Restriction map for
pFN21AE2326
(Circular) (Six-base) MAPSORT of: pFN21AE2326.seq Check: 2348 from: 1 to: 6283
pFN21AE2326 6283 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3324 4093 278
Size: 53 83 186 936 1788 769 2468
Fragments arranged by size:
2468 1788 936 769 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2635 3303 3417 4346 5730 5998 1632
Size: 1003 668 114 929 1384 268 1917
Fragments arranged by size:
1917 1384 1003 929 668 268 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 3325 5012 3325
Size: 1687 4596
AleI CACnn'nnGTG
Cuts at: 1166 2861 1166
Size: 1695 4588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 2237 2668 2713 3281 4026 5598 2237
Size: 431 45 568 745 1572 2922
Fragments arranged by size:
2922 1572 745 568 431 45
ApaLI G'TGCA_C
Cuts at: 4340 5496 4340
Size: 1156 5127
ApoI r'AATT_y
Cuts at: 1493 3592 3818 3970 6079 1493
Size: 2099 226 152 2109 1697
Fragments arranged by size:
2109 2099 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 2778 3213 3243 3608 1870
Size: 73 835 435 30 365 4545
Fragments arranged by size:
4545 835 435 365 73 30
BamHI G'GATC_C
Cuts at: 2029 2164 3369 3613 3980 2029
Size: 135 1205 244 367 4332
Fragments arranged by size:
4332 1205 367 244 135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2719 3604 5059 618
Size: 325 217 627 272 660 885 1455 1842
Fragments arranged by size:
1842 1455 885 660 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2504 2703 2988 3412 3462 3602 1391
Size: 1113 199 285 424 50 140 4072
Fragments arranged by size:
4072 1113 424 285 199 140 50
BbeI G_GCGC'C
Cuts at: 2063 2063
Size: 6283
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 2724 2879 928
Size: 971 396 429 155 4332
Fragments arranged by size:
4332 971 429 396 155
BbvCI CC'TCA_GC
Cuts at: 2294 2294
Size: 6283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2383 2417 4474 4508 1903
Size: 34 446 34 2057 34 3678
Fragments arranged by size:
3678 2057 446 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3347 3416 4175 5385 3347
Size: 69 759 1210 4245
Fragments arranged by size:
4245 1210 759 69
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2912 4894 136
Size: 107 122 71 631 605 1240 1982 1525
Fragments arranged by size:
1982 1525 1240 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6278 6278
Size: 6283
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 2724 4344 5500 1790
Size: 934 1620 1156 2573
Fragments arranged by size:
2573 1620 1156 934
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4014 4972 442
Size: 416 53 83 399 24 2597 958 1753
Fragments arranged by size:
2597 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2441 2816 3434 4943 1239
Size: 839 363 375 618 1509 2579
Fragments arranged by size:
2579 1509 839 618 375 363
Bpu10I CC'TnA_GC
Cuts at: 2294 2294
Size: 6283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2774 4408 5273 5571 2002
Size: 772 1634 865 298 2714
Fragments arranged by size:
2714 1634 865 772 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 3138 3175 4946 882
Size: 617 1020 619 37 1771 2219
Fragments arranged by size:
2219 1771 1020 619 617 37
BsaAI yAC'GTr
Cuts at: 493 1204 2158 4074 493
Size: 711 954 1916 2702
Fragments arranged by size:
2702 1916 954 711
BsaBI GATnn'nnATC
Cuts at: 3350 6277 3350
Size: 2927 3356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 3321 4090 4472
Size: 53 83 186 936 527 1261 769 382
Cuts at: 4472 5107 275
Size: 635 1451
Fragments arranged by size:
1451 1261 936 769 635 527 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2604 3426 3496 4716 5114 5388 5535 5946 2604
Size: 822 70 1220 398 274 147 411 2941
Fragments arranged by size:
2941 1220 822 411 398 274 147 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2571 2601 3284 3314 1178
Size: 30 165 30 1168 30 683 30 4147
Fragments arranged by size:
4147 1168 683 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2444 3470 3515 1186
Size: 880 378 1026 45 3954
Fragments arranged by size:
3954 1026 880 378 45
BseYI C'CCAG_C
Cuts at: 2497 2813 5486 5982 2497
Size: 316 2673 496 2798
Fragments arranged by size:
2798 2673 496 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5710
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 3509 3733 4494 5522 1540
Size: 275 186 1366 142 224 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 224 186 142
BsiHKAI G_wGCw'C
Cuts at: 3412 3462 4344 4429 5500 3412
Size: 50 882 85 1071 4195
Fragments arranged by size:
4195 1071 882 85 50
BsmI GAATG_Cn'
Cuts at: 3794 3887 3794
Size: 93 6190
BsmBI CGTCTCn'nnnn_
Cuts at: 2040 2040
Size: 6283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2504 2703 2724 2988 3412 3462 3602
Size: 399 714 199 21 264 424 50 140
Cuts at: 3602 4344 4429 5500 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 424 399 264 199
140 85 50 21
BspEI T'CCGG_A
Cuts at: 2604 3496 5946 2604
Size: 892 2450 2941
Fragments arranged by size:
2941 2450 892
BspHI T'CATG_A
Cuts at: 2508 3471 4172 5902 2508
Size: 963 701 1730 2889
Fragments arranged by size:
2889 1730 963 701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2056 3505 4170 1394
Size: 429 233 1449 665 3507
Fragments arranged by size:
3507 1449 665 429 233
BsrDI GCAATG_nn'
Cuts at: 65 1248 3065 4778 4952 65
Size: 1183 1817 1713 174 1396
Fragments arranged by size:
1817 1713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3067 794
Size: 2273 4010
BstBI TT'CG_AA
Cuts at: 3516 5087 3516
Size: 1571 4712
BstEII G'GTnAC_C
Cuts at: 1184 2974 1184
Size: 1790 4493
BstXI CCAn_nnnn'nTGG
Cuts at: 3166 3166
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2029 2164 2601 2772 3369 3613 3980 4365
Size: 57 135 437 171 597 244 367 385
Cuts at: 4365 4382 5834 5920 5932 6278 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 597 437 385 367 346 244
171 135 86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 2874 3799 4593 4613 2874
Size: 925 794 20 4544
Fragments arranged by size:
4544 925 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2954 5290 809
Size: 507 1638 2336 1802
Fragments arranged by size:
2336 1802 1638 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2838 3012
Size: 54 1425 50 275 18 534 474 174
Cuts at: 3012 3364 3506 3552 3730 4619 6248 8
Size: 352 142 46 178 889 1629 43
Fragments arranged by size:
1629 1425 889 534 474 352 275 178
174 142 54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 3506 3730 1537
Size: 275 1552 142 224 4090
Fragments arranged by size:
4090 1552 275 224 142
EarI CTCTTCn'nnn_
Cuts at: 1353 4213 1353
Size: 2860 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3410 3460 3410
Size: 50 6233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2441 2635 2816 3303 3417 3434
Size: 393 446 363 194 181 487 114 17
Cuts at: 3434 4346 4943 5730 5998 1239
Size: 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 487 446 393 363
268 194 181 114 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2915 3378 2915
Size: 463 5820
EcoO109I rG'GnC_Cy
Cuts at: 2189 2189
Size: 6283
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2562 2562
Size: 6283
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 2017 2063 2136 2473 5430 1678
Size: 339 46 73 337 2957 2531
Fragments arranged by size:
2957 2531 339 337 73 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2920 2952 3311
Size: 32 135 32 584 32 732 32 359
Cuts at: 3311 3343 3394 3426 4924 4956 4998 5030 1373
Size: 32 51 32 1498 32 42 32 2626
Fragments arranged by size:
2626 1498 732 584 359 135 51 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2413 3627 3873 669
Size: 988 281 475 1214 246 3079
Fragments arranged by size:
3079 1214 988 475 281 246
HindIII A'AGCT_T
Cuts at: 748 3022 748
Size: 2274 4009
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KasI G'GCGC_C
Cuts at: 2059 2059
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2080 5397 5581 1305
Size: 775 3317 184 2007
Fragments arranged by size:
3317 2007 775 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2840 3014 10
Size: 54 1425 343 534 474 174 3279
Fragments arranged by size:
3279 1425 534 474 343 174 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 2861 2897 3473 4241 4600 4759
Size: 648 1083 612 36 576 768 359 159
Cuts at: 4759 518
Size: 2042
Fragments arranged by size:
2042 1083 768 648 612 576 359 159
36
MspA1I CmG'CkG
Cuts at: 1606 3699 4376 5524 5769 1606
Size: 2093 677 1148 245 2120
Fragments arranged by size:
2120 2093 1148 677 245
NarI GG'CG_CC
Cuts at: 2060 2060
Size: 6283
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4792
Fragments arranged by size:
4792 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2497 2731 2896 3641 5186 1132
Size: 97 1268 234 165 745 1545 2229
Fragments arranged by size:
2229 1545 1268 745 234 165 97
PasI CC'CwG_GG
Cuts at: 1348 3380 1348
Size: 2032 4251
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 2479 3270 1108
Size: 1212 159 791 4121
Fragments arranged by size:
4121 1212 791 159
PfoI T'CCnGG_A
Cuts at: 2514 2514
Size: 6283
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PstI C_TGCA'G
Cuts at: 830 2664 2943 3635 830
Size: 1834 279 692 3478
Fragments arranged by size:
3478 1834 692 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
SacI G_AGCT'C
Cuts at: 3412 3462 3412
Size: 50 6233
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 2660 2939 3631 4766 5447 5638 826
Size: 219 1615 279 692 1135 681 191 1471
Fragments arranged by size:
1615 1471 1135 692 681 279 219 191
SfoI GGC'GCC
Cuts at: 2061 2061
Size: 6283
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3215 3610 1872
Size: 1343 395 4545
Fragments arranged by size:
4545 1343 395
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2017 2789 4387 5288 5550 820
Size: 197 926 74 772 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 772 262 197 74
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3641 1132
Size: 2509 3774
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 4894
StyI C'CwwG_G
Cuts at: 513 1065 2004 2750 2859 2979 3722 513
Size: 552 939 746 109 120 743 3074
Fragments arranged by size:
3074 939 746 743 552 120 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4310 4327 4480 4665 5110 325
Size: 1255 2730 17 153 185 445 1498
Fragments arranged by size:
2730 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3234 4529 6241
Size: 274 80 33 51 1443 1257 1295 1712
Cuts at: 6241 96
Size: 138
Fragments arranged by size:
1712 1443 1295 1257 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 6243 6275 2571
Size: 32 3640 32 2579
Fragments arranged by size:
3640 2579 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 3213 3608 1870
Size: 1343 395 4545
Fragments arranged by size:
4545 1343 395
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3322 4091 276
Size: 53 83 186 936 1788 769 2468
Fragments arranged by size:
2468 1788 936 769 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI