Restriction map for
pFN21AE2328
(Circular) (Six-base) MAPSORT of: pFN21AE2328.seq Check: 4764 from: 1 to: 5770
pFN21AE2328 5770 bp
With 182 enzymes: SgfI *
February 11, 2011 18:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2139 3113 1937
Size: 202 974 4594
Fragments arranged by size:
4594 974 202
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 3833 5217 5485 1632
Size: 1298 903 1384 268 1917
Fragments arranged by size:
1917 1384 1298 903 268
AfeI AGC'GCT
Cuts at: 2075 2075
Size: 5770
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 2586 4669 5646 1203
Size: 1383 2083 977 1327
Fragments arranged by size:
2083 1383 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 2029 4499 2029
Size: 2470 3300
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2582 1184
Size: 1398 4372
AlwNI CAG_nnn'CTG
Cuts at: 2124 2632 2722 3513 5085 2124
Size: 508 90 791 1572 2809
Fragments arranged by size:
2809 1572 791 508 90
ApaLI G'TGCA_C
Cuts at: 2998 3827 4983 2998
Size: 829 1156 3785
Fragments arranged by size:
3785 1156 829
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2852 4324 160
Size: 872 1820 1472 1606
Fragments arranged by size:
1820 1606 1472 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2036 3095 1870
Size: 73 72 21 1059 4545
Fragments arranged by size:
4545 1059 73 72 21
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3089 1391
Size: 1698 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2484 2793 928
Size: 971 161 424 309 3905
Fragments arranged by size:
3905 971 424 309 161
BbvCI CC'TCA_GC
Cuts at: 2114 2114
Size: 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3961 3995 1903
Size: 34 50 34 1940 34 3678
Fragments arranged by size:
3678 1940 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 2723 2780 3187 2723
Size: 57 407 5306
Fragments arranged by size:
5306 407 57
Bme1580I G_kGCm'C
Cuts at: 1790 2987 3002 3831 4987 1790
Size: 1197 15 829 1156 2573
Fragments arranged by size:
2573 1197 1156 829 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 3501 4459
Size: 416 53 83 399 24 639 1445 958
Cuts at: 4459 442
Size: 1753
Fragments arranged by size:
1753 1445 958 639 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 4430 1239
Size: 1259 1932 2579
Fragments arranged by size:
2579 1932 1259
Bpu10I CC'TnA_GC
Cuts at: 2114 2114
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 2776 3561 493
Size: 711 1164 408 785 2702
Fragments arranged by size:
2702 1164 785 711 408
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 1178
Size: 30 165 30 1407 30 4108
Fragments arranged by size:
4108 1407 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5770
BseYI C'CCAG_C
Cuts at: 4973 5469 4973
Size: 496 5274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2756 1163
Size: 573 1020 4177
Fragments arranged by size:
4177 1020 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 3220 3981 5009 1540
Size: 275 186 60 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 2781 3002 3831 3916 4987 2781
Size: 221 829 85 1071 3564
Fragments arranged by size:
3564 1071 829 221 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
BsmBI CGTCTCn'nnnn_
Cuts at: 2600 2600
Size: 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2781 2987 3002 3089 3831 3916 4987
Size: 399 991 206 15 87 742 85 1071
Cuts at: 4987 1391
Size: 2174
Fragments arranged by size:
2174 1071 991 742 399 206 87 85
15
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 2439 3659 5389 2439
Size: 1220 1730 2820
Fragments arranged by size:
2820 1730 1220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2694 2778 3125 844
Size: 1850 84 347 3489
Fragments arranged by size:
3489 1850 347 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2094 2833 3657 1394
Size: 429 271 739 824 3507
Fragments arranged by size:
3507 824 739 429 271
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2790 4414 4601 1700
Size: 110 980 1624 187 2869
Fragments arranged by size:
2869 1624 980 187 110
BsrGI T'GTAC_A
Cuts at: 96 2537 96
Size: 2441 3329
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2124 794
Size: 1330 4440
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2253 2838 3100 3467 3852 3869 5321 5407
Size: 281 585 262 367 385 17 1452 86
Cuts at: 5407 5419 5765 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 346 281 262
86 17 12
BstZ17I GTA'TAC
Cuts at: 2140 2140
Size: 5770
BtgI C'CryG_G
Cuts at: 513 1065 2287 513
Size: 552 1222 3996
Fragments arranged by size:
3996 1222 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2407 487
Size: 683 56 1181 3850
Fragments arranged by size:
3850 1181 683 56
BtsI GCAGTG_nn'
Cuts at: 3000 3286 4080 4100 3000
Size: 286 794 20 4670
Fragments arranged by size:
4670 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 3217 4106
Size: 54 1425 50 275 18 228 1159 889
Cuts at: 4106 5735 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1159 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 3217 1537
Size: 275 246 1159 4090
Fragments arranged by size:
4090 1159 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2186 3700 1353
Size: 731 102 1514 3423
Fragments arranged by size:
3423 1514 731 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 2930 3833 4430 5217 5485 1239
Size: 393 866 432 903 597 787 268 1524
Fragments arranged by size:
1524 903 866 787 597 432 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2018 2753 2018
Size: 735 5035
EcoO109I rG'GnC_Cy
Cuts at: 2737 2737
Size: 5770
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2077 2287 4917 1678
Size: 399 210 2630 2531
Fragments arranged by size:
2630 2531 399 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 4411 4443 4485
Size: 32 135 32 443 32 2364 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 2364 443 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2310 748
Size: 1562 4208
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 4884 5068 1305
Size: 1056 2523 184 2007
Fragments arranged by size:
2523 2007 1056 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2533 2585 2591 3728 4087 4246 518
Size: 648 1367 52 6 1137 359 159 2042
Fragments arranged by size:
2042 1367 1137 648 359 159 52 6
MspA1I CmG'CkG
Cuts at: 1606 2632 2875 3186 3863 5011 5256 1606
Size: 1026 243 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 1026 677 311 245 243
NcoI C'CATG_G
Cuts at: 513 1065 2287 513
Size: 552 1222 3996
Fragments arranged by size:
3996 1222 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2209 2590 3128 4673 1132
Size: 97 980 381 538 1545 2229
Fragments arranged by size:
2229 1545 980 538 381 97
PasI CC'CwG_GG
Cuts at: 1348 2302 1348
Size: 954 4816
PciI A'CATG_T
Cuts at: 2586 4669 2586
Size: 2083 3687
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2079 2975 2079
Size: 896 4874
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PmlI CAC'GTG
Cuts at: 2776 2776
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2737 2737
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2632 2875 2632
Size: 243 5527
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2084 2084
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2275 4018 5730 2275
Size: 1743 1712 2315
Fragments arranged by size:
2315 1743 1712
SexAI A'CCwGG_T
Cuts at: 2739 2739
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 3118 4253 4934 5125 826
Size: 219 2073 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 2038 3097 1872
Size: 166 1059 4545
Fragments arranged by size:
4545 1059 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 2446 3694 5
Size: 47 2394 1248 2081
Fragments arranged by size:
2394 2081 1248 47
StuI AGG'CCT
Cuts at: 1517 2656 2670 1517
Size: 1139 14 4617
Fragments arranged by size:
4617 1139 14
StyI C'CwwG_G
Cuts at: 513 1065 2287 2918 3209 513
Size: 552 1222 631 291 3074
Fragments arranged by size:
3074 1222 631 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2581 3797 3814 3967 4152 4597
Size: 1255 458 543 1216 17 153 185 445
Cuts at: 4597 325
Size: 1498
Fragments arranged by size:
1498 1255 1216 543 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 2537 4016
Size: 274 80 33 51 1443 296 264 1479
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1479 1443 296 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2977 2989 3009 5730 5762 2957
Size: 20 12 20 2721 32 2965
Fragments arranged by size:
2965 2721 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 1316 2741 1316
Size: 1425 4345
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 2036 3095 1870
Size: 166 1059 4545
Fragments arranged by size:
4545 1059 166
XmnI GAAnn'nnTTC
Cuts at: 2075 2156 3899 2075
Size: 81 1743 3946
Fragments arranged by size:
3946 1743 81
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI XcmI