Restriction map for
pFN21AE2343
(Circular) (Six-base) MAPSORT of: pFN21AE2343.seq Check: 6508 from: 1 to: 6664
pFN21AE2343 6664 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3526 3526
Size: 6664
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2067 4474 278
Size: 53 83 186 936 531 2407 2468
Fragments arranged by size:
2468 2407 936 531 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3015 4007 1937
Size: 1078 992 4594
Fragments arranged by size:
4594 1078 992
Acc65I G'GTAC_C
Cuts at: 2499 3985 2499
Size: 1486 5178
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2819 4727 6111 6379 1632
Size: 1187 1908 1384 268 1917
Fragments arranged by size:
1917 1908 1384 1187 268
AfeI AGC'GCT
Cuts at: 2189 3321 2189
Size: 1132 5532
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 2847 3961 5563 6540 1203
Size: 1644 1114 1602 977 1327
Fragments arranged by size:
1644 1602 1327 1114 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3338 1184
Size: 2154 4510
AlwNI CAG_nnn'CTG
Cuts at: 2059 3703 4407 5979 2059
Size: 1644 704 1572 2744
Fragments arranged by size:
2744 1644 1572 704
ApaI G_GGCC'C
Cuts at: 2389 2459 2598 2826 3061 3127 3165 3899 2389
Size: 70 139 228 235 66 38 734 5154
Fragments arranged by size:
5154 734 235 228 139 70 66 38
ApaLI G'TGCA_C
Cuts at: 4721 5877 4721
Size: 1156 5508
ApoI r'AATT_y
Cuts at: 1493 3973 4199 4351 6460 1493
Size: 2480 226 152 2109 1697
Fragments arranged by size:
2480 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 2471 2701 2825 3259 3501 3838
Size: 73 348 180 230 124 434 242 337
Cuts at: 3838 3989 1870
Size: 151 4545
Fragments arranged by size:
4545 434 348 337 242 230 180 151
124 73
AvrII C'CTAG_G
Cuts at: 3899 3899
Size: 6664
BamHI G'GATC_C
Cuts at: 2287 3994 4361 2287
Size: 1707 367 4590
Fragments arranged by size:
4590 1707 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2467 2499 2506 2531
Size: 325 217 627 489 191 32 7 25
Cuts at: 2531 2737 2777 3353 3398 3538 3602 3985 5440
Size: 206 40 576 45 140 64 383 1455
Cuts at: 5440 618
Size: 1842
Fragments arranged by size:
1842 1455 627 576 489 383 325 217
206 191 140 64 45 40 32 25
7
BanII G_rGCy'C
Cuts at: 1391 2298 2389 2399 2459 2598 2826 2859 3008
Size: 907 91 10 60 139 228 33 149
Cuts at: 3008 3036 3061 3127 3165 3899 3983 1391
Size: 28 25 66 38 734 84 4072
Fragments arranged by size:
4072 907 734 228 149 139 91 84
66 60 38 33 28 25 10
BbeI G_GCGC'C
Cuts at: 2280 2471 2510 2535 3357 3402 2280
Size: 191 39 25 822 45 5542
Fragments arranged by size:
5542 822 191 45 39 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5693
BbvCI CC'TCA_GC
Cuts at: 2599 2599
Size: 6664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4855 4889 1903
Size: 34 50 34 2834 34 3678
Fragments arranged by size:
3678 2834 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2873 3375 3437
Size: 107 122 71 631 605 1201 502 62
Cuts at: 3437 5275 136
Size: 1838 1525
Fragments arranged by size:
1838 1525 1201 631 605 502 122 107
71 62
BglII A'GATC_T
Cuts at: 6659 6659
Size: 6664
BlpI GC'TnA_GC
Cuts at: 4081 4081
Size: 6664
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2389 2459 2598 2826 3061 3127 3165
Size: 356 243 70 139 228 235 66 38
Cuts at: 3165 3605 3773 3899 4725 5881 1790
Size: 440 168 126 826 1156 2573
Fragments arranged by size:
2573 1156 826 440 356 243 235 228
168 139 126 70 66 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2396 4395 5353
Size: 416 53 83 399 24 979 1999 958
Cuts at: 5353 442
Size: 1753
Fragments arranged by size:
1999 1753 979 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3050 3149 3236 3407 3893 5324 1239
Size: 1811 99 87 171 486 1431 2579
Fragments arranged by size:
2579 1811 1431 486 171 99 87
Bpu10I CC'TnA_GC
Cuts at: 2599 3242 2599
Size: 643 6021
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3188 4789 5654 5952 2978
Size: 210 1601 865 298 3690
Fragments arranged by size:
3690 1601 865 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5327 882
Size: 617 3828 2219
Fragments arranged by size:
3828 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3667 4455 493
Size: 711 2463 788 2702
Fragments arranged by size:
2702 2463 788 711
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2259 2277 2468
Size: 53 83 186 936 531 195 18 191
Cuts at: 2468 2507 2532 2963 2985 3354 3399 4471 4853
Size: 39 25 431 22 369 45 1072 382
Cuts at: 4853 5488 275
Size: 635 1451
Fragments arranged by size:
1451 1072 936 635 531 431 382 369
195 191 186 83 53 45 39 25
22 18
BsaWI w'CCGG_w
Cuts at: 3956 5097 5495 5769 5916 6327 3956
Size: 1141 398 274 147 411 4293
Fragments arranged by size:
4293 1141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 2870 2900 2948
Size: 30 165 30 651 30 786 30 48
Cuts at: 2948 2978 3215 3245 1178
Size: 30 237 30 4597
Fragments arranged by size:
4597 786 651 237 165 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2972 3057 3233 3236 3410 3458 1186
Size: 873 913 85 176 3 174 48 4392
Fragments arranged by size:
4392 913 873 176 174 85 48 3
BseYI C'CCAG_C
Cuts at: 2028 3325 3349 3720 3796 5867 6363 2028
Size: 1297 24 371 76 2071 496 2329
Fragments arranged by size:
2329 2071 1297 496 371 76 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3191 3785 1163
Size: 573 1455 594 4042
Fragments arranged by size:
4042 1455 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2484 2559 2769 3441 3825 4114
Size: 275 186 483 75 210 672 384 289
Cuts at: 4114 4875 5903 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 672 483 384 289 275
210 186 75
BsiHKAI G_wGCw'C
Cuts at: 4725 4810 5881 4725
Size: 85 1071 5508
Fragments arranged by size:
5508 1071 85
BsiWI C'GTAC_G
Cuts at: 2850 2850
Size: 6664
BsmI GAATG_Cn'
Cuts at: 4175 4268 4175
Size: 93 6571
BsmBI CGTCTCn'nnnn_
Cuts at: 2071 2071
Size: 6664
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2298 2389 2399 2459 2598 2826
Size: 399 356 152 91 10 60 139 228
Cuts at: 2826 2859 3008 3036 3061 3127 3165 3605 3773
Size: 33 149 28 25 66 38 440 168
Cuts at: 3773 3899 3983 4725 4810 5881 1391
Size: 126 84 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 440 399 356 228 168
152 149 139 126 91 85 84 66
60 38 33 28 25 10
BspEI T'CCGG_A
Cuts at: 3956 6327 3956
Size: 2371 4293
BspHI T'CATG_A
Cuts at: 4553 6283 4553
Size: 1730 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3526 4019 844
Size: 2682 493 3489
Fragments arranged by size:
3489 2682 493
BsrBI CCG'CTC
Cuts at: 1394 1823 3055 3544 3745 4551 1394
Size: 429 1232 489 201 806 3507
Fragments arranged by size:
3507 1232 806 489 429 201
BsrDI GCAATG_nn'
Cuts at: 65 1248 2449 5159 5333 65
Size: 1183 1201 2710 174 1396
Fragments arranged by size:
2710 1396 1201 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 2438 2483 2734 3642 5308 5495
Size: 110 276 352 45 251 908 1666 187
Cuts at: 5495 1700
Size: 2869
Fragments arranged by size:
2869 1666 908 352 276 251 187 110
45
BsrGI T'GTAC_A
Cuts at: 96 2610 96
Size: 2514 4150
BssHII G'CGCG_C
Cuts at: 1925 2520 2655 3198 3411 3413 3443 3528 3732
Size: 595 135 543 213 2 30 85 204
Cuts at: 3732 1925
Size: 4857
Fragments arranged by size:
4857 595 543 213 204 135 85 30
2
BssSI C'ACGA_G
Cuts at: 3069 4724 5736 3069
Size: 1655 1012 3997
Fragments arranged by size:
3997 1655 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 3703 794
Size: 1654 1255 3755
Fragments arranged by size:
3755 1654 1255
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 3112 3349 3919 3112
Size: 237 570 5857
Fragments arranged by size:
5857 570 237
BstYI r'GATC_y
Cuts at: 1972 2287 3699 3994 4361 4746 4763 6215 6301
Size: 315 1412 295 367 385 17 1452 86
Cuts at: 6301 6313 6659 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1412 385 367 346 315 295
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2763 2871 2898 2952 3278 3302 3618
Size: 552 1698 108 27 54 326 24 316
Cuts at: 3618 3691 513
Size: 73 3486
Fragments arranged by size:
3486 1698 552 326 316 108 73 54
27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5925
Fragments arranged by size:
5925 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 3179 4180 4974 4994 2166
Size: 1013 1001 794 20 3836
Fragments arranged by size:
3836 1013 1001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2120 2155 2959 2994 529
Size: 35 1556 35 804 35 4199
Fragments arranged by size:
4199 1556 804 35 35 35
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2300 2481
Size: 54 1425 50 275 18 215 255 181
Cuts at: 2481 2556 2766 3438 3822 3885 4111 5000 6629
Size: 75 210 672 384 63 226 889 1629
Cuts at: 6629 8
Size: 43
Fragments arranged by size:
1629 1425 889 672 384 275 255 226
215 210 181 75 63 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2481 2556 2766 3438 3822 4111 1537
Size: 275 669 75 210 672 384 289 4090
Fragments arranged by size:
4090 672 669 384 289 275 210 75
EarI CTCTTCn'nnn_
Cuts at: 1353 4594 1353
Size: 3241 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3194 5251 5625 5771 387
Size: 1064 138 1605 2057 374 146 1280
Fragments arranged by size:
2057 1605 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2819 3050 3149 3236 3407 3893 4727
Size: 393 1187 231 99 87 171 486 834
Cuts at: 4727 5324 6111 6379 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1187 834 787 597 486 393 268
231 171 99 87
EcoNI CCTnn'n_nnAGG
Cuts at: 3422 3422
Size: 6664
EcoO109I rG'GnC_Cy
Cuts at: 2385 2386 2455 2595 2822 2866 2998 3058 3123
Size: 1 69 140 227 44 132 60 65
Cuts at: 3123 3124 3162 3868 3896 3903 2385
Size: 1 38 706 28 7 5146
Fragments arranged by size:
5146 706 227 140 132 69 65 60
44 38 28 7 1 1
EcoRI G'AATT_C
Cuts at: 3973 4351 3973
Size: 378 6286
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 2191 2280 2471 2510 2535 2606 3215 3323
Size: 513 89 191 39 25 71 609 108
Cuts at: 3323 3357 3402 3491 3649 3674 3716 3761 3853
Size: 34 45 89 158 25 42 45 92
Cuts at: 3853 3884 5811 1678
Size: 31 1927 2531
Fragments arranged by size:
2531 1927 609 513 191 158 108 92
89 89 71 45 45 42 39 34
31 25 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2054 2086 2141 2173 2870
Size: 32 135 32 482 32 55 32 697
Cuts at: 2870 2902 2948 2980 5305 5337 5379 5411 1373
Size: 32 46 32 2325 32 42 32 2626
Fragments arranged by size:
2626 2325 697 482 135 55 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4008 4254 669
Size: 988 281 2070 246 3079
Fragments arranged by size:
3079 2070 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6664
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KasI G'GCGC_C
Cuts at: 2276 2467 2506 2531 3353 3398 2276
Size: 191 39 25 822 45 5542
Fragments arranged by size:
5542 822 191 45 39 25
KpnI G_GTAC'C
Cuts at: 2503 3989 2503
Size: 1486 5178
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 2847 6540 2847
Size: 3693 2971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2199 5778 5962 1305
Size: 774 120 3579 184 2007
Fragments arranged by size:
3579 2007 774 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2047 2302 3887 10
Size: 54 1425 343 215 255 1585 2787
Fragments arranged by size:
2787 1585 1425 343 255 215 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4622 4981 5140 518
Size: 648 3456 359 159 2042
Fragments arranged by size:
3456 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2608 2900 2954 3280 3304 3333 3349 3620
Size: 1002 292 54 326 24 29 16 271
Cuts at: 3620 3693 3709 3805 4080 4757 5905 6150 1606
Size: 73 16 96 275 677 1148 245 2120
Fragments arranged by size:
2120 1148 1002 677 326 292 275 271
245 96 73 54 29 24 16 16
NaeI GCC'GGC
Cuts at: 2088 2440 2485 2736 3644 2088
Size: 352 45 251 908 5108
Fragments arranged by size:
5108 908 352 251 45
NarI GG'CG_CC
Cuts at: 2277 2468 2507 2532 3354 3399 2277
Size: 191 39 25 822 45 5542
Fragments arranged by size:
5542 822 191 45 39 25
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6112
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NgoMIV G'CCGG_C
Cuts at: 2086 2438 2483 2734 3642 2086
Size: 352 45 251 908 5108
Fragments arranged by size:
5108 908 352 251 45
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 3438 4111 3438
Size: 673 5991
NspI r_CATG'y
Cuts at: 1132 1229 4022 5567 1132
Size: 97 2793 1545 2229
Fragments arranged by size:
2793 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2111 2242 2389 3119 1348
Size: 763 131 147 730 4893
Fragments arranged by size:
4893 763 730 147 131
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2098 1108
Size: 902 88 5674
Fragments arranged by size:
5674 902 88
PfoI T'CCnGG_A
Cuts at: 3048 3048
Size: 6664
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2998 3868 2998
Size: 870 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PspOMI G'GGCC_C
Cuts at: 2385 2455 2594 2822 3057 3123 3161 3895 2385
Size: 70 139 228 235 66 38 734 5154
Fragments arranged by size:
5154 734 235 228 139 70 66 38
PstI C_TGCA'G
Cuts at: 830 2571 3537 3708 3849 4016 830
Size: 1741 966 171 141 167 3478
Fragments arranged by size:
3478 1741 966 171 167 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 3349 3805 3349
Size: 456 6208
RsrII CG'GwC_CG
Cuts at: 3282 3282
Size: 6664
SacII CC_GC'GG
Cuts at: 2901 2955 3281 3305 3621 3694 2901
Size: 54 326 24 316 73 5871
Fragments arranged by size:
5871 326 316 73 54 24
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 2204 4912 6624 2204
Size: 2708 1712 2244
Fragments arranged by size:
2708 2244 1712
SfcI C'TryA_G
Cuts at: 826 1045 2567 3533 3704 3845 4012 5147 5828
Size: 219 1522 966 171 141 167 1135 681
Cuts at: 5828 6019 826
Size: 191 1471
Fragments arranged by size:
1522 1471 1135 966 681 219 191 171
167 141
SfoI GGC'GCC
Cuts at: 2278 2469 2508 2533 3355 3400 2278
Size: 191 39 25 822 45 5542
Fragments arranged by size:
5542 822 191 45 39 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 3642 1700
Size: 1942 4722
SmaI CCC'GGG
Cuts at: 1872 2703 3261 3503 3840 3991 1872
Size: 831 558 242 337 151 4545
Fragments arranged by size:
4545 831 558 337 242 151
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2993 3203 4768 5669 5931 820
Size: 197 926 1050 210 1565 901 262 1553
Fragments arranged by size:
1565 1553 1050 926 901 262 210 197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 2346 1517
Size: 829 5835
StyI C'CwwG_G
Cuts at: 513 1065 2325 2862 3888 3899 3912 4103 513
Size: 552 1260 537 1026 11 13 191 3074
Fragments arranged by size:
3074 1260 1026 552 537 191 13 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4691 4708 4861 5046 5491 325
Size: 1255 3111 17 153 185 445 1498
Fragments arranged by size:
3111 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 2610 4910
Size: 274 80 33 51 1443 225 408 2300
Cuts at: 4910 6622 96
Size: 1712 138
Fragments arranged by size:
2300 1712 1443 408 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 6624 6656 3215
Size: 32 3377 32 3223
Fragments arranged by size:
3377 3223 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2869 2869
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 2701 3259 3501 3838 3989 1870
Size: 831 558 242 337 151 4545
Fragments arranged by size:
4545 831 558 337 242 151
XmnI GAAnn'nnTTC
Cuts at: 4793 4793
Size: 6664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2065 4472 276
Size: 53 83 186 936 531 2407 2468
Fragments arranged by size:
2468 2407 936 531 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI
SwaI