Restriction map for
pFN21AE2345
(Circular) (Six-base) MAPSORT of: pFN21AE2345.seq Check: 9192 from: 1 to: 5782
pFN21AE2345 5782 bp
With 182 enzymes: SgfI *
May 20, 2011 16:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 2913 3048 3103 2913
Size: 135 55 5592
Fragments arranged by size:
5592 135 55
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2977 3845 5229 5497 1632
Size: 1345 868 1384 268 1917
Fragments arranged by size:
1917 1384 1345 868 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 2661 4681 5658 1203
Size: 1458 2020 977 1327
Fragments arranged by size:
2020 1458 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 2077 2638 2926 3525 5097 2077
Size: 561 288 599 1572 2762
Fragments arranged by size:
2762 1572 599 561 288
ApaI G_GGCC'C
Cuts at: 2021 2828 2021
Size: 807 4975
ApaLI G'TGCA_C
Cuts at: 2324 3839 4995 2324
Size: 1515 1156 3111
Fragments arranged by size:
3111 1515 1156
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2374 3107 1870
Size: 73 431 733 4545
Fragments arranged by size:
4545 733 431 73
BamHI G'GATC_C
Cuts at: 2008 3112 3479 2008
Size: 1104 367 4311
Fragments arranged by size:
4311 1104 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 2913 3048 3103 4558
Size: 325 217 627 467 659 135 55 1455
Cuts at: 4558 618
Size: 1842
Fragments arranged by size:
1842 1455 659 627 467 325 217 135
55
BanII G_rGCy'C
Cuts at: 1391 2021 2828 2897 3101 1391
Size: 630 807 69 204 4072
Fragments arranged by size:
4072 807 630 204 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2310 2447 3674 4884 2310
Size: 137 1227 1210 3208
Fragments arranged by size:
3208 1227 1210 137
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5782
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 4393 136
Size: 107 122 71 631 605 1126 1595 1525
Fragments arranged by size:
1595 1525 1126 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2328 2359 2814 2828 3004 3843 4999
Size: 231 307 31 455 14 176 839 1156
Cuts at: 4999 1790
Size: 2573
Fragments arranged by size:
2573 1156 839 455 307 231 176 31
14
BmgBI CAC'GTC
Cuts at: 1318 2208 2662 1318
Size: 890 454 4438
Fragments arranged by size:
4438 890 454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2346 2608 3513
Size: 416 53 83 399 24 929 262 905
Cuts at: 3513 4471 442
Size: 958 1753
Fragments arranged by size:
1753 958 929 905 416 399 262 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2816 4442 1239
Size: 1298 279 1626 2579
Fragments arranged by size:
2579 1626 1298 279
Bpu10I CC'TnA_GC
Cuts at: 2951 2951
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2100 2636 3907 4772 5070 2100
Size: 536 1271 865 298 2812
Fragments arranged by size:
2812 1271 865 536 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2878 3573 493
Size: 711 1674 695 2702
Fragments arranged by size:
2702 1674 711 695
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2865 3589 3971 4606
Size: 53 83 186 936 1332 724 382 635
Cuts at: 4606 275
Size: 1451
Fragments arranged by size:
1451 1332 936 724 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 2657 2687 2699
Size: 30 165 30 1107 30 117 30 12
Cuts at: 2699 2729 1178
Size: 30 4231
Fragments arranged by size:
4231 1107 165 117 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5782
BseYI C'CCAG_C
Cuts at: 2358 2821 2828 2997 4985 5481 2358
Size: 463 7 169 1988 496 2659
Fragments arranged by size:
2659 1988 496 463 169 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2290 3232 3993 5021 1540
Size: 275 186 289 942 761 1028 2301
Fragments arranged by size:
2301 1028 942 761 289 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 3843 3928 4999 2328
Size: 1515 85 1071 3111
Fragments arranged by size:
3111 1515 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
BsmBI CGTCTCn'nnnn_
Cuts at: 2702 2966 2702
Size: 264 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2328 2359 2814 2828 2897 3004
Size: 399 231 307 31 455 14 69 107
Cuts at: 3004 3101 3843 3928 4999 1391
Size: 97 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 399 307 231 107
97 85 69 31 14
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 3671 5401 3671
Size: 1730 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2925 3137 844
Size: 2081 212 3489
Fragments arranged by size:
3489 2081 212
BsrBI CCG'CTC
Cuts at: 1394 1823 2551 3669 1394
Size: 429 728 1118 3507
Fragments arranged by size:
3507 1118 728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4277 4451 65
Size: 1183 3029 174 1396
Fragments arranged by size:
3029 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 2343 96
Size: 2247 3535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3021 794
Size: 2227 3555
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2008 2397 3112 3479 3864 3881 5333 5419
Size: 36 389 715 367 385 17 1452 86
Cuts at: 5419 5431 5777 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 715 389 385 367 346 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2445 2522 2814 513
Size: 552 939 441 77 292 3481
Fragments arranged by size:
3481 939 552 441 292 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 3862
Fragments arranged by size:
3862 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 2425 2595 3298 4092 4112 2280
Size: 145 170 703 794 20 3950
Fragments arranged by size:
3950 794 703 170 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2530 2562
Size: 54 1425 50 275 18 192 508 32
Cuts at: 2562 3229 4118 5747 8
Size: 667 889 1629 43
Fragments arranged by size:
1629 1425 889 667 508 275 192 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3712 1353
Size: 2359 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2602 4369 4743 4889 387
Size: 1064 138 1013 1767 374 146 1280
Fragments arranged by size:
1767 1280 1064 1013 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2816 2977 3845 4442 5229 5497
Size: 393 905 279 161 868 597 787 268
Cuts at: 5497 1239
Size: 1524
Fragments arranged by size:
1524 905 868 787 597 393 279 268
161
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 2690
Size: 5782
EcoO109I rG'GnC_Cy
Cuts at: 2145 2169 2825 2902 2145
Size: 24 656 77 5025
Fragments arranged by size:
5025 656 77 24
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2047 4929 1678
Size: 369 2882 2531
Fragments arranged by size:
2882 2531 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2510 2542 2699 2731 4423
Size: 32 135 32 938 32 157 32 1692
Cuts at: 4423 4455 4497 4529 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1692 938 157 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 2917 3052 3107 2917
Size: 135 55 5592
Fragments arranged by size:
5592 135 55
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 2619 4896 5080 1305
Size: 1209 105 2277 184 2007
Fragments arranged by size:
2277 2007 1209 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3740 4099 4258 518
Size: 648 1710 864 359 159 2042
Fragments arranged by size:
2042 1710 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2241 2437 2997 3198 3875 5023 5268
Size: 468 167 196 560 201 677 1148 245
Cuts at: 5268 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 560 468 245 201 196
167
NcoI C'CATG_G
Cuts at: 513 1065 2004 2445 513
Size: 552 939 441 3850
Fragments arranged by size:
3850 939 552 441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2014 2692 1348
Size: 666 678 4438
Fragments arranged by size:
4438 678 666
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PmlI CAC'GTG
Cuts at: 2878 2878
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 2169 2902 2145
Size: 24 733 5025
Fragments arranged by size:
5025 733 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspOMI G'GGCC_C
Cuts at: 2017 2824 2017
Size: 807 4975
PstI C_TGCA'G
Cuts at: 830 2643 2922 3036 3134 830
Size: 1813 279 114 98 3478
Fragments arranged by size:
3478 1813 279 114 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
PvuII CAG'CTG
Cuts at: 2074 2437 2997 2074
Size: 363 560 4859
Fragments arranged by size:
4859 560 363
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SanDI GG'GwC_CC
Cuts at: 2145 2169 2145
Size: 24 5758
SbfI CC_TGCA'GG
Cuts at: 2643 2922 3134 2643
Size: 279 212 5291
Fragments arranged by size:
5291 279 212
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SfcI C'TryA_G
Cuts at: 826 1045 2639 2918 3032 3130 4265 4946 5137
Size: 219 1594 279 114 98 1135 681 191
Cuts at: 5137 826
Size: 1471
Fragments arranged by size:
1594 1471 1135 681 279 219 191 114
98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2115 2651 3886 4787 5049 820
Size: 197 926 172 536 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 536 262 197 172
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2801 3020 1517
Size: 1284 219 4279
Fragments arranged by size:
4279 1284 219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2445 2898 3221 513
Size: 552 939 441 453 323 3074
Fragments arranged by size:
3074 939 552 453 441 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 2585 3809 3826 3979 4164 4609
Size: 1255 979 26 1224 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 1224 979 445 185 153 26
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 4028 5740
Size: 274 80 33 51 1443 366 1685 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1685 1443 366 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 2315 2713 1316
Size: 999 398 4385
Fragments arranged by size:
4385 999 398
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2037 2427 2572 2011
Size: 26 390 145 5221
Fragments arranged by size:
5221 390 145 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI