Restriction map for
pFN21AE2329
(Circular) (Six-base) MAPSORT of: pFN21AE2329.seq Check: 8273 from: 1 to: 8644
pFN21AE2329 8644 bp
With 182 enzymes: SgfI *
February 23, 2011 17:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3122 5632 3122
Size: 2510 6134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6454 278
Size: 53 83 186 936 4918 2468
Fragments arranged by size:
4918 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 3242 3269 5714 5987 1937
Size: 831 474 27 2445 273 4594
Fragments arranged by size:
4594 2445 831 474 273 27
Acc65I G'GTAC_C
Cuts at: 5965 5965
Size: 8644
AclI AA'CG_TT
Cuts at: 1563 6771 7144 1563
Size: 5208 373 3063
Fragments arranged by size:
5208 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 3585 3837 4176 5259 5436 5898 6707
Size: 881 1072 252 339 1083 177 462 809
Cuts at: 6707 8091 8359 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1083 1072 881 809 462 339
268 252 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8447
AflIII A'CryG_T
Cuts at: 1203 7543 8520 1203
Size: 6340 977 1327
Fragments arranged by size:
6340 1327 977
AgeI A'CCGG_T
Cuts at: 7475 7475
Size: 8644
AhdI GACnn_n'nnGTC
Cuts at: 4640 7373 4640
Size: 2733 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5136 5325 1184
Size: 3952 189 4503
Fragments arranged by size:
4503 3952 189
AlwNI CAG_nnn'CTG
Cuts at: 3824 4557 6387 7959 3824
Size: 733 1830 1572 4509
Fragments arranged by size:
4509 1830 1572 733
ApaI G_GGCC'C
Cuts at: 2478 5078 2478
Size: 2600 6044
ApaLI G'TGCA_C
Cuts at: 5856 6701 7857 5856
Size: 845 1156 6643
Fragments arranged by size:
6643 1156 845
ApoI r'AATT_y
Cuts at: 1493 3302 3433 3538 3616 3947 4400 5953 6179
Size: 1809 131 105 78 331 453 1553 226
Cuts at: 6179 6331 8440 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1809 1697 1553 453 331 226 152
131 105 78
AscI GG'CGCG_CC
Cuts at: 2426 2426
Size: 8644
AseI AT'TA_AT
Cuts at: 160 1032 7198 160
Size: 872 6166 1606
Fragments arranged by size:
6166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8644
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2143 2344 3372 3732 4009 5969
Size: 73 138 62 201 1028 360 277 1960
Cuts at: 5969 1870
Size: 4545
Fragments arranged by size:
4545 1960 1028 360 277 201 138 73
62
BamHI G'GATC_C
Cuts at: 3193 5974 6341 3193
Size: 2781 367 5496
Fragments arranged by size:
5496 2781 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2348 2387 5236 5965 7420
Size: 325 217 627 561 39 2849 729 1455
Cuts at: 7420 618
Size: 1842
Fragments arranged by size:
2849 1842 1455 729 627 561 325 217
39
BanII G_rGCy'C
Cuts at: 1391 2210 2478 3123 3572 4931 5078 5296 5387
Size: 819 268 645 449 1359 147 218 91
Cuts at: 5387 5963 1391
Size: 576 4072
Fragments arranged by size:
4072 1359 819 645 576 449 268 218
147 91
BbeI G_GCGC'C
Cuts at: 2352 2391 2352
Size: 39 8605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3843 928
Size: 971 1944 5729
Fragments arranged by size:
5729 1944 971
BbvCI CC'TCA_GC
Cuts at: 5133 5445 5133
Size: 312 8332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6835 6869 1903
Size: 34 50 34 4814 34 3678
Fragments arranged by size:
4814 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2163 3964 4923 6536 7746 2163
Size: 1801 959 1613 1210 3061
Fragments arranged by size:
3061 1801 1613 1210 959
BclI T'GATC_A
Cuts at: 1548 4497 1548
Size: 2949 5695
BfrBI ATG'CAT
Cuts at: 5321 5321
Size: 8644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2086 2110 2357
Size: 107 122 71 631 605 414 24 247
Cuts at: 2357 3763 5543 7255 136
Size: 1406 1780 1712 1525
Fragments arranged by size:
1780 1712 1525 1406 631 605 414 247
122 107 71 24
BglII A'GATC_T
Cuts at: 8639 8639
Size: 8644
BlpI GC'TnA_GC
Cuts at: 2011 4722 5394 6061 2011
Size: 2711 672 667 4594
Fragments arranged by size:
4594 2711 672 667
Bme1580I G_kGCm'C
Cuts at: 1790 2478 3279 3361 3456 5078 5860 6705 7861
Size: 688 801 82 95 1622 782 845 1156
Cuts at: 7861 1790
Size: 2573
Fragments arranged by size:
2573 1622 1156 845 801 782 688 95
82
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3355 3963 4104
Size: 416 53 83 399 24 1938 608 141
Cuts at: 4104 4916 5502 6375 7333 442
Size: 812 586 873 958 1753
Fragments arranged by size:
1938 1753 958 873 812 608 586 416
399 141 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8644
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2229 2229
Size: 8644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 3022 3137 3745 3801 3972 4854 7304
Size: 842 941 115 608 56 171 882 2450
Cuts at: 7304 1239
Size: 2579
Fragments arranged by size:
2579 2450 941 882 842 608 171 115
56
Bpu10I CC'TnA_GC
Cuts at: 4524 5133 5445 4524
Size: 609 312 7723
Fragments arranged by size:
7723 609 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3179 4809 6769 7634 7932 3179
Size: 1630 1960 865 298 3891
Fragments arranged by size:
3891 1960 1630 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2297 2665 4083 7307 882
Size: 617 798 368 1418 3224 2219
Fragments arranged by size:
3224 2219 1418 798 617 368
BsaAI yAC'GTr
Cuts at: 493 1204 3246 5740 6435 493
Size: 711 2042 2494 695 2702
Fragments arranged by size:
2702 2494 2042 711 695
BsaBI GATnn'nnATC
Cuts at: 8638 8638
Size: 8644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 2388 2415 4964
Size: 53 83 186 936 816 39 27 2549
Cuts at: 4964 6451 6833 7468 275
Size: 1487 382 635 1451
Fragments arranged by size:
2549 1487 1451 936 816 635 382 186
83 53 39 27
BsaWI w'CCGG_w
Cuts at: 7077 7475 7749 7896 8307 7077
Size: 398 274 147 411 7414
Fragments arranged by size:
7414 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8419
Fragments arranged by size:
8419 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2016 2653 2677 3975 1186
Size: 830 637 24 1298 5855
Fragments arranged by size:
5855 1298 830 637 24
BseYI C'CCAG_C
Cuts at: 2499 3885 3917 4589 4672 5059 5253 5659 7847
Size: 1386 32 672 83 387 194 406 2188
Cuts at: 7847 8343 2499
Size: 496 2800
Fragments arranged by size:
2800 2188 1386 672 496 406 387 194
83 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5408 5487 1163
Size: 573 3672 79 4320
Fragments arranged by size:
4320 3672 573 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2105 2361 3755 6094 6855
Size: 275 186 18 86 256 1394 2339 761
Cuts at: 6855 7883 1540
Size: 1028 2301
Fragments arranged by size:
2339 2301 1394 1028 761 275 256 186
86 18
BsiHKAI G_wGCw'C
Cuts at: 2210 4626 5860 6705 6790 7861 2210
Size: 2416 1234 845 85 1071 2993
Fragments arranged by size:
2993 2416 1234 1071 845 85
BsmI GAATG_Cn'
Cuts at: 6155 6248 6155
Size: 93 8551
BsmBI CGTCTCn'nnnn_
Cuts at: 2422 3798 2422
Size: 1376 7268
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2478 3123 3279 3361 3456 3572
Size: 399 420 268 645 156 82 95 116
Cuts at: 3572 4626 4931 5078 5296 5387 5860 5963 6705
Size: 1054 305 147 218 91 473 103 742
Cuts at: 6705 6790 7861 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1054 742 645 473 420 399
305 268 218 156 147 116 103 95
91 85 82
BspEI T'CCGG_A
Cuts at: 8307 8307
Size: 8644
BspHI T'CATG_A
Cuts at: 5296 6533 8263 5296
Size: 1237 1730 5677
Fragments arranged by size:
5677 1730 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 5632 5999 844
Size: 2278 2510 367 3489
Fragments arranged by size:
3489 2510 2278 367
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 2383 6531 1394
Size: 429 295 265 4148 3507
Fragments arranged by size:
4148 3507 429 295 265
BsrDI GCAATG_nn'
Cuts at: 65 1248 7139 7313 65
Size: 1183 5891 174 1396
Fragments arranged by size:
5891 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2104 2464 5707 7288 7475 1700
Size: 110 294 360 3243 1581 187 2869
Fragments arranged by size:
3243 2869 1581 360 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 5755 96
Size: 5659 2985
BssHII G'CGCG_C
Cuts at: 1925 2426 1925
Size: 501 8143
BssSI C'ACGA_G
Cuts at: 6704 7716 6704
Size: 1012 7632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8644
BstBI TT'CG_AA
Cuts at: 7448 7448
Size: 8644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8644
BstXI CCAn_nnnn'nTGG
Cuts at: 5489 5489
Size: 8644
BstYI r'GATC_y
Cuts at: 1972 3193 3584 5974 6341 6726 6743 8195 8281
Size: 1221 391 2390 367 385 17 1452 86
Cuts at: 8281 8293 8639 1972
Size: 12 346 1977
Fragments arranged by size:
2390 1977 1452 1221 391 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3270 5715 3270
Size: 2445 6199
Bsu36I CC'TnA_GG
Cuts at: 4638 5745 4638
Size: 1107 7537
BtgI C'CryG_G
Cuts at: 513 1065 2037 2577 2676 5379 513
Size: 552 972 540 99 2703 3778
Fragments arranged by size:
3778 2703 972 552 540 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3648 5327 487
Size: 683 56 2422 1679 3804
Fragments arranged by size:
3804 2422 1679 683 56
BtsI GCAGTG_nn'
Cuts at: 6160 6954 6974 6160
Size: 794 20 7830
Fragments arranged by size:
7830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8609
DraI TTT'AAA
Cuts at: 2887 3025 3309 4395 5948 2887
Size: 138 284 1086 1553 5583
Fragments arranged by size:
5583 1553 1086 284 138
DraIII CAC_nnn'GTG
Cuts at: 3971 3971
Size: 8644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7651 809
Size: 507 6335 1802
Fragments arranged by size:
6335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2043 2102
Size: 54 1425 50 275 18 186 27 59
Cuts at: 2102 2358 2673 3659 3752 3776 5556 6091 6980
Size: 256 315 986 93 24 1780 535 889
Cuts at: 6980 8609 8
Size: 1629 43
Fragments arranged by size:
1780 1629 1425 986 889 535 315 275
256 186 93 59 54 50 43 27
24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2102 2358 3752 6091 1537
Size: 275 204 86 256 1394 2339 4090
Fragments arranged by size:
4090 2339 1394 275 256 204 86
EarI CTCTTCn'nnn_
Cuts at: 1353 3729 4698 6574 1353
Size: 2376 969 1876 3423
Fragments arranged by size:
3423 2376 1876 969
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2128 2149 2209 7231 7605 7751
Size: 1064 138 539 21 60 5022 374 146
Cuts at: 7751 387
Size: 1280
Fragments arranged by size:
5022 1280 1064 539 374 146 138 60
21
EcoICRI GAG'CTC
Cuts at: 2208 2208
Size: 8644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2513 3022 3137 3585 3745 3801
Size: 393 449 432 509 115 448 160 56
Cuts at: 3801 3837 3972 4176 4854 5259 5436 5898 6707
Size: 36 135 204 678 405 177 462 809
Cuts at: 6707 7304 8091 8359 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 809 787 678 597 509 462 449
448 432 405 393 268 204 177 160
135 115 56 36
EcoO109I rG'GnC_Cy
Cuts at: 2475 3112 3713 3765 3860 4634 2475
Size: 637 601 52 95 774 6485
Fragments arranged by size:
6485 774 637 601 95 52
EcoRI G'AATT_C
Cuts at: 3538 3616 5953 6331 3538
Size: 78 2337 378 5851
Fragments arranged by size:
5851 2337 378 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3075 3606 4452 5376 3075
Size: 531 846 924 6343
Fragments arranged by size:
6343 924 846 531
FspI TGC'GCA
Cuts at: 5406 7150 5406
Size: 1744 6900
FspAI rTGC'GCAy
Cuts at: 5406 5406
Size: 8644
HaeII r_GCGC'y
Cuts at: 1678 2092 2352 2391 7791 1678
Size: 414 260 39 5400 2531
Fragments arranged by size:
5400 2531 414 260 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3020 3052 4482 4514 4626
Size: 32 135 32 1448 32 1430 32 112
Cuts at: 4626 4658 7285 7317 7359 7391 1373
Size: 32 2627 32 42 32 2626
Fragments arranged by size:
2627 2626 1448 1430 135 112 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5988 6234 669
Size: 988 281 4050 246 3079
Fragments arranged by size:
4050 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8644
HpaI GTT'AAC
Cuts at: 6234 6234
Size: 8644
KasI G'GCGC_C
Cuts at: 2348 2387 2348
Size: 39 8605
KpnI G_GTAC'C
Cuts at: 5969 5969
Size: 8644
MfeI C'AATT_G
Cuts at: 6243 6243
Size: 8644
MluI A'CGCG_T
Cuts at: 8520 8520
Size: 8644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4175 5655 5807 7758 7942 1305
Size: 2870 1480 152 1951 184 2007
Fragments arranged by size:
2870 2007 1951 1480 184 152
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 3661 5558 10
Size: 54 1425 343 213 1616 1897 3096
Fragments arranged by size:
3096 1897 1616 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4973 5043 5193 6602 6961 7120 518
Size: 648 3807 70 150 1409 359 159 2042
Fragments arranged by size:
3807 2042 1409 648 359 159 150 70
MspA1I CmG'CkG
Cuts at: 1606 2015 2039 2101 2579 2610 4181 4721 5023
Size: 409 24 62 478 31 1571 540 302
Cuts at: 5023 5072 6060 6737 7885 8130 1606
Size: 49 988 677 1148 245 2120
Fragments arranged by size:
2120 1571 1148 988 677 540 478 409
302 245 62 49 31 24
NaeI GCC'GGC
Cuts at: 2106 5709 2106
Size: 3603 5041
NarI GG'CG_CC
Cuts at: 2349 2388 2349
Size: 39 8605
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8092
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8644
NgoMIV G'CCGG_C
Cuts at: 2104 5707 2104
Size: 3603 5041
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8644
NotI GC'GGCC_GC
Cuts at: 2016 6091 2016
Size: 4075 4569
NsiI A_TGCA'T
Cuts at: 5323 5323
Size: 8644
NspI r_CATG'y
Cuts at: 1132 1229 3850 4680 5168 5312 5325 6002 7547
Size: 97 2621 830 488 144 13 677 1545
Cuts at: 7547 1132
Size: 2229
Fragments arranged by size:
2621 2229 1545 830 677 488 144 97
13
PasI CC'CwG_GG
Cuts at: 1348 3123 1348
Size: 1775 6869
PciI A'CATG_T
Cuts at: 7543 7543
Size: 8644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8644
PfoI T'CCnGG_A
Cuts at: 3490 3490
Size: 8644
PmeI GTTT'AAAC
Cuts at: 5948 5948
Size: 8644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6713 6745 8226 8258 6713
Size: 32 1481 32 7099
Fragments arranged by size:
7099 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3112 3713 4634 3112
Size: 601 921 7122
Fragments arranged by size:
7122 921 601
PshAI GACnn'nnGTC
Cuts at: 1613 3790 4075 1613
Size: 2177 285 6182
Fragments arranged by size:
6182 2177 285
PsiI TTA'TAA
Cuts at: 6214 6214
Size: 8644
PspOMI G'GGCC_C
Cuts at: 2474 5074 2474
Size: 2600 6044
PspXI vC'TCGA_Gb
Cuts at: 3372 3372
Size: 8644
PstI C_TGCA'G
Cuts at: 830 4285 5996 830
Size: 3455 1711 3478
Fragments arranged by size:
3478 3455 1711
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8644
PvuII CAG'CTG
Cuts at: 4181 4721 4181
Size: 540 8104
SacI G_AGCT'C
Cuts at: 2210 2210
Size: 8644
SacII CC_GC'GG
Cuts at: 2040 2580 2040
Size: 540 8104
SalI G'TCGA_C
Cuts at: 1936 5986 1936
Size: 4050 4594
SbfI CC_TGCA'GG
Cuts at: 4285 5996 4285
Size: 1711 6933
ScaI AGT'ACT
Cuts at: 6892 8604 6892
Size: 1712 6932
SfcI C'TryA_G
Cuts at: 826 1045 4281 5125 5200 5861 5992 7127 7808
Size: 219 3236 844 75 661 131 1135 681
Cuts at: 7808 7999 826
Size: 191 1471
Fragments arranged by size:
3236 1471 1135 844 681 661 219 191
131 75
SfoI GGC'GCC
Cuts at: 2350 2389 2350
Size: 39 8605
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8644
SmaI CCC'GGG
Cuts at: 1872 2083 2145 2346 5971 1872
Size: 211 62 201 3625 4545
Fragments arranged by size:
4545 3625 211 201 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3158 3372 4824 6748 7649 7911
Size: 197 926 1215 214 1452 1924 901 262
Cuts at: 7911 820
Size: 1553
Fragments arranged by size:
1924 1553 1452 1215 926 901 262 214
197
SnaBI TAC'GTA
Cuts at: 493 3246 6435 493
Size: 2753 3189 2702
Fragments arranged by size:
3189 2753 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8644
SphI G_CATG'C
Cuts at: 1132 5312 6002 1132
Size: 4180 690 3774
Fragments arranged by size:
4180 3774 690
SrfI GCCC'GGGC
Cuts at: 2346 2346
Size: 8644
SspI AAT'ATT
Cuts at: 5 52 4300 6568 5
Size: 47 4248 2268 2081
Fragments arranged by size:
4248 2268 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8644
StyI C'CwwG_G
Cuts at: 513 1065 2287 2421 3866 4651 6083 513
Size: 552 1222 134 1445 785 1432 3074
Fragments arranged by size:
3074 1445 1432 1222 785 552 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3982 5073 5442 5521 6671 6688
Size: 1255 1067 1335 1091 369 79 1150 17
Cuts at: 6688 6841 7026 7471 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1335 1255 1150 1091 1067 445 369
185 153 79 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2848 2939 3363
Size: 274 80 33 51 1443 871 91 424
Cuts at: 3363 5755 5917 6890 8602 96
Size: 2392 162 973 1712 138
Fragments arranged by size:
2392 1712 1443 973 871 424 274 162
138 91 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8604 8636 8604
Size: 32 8612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8644
XbaI T'CTAG_A
Cuts at: 5980 5980
Size: 8644
XhoI C'TCGA_G
Cuts at: 1943 3372 1943
Size: 1429 7215
XmaI C'CCGG_G
Cuts at: 1870 2081 2143 2344 5969 1870
Size: 211 62 201 3625 4545
Fragments arranged by size:
4545 3625 211 201 62
XmnI GAAnn'nnTTC
Cuts at: 5654 6773 5654
Size: 1119 7525
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6452 276
Size: 53 83 186 936 4918 2468
Fragments arranged by size:
4918 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BsiWI EcoNI EcoRV FseI
NruI PacI PmlI PsrI RsrII SanDI SapI SexAI
SfiI SwaI XcmI