Restriction map for
pFN21AE2336
(Circular) (Six-base) MAPSORT of: pFN21AE2336.seq Check: 7140 from: 1 to: 8680
pFN21AE2336 8680 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6490 278
Size: 53 83 186 936 4954 2468
Fragments arranged by size:
4954 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5877 6023 1937
Size: 3940 146 4594
Fragments arranged by size:
4594 3940 146
Acc65I G'GTAC_C
Cuts at: 6001 6001
Size: 8680
AclI AA'CG_TT
Cuts at: 1563 6807 7180 1563
Size: 5244 373 3063
Fragments arranged by size:
5244 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4101 4102 5028 5198 6743 8127 8395 1632
Size: 2469 1 926 170 1545 1384 268 1917
Fragments arranged by size:
2469 1917 1545 1384 926 268 170 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8483
AflIII A'CryG_T
Cuts at: 1203 2065 7579 8556 1203
Size: 862 5514 977 1327
Fragments arranged by size:
5514 1327 977 862
AgeI A'CCGG_T
Cuts at: 7511 7511
Size: 8680
AhdI GACnn_n'nnGTC
Cuts at: 7409 7409
Size: 8680
AleI CACnn'nnGTG
Cuts at: 1166 3020 1166
Size: 1854 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2688 1184
Size: 1504 7176
AlwNI CAG_nnn'CTG
Cuts at: 2681 3814 4780 6423 7995 2681
Size: 1133 966 1643 1572 3366
Fragments arranged by size:
3366 1643 1572 1133 966
ApaLI G'TGCA_C
Cuts at: 6737 7893 6737
Size: 1156 7524
ApoI r'AATT_y
Cuts at: 1493 2292 2962 3744 4302 4464 5295 5669 5989
Size: 799 670 782 558 162 831 374 320
Cuts at: 5989 6215 6367 8476 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 799 782 670 558 374
320 226 162 152
AseI AT'TA_AT
Cuts at: 160 1032 2355 7234 160
Size: 872 1323 4879 1606
Fragments arranged by size:
4879 1606 1323 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8680
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 3460 6005 1870
Size: 73 1437 80 2545 4545
Fragments arranged by size:
4545 2545 1437 80 73
BamHI G'GATC_C
Cuts at: 5410 5525 5626 6010 6377 5410
Size: 115 101 384 367 7713
Fragments arranged by size:
7713 384 367 115 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6001 7456 618
Size: 325 217 627 4214 1455 1842
Fragments arranged by size:
4214 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4737 5999 1391
Size: 3346 1262 4072
Fragments arranged by size:
4072 3346 1262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2160 2573 3966 4135 5761 928
Size: 971 261 413 1393 169 1626 3847
Fragments arranged by size:
3847 1626 1393 971 413 261 169
BbvCI CC'TCA_GC
Cuts at: 3115 3115
Size: 8680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2977 3011 6871 6905 1903
Size: 34 50 34 956 34 3860 34 3678
Fragments arranged by size:
3860 3678 956 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5497 6572 7782 5497
Size: 1075 1210 6395
Fragments arranged by size:
6395 1210 1075
BclI T'GATC_A
Cuts at: 1548 2620 3039 1548
Size: 1072 419 7189
Fragments arranged by size:
7189 1072 419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7291 136
Size: 107 122 71 631 605 5619 1525
Fragments arranged by size:
5619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4282 4349 5310 5485 8675 4282
Size: 67 961 175 3190 4287
Fragments arranged by size:
4287 3190 961 175 67
BlpI GC'TnA_GC
Cuts at: 6097 6097
Size: 8680
Bme1580I G_kGCm'C
Cuts at: 1790 6741 7897 1790
Size: 4951 1156 2573
Fragments arranged by size:
4951 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4046 4225 5840
Size: 416 53 83 399 24 2629 179 1615
Cuts at: 5840 6411 7369 442
Size: 571 958 1753
Fragments arranged by size:
2629 1753 1615 958 571 416 399 179
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3912 4046 4712 7340 1239
Size: 2673 134 666 2628 2579
Fragments arranged by size:
2673 2628 2579 666 134
Bpu10I CC'TnA_GC
Cuts at: 3115 4176 3115
Size: 1061 7619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6805 7670 7968 6805
Size: 865 298 7517
Fragments arranged by size:
7517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3269 3990 4570 7343 882
Size: 617 1770 721 580 2773 2219
Fragments arranged by size:
2773 2219 1770 721 617 580
BsaAI yAC'GTr
Cuts at: 493 1204 3022 6471 493
Size: 711 1818 3449 2702
Fragments arranged by size:
3449 2702 1818 711
BsaBI GATnn'nnATC
Cuts at: 3573 4449 8674 3573
Size: 876 4225 3579
Fragments arranged by size:
4225 3579 876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6487 6869 7504 275
Size: 53 83 186 936 4954 382 635 1451
Fragments arranged by size:
4954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4429 5234 7113 7511 7785 7932 8343 4429
Size: 805 1879 398 274 147 411 4766
Fragments arranged by size:
4766 1879 805 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4251 4281 1178
Size: 30 165 30 2848 30 5577
Fragments arranged by size:
5577 2848 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2803 3017 3487 4082 4272 1186
Size: 1006 611 214 470 595 190 5594
Fragments arranged by size:
5594 1006 611 595 470 214 190
BseYI C'CCAG_C
Cuts at: 2489 2720 7883 8379 2489
Size: 231 5163 496 2790
Fragments arranged by size:
5163 2790 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3260 3714 3860 5665 1163
Size: 573 1524 454 146 1805 4178
Fragments arranged by size:
4178 1805 1524 573 454 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6130 6891 7919 1540
Size: 275 186 4129 761 1028 2301
Fragments arranged by size:
4129 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3027 3381 3541 4024 4132 5427 6741 6826 7897
Size: 354 160 483 108 1295 1314 85 1071
Cuts at: 7897 3027
Size: 3810
Fragments arranged by size:
3810 1314 1295 1071 483 354 160 108
85
BsiWI C'GTAC_G
Cuts at: 2406 2406
Size: 8680
BsmI GAATG_Cn'
Cuts at: 5452 6191 6284 5452
Size: 739 93 7848
Fragments arranged by size:
7848 739 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3027 3381 3541 4024 4132 4737 5427
Size: 399 1237 354 160 483 108 605 690
Cuts at: 5427 5999 6741 6826 7897 1391
Size: 572 742 85 1071 2174
Fragments arranged by size:
2174 1237 1071 742 690 605 572 483
399 354 160 108 85
BspEI T'CCGG_A
Cuts at: 8343 8343
Size: 8680
BspHI T'CATG_A
Cuts at: 6569 8299 6569
Size: 1730 6950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4927 6035 844
Size: 4083 1108 3489
Fragments arranged by size:
4083 3489 1108
BsrBI CCG'CTC
Cuts at: 1394 1823 2160 6567 1394
Size: 429 337 4407 3507
Fragments arranged by size:
4407 3507 429 337
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 5694 7175 7349 65
Size: 1183 3293 1153 1481 174 1396
Fragments arranged by size:
3293 1481 1396 1183 1153 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7324 7511 1700
Size: 110 5514 187 2869
Fragments arranged by size:
5514 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5151 96
Size: 5055 3625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8680
BssSI C'ACGA_G
Cuts at: 6740 7752 6740
Size: 1012 7668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2681 3701 794
Size: 1887 1020 5773
Fragments arranged by size:
5773 1887 1020
BstBI TT'CG_AA
Cuts at: 3726 7484 3726
Size: 3758 4922
BstEII G'GTnAC_C
Cuts at: 1184 3097 1184
Size: 1913 6767
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 5106 2341
Size: 2765 5915
BstYI r'GATC_y
Cuts at: 1972 4282 4349 5310 5410 5485 5525 5626 6010
Size: 2310 67 961 100 75 40 101 384
Cuts at: 6010 6377 6762 6779 8231 8317 8329 8675 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2310 1977 1452 961 385 384 367 346
101 100 86 75 67 40 17 12
BstZ17I GTA'TAC
Cuts at: 5878 5878
Size: 8680
Bsu36I CC'TnA_GG
Cuts at: 2951 5367 2951
Size: 2416 6264
BtgI C'CryG_G
Cuts at: 513 1065 2379 2418 2559 3342 513
Size: 552 1314 39 141 783 5851
Fragments arranged by size:
5851 1314 783 552 141 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7941
Fragments arranged by size:
7941 683 56
BtsI GCAGTG_nn'
Cuts at: 2660 2682 3040 3072 3654 4167 5677 6196 6990
Size: 22 358 32 582 513 1510 519 794
Cuts at: 6990 7010 2660
Size: 20 4330
Fragments arranged by size:
4330 1510 794 582 519 513 358 32
22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4184 4219 529
Size: 35 3620 35 4990
Fragments arranged by size:
4990 3620 35 35
DraI TTT'AAA
Cuts at: 2098 3897 4470 5984 2098
Size: 1799 573 1514 4794
Fragments arranged by size:
4794 1799 1514 573
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7687 809
Size: 507 6371 1802
Fragments arranged by size:
6371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2556 4578
Size: 54 1425 50 275 18 274 452 2022
Cuts at: 4578 6127 7016 8645 8
Size: 1549 889 1629 43
Fragments arranged by size:
2022 1629 1549 1425 889 452 275 274
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6127 1537
Size: 275 4315 4090
Fragments arranged by size:
4315 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3110 4387 4758 6610 1353
Size: 1757 1277 371 1852 3423
Fragments arranged by size:
3423 1852 1757 1277 371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7267 7641 7787 387
Size: 1064 138 5678 374 146 1280
Fragments arranged by size:
5678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3912 4046 4101 4102 4712 5028 5198
Size: 393 2280 134 55 1 610 316 170
Cuts at: 5198 6743 7340 8127 8395 1239
Size: 1545 597 787 268 1524
Fragments arranged by size:
2280 1545 1524 787 610 597 393 316
268 170 134 55 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2950 2174
Size: 776 7904
EcoO109I rG'GnC_Cy
Cuts at: 4902 4902
Size: 8680
EcoRI G'AATT_C
Cuts at: 5989 6367 5989
Size: 378 8302
EcoRV GAT'ATC
Cuts at: 3189 3189
Size: 8680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4132 4132
Size: 8680
FspI TGC'GCA
Cuts at: 7186 7186
Size: 8680
HaeII r_GCGC'y
Cuts at: 1678 4678 7827 1678
Size: 3000 3149 2531
Fragments arranged by size:
3149 3000 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2575 2607 3116 3148 3173
Size: 32 135 32 1003 32 509 32 25
Cuts at: 3173 3205 3254 3268 3286 3300 3577 3609 3872
Size: 32 49 14 18 14 277 32 263
Cuts at: 3872 3904 5112 5144 7321 7353 7395 7427 1373
Size: 32 1208 32 2177 32 42 32 2626
Fragments arranged by size:
2626 2177 1208 1003 509 277 263 135
49 42 32 32 32 32 32 32
32 32 32 32 25 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4378 6024 6270 669
Size: 988 281 2440 1646 246 3079
Fragments arranged by size:
3079 2440 1646 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8680
HpaI GTT'AAC
Cuts at: 4378 6270 4378
Size: 1892 6788
KpnI G_GTAC'C
Cuts at: 6005 6005
Size: 8680
MfeI C'AATT_G
Cuts at: 6279 6279
Size: 8680
MluI A'CGCG_T
Cuts at: 2065 8556 2065
Size: 6491 2189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3543 5521 7794 7978 1305
Size: 2238 1978 2273 184 2007
Fragments arranged by size:
2273 2238 2007 1978 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 2558 10
Size: 54 1425 343 274 452 6132
Fragments arranged by size:
6132 1425 452 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 3020 4500 4970 4976 5430 6638
Size: 648 1392 462 1480 470 6 454 1208
Cuts at: 6638 6997 7156 518
Size: 359 159 2042
Fragments arranged by size:
2042 1480 1392 1208 648 470 462 454
359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2041 3208 3559 4516 4842 5849 6096 6773
Size: 435 1167 351 957 326 1007 247 677
Cuts at: 6773 7921 8166 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1167 1148 1007 957 677 435 351
326 247 245
NcoI C'CATG_G
Cuts at: 513 1065 2379 2559 513
Size: 552 1314 180 6634
Fragments arranged by size:
6634 1314 552 180
NdeI CA'TA_TG
Cuts at: 387 2520 387
Size: 2133 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8680
NotI GC'GGCC_GC
Cuts at: 6127 6127
Size: 8680
NspI r_CATG'y
Cuts at: 1132 1229 3239 6038 7583 1132
Size: 97 2010 2799 1545 2229
Fragments arranged by size:
2799 2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3450 1348
Size: 2102 6578
PciI A'CATG_T
Cuts at: 7579 7579
Size: 8680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5903 1108
Size: 4795 3885
PfoI T'CCnGG_A
Cuts at: 3675 3934 5661 3675
Size: 259 1727 6694
Fragments arranged by size:
6694 1727 259
PmeI GTTT'AAAC
Cuts at: 5984 5984
Size: 8680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2077 4802 4834 6749 6781 8262 8294 2045
Size: 32 2725 32 1915 32 1481 32 2431
Fragments arranged by size:
2725 2431 1915 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4902 4902
Size: 8680
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8680
PsiI TTA'TAA
Cuts at: 2843 6250 2843
Size: 3407 5273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2390 2422 4314 4346 5135 5167 2390
Size: 32 1892 32 789 32 5903
Fragments arranged by size:
5903 1892 789 32 32 32
PstI C_TGCA'G
Cuts at: 830 2046 2667 4174 6032 830
Size: 1216 621 1507 1858 3478
Fragments arranged by size:
3478 1858 1507 1216 621
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8680
PvuII CAG'CTG
Cuts at: 3208 3559 3208
Size: 351 8329
SalI G'TCGA_C
Cuts at: 1936 6022 1936
Size: 4086 4594
SanDI GG'GwC_CC
Cuts at: 4902 4902
Size: 8680
SbfI CC_TGCA'GG
Cuts at: 6032 6032
Size: 8680
ScaI AGT'ACT
Cuts at: 6928 8640 6928
Size: 1712 6968
SfcI C'TryA_G
Cuts at: 826 1045 2042 2663 4170 4320 6028 7163 7844
Size: 219 997 621 1507 150 1708 1135 681
Cuts at: 7844 8035 826
Size: 191 1471
Fragments arranged by size:
1708 1507 1471 1135 997 681 621 219
191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8680
SmaI CCC'GGG
Cuts at: 1872 6007 1872
Size: 4135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6784 7685 7947 820
Size: 197 926 4841 901 262 1553
Fragments arranged by size:
4841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6471 493
Size: 5978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8680
SphI G_CATG'C
Cuts at: 1132 6038 1132
Size: 4906 3774
SspI AAT'ATT
Cuts at: 5 52 3481 6604 5
Size: 47 3429 3123 2081
Fragments arranged by size:
3429 3123 2081 47
StuI AGG'CCT
Cuts at: 1517 3294 4103 1517
Size: 1777 809 6094
Fragments arranged by size:
6094 1777 809
StyI C'CwwG_G
Cuts at: 513 1065 2170 2379 2559 4589 4605 4615 5075
Size: 552 1105 209 180 2030 16 10 460
Cuts at: 5075 6119 513
Size: 1044 3074
Fragments arranged by size:
3074 2030 1105 1044 552 460 209 180
16 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3442 6707 6724 6877 7062 7507 325
Size: 1255 1862 3265 17 153 185 445 1498
Fragments arranged by size:
3265 1862 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5151 6926 8638
Size: 274 80 33 51 1443 3174 1775 1712
Cuts at: 8638 96
Size: 138
Fragments arranged by size:
3174 1775 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8640 8672 8640
Size: 32 8648
Tth111I GACn'n_nGTC
Cuts at: 1316 4834 1316
Size: 3518 5162
XbaI T'CTAG_A
Cuts at: 3998 6016 3998
Size: 2018 6662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 5103 2386
Size: 2717 5963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8680
XmaI C'CCGG_G
Cuts at: 1870 6005 1870
Size: 4135 4545
XmnI GAAnn'nnTTC
Cuts at: 2774 4115 4306 5248 5487 6809 2774
Size: 1341 191 942 239 1322 4645
Fragments arranged by size:
4645 1341 1322 942 239 191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6488 276
Size: 53 83 186 936 4954 2468
Fragments arranged by size:
4954 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI