
3301 939 552 313 288 204 143 123
78 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2335 2778
Size: 54 1425 50 275 18 480 25 443
Cuts at: 2778 3238 3415 4304 5933 8
Size: 460 177 889 1629 43
Fragments arranged by size:
1629 1425 889 480 460 443 275 177
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 2915 2987 3898 1353
Size: 1100 462 72 911 3423
Fragments arranged by size:
3423 1100 911 462 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2713 2713
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2210 2342 2523 2537 2639 2759
Size: 393 413 165 132 181 14 102 120
Cuts at: 2759 3194 4031 4628 5415 5683 1239
Size: 435 837 597 787 268 1524
Fragments arranged by size:
1524 837 787 597 435 413 393 268
181 165 132 120 102 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2904 2904
Size: 5968
EcoO109I rG'GnC_Cy
Cuts at: 2469 2909 2469
Size: 440 5528
EcoRI G'AATT_C
Cuts at: 3277 3655 3277
Size: 378 5590
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 2386 5115 1678
Size: 708 2729 2531
Fragments arranged by size:
2729 2531 708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 4609 4641 4683
Size: 32 135 32 1027 32 1978 32 42
Cuts at: 4683 4715 1373
Size: 32 2626
Fragments arranged by size:
2626 1978 1027 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2836 3312 3558 669
Size: 988 281 898 476 246 3079
Fragments arranged by size:
3079 988 898 476 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5082 5266 1305
Size: 3777 184 2007
Fragments arranged by size:
3777 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2337 2780 3240 10
Size: 54 1425 343 505 443 460 2738
Fragments arranged by size:
2738 1425 505 460 443 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2852 2933 3234 3926 4285 4444 518
Size: 648 1686 81 301 692 359 159 2042
Fragments arranged by size:
2042 1686 692 648 359 301 159 81
MspA1I CmG'CkG
Cuts at: 1606 2033 2040 3384 4061 5209 5454 1606
Size: 427 7 1344 677 1148 245 2120
Fragments arranged by size:
2120 1344 1148 677 427 245 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2235 2601 2724 3037 3180 513
Size: 552 939 231 366 123 313 143 3301
Fragments arranged by size:
3301 939 552 366 313 231 143 123
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 2494 3131 3326 4871 1132
Size: 97 1265 637 195 1545 2229
Fragments arranged by size:
2229 1545 1265 637 195 97
PasI CC'CwG_GG
Cuts at: 1348 3168 1348
Size: 1820 4148
PciI A'CATG_T
Cuts at: 2490 4867 2490
Size: 2377 3591
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2465 2681 1108
Size: 1357 216 4395
Fragments arranged by size:
4395 1357 216
PfoI T'CCnGG_A
Cuts at: 2022 2674 2022
Size: 652 5316
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2909 2909
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 2468 2468
Size: 5968
PstI C_TGCA'G
Cuts at: 830 3320 830
Size: 2490 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
SacI G_AGCT'C
Cuts at: 2715 2715
Size: 5968
SacII CC_GC'GG
Cuts at: 2034 2034
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 2915 2453
Size: 462 5506
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 2624 4216 5928 2624
Size: 1592 1712 2664
Fragments arranged by size:
2664 1712 1592
SfcI C'TryA_G
Cuts at: 826 1045 3316 4451 5132 5323 826
Size: 219 2271 1135 681 191 1471
Fragments arranged by size:
2271 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2353 2401 3295 1872
Size: 481 48 894 4545
Fragments arranged by size:
4545 894 481 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2228 4072 4973 5235 820
Size: 197 926 285 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 285 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3131 3326 1132
Size: 1999 195 3774
Fragments arranged by size:
3774 1999 195
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 3017 1517
Size: 1500 4468
StyI C'CwwG_G
Cuts at: 513 1065 2004 2173 2235 2253 2271 2307 2601
Size: 552 939 169 62 18 18 36 294
Cuts at: 2601 2724 3037 3132 3180 3407 513
Size: 123 313 95 48 227 3074
Fragments arranged by size:
3074 939 552 313 294 227 169 123
95 62 48 36 18 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3224 3995 4012 4165 4350 4795 325
Size: 1255 1644 771 17 153 185 445 1498
Fragments arranged by size:
1644 1498 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 4214 5926
Size: 274 80 33 51 1443 645 1592 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1712 1592 1443 645 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3187 3187
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 2351 2399 3293 1870
Size: 481 48 894 4545
Fragments arranged by size:
4545 894 481 48
XmnI GAAnn'nnTTC
Cuts at: 2924 4097 2924
Size: 1173 4795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI
SwaI