Restriction map for
pFN21AE2349
(Circular) (Six-base) MAPSORT of: pFN21AE2349.seq Check: 5258 from: 1 to: 5647
pFN21AE2349 5647 bp
With 182 enzymes: SgfI *
May 20, 2011 16:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2196 2990 1937
Size: 259 794 4594
Fragments arranged by size:
4594 794 259
Acc65I G'GTAC_C
Cuts at: 2810 2968 2810
Size: 158 5489
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 3710 5094 5362 1632
Size: 869 1209 1384 268 1917
Fragments arranged by size:
1917 1384 1209 869 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2127 2273 2379 1166
Size: 961 146 106 4434
Fragments arranged by size:
4434 961 146 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2291 1184
Size: 1107 4540
AlwNI CAG_nnn'CTG
Cuts at: 2020 2625 2647 2717 3390 4962 2020
Size: 605 22 70 673 1572 2705
Fragments arranged by size:
2705 1572 673 605 70 22
ApaI G_GGCC'C
Cuts at: 2034 2034
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2972 1870
Size: 73 83 946 4545
Fragments arranged by size:
4545 946 83 73
AvrII C'CTAG_G
Cuts at: 2539 2539
Size: 5647
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2343 2361 2450 2684 2810
Size: 325 217 627 556 18 89 234 126
Cuts at: 2810 2968 4423 618
Size: 158 1455 1842
Fragments arranged by size:
1842 1455 627 556 325 234 217 158
126 89 18
BanII G_rGCy'C
Cuts at: 1391 2012 2034 2144 2848 2966 1391
Size: 621 22 110 704 118 4072
Fragments arranged by size:
4072 704 621 118 110 22
BbeI G_GCGC'C
Cuts at: 2347 2347
Size: 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4676
BbvCI CC'TCA_GC
Cuts at: 2595 2595
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2029 4258 136
Size: 107 122 71 631 605 357 2229 1525
Fragments arranged by size:
2229 1525 631 605 357 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 2618 3064 2618
Size: 446 5201
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2134 3708 4864 1790
Size: 244 100 1574 1156 2573
Fragments arranged by size:
2573 1574 1156 244 100
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2885 3378 4336
Size: 416 53 83 399 24 1468 493 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1468 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2510 2562 2811 2862 4307 1239
Size: 917 354 52 249 51 1445 2579
Fragments arranged by size:
2579 1445 917 354 249 52 51
Bpu10I CC'TnA_GC
Cuts at: 2595 2595
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2629 4310 882
Size: 617 1130 1681 2219
Fragments arranged by size:
2219 1681 1130 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 2344 3454 3836
Size: 53 83 186 936 760 51 1110 382
Cuts at: 3836 4471 275
Size: 635 1451
Fragments arranged by size:
1451 1110 936 760 635 382 186 83
53 51
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2189 2191 2219 1178
Size: 30 165 30 758 28 2 28 4606
Fragments arranged by size:
4606 758 165 30 30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5647
BseYI C'CCAG_C
Cuts at: 2085 2425 2789 4850 5346 2085
Size: 340 364 2061 496 2386
Fragments arranged by size:
2386 2061 496 364 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 1163
Size: 573 1006 4068
Fragments arranged by size:
4068 1006 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2270 2443 3708 3793 4864 2270
Size: 173 1265 85 1071 3053
Fragments arranged by size:
3053 1265 1071 173 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2034 2134 2144 2270 2443 2848
Size: 399 222 22 100 10 126 173 405
Cuts at: 2848 2966 3708 3793 4864 1391
Size: 118 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 405 399 222 173 126
118 100 85 22 10
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 3002 844
Size: 1487 671 3489
Fragments arranged by size:
3489 1487 671
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4291 4478 1700
Size: 110 2481 187 2869
Fragments arranged by size:
2869 2481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2020 794
Size: 1226 4421
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2381 1184
Size: 1197 4450
BstXI CCAn_nnnn'nTGG
Cuts at: 2358 2443 2358
Size: 85 5562
BstYI r'GATC_y
Cuts at: 1972 2818 2977 3344 3729 3746 5198 5284 5296
Size: 846 159 367 385 17 1452 86 12
Cuts at: 5296 5642 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 846 385 367 346 159 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2472 2754 3163 3957 3977 2472
Size: 282 409 794 20 4142
Fragments arranged by size:
4142 794 409 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DraIII CAC_nnn'GTG
Cuts at: 2189 2189
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2316 2766 2886
Size: 54 1425 50 275 18 486 450 120
Cuts at: 2886 2931 3094 3983 5612 8
Size: 45 163 889 1629 43
Fragments arranged by size:
1629 1425 889 486 450 275 163 120
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 3577 1353
Size: 1169 1055 3423
Fragments arranged by size:
3423 1169 1055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2501 2510 2562 2811 2862 3710
Size: 393 524 345 9 52 249 51 848
Cuts at: 3710 4307 5094 5362 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 848 787 597 524 393 345 268
249 52 51 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2719 2719
Size: 5647
EcoO109I rG'GnC_Cy
Cuts at: 2017 2030 2485 2644 2836 2017
Size: 13 455 159 192 4828
Fragments arranged by size:
4828 455 192 159 13
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2347 4794 1678
Size: 669 2447 2531
Fragments arranged by size:
2531 2447 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2245 2277 4288
Size: 32 135 32 617 32 24 32 2011
Cuts at: 4288 4320 4362 4394 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2011 617 135 42 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2343 2343
Size: 5647
KpnI G_GTAC'C
Cuts at: 2814 2972 2814
Size: 158 5489
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4761 4945 1305
Size: 3456 184 2007
Fragments arranged by size:
3456 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 2768 2888 2933 10
Size: 54 1425 343 486 450 120 45 2724
Fragments arranged by size:
2724 1425 486 450 343 120 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 2273 2379 3605 3964 4123 518
Size: 648 961 146 106 1226 359 159 2042
Fragments arranged by size:
2042 1226 961 648 359 159 146 106
MspA1I CmG'CkG
Cuts at: 1606 2273 3063 3740 4888 5133 1606
Size: 667 790 677 1148 245 2120
Fragments arranged by size:
2120 1148 790 677 667 245
NarI GG'CG_CC
Cuts at: 2344 2344
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2836 2836
Size: 5647
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2030 2030
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2237 2245 2999 830
Size: 1407 8 754 3478
Fragments arranged by size:
3478 1407 754 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2273 2273
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SapI GCTCTTCn'nnn_
Cuts at: 2522 2522
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2233 2241 2533 2995 4130 4811 5002
Size: 219 1188 8 292 462 1135 681 191
Cuts at: 5002 826
Size: 1471
Fragments arranged by size:
1471 1188 1135 681 462 292 219 191
8
SfoI GGC'GCC
Cuts at: 2345 2345
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2028 2974 1872
Size: 156 946 4545
Fragments arranged by size:
4545 946 156
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 2783 152
Size: 2631 3016
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2231 2677 1517
Size: 714 446 4487
Fragments arranged by size:
4487 714 446
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2522 2539 2794 2814 2898
Size: 552 939 87 431 17 255 20 84
Cuts at: 2898 3086 513
Size: 188 3074
Fragments arranged by size:
3074 939 552 431 255 188 87 84
20 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3674 3691 3844 4029 4474 325
Size: 1255 1067 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 1027 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2161 2193 5607 5639 2161
Size: 32 3414 32 2169
Fragments arranged by size:
3414 2169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2761 2896 2761
Size: 135 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2026 2972 1870
Size: 156 946 4545
Fragments arranged by size:
4545 946 156
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI