Restriction map for
pFN21AE2331
(Circular) (Six-base) MAPSORT of: pFN21AE2331.seq Check: 5278 from: 1 to: 6886
pFN21AE2331 6886 bp
With 182 enzymes: SgfI *
March 30, 2011 18:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2706 3439 2706
Size: 733 6153
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 4185 5013 5386 1563
Size: 2622 828 373 3063
Fragments arranged by size:
3063 2622 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 2780 3447 4949 6333 6601 1632
Size: 704 444 667 1502 1384 268 1917
Fragments arranged by size:
1917 1502 1384 704 667 444 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 2836 5785 6762 1203
Size: 1633 2949 977 1327
Fragments arranged by size:
2949 1633 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 2467 5615 2467
Size: 3148 3738
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6886
AlwNI CAG_nnn'CTG
Cuts at: 3391 3529 4629 6201 3391
Size: 138 1100 1572 4076
Fragments arranged by size:
4076 1572 1100 138
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 4195 4421 4573 6682 1493
Size: 2702 226 152 2109 1697
Fragments arranged by size:
2702 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2745 3090 5440 160
Size: 872 1713 345 2350 1606
Fragments arranged by size:
2350 1713 1606 872 345
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 2337 4022 4211 1870
Size: 73 344 50 1685 189 4545
Fragments arranged by size:
4545 1685 344 189 73 50
BamHI G'GATC_C
Cuts at: 4216 4583 4216
Size: 367 6519
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 3890 4207 5662 618
Size: 325 217 627 264 1839 317 1455 1842
Fragments arranged by size:
1842 1839 1455 627 325 317 264 217
BanII G_rGCy'C
Cuts at: 1391 2851 4155 4205 1391
Size: 1460 1304 50 4072
Fragments arranged by size:
4072 1460 1304 50
BbeI G_GCGC'C
Cuts at: 2055 2055
Size: 6886
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2928 3228 3435 928
Size: 971 1029 300 207 4379
Fragments arranged by size:
4379 1029 971 300 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5077 5111 1903
Size: 34 50 34 3056 34 3678
Fragments arranged by size:
3678 3056 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2518 3392 4778 5988 2518
Size: 874 1386 1210 3416
Fragments arranged by size:
3416 1386 1210 874
BclI T'GATC_A
Cuts at: 1548 2077 3645 1548
Size: 529 1568 4789
Fragments arranged by size:
4789 1568 529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2855 5497 136
Size: 107 122 71 631 605 1183 2642 1525
Fragments arranged by size:
2642 1525 1183 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3282 6881 3282
Size: 3599 3287
BlpI GC'TnA_GC
Cuts at: 4073 4303 4073
Size: 230 6656
Bme1580I G_kGCm'C
Cuts at: 1790 3804 3895 4947 6103 1790
Size: 2014 91 1052 1156 2573
Fragments arranged by size:
2573 2014 1156 1052 91
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4617 5575 442
Size: 416 53 83 399 24 3200 958 1753
Fragments arranged by size:
3200 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2229 5546 1239
Size: 902 88 3317 2579
Fragments arranged by size:
3317 2579 902 88
Bpu10I CC'TnA_GC
Cuts at: 3530 3530
Size: 6886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 5011 5876 6174 3224
Size: 1787 865 298 3936
Fragments arranged by size:
3936 1787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2133 3305 5549 882
Size: 617 634 1172 2244 2219
Fragments arranged by size:
2244 2219 1172 634 617
BsaAI yAC'GTr
Cuts at: 493 1204 4677 493
Size: 711 3473 2702
Fragments arranged by size:
3473 2702 711
BsaBI GATnn'nnATC
Cuts at: 2675 6880 2675
Size: 4205 2681
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 4693 5075 5710
Size: 53 83 186 936 519 2641 382 635
Cuts at: 5710 275
Size: 1451
Fragments arranged by size:
2641 1451 936 635 519 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3192 5319 5717 5991 6138 6549 3192
Size: 2127 398 274 147 411 3529
Fragments arranged by size:
3529 2127 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2227 2257 3109 3139 1178
Size: 30 165 30 824 30 852 30 4925
Fragments arranged by size:
4925 852 824 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 2791 3377 1186
Size: 1192 413 586 4695
Fragments arranged by size:
4695 1192 586 413
BseYI C'CCAG_C
Cuts at: 2259 2850 4154 6089 6585 2259
Size: 591 1304 1935 496 2560
Fragments arranged by size:
2560 1935 1304 591 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6313
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 2904 3478 4947 5032 6103 2634
Size: 270 574 1469 85 1071 3417
Fragments arranged by size:
3417 1469 1071 574 270 85
BsmI GAATG_Cn'
Cuts at: 4018 4397 4490 4018
Size: 379 93 6414
Fragments arranged by size:
6414 379 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2634 2851 2904 3478 3804 3895 4155
Size: 399 844 217 53 574 326 91 260
Cuts at: 4155 4205 4947 5032 6103 1391
Size: 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 574 399 326 260
217 91 85 53 50
BspEI T'CCGG_A
Cuts at: 3192 6549 3192
Size: 3357 3529
BspHI T'CATG_A
Cuts at: 2442 4775 6505 2442
Size: 2333 1730 2823
Fragments arranged by size:
2823 2333 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 3323 3439 3454 3692 4241 844
Size: 1862 617 116 15 238 549 3489
Fragments arranged by size:
3489 1862 617 549 238 116 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2409 4773 1394
Size: 429 586 2364 3507
Fragments arranged by size:
3507 2364 586 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2209 5381 5555 65
Size: 1183 961 3172 174 1396
Fragments arranged by size:
3172 1396 1183 961 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3829 5530 5717 1700
Size: 110 2019 1701 187 2869
Fragments arranged by size:
2869 2019 1701 187 110
BsrGI T'GTAC_A
Cuts at: 96 3057 96
Size: 2961 3925
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 4946 5958 4946
Size: 1012 5874
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6886
BstBI TT'CG_AA
Cuts at: 2986 5690 2986
Size: 2704 4182
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2068
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 3282 3353 3543 4119 4216 4583 4968 4985
Size: 1310 71 190 576 97 367 385 17
Cuts at: 4985 6437 6523 6535 6881 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1310 576 385 367 346 190
97 86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3789 3789
Size: 6886
BtgI C'CryG_G
Cuts at: 513 1065 2016 2506 3108 3849 513
Size: 552 951 490 602 741 3550
Fragments arranged by size:
3550 951 741 602 552 490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3952 487
Size: 683 56 2726 3421
Fragments arranged by size:
3421 2726 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 3034 4402 5196 5216 2745
Size: 289 1368 794 20 4415
Fragments arranged by size:
4415 1368 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3010 3045 529
Size: 35 2446 35 4370
Fragments arranged by size:
4370 2446 35 35
DraI TTT'AAA
Cuts at: 4190 4190
Size: 6886
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2045 2325
Size: 54 1425 50 275 18 204 11 280
Cuts at: 2325 2610 2680 3244 4333 5222 6851 8
Size: 285 70 564 1089 889 1629 43
Fragments arranged by size:
1629 1425 1089 889 564 285 280 275
204 70 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3774 4816 1353
Size: 2421 1042 3423
Fragments arranged by size:
3423 2421 1042
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3545 3724 4082 4125 5473 5847
Size: 1064 138 1956 179 358 43 1348 374
Cuts at: 5847 5993 387
Size: 146 1280
Fragments arranged by size:
1956 1348 1280 1064 374 358 179 146
138 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2229 2336 2780 3447 4949 5546
Size: 393 509 88 107 444 667 1502 597
Cuts at: 5546 6333 6601 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1502 787 667 597 509 444 393
268 107 88
EcoNI CCTnn'n_nnAGG
Cuts at: 3688 3688
Size: 6886
EcoO109I rG'GnC_Cy
Cuts at: 3681 3681
Size: 6886
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
EcoRV GAT'ATC
Cuts at: 3841 3841
Size: 6886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 3012 3911 2470
Size: 542 899 5445
Fragments arranged by size:
5445 899 542
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 2055 6033 1678
Size: 377 3978 2531
Fragments arranged by size:
3978 2531 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2366 2398 2453
Size: 32 135 32 539 32 223 32 55
Cuts at: 2453 2485 2993 3025 3127 3159 3575 3607 5527
Size: 32 508 32 102 32 416 32 1920
Cuts at: 5527 5559 5601 5633 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1920 539 508 416 223 135 102
55 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3100 4230 4476 669
Size: 988 281 1162 1130 246 3079
Fragments arranged by size:
3079 1162 1130 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3077 748
Size: 2329 4557
HpaI GTT'AAC
Cuts at: 4476 4476
Size: 6886
KasI G'GCGC_C
Cuts at: 2051 2051
Size: 6886
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 4485 4485
Size: 6886
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6000 6184 1305
Size: 4695 184 2007
Fragments arranged by size:
4695 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2327 2612 10
Size: 54 1425 343 204 291 285 4284
Fragments arranged by size:
4284 1425 343 291 285 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 3180 4844 5203 5362 518
Size: 648 1230 784 1664 359 159 2042
Fragments arranged by size:
2042 1664 1230 784 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2602 2854 2884 4302 4979 6127 6372
Size: 412 584 252 30 1418 677 1148 245
Cuts at: 6372 1606
Size: 2120
Fragments arranged by size:
2120 1418 1148 677 584 412 252 245
30
NarI GG'CG_CC
Cuts at: 2052 2052
Size: 6886
NcoI C'CATG_G
Cuts at: 513 1065 2506 3108 513
Size: 552 1441 602 4291
Fragments arranged by size:
4291 1441 602 552
NdeI CA'TA_TG
Cuts at: 387 3876 387
Size: 3489 3397
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2165 2467 2840 3157 4244 5789 1132
Size: 97 936 302 373 317 1087 1545 2229
Fragments arranged by size:
2229 1545 1087 936 373 317 302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 2836 5785 2836
Size: 2949 3937
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2043 2659 1108
Size: 935 616 5335
Fragments arranged by size:
5335 935 616
PfoI T'CCnGG_A
Cuts at: 2916 4098 2916
Size: 1182 5704
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4955 4987 6468 6500 4955
Size: 32 1481 32 5341
Fragments arranged by size:
5341 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3681 3681
Size: 6886
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 2366 3072 4456 2366
Size: 706 1384 4796
Fragments arranged by size:
4796 1384 706
PspXI vC'TCGA_Gb
Cuts at: 2287 2287
Size: 6886
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 6854
PstI C_TGCA'G
Cuts at: 830 2601 3525 4238 830
Size: 1771 924 713 3478
Fragments arranged by size:
3478 1771 924 713
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 2884 2884
Size: 6886
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 6886
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 3989 5134 6846 3989
Size: 1145 1712 4029
Fragments arranged by size:
4029 1712 1145
SfcI C'TryA_G
Cuts at: 826 1045 2597 3521 4234 5369 6050 6241 826
Size: 219 1552 924 713 1135 681 191 1471
Fragments arranged by size:
1552 1471 1135 924 713 681 219 191
SfoI GGC'GCC
Cuts at: 2053 2053
Size: 6886
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 2339 4213 1872
Size: 467 1874 4545
Fragments arranged by size:
4545 1874 467
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2287 3203 4990 5891 6153 820
Size: 197 926 344 916 1787 901 262 1553
Fragments arranged by size:
1787 1553 926 916 901 344 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 2165 4244 1132
Size: 1033 2079 3774
Fragments arranged by size:
3774 2079 1033
SspI AAT'ATT
Cuts at: 5 52 2147 2797 4810 5
Size: 47 2095 650 2013 2081
Fragments arranged by size:
2095 2081 2013 650 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6886
StyI C'CwwG_G
Cuts at: 513 1065 2506 2607 3108 4146 4325 513
Size: 552 1441 101 501 1038 179 3074
Fragments arranged by size:
3074 1441 1038 552 501 179 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2112 3359 4913 4930 5083 5268 5713
Size: 1255 532 1247 1554 17 153 185 445
Cuts at: 5713 325
Size: 1498
Fragments arranged by size:
1554 1498 1255 1247 532 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3057 3159 3987
Size: 274 80 33 51 1443 1080 102 828
Cuts at: 3987 5132 6844 96
Size: 1145 1712 138
Fragments arranged by size:
1712 1443 1145 1080 828 274 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2826 2858 3109 3141 3338 3370 6846 6878 2826
Size: 32 251 32 197 32 3476 32 2834
Fragments arranged by size:
3476 2834 251 197 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2024 4169 1316
Size: 708 2145 4033
Fragments arranged by size:
4033 2145 708
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XhoI C'TCGA_G
Cuts at: 1943 2287 1943
Size: 344 6542
XmaI C'CCGG_G
Cuts at: 1870 2337 4211 1870
Size: 467 1874 4545
Fragments arranged by size:
4545 1874 467
XmnI GAAnn'nnTTC
Cuts at: 5015 5015
Size: 6886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI