Restriction map for
pFN21AE2350
(Circular) (Six-base) MAPSORT of: pFN21AE2350.seq Check: 2480 from: 1 to: 6043
pFN21AE2350 6043 bp
With 182 enzymes: SgfI *
May 18, 2011 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 3386 1937
Size: 735 714 4594
Fragments arranged by size:
4594 735 714
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2282 2419 2675 2987 3272 4106 5490 5758
Size: 650 137 256 312 285 834 1384 268
Cuts at: 5758 1632
Size: 1917
Fragments arranged by size:
1917 1384 834 650 312 285 268 256
137
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 2426 2930 1166
Size: 1260 504 4279
Fragments arranged by size:
4279 1260 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2874 1184
Size: 1690 4353
AlwNI CAG_nnn'CTG
Cuts at: 3286 3298 3786 5358 3286
Size: 12 488 1572 3971
Fragments arranged by size:
3971 1572 488 12
ApaI G_GGCC'C
Cuts at: 3330 3330
Size: 6043
ApaLI G'TGCA_C
Cuts at: 2849 4100 5256 2849
Size: 1251 1156 3636
Fragments arranged by size:
3636 1251 1156
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 2174 3373 3740 2174
Size: 1199 367 4477
Fragments arranged by size:
4477 1199 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2569 2996 3364 4819 618
Size: 325 217 627 782 427 368 1455 1842
Fragments arranged by size:
1842 1455 782 627 427 368 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2031 3330 3362 1391
Size: 622 18 1299 32 4072
Fragments arranged by size:
4072 1299 622 32 18
BbeI G_GCGC'C
Cuts at: 2573 2573
Size: 6043
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2485 2975 928
Size: 971 586 490 3996
Fragments arranged by size:
3996 971 586 490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 4654 136
Size: 107 122 71 631 605 397 2585 1525
Fragments arranged by size:
2585 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2853 3330 4104 5260 1790
Size: 1063 477 774 1156 2573
Fragments arranged by size:
2573 1156 1063 774 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3774 4732 442
Size: 416 53 83 399 24 2357 958 1753
Fragments arranged by size:
2357 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2374 2608 2668 4703 1239
Size: 1135 234 60 2035 2579
Fragments arranged by size:
2579 2035 1135 234 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4706 882
Size: 617 3207 2219
Fragments arranged by size:
3207 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2570 3850 4232 4867
Size: 53 83 186 936 1037 1280 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1451 1280 1037 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4476 4874 5148 5295 5706 4476
Size: 398 274 147 411 4813
Fragments arranged by size:
4813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2131 3113 3248 1186
Size: 940 5 982 135 3981
Fragments arranged by size:
3981 982 940 135 5
BseYI C'CCAG_C
Cuts at: 5246 5742 5246
Size: 496 5547
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 1163
Size: 573 613 4857
Fragments arranged by size:
4857 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 3493 4254 5282 1540
Size: 275 186 124 1368 761 1028 2301
Fragments arranged by size:
2301 1368 1028 761 275 186 124
BsiHKAI G_wGCw'C
Cuts at: 2013 2031 2360 2385 2853 3275 4104 4189 5260
Size: 18 329 25 468 422 829 85 1071
Cuts at: 5260 2013
Size: 2796
Fragments arranged by size:
2796 1071 829 468 422 329 85 25
18
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2031 2360 2385 2853 3275 3330
Size: 399 223 18 329 25 468 422 55
Cuts at: 3330 3362 4104 4189 5260 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 422 399 329 223
85 55 32 25 18
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 2613 3932 5662 2613
Size: 1319 1730 2994
Fragments arranged by size:
2994 1730 1319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3398 844
Size: 2554 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2121 3112 3930 1394
Size: 429 298 991 818 3507
Fragments arranged by size:
3507 991 818 429 298
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 2589 4538 4712 65
Size: 1183 1063 278 1949 174 1396
Fragments arranged by size:
1949 1396 1183 1063 278 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 4687 4874 1700
Size: 110 314 2563 187 2869
Fragments arranged by size:
2869 2563 314 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 2101 1925
Size: 176 5867
BssSI C'ACGA_G
Cuts at: 2740 4103 5115 2740
Size: 1363 1012 3668
Fragments arranged by size:
3668 1363 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 2823 4847 2823
Size: 2024 4019
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2174 3373 3740 4125 4142 5594 5680 5692
Size: 202 1199 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1199 385 367 346 202 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2841 3118 3225 513
Size: 552 939 837 277 107 3331
Fragments arranged by size:
3331 939 837 552 277 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2636 5050 809
Size: 507 1320 2414 1802
Fragments arranged by size:
2414 1802 1320 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2469 3048
Size: 54 1425 50 275 18 292 347 579
Cuts at: 3048 3157 3214 3490 4379 6008 8
Size: 109 57 276 889 1629 43
Fragments arranged by size:
1629 1425 889 579 347 292 276 275
109 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2122 3490 1537
Size: 275 310 1368 4090
Fragments arranged by size:
4090 1368 310 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2258 2979 3973 1353
Size: 905 721 994 3423
Fragments arranged by size:
3423 994 905 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2029 2011
Size: 18 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2374 2419 2608 2668 2675 2987
Size: 393 650 92 45 189 60 7 312
Cuts at: 2987 3272 4106 4703 5490 5758 1239
Size: 285 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 650 597 393 312 285
268 189 92 60 45 7
EcoO109I rG'GnC_Cy
Cuts at: 2953 3326 2953
Size: 373 5670
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FseI GG_CCGG'CC
Cuts at: 2128 2128
Size: 6043
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2573 5190 1678
Size: 895 2617 2531
Fragments arranged by size:
2617 2531 895
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2442 2474 4684 4716 4758
Size: 32 135 32 870 32 2210 32 42
Cuts at: 4758 4790 1373
Size: 32 2626
Fragments arranged by size:
2626 2210 870 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 2569 2569
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 2892 3288 5157 5341 1305
Size: 699 888 396 1869 184 2007
Fragments arranged by size:
2007 1869 888 699 396 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 3050 3216 10
Size: 54 1425 343 639 579 166 2837
Fragments arranged by size:
2837 1425 639 579 343 166 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2426 2930 4001 4360 4519 518
Size: 648 1260 504 1071 359 159 2042
Fragments arranged by size:
2042 1260 1071 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2200 2274 2843 3298 3459 4136 5284 5529
Size: 594 74 569 455 161 677 1148 245
Cuts at: 5529 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 594 569 455 245 161
74
NaeI GCC'GGC
Cuts at: 2126 2126
Size: 6043
NarI GG'CG_CC
Cuts at: 2570 2570
Size: 6043
NcoI C'CATG_G
Cuts at: 513 1065 2004 3118 3225 513
Size: 552 939 1114 107 3331
Fragments arranged by size:
3331 1114 939 552 107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NgoMIV G'CCGG_C
Cuts at: 2124 2124
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 3401 4946 1132
Size: 97 2172 1545 2229
Fragments arranged by size:
2229 2172 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2809 3040 1108
Size: 1701 231 4111
Fragments arranged by size:
4111 1701 231
PfoI T'CCnGG_A
Cuts at: 2802 2802
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2953 2953
Size: 6043
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspOMI G'GGCC_C
Cuts at: 3326 3326
Size: 6043
PstI C_TGCA'G
Cuts at: 830 2257 2376 2450 3291 3395 830
Size: 1427 119 74 841 104 3478
Fragments arranged by size:
3478 1427 841 119 104 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 3298 3298
Size: 6043
SacI G_AGCT'C
Cuts at: 2013 2031 2013
Size: 18 6025
SacII CC_GC'GG
Cuts at: 2844 2844
Size: 6043
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SapI GCTCTTCn'nnn_
Cuts at: 2258 2258
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2253 2372 2446 3287 3391 4526 5207
Size: 219 1208 119 74 841 104 1135 681
Cuts at: 5207 5398 826
Size: 191 1471
Fragments arranged by size:
1471 1208 1135 841 681 219 191 119
104 74
SfoI GGC'GCC
Cuts at: 2571 2571
Size: 6043
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6043
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2898 3045 3118 3225 3482
Size: 552 939 357 537 147 73 107 257
Cuts at: 3482 513
Size: 3074
Fragments arranged by size:
3074 939 552 537 357 257 147 107
73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 4070 4087 4240 4425 4870 325
Size: 1255 672 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1255 672 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6001 96
Size: 274 80 33 51 1443 2312 1712 138
Fragments arranged by size:
2312 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6003 6035 6003
Size: 32 6011
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2144 2407 2144
Size: 263 5780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI