Restriction map for
pFN21AE2337
(Circular) (Six-base) MAPSORT of: pFN21AE2337.seq Check: 4892 from: 1 to: 7306
pFN21AE2337 7306 bp
With 182 enzymes: SgfI *
April 13, 2011 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4019 4019
Size: 7306
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5116 278
Size: 53 83 186 936 3580 2468
Fragments arranged by size:
3580 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2293 2467 4649 1937
Size: 356 174 2182 4594
Fragments arranged by size:
4594 2182 356 174
Acc65I G'GTAC_C
Cuts at: 4627 4627
Size: 7306
AclI AA'CG_TT
Cuts at: 1563 5433 5806 1563
Size: 3870 373 3063
Fragments arranged by size:
3870 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3537 5369 6753 7021 1632
Size: 1905 1832 1384 268 1917
Fragments arranged by size:
1917 1905 1832 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7109
AflIII A'CryG_T
Cuts at: 1203 2730 3158 6205 7182 1203
Size: 1527 428 3047 977 1327
Fragments arranged by size:
3047 1527 1327 977 428
AgeI A'CCGG_T
Cuts at: 6137 6137
Size: 7306
AhdI GACnn_n'nnGTC
Cuts at: 6035 6035
Size: 7306
AleI CACnn'nnGTG
Cuts at: 1166 3600 1166
Size: 2434 4872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4272 1184
Size: 3088 4218
AlwNI CAG_nnn'CTG
Cuts at: 2774 3484 4225 4406 5049 6621 2774
Size: 710 741 181 643 1572 3459
Fragments arranged by size:
3459 1572 741 710 643 181
ApaLI G'TGCA_C
Cuts at: 3637 5363 6519 3637
Size: 1726 1156 4424
Fragments arranged by size:
4424 1726 1156
ApoI r'AATT_y
Cuts at: 1493 2318 2759 2819 3238 3289 3857 4615 4841
Size: 825 441 60 419 51 568 758 226
Cuts at: 4841 4993 7102 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 825 758 568 441 419 226
152 60 51
AseI AT'TA_AT
Cuts at: 160 1032 5860 160
Size: 872 4828 1606
Fragments arranged by size:
4828 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7306
AvaI C'yCGr_G
Cuts at: 1870 1943 3833 4631 1870
Size: 73 1890 798 4545
Fragments arranged by size:
4545 1890 798 73
BamHI G'GATC_C
Cuts at: 4636 5003 4636
Size: 367 6939
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2576 4627 6082 618
Size: 325 217 627 789 2051 1455 1842
Fragments arranged by size:
2051 1842 1455 789 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2219 3629 4625 1391
Size: 828 1410 996 4072
Fragments arranged by size:
4072 1410 996 828
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3114 928
Size: 971 1215 5120
Fragments arranged by size:
5120 1215 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5497 5531 1903
Size: 34 3560 34 3678
Fragments arranged by size:
3678 3560 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5198 6408 3908
Size: 1290 1210 4806
Fragments arranged by size:
4806 1290 1210
BclI T'GATC_A
Cuts at: 1548 2628 1548
Size: 1080 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5917 136
Size: 107 122 71 631 605 4245 1525
Fragments arranged by size:
4245 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3398 7301 3398
Size: 3903 3403
BlpI GC'TnA_GC
Cuts at: 3318 4195 4723 3318
Size: 877 528 5901
Fragments arranged by size:
5901 877 528
Bme1580I G_kGCm'C
Cuts at: 1790 3531 3641 4420 5367 6523 1790
Size: 1741 110 779 947 1156 2573
Fragments arranged by size:
2573 1741 1156 947 779 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7306
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3074 3243 3431
Size: 416 53 83 399 24 1657 169 188
Cuts at: 3431 5037 5995 442
Size: 1606 958 1753
Fragments arranged by size:
1753 1657 1606 958 416 399 188 169
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2331 2772 3058 3822 5966 1239
Size: 1092 441 286 764 2144 2579
Fragments arranged by size:
2579 2144 1092 764 441 286
Bpu10I CC'TnA_GC
Cuts at: 3623 3623
Size: 7306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3967 5431 6296 6594 3573
Size: 394 1464 865 298 4285
Fragments arranged by size:
4285 1464 865 394 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4114 5969 882
Size: 617 2615 1855 2219
Fragments arranged by size:
2615 2219 1855 617
BsaAI yAC'GTr
Cuts at: 493 1204 3161 5097 493
Size: 711 1957 1936 2702
Fragments arranged by size:
2702 1957 1936 711
BsaBI GATnn'nnATC
Cuts at: 7300 7300
Size: 7306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3608 5113 5495 6130
Size: 53 83 186 936 2075 1505 382 635
Cuts at: 6130 275
Size: 1451
Fragments arranged by size:
2075 1505 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5739 6137 6411 6558 6969 5739
Size: 398 274 147 411 6076
Fragments arranged by size:
6076 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 1178
Size: 30 165 30 1188 30 5863
Fragments arranged by size:
5863 1188 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 1186
Size: 1426 5880
BseYI C'CCAG_C
Cuts at: 3506 6509 7005 3506
Size: 3003 496 3807
Fragments arranged by size:
3807 3003 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3279 4268 1163
Size: 573 1543 989 4201
Fragments arranged by size:
4201 1543 989 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4756 5517 6545 1540
Size: 275 186 2755 761 1028 2301
Fragments arranged by size:
2755 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3641 3941 3991 5367 5452 6523 3641
Size: 300 50 1376 85 1071 4424
Fragments arranged by size:
4424 1376 1071 300 85 50
BsmI GAATG_Cn'
Cuts at: 3448 4817 4910 3448
Size: 1369 93 5844
Fragments arranged by size:
5844 1369 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3109 3109
Size: 7306
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 3531 3629 3641 3941 3991 4420
Size: 399 429 1312 98 12 300 50 429
Cuts at: 4420 4625 5367 5452 6523 1391
Size: 205 742 85 1071 2174
Fragments arranged by size:
2174 1312 1071 742 429 429 399 300
205 98 85 50 12
BspEI T'CCGG_A
Cuts at: 6969 6969
Size: 7306
BspHI T'CATG_A
Cuts at: 5195 6925 5195
Size: 1730 5576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3585 4019 4661 844
Size: 2741 434 642 3489
Fragments arranged by size:
3489 2741 642 434
BsrBI CCG'CTC
Cuts at: 1394 1823 5193 1394
Size: 429 3370 3507
Fragments arranged by size:
3507 3370 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5801 5975 65
Size: 1183 4553 174 1396
Fragments arranged by size:
4553 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3041 5950 6137 1700
Size: 110 1231 2909 187 2869
Fragments arranged by size:
2909 2869 1231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7306
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7306
BssSI C'ACGA_G
Cuts at: 5366 6378 5366
Size: 1012 6294
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7306
BstBI TT'CG_AA
Cuts at: 6110 6110
Size: 7306
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7306
BstXI CCAn_nnnn'nTGG
Cuts at: 4391 4391
Size: 7306
BstYI r'GATC_y
Cuts at: 1972 2106 2223 2980 3398 4636 5003 5388 5405
Size: 134 117 757 418 1238 367 385 17
Cuts at: 5405 6857 6943 6955 7301 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1238 757 418 385 367 346
134 117 86 17 12
BstZ17I GTA'TAC
Cuts at: 2294 2294
Size: 7306
Bsu36I CC'TnA_GG
Cuts at: 2277 3543 4549 2277
Size: 1266 1006 5034
Fragments arranged by size:
5034 1266 1006
BtgI C'CryG_G
Cuts at: 513 1065 2004 2171 4122 513
Size: 552 939 167 1951 3697
Fragments arranged by size:
3697 1951 939 552 167
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6567
Fragments arranged by size:
6567 683 56
BtsI GCAGTG_nn'
Cuts at: 3531 3941 4059 4822 5616 5636 3531
Size: 410 118 763 794 20 5201
Fragments arranged by size:
5201 794 763 410 118 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2248 2283 4104 4139 529
Size: 35 1684 35 1821 35 3696
Fragments arranged by size:
3696 1821 1684 35 35 35
DraI TTT'AAA
Cuts at: 2929 3862 4610 2929
Size: 933 748 5625
Fragments arranged by size:
5625 933 748
DraIII CAC_nnn'GTG
Cuts at: 3936 3936
Size: 7306
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3466 4367 6313 809
Size: 507 2150 901 1946 1802
Fragments arranged by size:
2150 1946 1802 901 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4461 4753 5642
Size: 54 1425 50 275 18 2631 292 889
Cuts at: 5642 7271 8
Size: 1629 43
Fragments arranged by size:
2631 1629 1425 889 292 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4753 1537
Size: 275 2941 4090
Fragments arranged by size:
4090 2941 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2579 3345 3655 5236 1353
Size: 1226 766 310 1581 3423
Fragments arranged by size:
3423 1581 1226 766 310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5893 6267 6413 387
Size: 1064 138 4304 374 146 1280
Fragments arranged by size:
4304 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2772 3058 3537 3822 5369 5966
Size: 393 699 441 286 479 285 1547 597
Cuts at: 5966 6753 7021 1239
Size: 787 268 1524
Fragments arranged by size:
1547 1524 787 699 597 479 441 393
286 285 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3542 3733 3542
Size: 191 7115
EcoO109I rG'GnC_Cy
Cuts at: 3547 3868 3547
Size: 321 6985
EcoRI G'AATT_C
Cuts at: 3289 4615 4993 3289
Size: 1326 378 5602
Fragments arranged by size:
5602 1326 378
EcoRV GAT'ATC
Cuts at: 2390 2482 2390
Size: 92 7214
FspI TGC'GCA
Cuts at: 5812 5812
Size: 7306
HaeII r_GCGC'y
Cuts at: 1678 6453 1678
Size: 4775 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2544 2576 3526 3558 3906
Size: 32 135 32 972 32 950 32 348
Cuts at: 3906 3938 5947 5979 6021 6053 1373
Size: 32 2009 32 42 32 2626
Fragments arranged by size:
2626 2009 972 950 348 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2468 2844 4650 4896 669
Size: 988 281 530 376 1806 246 3079
Fragments arranged by size:
3079 1806 988 530 376 281 246
HindIII A'AGCT_T
Cuts at: 748 3745 748
Size: 2997 4309
HpaI GTT'AAC
Cuts at: 4896 4896
Size: 7306
KpnI G_GTAC'C
Cuts at: 4631 4631
Size: 7306
MfeI C'AATT_G
Cuts at: 4023 4905 4023
Size: 882 6424
MluI A'CGCG_T
Cuts at: 7182 7182
Size: 7306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6420 6604 1305
Size: 5115 184 2007
Fragments arranged by size:
5115 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5484
Fragments arranged by size:
5484 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3600 5264 5623 5782 518
Size: 648 2434 1664 359 159 2042
Fragments arranged by size:
2434 2042 1664 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3391 3800 4722 5399 6547 6792 1606
Size: 1785 409 922 677 1148 245 2120
Fragments arranged by size:
2120 1785 1148 922 677 409 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5815
Fragments arranged by size:
5815 939 552
NdeI CA'TA_TG
Cuts at: 387 2508 387
Size: 2121 5185
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7306
NotI GC'GGCC_GC
Cuts at: 4753 4753
Size: 7306
NspI r_CATG'y
Cuts at: 1132 1229 2734 3832 4664 6209 1132
Size: 97 1505 1098 832 1545 2229
Fragments arranged by size:
2229 1545 1505 1098 832 97
PasI CC'CwG_GG
Cuts at: 1348 3484 3946 4265 1348
Size: 2136 462 319 4389
Fragments arranged by size:
4389 2136 462 319
PciI A'CATG_T
Cuts at: 2730 6205 2730
Size: 3475 3831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3484 1108
Size: 2376 4930
PmeI GTTT'AAAC
Cuts at: 4610 4610
Size: 7306
PmlI CAC'GTG
Cuts at: 3161 3161
Size: 7306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5375 5407 6888 6920 4178
Size: 32 1165 32 1481 32 4564
Fragments arranged by size:
4564 1481 1165 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3868 3868
Size: 7306
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7306
PsiI TTA'TAA
Cuts at: 4876 4876
Size: 7306
PspXI vC'TCGA_Gb
Cuts at: 3833 3833
Size: 7306
PstI C_TGCA'G
Cuts at: 830 3582 4221 4658 830
Size: 2752 639 437 3478
Fragments arranged by size:
3478 2752 639 437
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7306
PvuII CAG'CTG
Cuts at: 3391 3800 3391
Size: 409 6897
SalI G'TCGA_C
Cuts at: 1936 2466 4648 1936
Size: 530 2182 4594
Fragments arranged by size:
4594 2182 530
SapI GCTCTTCn'nnn_
Cuts at: 2579 3345 2579
Size: 766 6540
SbfI CC_TGCA'GG
Cuts at: 4658 4658
Size: 7306
ScaI AGT'ACT
Cuts at: 5554 7266 5554
Size: 1712 5594
SfcI C'TryA_G
Cuts at: 826 1045 3578 4217 4654 5789 6470 6661 826
Size: 219 2533 639 437 1135 681 191 1471
Fragments arranged by size:
2533 1471 1135 681 639 437 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7306
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7306
SmaI CCC'GGG
Cuts at: 1872 4633 1872
Size: 2761 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3588 3833 3982 5410 6311 6573
Size: 197 926 1645 245 149 1428 901 262
Cuts at: 6573 820
Size: 1553
Fragments arranged by size:
1645 1553 1428 926 901 262 245 197
149
SnaBI TAC'GTA
Cuts at: 493 5097 493
Size: 4604 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7306
SphI G_CATG'C
Cuts at: 1132 3832 4664 1132
Size: 2700 832 3774
Fragments arranged by size:
3774 2700 832
SspI AAT'ATT
Cuts at: 5 52 5230 5
Size: 47 5178 2081
Fragments arranged by size:
5178 2081 47
StuI AGG'CCT
Cuts at: 1517 3621 3739 1517
Size: 2104 118 5084
Fragments arranged by size:
5084 2104 118
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2572 3616 4745 513
Size: 552 939 144 424 1044 1129 3074
Fragments arranged by size:
3074 1129 1044 939 552 424 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2739 3201 3659 4255 4456 5333 5350
Size: 1255 1159 462 458 596 201 877 17
Cuts at: 5350 5503 5688 6133 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1159 877 596 462 458 445
201 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3385 5552 7264
Size: 274 80 33 51 1443 1408 2167 1712
Cuts at: 7264 96
Size: 138
Fragments arranged by size:
2167 1712 1443 1408 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7266 7298 7266
Size: 32 7274
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7306
XbaI T'CTAG_A
Cuts at: 4642 4642
Size: 7306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 3033 2117
Size: 916 6390
XhoI C'TCGA_G
Cuts at: 1943 3833 1943
Size: 1890 5416
XmaI C'CCGG_G
Cuts at: 1870 4631 1870
Size: 2761 4545
XmnI GAAnn'nnTTC
Cuts at: 5435 5435
Size: 7306
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5114 276
Size: 53 83 186 936 3580 2468
Fragments arranged by size:
3580 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI EcoICRI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI