Restriction map for
pFN21AE2357
(Circular) (Six-base) MAPSORT of: pFN21AE2357.seq Check: 6464 from: 1 to: 11650
pFN21AE2357 11650 bp
With 182 enzymes: SgfI *
July 22, 2011 10:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6746 6746
Size: 11650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9460 278
Size: 53 83 186 936 7924 2468
Fragments arranged by size:
7924 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3222 4236 4260 8993 1937
Size: 1285 1014 24 4733 4594
Fragments arranged by size:
4733 4594 1285 1014 24
Acc65I G'GTAC_C
Cuts at: 5851 6665 8971 5851
Size: 814 2306 8530
Fragments arranged by size:
8530 2306 814
AclI AA'CG_TT
Cuts at: 1563 9777 10150 1563
Size: 8214 373 3063
Fragments arranged by size:
8214 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3146 3297 3591 3747 3774 4468 4527 4647
Size: 1514 151 294 156 27 694 59 120
Cuts at: 4647 4752 5559 6468 7179 7449 8484 9713 11097
Size: 105 807 909 711 270 1035 1229 1384
Cuts at: 11097 11365 1632
Size: 268 1917
Fragments arranged by size:
1917 1514 1384 1229 1035 909 807 711
694 294 270 268 156 151 120 105
59 27
AflII C'TTAA_G
Cuts at: 820 1017 2234 820
Size: 197 1217 10236
Fragments arranged by size:
10236 1217 197
AflIII A'CryG_T
Cuts at: 1203 2168 4048 5223 7669 10549 11526 1203
Size: 965 1880 1175 2446 2880 977 1327
Fragments arranged by size:
2880 2446 1880 1327 1175 977 965
AgeI A'CCGG_T
Cuts at: 4255 10481 4255
Size: 6226 5424
AhdI GACnn_n'nnGTC
Cuts at: 3011 7673 10379 3011
Size: 4662 2706 4282
Fragments arranged by size:
4662 4282 2706
AleI CACnn'nnGTG
Cuts at: 1166 7803 1166
Size: 6637 5013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5231 5955 7605 1184
Size: 4047 724 1650 5229
Fragments arranged by size:
5229 4047 1650 724
AlwNI CAG_nnn'CTG
Cuts at: 3881 4592 4775 8092 9393 10965 3881
Size: 711 183 3317 1301 1572 4566
Fragments arranged by size:
4566 3317 1572 1301 711 183
ApaLI G'TGCA_C
Cuts at: 4452 9707 10863 4452
Size: 5255 1156 5239
Fragments arranged by size:
5255 5239 1156
ApoI r'AATT_y
Cuts at: 1493 2246 2447 2572 3089 3467 3505 4676 5015
Size: 753 201 125 517 378 38 1171 339
Cuts at: 5015 5081 5169 5686 6009 6442 7163 7609 8276
Size: 66 88 517 323 433 721 446 667
Cuts at: 8276 8396 8743 8959 9185 9337 11446 1493
Size: 120 347 216 226 152 2109 1697
Fragments arranged by size:
2109 1697 1171 753 721 667 517 517
446 433 378 347 339 323 226 216
201 152 125 120 88 66 38
AseI AT'TA_AT
Cuts at: 160 1032 2316 3266 10204 160
Size: 872 1284 950 6938 1606
Fragments arranged by size:
6938 1606 1284 950 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11650
AvaI C'yCGr_G
Cuts at: 1870 1943 3009 8975 1870
Size: 73 1066 5966 4545
Fragments arranged by size:
5966 4545 1066 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5709 5742 6069 6102 6456 6489 7956 7989 5709
Size: 33 327 33 354 33 1467 33 9370
Fragments arranged by size:
9370 1467 354 327 33 33 33 33
BamHI G'GATC_C
Cuts at: 3937 6744 8980 9347 3937
Size: 2807 2236 367 6240
Fragments arranged by size:
6240 2807 2236 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4564 5851 6665 8971 10426
Size: 325 217 627 2777 1287 814 2306 1455
Cuts at: 10426 618
Size: 1842
Fragments arranged by size:
2777 2306 1842 1455 1287 814 627 325
217
BanII G_rGCy'C
Cuts at: 1391 4617 4811 7646 8969 1391
Size: 3226 194 2835 1323 4072
Fragments arranged by size:
4072 3226 2835 1323 194
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4073 4431 4917 7663 7945 928
Size: 971 2174 358 486 2746 282 4633
Fragments arranged by size:
4633 2746 2174 971 486 358 282
BbvCI CC'TCA_GC
Cuts at: 5521 6888 8843 5521
Size: 1367 1955 8328
Fragments arranged by size:
8328 1955 1367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7247 7281 9841 9875 1903
Size: 34 50 34 5226 34 2560 34 3678
Fragments arranged by size:
5226 3678 2560 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2069 6484 9542 10752 2069
Size: 4415 3058 1210 2967
Fragments arranged by size:
4415 3058 2967 1210
BclI T'GATC_A
Cuts at: 1548 5338 6094 7291 7516 8775 1548
Size: 3790 756 1197 225 1259 4423
Fragments arranged by size:
4423 3790 1259 1197 756 225
BfrBI ATG'CAT
Cuts at: 4756 4756
Size: 11650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10261 136
Size: 107 122 71 631 605 8589 1525
Fragments arranged by size:
8589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2209 3644 4123 11645 2209
Size: 1435 479 7522 2214
Fragments arranged by size:
7522 2214 1435 479
BlpI GC'TnA_GC
Cuts at: 9067 9067
Size: 11650
Bme1580I G_kGCm'C
Cuts at: 1790 4456 4569 5613 9711 10867 1790
Size: 2666 113 1044 4098 1156 2573
Fragments arranged by size:
4098 2666 2573 1156 1044 113
BmgBI CAC'GTC
Cuts at: 1318 7670 1318
Size: 6352 5298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 2401 4088
Size: 416 53 83 399 24 890 94 1687
Cuts at: 4088 4401 5607 7656 9381 10339 442
Size: 313 1206 2049 1725 958 1753
Fragments arranged by size:
2049 1753 1725 1687 1206 958 890 416
399 313 94 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 7313 1056
Size: 6257 5393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3754 3768 4010 4183 5016 7009 7459 7830
Size: 2515 14 242 173 833 1993 450 371
Cuts at: 7830 8344 10310 1239
Size: 514 1966 2579
Fragments arranged by size:
2579 2515 1993 1966 833 514 450 371
242 173 14
Bpu10I CC'TnA_GC
Cuts at: 5521 6888 8093 8843 5521
Size: 1367 1205 750 8328
Fragments arranged by size:
8328 1367 1205 750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 4707 5385 9775 10640 10938 2477
Size: 2230 678 4390 865 298 3189
Fragments arranged by size:
4390 3189 2230 865 678 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4222 5510 10313 882
Size: 617 2723 1288 4803 2219
Fragments arranged by size:
4803 2723 2219 1288 617
BsaAI yAC'GTr
Cuts at: 493 1204 4049 9441 493
Size: 711 2845 5392 2702
Fragments arranged by size:
5392 2845 2702 711
BsaBI GATnn'nnATC
Cuts at: 2208 2465 3105 11644 2208
Size: 257 640 8539 2214
Fragments arranged by size:
8539 2214 640 257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9457 9839 10474 275
Size: 53 83 186 936 7924 382 635 1451
Fragments arranged by size:
7924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4255 10083 10481 10755 10902 11313 4255
Size: 5828 398 274 147 411 4592
Fragments arranged by size:
5828 4592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2701 2731 7498 7528 1178
Size: 30 165 30 1298 30 4767 30 5300
Fragments arranged by size:
5300 4767 1298 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2361 2706 3308 3725 3728 7559 7955
Size: 847 328 345 602 417 3 3831 396
Cuts at: 7955 8030 8697 1186
Size: 75 667 4139
Fragments arranged by size:
4139 3831 847 667 602 417 396 345
328 75 3
BseYI C'CCAG_C
Cuts at: 4592 4616 4736 8289 10853 11349 4592
Size: 24 120 3553 2564 496 4893
Fragments arranged by size:
4893 3553 2564 496 120 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2707 5696 6993 7217 8922 1163
Size: 573 971 2989 1297 224 1705 3891
Fragments arranged by size:
3891 2989 1705 1297 971 573 224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9100 9861 10889 1540
Size: 275 186 7099 761 1028 2301
Fragments arranged by size:
7099 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2134 4456 6592 8710 8858 9711 9796 10867 2134
Size: 2322 2136 2118 148 853 85 1071 2917
Fragments arranged by size:
2917 2322 2136 2118 1071 853 148 85
BsmI GAATG_Cn'
Cuts at: 2034 5254 9161 9254 2034
Size: 3220 3907 93 4430
Fragments arranged by size:
4430 3907 3220 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4661 4661
Size: 11650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 4456 4569 4617 4811 5613 6592
Size: 399 344 2322 113 48 194 802 979
Cuts at: 6592 7646 8710 8858 8969 9711 9796 10867 1391
Size: 1054 1064 148 111 742 85 1071 2174
Fragments arranged by size:
2322 2174 1071 1064 1054 979 802 742
399 344 194 148 113 111 85 48
BspEI T'CCGG_A
Cuts at: 11313 11313
Size: 11650
BspHI T'CATG_A
Cuts at: 2923 5341 7476 7960 8668 9539 11269 2923
Size: 2418 2135 484 708 871 1730 3304
Fragments arranged by size:
3304 2418 2135 1730 871 708 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6746 9005 844
Size: 5902 2259 3489
Fragments arranged by size:
5902 3489 2259
BsrBI CCG'CTC
Cuts at: 1394 1823 9537 1394
Size: 429 7714 3507
Fragments arranged by size:
7714 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 8147 8278 8906 10145 10319 65
Size: 1183 6899 131 628 1239 174 1396
Fragments arranged by size:
6899 1396 1239 1183 628 174 131
BsrFI r'CCGG_y
Cuts at: 1700 1810 4255 6233 10294 10481 1700
Size: 110 2445 1978 4061 187 2869
Fragments arranged by size:
4061 2869 2445 1978 187 110
BsrGI T'GTAC_A
Cuts at: 96 5862 96
Size: 5766 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11650
BssSI C'ACGA_G
Cuts at: 7490 9710 10722 7490
Size: 2220 1012 8418
Fragments arranged by size:
8418 2220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3785 7319 7763 794
Size: 2991 3534 444 4681
Fragments arranged by size:
4681 3534 2991 444
BstBI TT'CG_AA
Cuts at: 10454 10454
Size: 11650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11650
BstXI CCAn_nnnn'nTGG
Cuts at: 6155 8887 6155
Size: 2732 8918
BstYI r'GATC_y
Cuts at: 1972 2209 2641 3644 3937 4123 4341 6744 8980
Size: 237 432 1003 293 186 218 2403 2236
Cuts at: 8980 9347 9732 9749 11201 11287 11299 11645 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2403 2236 1977 1452 1003 432 385 367
346 293 237 218 186 86 17 12
BstZ17I GTA'TAC
Cuts at: 4237 4261 4237
Size: 24 11626
BtgI C'CryG_G
Cuts at: 513 1065 4221 8748 513
Size: 552 3156 4527 3415
Fragments arranged by size:
4527 3415 3156 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10911
Fragments arranged by size:
10911 683 56
BtsI GCAGTG_nn'
Cuts at: 4747 7703 7785 7798 7810 9166 9960 9980 4747
Size: 2956 82 13 12 1356 794 20 6417
Fragments arranged by size:
6417 2956 1356 794 82 20 13 12
ClaI AT'CG_AT
Cuts at: 1554 7267 1554
Size: 5713 5937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7473 7508 529
Size: 35 6909 35 4671
Fragments arranged by size:
6909 4671 35 35
DraI TTT'AAA
Cuts at: 2368 2752 3504 3618 8954 2368
Size: 384 752 114 5336 5064
Fragments arranged by size:
5336 5064 752 384 114
DraIII CAC_nnn'GTG
Cuts at: 3595 5848 7698 3595
Size: 2253 1850 7547
Fragments arranged by size:
7547 2253 1850
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 4075 10657 809
Size: 507 1020 1739 6582 1802
Fragments arranged by size:
6582 1802 1739 1020 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5889 8374 8408
Size: 54 1425 50 275 18 4059 2485 34
Cuts at: 8408 8576 9097 9986 11615 8
Size: 168 521 889 1629 43
Fragments arranged by size:
4059 2485 1629 1425 889 521 275 168
54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 9097 1537
Size: 275 7285 4090
Fragments arranged by size:
7285 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2910 3294 4284 5008 7568 8862 9580
Size: 747 810 384 990 724 2560 1294 718
Cuts at: 9580 1353
Size: 3423
Fragments arranged by size:
3423 2560 1294 990 810 747 724 718
384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10237 10611 10757 387
Size: 1064 138 8648 374 146 1280
Fragments arranged by size:
8648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3146 3297 3591 3747 3754 3768 3774
Size: 393 1514 151 294 156 7 14 6
Cuts at: 3774 4010 4183 4468 4527 4647 4752 5016 5559
Size: 236 173 285 59 120 105 264 543
Cuts at: 5559 6468 7009 7179 7449 7459 7830 8344 8484
Size: 909 541 170 270 10 371 514 140
Cuts at: 8484 9713 10310 11097 11365 1239
Size: 1229 597 787 268 1524
Fragments arranged by size:
1524 1514 1229 909 787 597 543 541
514 393 371 294 285 270 268 264
236 173 170 156 151 140 120 105
59 14 10 7 6
EcoO109I rG'GnC_Cy
Cuts at: 4109 4772 5307 7784 4109
Size: 663 535 2477 7975
Fragments arranged by size:
7975 2477 663 535
EcoRI G'AATT_C
Cuts at: 3467 6442 8276 8959 9337 3467
Size: 2975 1834 683 378 5780
Fragments arranged by size:
5780 2975 1834 683 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4585 4585
Size: 11650
FspI TGC'GCA
Cuts at: 10156 10156
Size: 11650
HaeII r_GCGC'y
Cuts at: 1678 3327 5126 7774 10797 1678
Size: 1649 1799 2648 3023 2531
Fragments arranged by size:
3023 2648 2531 1799 1649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2701 2733 2997
Size: 32 135 32 680 32 417 32 264
Cuts at: 2997 3029 3797 3829 3989 4021 4202 4234 4628
Size: 32 768 32 160 32 181 32 394
Cuts at: 4628 4660 5441 5473 6299 6331 7019 7051 7659
Size: 32 781 32 826 32 688 32 608
Cuts at: 7659 7691 8764 8796 8861 8893 10291 10323 10365
Size: 32 1073 32 65 32 1398 32 42
Cuts at: 10365 10397 1373
Size: 32 2626
Fragments arranged by size:
2626 1398 1073 826 781 768 688 680
608 417 394 264 181 160 135 65
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 3223 4228 5578 7213 7882
Size: 988 281 799 486 1005 1350 1635 669
Cuts at: 7882 8994 9240 669
Size: 1112 246 3079
Fragments arranged by size:
3079 1635 1350 1112 1005 988 799 669
486 281 246
HindIII A'AGCT_T
Cuts at: 748 3737 5990 7389 748
Size: 2989 2253 1399 5009
Fragments arranged by size:
5009 2989 2253 1399
HpaI GTT'AAC
Cuts at: 9240 9240
Size: 11650
KpnI G_GTAC'C
Cuts at: 5855 6669 8975 5855
Size: 814 2306 8530
Fragments arranged by size:
8530 2306 814
MfeI C'AATT_G
Cuts at: 5958 9249 5958
Size: 3291 8359
MluI A'CGCG_T
Cuts at: 11526 11526
Size: 11650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2189 3042 3982 6086 6876 7072 7085 7263
Size: 884 853 940 2104 790 196 13 178
Cuts at: 7263 7795 10764 10948 1305
Size: 532 2969 184 2007
Fragments arranged by size:
2969 2104 2007 940 884 853 790 532
196 184 178 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 8376 8410 8578 10
Size: 54 1425 343 6544 34 168 3082
Fragments arranged by size:
6544 3082 1425 343 168 54 34
MslI CAynn'nnrTG
Cuts at: 518 1166 2834 5213 6003 6153 6552 7803 8019
Size: 648 1668 2379 790 150 399 1251 216
Cuts at: 8019 8025 8180 8456 9608 9967 10126 518
Size: 6 155 276 1152 359 159 2042
Fragments arranged by size:
2379 2042 1668 1251 1152 790 648 399
359 276 216 159 155 150 6
MspA1I CmG'CkG
Cuts at: 1606 2343 5606 6137 7427 9066 9743 10891 11136
Size: 737 3263 531 1290 1639 677 1148 245
Cuts at: 11136 1606
Size: 2120
Fragments arranged by size:
3263 2120 1639 1290 1148 737 677 531
245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 11098
NdeI CA'TA_TG
Cuts at: 387 3176 3654 3859 5757 387
Size: 2789 478 205 1898 6280
Fragments arranged by size:
6280 2789 1898 478 205
NheI G'CTAG_C
Cuts at: 1052 7309 1052
Size: 6257 5393
NotI GC'GGCC_GC
Cuts at: 9097 9097
Size: 11650
NsiI A_TGCA'T
Cuts at: 4758 4758
Size: 11650
NspI r_CATG'y
Cuts at: 1132 1229 2172 3229 4391 4577 4760 5227 5299
Size: 97 943 1057 1162 186 183 467 72
Cuts at: 5299 5710 5936 6125 7127 7142 9008 10553 1132
Size: 411 226 189 1002 15 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 1162 1057 1002 943 467
411 226 189 186 183 97 72 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11650
PciI A'CATG_T
Cuts at: 2168 5223 10549 2168
Size: 3055 5326 3269
Fragments arranged by size:
5326 3269 3055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3061 4592 5848 7780 8356 8383 1108
Size: 1953 1531 1256 1932 576 27 4375
Fragments arranged by size:
4375 1953 1932 1531 1256 576 27
PfoI T'CCnGG_A
Cuts at: 6775 8047 6775
Size: 1272 10378
PmeI GTTT'AAAC
Cuts at: 8954 8954
Size: 11650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9719 9751 11232 11264 9719
Size: 32 1481 32 10105
Fragments arranged by size:
10105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4109 5307 4109
Size: 1198 10452
PshAI GACnn'nnGTC
Cuts at: 1613 6504 1613
Size: 4891 6759
PsiI TTA'TAA
Cuts at: 4704 8501 8618 8627 9220 4704
Size: 3797 117 9 593 7134
Fragments arranged by size:
7134 3797 593 117 9
PspXI vC'TCGA_Gb
Cuts at: 3009 3009
Size: 11650
PstI C_TGCA'G
Cuts at: 830 3676 4090 4959 7805 8131 9002 830
Size: 2846 414 869 2846 326 871 3478
Fragments arranged by size:
3478 2846 2846 871 869 414 326
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11650
PvuII CAG'CTG
Cuts at: 5606 6137 7427 5606
Size: 531 1290 9829
Fragments arranged by size:
9829 1290 531
SalI G'TCGA_C
Cuts at: 1936 3221 8992 1936
Size: 1285 5771 4594
Fragments arranged by size:
5771 4594 1285
SanDI GG'GwC_CC
Cuts at: 4109 5307 4109
Size: 1198 10452
SbfI CC_TGCA'GG
Cuts at: 9002 9002
Size: 11650
ScaI AGT'ACT
Cuts at: 5536 7523 7724 9898 11610 5536
Size: 1987 201 2174 1712 5576
Fragments arranged by size:
5576 2174 1987 1712 201
SexAI A'CCwGG_T
Cuts at: 8760 8760
Size: 11650
SfcI C'TryA_G
Cuts at: 826 1045 2080 3672 3975 4086 4291 4955 5073
Size: 219 1035 1592 303 111 205 664 118
Cuts at: 5073 6108 6346 7801 8127 8998 10133 10814 11005
Size: 1035 238 1455 326 871 1135 681 191
Cuts at: 11005 826
Size: 1471
Fragments arranged by size:
1592 1471 1455 1135 1035 1035 871 681
664 326 303 238 219 205 191 118
111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11650
SmaI CCC'GGG
Cuts at: 1872 8977 1872
Size: 7105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2456 3009 4722 5400 9754
Size: 197 926 291 222 553 1713 678 4354
Cuts at: 9754 10655 10917 820
Size: 901 262 1553
Fragments arranged by size:
4354 1713 1553 926 901 678 553 291
262 222 197
SnaBI TAC'GTA
Cuts at: 493 9441 493
Size: 8948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11650
SphI G_CATG'C
Cuts at: 1132 4391 4577 4760 9008 1132
Size: 3259 186 183 4248 3774
Fragments arranged by size:
4248 3774 3259 186 183
SspI AAT'ATT
Cuts at: 5 52 2200 5625 9574 5
Size: 47 2148 3425 3949 2081
Fragments arranged by size:
3949 3425 2148 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11650
StyI C'CwwG_G
Cuts at: 513 1065 3817 5289 7405 9089 513
Size: 552 2752 1472 2116 1684 3074
Fragments arranged by size:
3074 2752 2116 1684 1472 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 3107 4750 4856 6233 6401 7260
Size: 1255 922 605 1643 106 1377 168 859
Cuts at: 7260 9677 9694 9847 10032 10477 325
Size: 2417 17 153 185 445 1498
Fragments arranged by size:
2417 1643 1498 1377 1255 922 859 605
445 185 168 153 106 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2871 5534 5862
Size: 274 80 33 51 1443 894 2663 328
Cuts at: 5862 7031 7205 7521 7722 8542 9896 11608 96
Size: 1169 174 316 201 820 1354 1712 138
Fragments arranged by size:
2663 1712 1443 1354 1169 894 820 328
316 274 201 174 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7498 7530 11610 11642 7498
Size: 32 4080 32 7506
Fragments arranged by size:
7506 4080 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11650
XbaI T'CTAG_A
Cuts at: 2882 8986 2882
Size: 6104 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 4228 7669 7805 3062
Size: 1166 3441 136 6907
Fragments arranged by size:
6907 3441 1166 136
XhoI C'TCGA_G
Cuts at: 1943 3009 1943
Size: 1066 10584
XmaI C'CCGG_G
Cuts at: 1870 8975 1870
Size: 7105 4545
XmnI GAAnn'nnTTC
Cuts at: 2919 2955 3892 4305 4497 6998 8081 8167 9779
Size: 36 937 413 192 2501 1083 86 1612
Cuts at: 9779 2919
Size: 4790
Fragments arranged by size:
4790 2501 1612 1083 937 413 192 86
36
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9458 276
Size: 53 83 186 936 7924 2468
Fragments arranged by size:
7924 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI