Restriction map for
pFN21AE2354
(Circular) (Six-base) MAPSORT of: pFN21AE2354.seq Check: 6580 from: 1 to: 14377
pFN21AE2354 14377 bp
With 182 enzymes: SgfI *
June 21, 2011 13:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6565 6946 7123 7297 6565
Size: 381 177 174 13645
Fragments arranged by size:
13645 381 177 174
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2499 12187 278
Size: 53 83 186 936 963 9688 2468
Fragments arranged by size:
9688 2468 963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3912 6532 10093 11720 1937
Size: 1975 2620 3561 1627 4594
Fragments arranged by size:
4594 3561 2620 1975 1627
Acc65I G'GTAC_C
Cuts at: 10363 11698 10363
Size: 1335 13042
AclI AA'CG_TT
Cuts at: 1563 12504 12877 1563
Size: 10941 373 3063
Fragments arranged by size:
10941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2786 3290 3734 4128 4608 5202 6564 6753
Size: 1154 504 444 394 480 594 1362 189
Cuts at: 6753 6930 7107 7443 7851 8309 8421 8535 8794
Size: 177 177 336 408 458 112 114 259
Cuts at: 8794 8856 9465 9753 9894 10374 10404 11275 11552
Size: 62 609 288 141 480 30 871 277
Cuts at: 11552 12440 13824 14092 1632
Size: 888 1384 268 1917
Fragments arranged by size:
1917 1384 1362 1154 888 871 609 594
504 480 480 458 444 408 394 336
288 277 268 259 189 177 177 141
114 112 62 30
AflII C'TTAA_G
Cuts at: 820 1017 2636 3085 820
Size: 197 1619 449 12112
Fragments arranged by size:
12112 1619 449 197
AflIII A'CryG_T
Cuts at: 1203 3072 6618 11155 13276 14253 1203
Size: 1869 3546 4537 2121 977 1327
Fragments arranged by size:
4537 3546 2121 1869 1327 977
AgeI A'CCGG_T
Cuts at: 13208 13208
Size: 14377
AhdI GACnn_n'nnGTC
Cuts at: 13106 13106
Size: 14377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 14377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4764 1184
Size: 3580 10797
AlwNI CAG_nnn'CTG
Cuts at: 3638 4559 6746 7925 9563 9626 12120 13692 3638
Size: 921 2187 1179 1638 63 2494 1572 4323
Fragments arranged by size:
4323 2494 2187 1638 1572 1179 921 63
ApaI G_GGCC'C
Cuts at: 4706 8664 4706
Size: 3958 10419
ApaLI G'TGCA_C
Cuts at: 12434 13590 12434
Size: 1156 13221
ApoI r'AATT_y
Cuts at: 1493 2614 3337 4426 4860 5003 5099 5110 5328
Size: 1121 723 1089 434 143 96 11 218
Cuts at: 5328 5368 5582 5691 6031 6038 6377 7583 7606
Size: 40 214 109 340 7 339 1206 23
Cuts at: 7606 7646 7700 8187 8378 8405 8417 9306 10609
Size: 40 54 487 191 27 12 889 1303
Cuts at: 10609 10797 11131 11332 11429 11443 11686 11912 12064
Size: 188 334 201 97 14 243 226 152
Cuts at: 12064 14173 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1303 1206 1121 1089 889 723
487 434 340 339 334 243 226 218
214 201 191 188 152 143 109 97
96 54 40 40 27 23 14 12
11 7
AseI AT'TA_AT
Cuts at: 160 1032 2163 3002 12931 160
Size: 872 1131 839 9929 1606
Fragments arranged by size:
9929 1606 1131 872 839
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14377
AvaI C'yCGr_G
Cuts at: 1870 1943 11702 1870
Size: 73 9759 4545
Fragments arranged by size:
9759 4545 73
BamHI G'GATC_C
Cuts at: 2179 10914 11707 12074 2179
Size: 8735 793 367 4482
Fragments arranged by size:
8735 4482 793 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 8618 10363 11698 13153 618
Size: 325 217 627 6831 1745 1335 1455 1842
Fragments arranged by size:
6831 1842 1745 1455 1335 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2938 3697 4597 4706 6159 6491 7478
Size: 666 881 759 900 109 1453 332 987
Cuts at: 7478 8664 10548 11696 1391
Size: 1186 1884 1148 4072
Fragments arranged by size:
4072 1884 1453 1186 1148 987 900 881
759 666 332 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 3296 3954 4086 4272 6621 9759
Size: 971 299 1098 658 132 186 2349 3138
Cuts at: 9759 10245 10263 928
Size: 486 18 5042
Fragments arranged by size:
5042 3138 2349 1098 971 658 486 299
186 132 18
BbvCI CC'TCA_GC
Cuts at: 8145 8145
Size: 14377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3260 3294 12568 12602 1903
Size: 34 1323 34 9274 34 3678
Fragments arranged by size:
9274 3678 1323 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3187 8980 11587 12269 13479 3187
Size: 5793 2607 682 1210 4085
Fragments arranged by size:
5793 4085 2607 1210 682
BclI T'GATC_A
Cuts at: 1548 3784 8563 1548
Size: 2236 4779 7362
Fragments arranged by size:
7362 4779 2236
BfrBI ATG'CAT
Cuts at: 2878 2878
Size: 14377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 12988 136
Size: 107 122 71 631 605 11316 1525
Fragments arranged by size:
11316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2287 3451 4979 5789 6391 6422 7849 14372 2287
Size: 1164 1528 810 602 31 1427 6523 2292
Fragments arranged by size:
6523 2292 1528 1427 1164 810 602 31
BlpI GC'TnA_GC
Cuts at: 9930 11794 9930
Size: 1864 12513
Bme1580I G_kGCm'C
Cuts at: 1790 3072 4218 4706 5488 8664 12438 13594 1790
Size: 1282 1146 488 782 3176 3774 1156 2573
Fragments arranged by size:
3774 3176 2573 1282 1156 1146 782 488
BmgBI CAC'GTC
Cuts at: 1318 2026 1318
Size: 708 13669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4654 4700 6268
Size: 416 53 83 399 24 3237 46 1568
Cuts at: 6268 7481 8667 9636 10660 12108 13066 442
Size: 1213 1186 969 1024 1448 958 1753
Fragments arranged by size:
3237 1753 1568 1448 1213 1186 1024 969
958 416 399 83 53 46 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 14377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 6505 4304
Size: 2201 12176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 4241 7947 9150 10599 10650 10800 11129
Size: 1430 1572 3706 1203 1449 51 150 329
Cuts at: 11129 11328 13037 1239
Size: 199 1709 2579
Fragments arranged by size:
3706 2579 1709 1572 1449 1430 1203 329
199 150 51
Bpu10I CC'TnA_GC
Cuts at: 7776 8145 7776
Size: 369 14008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 8889 12502 13367 13665 3394
Size: 5495 3613 865 298 4106
Fragments arranged by size:
5495 4106 3613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5213 10923 11180 13040 882
Size: 617 3714 5710 257 1860 2219
Fragments arranged by size:
5710 3714 2219 1860 617 257
BsaAI yAC'GTr
Cuts at: 493 1204 12168 493
Size: 711 10964 2702
Fragments arranged by size:
10964 2702 711
BsaBI GATnn'nnATC
Cuts at: 4185 4566 14371 4185
Size: 381 9805 4191
Fragments arranged by size:
9805 4191 381
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2496 5948 12184 12566
Size: 53 83 186 936 963 3452 6236 382
Cuts at: 12566 13201 275
Size: 635 1451
Fragments arranged by size:
6236 3452 1451 963 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6709 7982 10911 12810 13208 13482 13629 14040 6709
Size: 1273 2929 1899 398 274 147 411 7046
Fragments arranged by size:
7046 2929 1899 1273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3512 3542 4330 4360 4939
Size: 30 165 30 2109 30 788 30 579
Cuts at: 4939 4969 6815 6845 6992 7022 10623 10653 1178
Size: 30 1846 30 147 30 3601 30 4902
Fragments arranged by size:
4902 3601 2109 1846 788 579 165 147
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 9525 10031 11391 1186
Size: 8339 506 1360 4172
Fragments arranged by size:
8339 4172 1360 506
BseYI C'CCAG_C
Cuts at: 13580 14076 13580
Size: 496 13881
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2780 1163
Size: 573 333 711 12760
Fragments arranged by size:
12760 711 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8616 11827 12588 13616 1540
Size: 275 186 6615 3211 761 1028 2301
Fragments arranged by size:
6615 3211 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2800 2938 3292 6159 6491 11299 12438 12523 13594
Size: 138 354 2867 332 4808 1139 85 1071
Cuts at: 13594 2800
Size: 3583
Fragments arranged by size:
4808 3583 2867 1139 1071 354 332 138
85
BsmI GAATG_Cn'
Cuts at: 3749 4127 6103 8226 8432 9605 11888 11981 3749
Size: 378 1976 2123 206 1173 2283 93 6145
Fragments arranged by size:
6145 2283 2123 1976 1173 378 206 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4350 5701 4350
Size: 1351 13026
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2800 2938 3072 3292 3697 4218
Size: 399 267 743 138 134 220 405 521
Cuts at: 4218 4597 4706 5488 6159 6491 7478 8664 10548
Size: 379 109 782 671 332 987 1186 1884
Cuts at: 10548 11299 11696 12438 12523 13594 1391
Size: 751 397 742 85 1071 2174
Fragments arranged by size:
2174 1884 1186 1071 987 782 751 743
742 671 521 405 399 397 379 332
267 220 138 134 109 85
BspEI T'CCGG_A
Cuts at: 6709 14040 6709
Size: 7331 7046
BspHI T'CATG_A
Cuts at: 5464 5989 12266 13996 5464
Size: 525 6277 1730 5845
Fragments arranged by size:
6277 5845 1730 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3727 6544 6565 6946 7123 7297 11732 844
Size: 2883 2817 21 381 177 174 4435 3489
Fragments arranged by size:
4435 3489 2883 2817 381 177 174 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 6795 6972 7149 12264 1394
Size: 429 209 4763 177 177 5115 3507
Fragments arranged by size:
5115 4763 3507 429 209 177 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 3341 4832 5662 6309 12872 13046 65
Size: 1183 2093 1491 830 647 6563 174 1396
Fragments arranged by size:
6563 2093 1491 1396 1183 830 647 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8615 10673 13021 13208 1700
Size: 110 6805 2058 2348 187 2869
Fragments arranged by size:
6805 2869 2348 2058 187 110
BsrGI T'GTAC_A
Cuts at: 96 2626 3575 3730 7378 96
Size: 2530 949 155 3648 7095
Fragments arranged by size:
7095 3648 2530 949 155
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 14377
BssSI C'ACGA_G
Cuts at: 12437 13449 12437
Size: 1012 13365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3076 4765 4970 6657 6846 7023 7200 7853
Size: 2282 1689 205 1687 189 177 177 653
Cuts at: 7853 794
Size: 7318
Fragments arranged by size:
7318 2282 1689 1687 653 205 189 177
177
BstBI TT'CG_AA
Cuts at: 10035 13181 10035
Size: 3146 11231
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 14377
BstXI CCAn_nnnn'nTGG
Cuts at: 4410 7799 11197 4410
Size: 3389 3398 7590
Fragments arranged by size:
7590 3398 3389
BstYI r'GATC_y
Cuts at: 1972 2179 2287 3451 4979 5789 6347 6391 6422
Size: 207 108 1164 1528 810 558 44 31
Cuts at: 6422 7849 9704 10914 11707 12074 12459 12476 13928
Size: 1427 1855 1210 793 367 385 17 1452
Cuts at: 13928 14014 14026 14372 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1855 1528 1452 1427 1210 1164 810
793 558 385 367 346 207 108 86
44 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 3189 5868 3189
Size: 2679 11698
BtgI C'CryG_G
Cuts at: 513 1065 5407 5488 5943 7804 11481 513
Size: 552 4342 81 455 1861 3677 3409
Fragments arranged by size:
4342 3677 3409 1861 552 455 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 3228 8043 487
Size: 683 56 797 1205 4815 6821
Fragments arranged by size:
6821 4815 1205 797 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 6466 6847 7024 7201 9531 11073 11893 12687
Size: 3903 381 177 177 2330 1542 820 794
Cuts at: 12687 12707 2563
Size: 20 4233
Fragments arranged by size:
4233 3903 2330 1542 820 794 381 177
177 20
ClaI AT'CG_AT
Cuts at: 1554 6223 1554
Size: 4669 9708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9507 9542 529
Size: 35 8943 35 5364
Fragments arranged by size:
8943 5364 35 35
DraI TTT'AAA
Cuts at: 3111 5098 5887 5919 6376 7528 7699 8383 8818
Size: 1987 789 32 457 1152 171 684 435
Cuts at: 8818 9274 9838 10807 10942 11077 11681 3111
Size: 456 564 969 135 135 604 5807
Fragments arranged by size:
5807 1987 1152 969 789 684 604 564
457 456 435 171 135 135 32
DraIII CAC_nnn'GTG
Cuts at: 9395 9395
Size: 14377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 13384 809
Size: 507 12068 1802
Fragments arranged by size:
12068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6382 7789 7801
Size: 54 1425 50 275 18 4552 1407 12
Cuts at: 7801 7819 7962 8613 11484 11824 12713 14342 8
Size: 18 143 651 2871 340 889 1629 43
Fragments arranged by size:
4552 2871 1629 1425 1407 889 651 340
275 143 54 50 43 18 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 8613 11824 1537
Size: 275 6801 3211 4090
Fragments arranged by size:
6801 4090 3211 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2335 2805 5541 6949 7126 7300 7504 8227
Size: 982 470 2736 1408 177 174 204 723
Cuts at: 8227 8519 8907 10008 10161 10921 12307 1353
Size: 292 388 1101 153 760 1386 3423
Fragments arranged by size:
3423 2736 1408 1386 1101 982 760 723
470 388 292 204 177 174 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11254 11421 12964 13338 13484 387
Size: 1064 138 9665 167 1543 374 146 1280
Fragments arranged by size:
9665 1543 1280 1064 374 167 146 138
EcoICRI GAG'CTC
Cuts at: 2936 6157 6489 2936
Size: 3221 332 10824
Fragments arranged by size:
10824 3221 332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2786 3290 3734 4128 4241 4608
Size: 393 1037 117 504 444 394 113 367
Cuts at: 4608 5202 6564 6753 6930 7107 7443 7851 7947
Size: 594 1362 189 177 177 336 408 96
Cuts at: 7947 8309 8421 8535 8794 8856 9150 9465 9753
Size: 362 112 114 259 62 294 315 288
Cuts at: 9753 9894 10374 10404 10599 10650 10800 11129 11275
Size: 141 480 30 195 51 150 329 146
Cuts at: 11275 11328 11552 12440 13037 13824 14092 1239
Size: 53 224 888 597 787 268 1524
Fragments arranged by size:
1524 1362 1037 888 787 597 594 504
480 444 408 394 393 367 362 336
329 315 294 288 268 259 224 195
189 177 177 150 146 141 117 114
113 112 96 62 53 51 30
EcoO109I rG'GnC_Cy
Cuts at: 4283 4702 4283
Size: 419 13958
EcoRI G'AATT_C
Cuts at: 2614 3337 4426 4860 5368 5582 8405 11332 11686
Size: 723 1089 434 508 214 2823 2927 354
Cuts at: 11686 12064 2614
Size: 378 4927
Fragments arranged by size:
4927 2927 2823 1089 723 508 434 378
354 214
EcoRV GAT'ATC
Cuts at: 4568 9757 10732 11383 4568
Size: 5189 975 651 7562
Fragments arranged by size:
7562 5189 975 651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4256 5073 5897 6366 7851 8093 4256
Size: 817 824 469 1485 242 10540
Fragments arranged by size:
10540 1485 824 817 469 242
FspI TGC'GCA
Cuts at: 12883 12883
Size: 14377
HaeII r_GCGC'y
Cuts at: 1678 13524 1678
Size: 11846 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 4807 4839 4951
Size: 32 135 32 1250 32 1953 32 112
Cuts at: 4951 4983 5609 5641 6407 6439 7659 7691 8548
Size: 32 626 32 766 32 1220 32 857
Cuts at: 8548 8580 8755 8787 8876 8908 9340 9372 9869
Size: 32 175 32 89 32 432 32 497
Cuts at: 9869 9901 10250 10282 11367 11399 13018 13050 13092
Size: 32 349 32 1085 32 1619 32 42
Cuts at: 13092 13124 1373
Size: 32 2626
Fragments arranged by size:
2626 1953 1619 1250 1220 1085 857 766
626 497 432 349 175 135 112 89
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7656 8793 10464 11721 11967 669
Size: 988 281 5718 1137 1671 1257 246 3079
Fragments arranged by size:
5718 3079 1671 1257 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6366 7548 748
Size: 5618 1182 7577
Fragments arranged by size:
7577 5618 1182
HpaI GTT'AAC
Cuts at: 11967 11967
Size: 14377
KpnI G_GTAC'C
Cuts at: 10367 11702 10367
Size: 1335 13042
MfeI C'AATT_G
Cuts at: 11976 11976
Size: 14377
MluI A'CGCG_T
Cuts at: 14253 14253
Size: 14377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6575 9830 9947 9992 10639 10849 13491 13675
Size: 5270 3255 117 45 647 210 2642 184
Cuts at: 13675 1305
Size: 2007
Fragments arranged by size:
5270 3255 2642 2007 647 210 184 117
45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6384 7791 7821 7964 11486
Size: 54 1425 343 4552 1407 30 143 3522
Cuts at: 11486 10
Size: 2901
Fragments arranged by size:
4552 3522 2901 1425 1407 343 143 54
30
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2568 4408 4746 5493 7679 8320
Size: 648 999 403 1840 338 747 2186 641
Cuts at: 8320 8671 12335 12694 12853 518
Size: 351 3664 359 159 2042
Fragments arranged by size:
3664 2186 2042 1840 999 747 648 641
403 359 351 338 159
MspA1I CmG'CkG
Cuts at: 1606 3765 6084 8119 8434 9929 10043 10637 11793
Size: 2159 2319 2035 315 1495 114 594 1156
Cuts at: 11793 12470 13618 13863 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2319 2159 2120 2035 1495 1156 1148 677
594 315 245 114
NcoI C'CATG_G
Cuts at: 513 1065 5407 5488 5943 11481 513
Size: 552 4342 81 455 5538 3409
Fragments arranged by size:
5538 4342 3409 552 455 81
NdeI CA'TA_TG
Cuts at: 387 2150 387
Size: 1763 12614
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 14377
NotI GC'GGCC_GC
Cuts at: 11824 11824
Size: 14377
NsiI A_TGCA'T
Cuts at: 2880 2880
Size: 14377
NspI r_CATG'y
Cuts at: 1132 1229 2878 2882 3076 5807 6468 6622 7595
Size: 97 1649 4 194 2731 661 154 973
Cuts at: 7595 8738 9225 11159 11564 11735 13280 1132
Size: 1143 487 1934 405 171 1545 2229
Fragments arranged by size:
2731 2229 1934 1649 1545 1143 973 661
487 405 194 171 154 97 4
PasI CC'CwG_GG
Cuts at: 1348 4872 1348
Size: 3524 10853
PciI A'CATG_T
Cuts at: 3072 6618 11155 13276 3072
Size: 3546 4537 2121 4173
Fragments arranged by size:
4537 4173 3546 2121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4712 5267 5634 5777 7322 1108
Size: 3604 555 367 143 1545 8163
Fragments arranged by size:
8163 3604 1545 555 367 143
PmeI GTTT'AAAC
Cuts at: 11681 11681
Size: 14377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6794 6826 6971 7003 7148 7180 12446 12478 13959
Size: 32 145 32 145 32 5266 32 1481
Cuts at: 13959 13991 6794
Size: 32 7180
Fragments arranged by size:
7180 5266 1481 145 145 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 4283 4283
Size: 14377
PshAI GACnn'nnGTC
Cuts at: 1613 2077 1613
Size: 464 13913
PsiI TTA'TAA
Cuts at: 2400 11525 11947 2400
Size: 9125 422 4830
Fragments arranged by size:
9125 4830 422
PspOMI G'GGCC_C
Cuts at: 4702 8660 4702
Size: 3958 10419
PstI C_TGCA'G
Cuts at: 830 4987 5179 6928 7105 11729 830
Size: 4157 192 1749 177 4624 3478
Fragments arranged by size:
4624 4157 3478 1749 192 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14377
PvuII CAG'CTG
Cuts at: 6084 10043 10637 6084
Size: 3959 594 9824
Fragments arranged by size:
9824 3959 594
SacI G_AGCT'C
Cuts at: 2938 6159 6491 2938
Size: 3221 332 10824
Fragments arranged by size:
10824 3221 332
SalI G'TCGA_C
Cuts at: 1936 11719 1936
Size: 9783 4594
SapI GCTCTTCn'nnn_
Cuts at: 2335 2805 2335
Size: 470 13907
SbfI CC_TGCA'GG
Cuts at: 11729 11729
Size: 14377
ScaI AGT'ACT
Cuts at: 8244 8930 12625 14337 8244
Size: 686 3695 1712 8284
Fragments arranged by size:
8284 3695 1712 686
SexAI A'CCwGG_T
Cuts at: 10932 10932
Size: 14377
SfcI C'TryA_G
Cuts at: 826 1045 4270 4983 4998 5175 5245 5526 6924
Size: 219 3225 713 15 177 70 281 1398
Cuts at: 6924 7101 9022 9278 9408 10222 10452 11725 12860
Size: 177 1921 256 130 814 230 1273 1135
Cuts at: 12860 13541 13732 826
Size: 681 191 1471
Fragments arranged by size:
3225 1921 1471 1398 1273 1135 814 713
681 281 256 230 219 191 177 177
130 70 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14377
SmaI CCC'GGG
Cuts at: 1872 11704 1872
Size: 9832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2636 3085 3409 8904 12481 13382
Size: 197 926 693 449 324 5495 3577 901
Cuts at: 13382 13644 820
Size: 262 1553
Fragments arranged by size:
5495 3577 1553 926 901 693 449 324
262 197
SnaBI TAC'GTA
Cuts at: 493 12168 493
Size: 11675 2702
SpeI A'CTAG_T
Cuts at: 152 9683 152
Size: 9531 4846
SphI G_CATG'C
Cuts at: 1132 2882 5807 11735 1132
Size: 1750 2925 5928 3774
Fragments arranged by size:
5928 3774 2925 1750
SspI AAT'ATT
Cuts at: 5 52 12301 5
Size: 47 12249 2081
Fragments arranged by size:
12249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 14377
StyI C'CwwG_G
Cuts at: 513 1065 3469 5407 5488 5943 10098 10498 11371
Size: 552 2404 1938 81 455 4155 400 873
Cuts at: 11371 11481 11816 513
Size: 110 335 3074
Fragments arranged by size:
4155 3074 2404 1938 873 552 455 400
335 110 81
SwaI ATTT'AAAT
Cuts at: 6376 7699 6376
Size: 1323 13054
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2810 3066 5452 9357 12404 12421 12574
Size: 1255 1230 256 2386 3905 3047 17 153
Cuts at: 12574 12759 13204 325
Size: 185 445 1498
Fragments arranged by size:
3905 3047 2386 1498 1255 1230 445 256
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2153 2626 3575
Size: 274 80 33 51 1443 176 473 949
Cuts at: 3575 3611 3730 7378 8242 8731 8928 12623 14335
Size: 36 119 3648 864 489 197 3695 1712
Cuts at: 14335 96
Size: 138
Fragments arranged by size:
3695 3648 1712 1443 949 864 489 473
274 197 176 138 119 80 51 36
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2605 2637 3512 3544 4939 4971 5629 5661 6434
Size: 32 875 32 1395 32 658 32 773
Cuts at: 6434 6466 6626 6658 6815 6847 6992 7024 7169
Size: 32 160 32 157 32 145 32 145
Cuts at: 7169 7201 7497 7529 9208 9240 10623 10655 14337
Size: 32 296 32 1679 32 1383 32 3682
Cuts at: 14337 14369 2605
Size: 32 2613
Fragments arranged by size:
3682 2613 1679 1395 1383 875 773 658
296 160 157 145 145 32 32 32
32 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 14377
XbaI T'CTAG_A
Cuts at: 2419 11713 2419
Size: 9294 5083
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3704 3704
Size: 14377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 14377
XmaI C'CCGG_G
Cuts at: 1870 11702 1870
Size: 9832 4545
XmnI GAAnn'nnTTC
Cuts at: 2090 2415 3462 3747 7878 8103 8540 10260 10716
Size: 325 1047 285 4131 225 437 1720 456
Cuts at: 10716 12506 2090
Size: 1790 3961
Fragments arranged by size:
4131 3961 1790 1720 1047 456 437 325
285 225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2497 12185 276
Size: 53 83 186 936 963 9688 2468
Fragments arranged by size:
9688 2468 963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI