Restriction map for
pFN21AE2353
(Circular) (Six-base) MAPSORT of: pFN21AE2353.seq Check: 833 from: 1 to: 5704
pFN21AE2353 5704 bp
With 182 enzymes: SgfI *
June 2, 2011 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 2780 3767 5151 5419 1632
Size: 583 565 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 583 565 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2077 4603 5580 1203
Size: 874 2526 977 1327
Fragments arranged by size:
2526 1327 977 874
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2023 3447 5019 2023
Size: 1424 1572 2708
Fragments arranged by size:
2708 1572 1424
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 3029 1870
Size: 73 349 737 4545
Fragments arranged by size:
4545 737 349 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3025 4480 618
Size: 325 217 627 665 573 1455 1842
Fragments arranged by size:
1842 1455 665 627 573 325 217
BanII G_rGCy'C
Cuts at: 1391 2144 2387 3023 1391
Size: 753 243 636 4072
Fragments arranged by size:
4072 753 636 243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3895 3929 1903
Size: 34 50 34 1874 34 3678
Fragments arranged by size:
3678 1874 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2391 4315 136
Size: 107 122 71 631 605 719 1924 1525
Fragments arranged by size:
1924 1525 719 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 5699 2100
Size: 3599 2105
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2750 2940 3765 4921 1790
Size: 341 619 190 825 1156 2573
Fragments arranged by size:
2573 1156 825 619 341 190
BmgBI CAC'GTC
Cuts at: 1318 2080 1318
Size: 762 4942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2753 2899 3435
Size: 416 53 83 399 24 1336 146 536
Cuts at: 3435 4393 442
Size: 958 1753
Fragments arranged by size:
1753 1336 958 536 416 399 146 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2033 1056
Size: 977 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2445 2828 4364 1239
Size: 1206 383 1536 2579
Fragments arranged by size:
2579 1536 1206 383
Bpu10I CC'TnA_GC
Cuts at: 2143 2143
Size: 5704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2582 4367 882
Size: 617 1083 1785 2219
Fragments arranged by size:
2219 1785 1083 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 493
Size: 711 2291 2702
Fragments arranged by size:
2702 2291 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 3511 3893 4528
Size: 53 83 186 936 987 991 382 635
Cuts at: 4528 275
Size: 1451
Fragments arranged by size:
1451 991 987 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2837 1186
Size: 1651 4053
BseYI C'CCAG_C
Cuts at: 2175 2645 4907 5403 2175
Size: 470 2262 496 2476
Fragments arranged by size:
2476 2262 496 470
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 2371 3215 3308 2371
Size: 844 93 4767
Fragments arranged by size:
4767 844 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2086 2086
Size: 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2144 2387 2750 2940 3023 3765
Size: 399 341 13 243 363 190 83 742
Cuts at: 3765 3850 4921 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 363 341 243 190
85 83 13
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 4199 4373 65
Size: 1183 994 1957 174 1396
Fragments arranged by size:
1957 1396 1183 994 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4348 4535 1700
Size: 110 2538 187 2869
Fragments arranged by size:
2869 2538 187 110
BsrGI T'GTAC_A
Cuts at: 96 2602 96
Size: 2506 3198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2130 3764 4776 2130
Size: 1634 1012 3058
Fragments arranged by size:
3058 1634 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2023 794
Size: 1229 4475
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2628 2628
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2100 3034 3401 3786 3803 5255 5341 5353
Size: 128 934 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 934 385 367 346 128 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3220 4014 4034 2980
Size: 240 794 20 4650
Fragments arranged by size:
4650 794 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2859 4711 809
Size: 507 1543 1852 1802
Fragments arranged by size:
1852 1802 1543 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3151 4040
Size: 54 1425 50 275 18 829 492 889
Cuts at: 4040 5669 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 829 492 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2235 3634 1353
Size: 687 195 1399 3423
Fragments arranged by size:
3423 1399 687 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2445 2780 2828 3767 4364 5151
Size: 393 583 230 335 48 939 597 787
Cuts at: 5151 5419 1239
Size: 268 1524
Fragments arranged by size:
1524 939 787 597 583 393 335 268
230 48
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2571 2245
Size: 326 5378
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 4851 1678
Size: 3173 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2776 2801 2808
Size: 32 135 32 599 32 573 25 7
Cuts at: 2808 2833 4345 4377 4419 4451 1373
Size: 25 1512 32 42 32 2626
Fragments arranged by size:
2626 1512 599 573 135 42 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 3048 3294 669
Size: 988 281 178 932 246 3079
Fragments arranged by size:
3079 988 932 281 246 178
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 10
Size: 54 1425 343 829 3053
Fragments arranged by size:
3053 1425 829 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2511 3662 4021 4180 518
Size: 648 1345 1151 359 159 2042
Fragments arranged by size:
2042 1345 1151 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2665 3120 3797 4945 5190 1606
Size: 1059 455 677 1148 245 2120
Fragments arranged by size:
2120 1148 1059 677 455 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 2029 1052
Size: 977 4727
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2468 2913 1108
Size: 1360 445 3899
Fragments arranged by size:
3899 1360 445
PfoI T'CCnGG_A
Cuts at: 2580 2805 2580
Size: 225 5479
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2019 3056 830
Size: 1189 1037 3478
Fragments arranged by size:
3478 1189 1037
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2665 2665
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SapI GCTCTTCn'nnn_
Cuts at: 2040 2040
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2015 3052 4187 4868 5059 826
Size: 219 970 1037 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 970 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5704
StyI C'CwwG_G
Cuts at: 513 1065 2220 2650 2922 2940 3143 513
Size: 552 1155 430 272 18 203 3074
Fragments arranged by size:
3074 1155 552 430 272 203 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 2701 3731 3748 3901 4086 4531
Size: 1255 860 261 1030 17 153 185 445
Cuts at: 4531 325
Size: 1498
Fragments arranged by size:
1498 1255 1030 860 445 261 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 2602 3950
Size: 274 80 33 51 1443 367 258 1348
Cuts at: 3950 5662 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1348 367 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2888 2920 5664 5696 2888
Size: 32 2744 32 2896
Fragments arranged by size:
2896 2744 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2706 2706
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI