Restriction map for
pFN21AE2323
(Circular) (Six-base) MAPSORT of: pFN21AE2323.seq Check: 6281 from: 1 to: 9829
pFN21AE2323 9829 bp
With 182 enzymes: SgfI *
January 27, 2011 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7639 278
Size: 53 83 186 936 6103 2468
Fragments arranged by size:
6103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2983 4632 6832 7172 1937
Size: 1046 1649 2200 340 4594
Fragments arranged by size:
4594 2200 1649 1046 340
Acc65I G'GTAC_C
Cuts at: 7150 7150
Size: 9829
AclI AA'CG_TT
Cuts at: 1563 7956 8329 1563
Size: 6393 373 3063
Fragments arranged by size:
6393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2576 2832 3870 4329 6069 7892 9276
Size: 422 522 256 1038 459 1740 1823 1384
Cuts at: 9276 9544 1632
Size: 268 1917
Fragments arranged by size:
1917 1823 1740 1384 1038 522 459 422
268 256
AfeI AGC'GCT
Cuts at: 2322 2322
Size: 9829
AflII C'TTAA_G
Cuts at: 820 1017 2116 3566 3668 6154 820
Size: 197 1099 1450 102 2486 4495
Fragments arranged by size:
4495 2486 1450 1099 197 102
AflIII A'CryG_T
Cuts at: 1203 8728 9705 1203
Size: 7525 977 1327
Fragments arranged by size:
7525 1327 977
AgeI A'CCGG_T
Cuts at: 5154 8660 5154
Size: 3506 6323
AhdI GACnn_n'nnGTC
Cuts at: 8558 8558
Size: 9829
AleI CACnn'nnGTG
Cuts at: 1166 6823 1166
Size: 5657 4172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9829
AlwNI CAG_nnn'CTG
Cuts at: 4178 7076 7572 9144 4178
Size: 2898 496 1572 4863
Fragments arranged by size:
4863 2898 1572 496
ApaLI G'TGCA_C
Cuts at: 7886 9042 7886
Size: 1156 8673
ApoI r'AATT_y
Cuts at: 1493 2760 2920 3833 4161 4238 5251 5302 5555
Size: 1267 160 913 328 77 1013 51 253
Cuts at: 5555 5566 5851 6436 6484 6585 7138 7364 7516
Size: 11 285 585 48 101 553 226 152
Cuts at: 7516 9625 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1267 1013 913 585 553 328
285 253 226 160 152 101 77 51
48 11
AseI AT'TA_AT
Cuts at: 160 1032 2510 2901 6198 6867 8383 160
Size: 872 1478 391 3297 669 1516 1606
Fragments arranged by size:
3297 1606 1516 1478 872 669 391
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9829
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 7154 1870
Size: 73 154 5057 4545
Fragments arranged by size:
5057 4545 154 73
BamHI G'GATC_C
Cuts at: 2928 7159 7526 2928
Size: 4231 367 5231
Fragments arranged by size:
5231 4231 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5866 7150 8605 618
Size: 325 217 627 4079 1284 1455 1842
Fragments arranged by size:
4079 1842 1455 1284 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4926 5415 5636 5705 7148 1391
Size: 3535 489 221 69 1443 4072
Fragments arranged by size:
4072 3535 1443 489 221 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2835 3005 3470 3736 3932 4347 5520
Size: 971 936 170 465 266 196 415 1173
Cuts at: 5520 6398 6534 928
Size: 878 136 4223
Fragments arranged by size:
4223 1173 971 936 878 465 415 266
196 170 136
BbvCI CC'TCA_GC
Cuts at: 3117 3117
Size: 9829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8020 8054 1903
Size: 34 6083 34 3678
Fragments arranged by size:
6083 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3565 6942 7721 8931 3565
Size: 3377 779 1210 4463
Fragments arranged by size:
4463 3377 1210 779
BclI T'GATC_A
Cuts at: 1548 3253 1548
Size: 1705 8124
BfrBI ATG'CAT
Cuts at: 2565 6028 6164 7060 2565
Size: 3463 136 896 5334
Fragments arranged by size:
5334 3463 896 136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 8440 136
Size: 107 122 71 631 605 337 6431 1525
Fragments arranged by size:
6431 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3877 9824 3877
Size: 5947 3882
BlpI GC'TnA_GC
Cuts at: 6757 7246 6757
Size: 489 9340
Bme1580I G_kGCm'C
Cuts at: 1790 7890 9046 1790
Size: 6100 1156 2573
Fragments arranged by size:
6100 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4630 1318
Size: 3312 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2243 4981 5409
Size: 416 53 83 399 24 826 2738 428
Cuts at: 5409 6915 7560 8518 442
Size: 1506 645 958 1753
Fragments arranged by size:
2738 1753 1506 958 826 645 428 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6765 1056
Size: 5709 4120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2144 2144
Size: 9829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2339 5856 6048 6441 8489 1239
Size: 1090 10 3517 192 393 2048 2579
Fragments arranged by size:
3517 2579 2048 1090 393 192 10
Bpu10I CC'TnA_GC
Cuts at: 3117 3465 3117
Size: 348 9481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5689 6732 7954 8819 9117 5689
Size: 1043 1222 865 298 6401
Fragments arranged by size:
6401 1222 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 8492 882
Size: 617 3327 3666 2219
Fragments arranged by size:
3666 3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5651 7620 493
Size: 711 4447 1969 2702
Fragments arranged by size:
4447 2702 1969 711
BsaBI GATnn'nnATC
Cuts at: 3534 5427 9823 3534
Size: 1893 4396 3540
Fragments arranged by size:
4396 3540 1893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5816 7636 8018 8653
Size: 53 83 186 936 4283 1820 382 635
Cuts at: 8653 275
Size: 1451
Fragments arranged by size:
4283 1820 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4566 5154 8262 8660 8934 9081 9492 4566
Size: 588 3108 398 274 147 411 4903
Fragments arranged by size:
4903 3108 588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6414 6444 1178
Size: 30 165 30 5011 30 4563
Fragments arranged by size:
5011 4563 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 3050 1186
Size: 901 963 7965
Fragments arranged by size:
7965 963 901
BseYI C'CCAG_C
Cuts at: 3752 4275 6602 9032 9528 3752
Size: 523 2327 2430 496 4053
Fragments arranged by size:
4053 2430 2327 523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3435 6976 1163
Size: 573 1699 3541 4016
Fragments arranged by size:
4016 3541 1699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2020 2026 7279 8040 9068
Size: 275 186 15 4 6 5253 761 1028
Cuts at: 9068 1540
Size: 2301
Fragments arranged by size:
5253 2301 1028 761 275 186 15 6
4
BsiHKAI G_wGCw'C
Cuts at: 5636 5705 5905 7890 7975 9046 5636
Size: 69 200 1985 85 1071 6419
Fragments arranged by size:
6419 1985 1071 200 85 69
BsmI GAATG_Cn'
Cuts at: 2479 6030 7340 7433 2479
Size: 3551 1310 93 4875
Fragments arranged by size:
4875 3551 1310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4926 5415 5636 5705 5905 7148 7890
Size: 399 3136 489 221 69 200 1243 742
Cuts at: 7890 7975 9046 1391
Size: 85 1071 2174
Fragments arranged by size:
3136 2174 1243 1071 742 489 399 221
200 85 69
BspEI T'CCGG_A
Cuts at: 9492 9492
Size: 9829
BspHI T'CATG_A
Cuts at: 6144 6898 7718 9448 6144
Size: 754 820 1730 6525
Fragments arranged by size:
6525 1730 820 754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 4644 6129 7184 844
Size: 2038 1762 1485 1055 3489
Fragments arranged by size:
3489 2038 1762 1485 1055
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 7716 1394
Size: 429 205 5688 3507
Fragments arranged by size:
5688 3507 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 8324 8498 65
Size: 1183 1317 5759 174 1396
Fragments arranged by size:
5759 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5154 8473 8660 1700
Size: 110 3344 3319 187 2869
Fragments arranged by size:
3344 3319 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5461 7042 96
Size: 5365 1581 2883
Fragments arranged by size:
5365 2883 1581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9829
BssSI C'ACGA_G
Cuts at: 5715 7889 8901 5715
Size: 2174 1012 6643
Fragments arranged by size:
6643 2174 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2567 794
Size: 1773 8056
BstBI TT'CG_AA
Cuts at: 8633 8633
Size: 9829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9829
BstXI CCAn_nnnn'nTGG
Cuts at: 2860 4016 2860
Size: 1156 8673
BstYI r'GATC_y
Cuts at: 1972 2089 2928 3064 3149 3778 3877 7159 7526
Size: 117 839 136 85 629 99 3282 367
Cuts at: 7526 7911 7928 9380 9466 9478 9824 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3282 1977 1452 839 629 385 367 346
136 117 99 86 85 17 12
BstZ17I GTA'TAC
Cuts at: 6833 6833
Size: 9829
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 6237 513
Size: 552 939 16 4217 4105
Fragments arranged by size:
4217 4105 939 552 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9090
Fragments arranged by size:
9090 683 56
BtsI GCAGTG_nn'
Cuts at: 3343 4048 5347 6739 6988 7345 8139 8159 3343
Size: 705 1299 1392 249 357 794 20 5013
Fragments arranged by size:
5013 1392 1299 794 705 357 249 20
ClaI AT'CG_AT
Cuts at: 1554 2652 1554
Size: 1098 8731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9794
DraI TTT'AAA
Cuts at: 3432 7133 3432
Size: 3701 6128
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 5821 7008 8836 809
Size: 507 1236 3269 1187 1828 1802
Fragments arranged by size:
3269 1828 1802 1236 1187 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2023 2139
Size: 54 1425 50 275 18 187 6 116
Cuts at: 2139 2215 3674 5006 7276 8165 9794 8
Size: 76 1459 1332 2270 889 1629 43
Fragments arranged by size:
2270 1629 1459 1425 1332 889 275 187
116 76 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2017 2023 7276 1537
Size: 275 205 6 5253 4090
Fragments arranged by size:
5253 4090 275 205 6
EarI CTCTTCn'nnn_
Cuts at: 1353 3846 5893 7759 1353
Size: 2493 2047 1866 3423
Fragments arranged by size:
3423 2493 2047 1866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 8416 8790 8936 387
Size: 1064 138 482 6345 374 146 1280
Fragments arranged by size:
6345 1280 1064 482 374 146 138
EcoICRI GAG'CTC
Cuts at: 5634 5703 5634
Size: 69 9760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2329 2339 2576 2832 3870 4329
Size: 393 422 275 10 237 256 1038 459
Cuts at: 4329 5856 6048 6069 6441 7892 8489 9276 9544
Size: 1527 192 21 372 1451 597 787 268
Cuts at: 9544 1239
Size: 1524
Fragments arranged by size:
1527 1524 1451 1038 787 597 459 422
393 372 275 268 256 237 192 21
10
EcoO109I rG'GnC_Cy
Cuts at: 2680 3461 4902 2680
Size: 781 1441 7607
Fragments arranged by size:
7607 1441 781
EcoRI G'AATT_C
Cuts at: 4238 6484 7138 7516 4238
Size: 2246 654 378 6551
Fragments arranged by size:
6551 2246 654 378
EcoRV GAT'ATC
Cuts at: 2254 2330 3385 4321 5194 2254
Size: 76 1055 936 873 6889
Fragments arranged by size:
6889 1055 936 873 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3650 4799 3650
Size: 1149 8680
FspI TGC'GCA
Cuts at: 8335 8335
Size: 9829
HaeII r_GCGC'y
Cuts at: 1678 2324 8976 1678
Size: 646 6652 2531
Fragments arranged by size:
6652 2531 646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 2920 2952 3245
Size: 32 135 32 747 32 569 32 293
Cuts at: 3245 3261 3277 3293 4975 5007 5692 5724 6728
Size: 16 16 16 1682 32 685 32 1004
Cuts at: 6728 6760 8470 8502 8544 8576 1373
Size: 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 1682 1004 747 685 569 293
135 42 32 32 32 32 32 32
32 32 32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3974 4525 5088 7173 7419 669
Size: 988 281 2036 551 563 2085 246 3079
Fragments arranged by size:
3079 2085 2036 988 563 551 281 246
HindIII A'AGCT_T
Cuts at: 748 2198 6051 748
Size: 1450 3853 4526
Fragments arranged by size:
4526 3853 1450
HpaI GTT'AAC
Cuts at: 7419 7419
Size: 9829
KpnI G_GTAC'C
Cuts at: 7154 7154
Size: 9829
MfeI C'AATT_G
Cuts at: 3507 4484 7428 3507
Size: 977 2944 5908
Fragments arranged by size:
5908 2944 977
MluI A'CGCG_T
Cuts at: 9705 9705
Size: 9829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2500 3068 4580 4671 6992 8943 9127 1305
Size: 1195 568 1512 91 2321 1951 184 2007
Fragments arranged by size:
2321 2007 1951 1512 1195 568 184 91
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 2217 3676 5008 10
Size: 54 1425 343 309 76 1459 1332 4831
Fragments arranged by size:
4831 1459 1425 1332 343 309 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4014 4370 4650 5106 5436 6098 6498
Size: 648 2848 356 280 456 330 662 400
Cuts at: 6498 6552 6823 7787 8146 8305 518
Size: 54 271 964 359 159 2042
Fragments arranged by size:
2848 2042 964 662 648 456 400 359
356 330 280 271 159 54
MspA1I CmG'CkG
Cuts at: 1606 2022 2067 2086 2810 3116 7245 7922 9070
Size: 416 45 19 724 306 4129 677 1148
Cuts at: 9070 9315 1606
Size: 245 2120
Fragments arranged by size:
4129 2120 1148 724 677 416 306 245
45 19
NcoI C'CATG_G
Cuts at: 513 1065 2004 6237 513
Size: 552 939 4233 4105
Fragments arranged by size:
4233 4105 939 552
NdeI CA'TA_TG
Cuts at: 387 3300 5640 6256 6953 387
Size: 2913 2340 616 697 3263
Fragments arranged by size:
3263 2913 2340 697 616
NheI G'CTAG_C
Cuts at: 1052 6761 1052
Size: 5709 4120
NotI GC'GGCC_GC
Cuts at: 2023 7276 2023
Size: 5253 4576
NsiI A_TGCA'T
Cuts at: 2567 6030 6166 7062 2567
Size: 3463 136 896 5334
Fragments arranged by size:
5334 3463 896 136
NspI r_CATG'y
Cuts at: 1132 1229 2565 4338 7187 8732 1132
Size: 97 1336 1773 2849 1545 2229
Fragments arranged by size:
2849 2229 1773 1545 1336 97
PacI TTA_AT'TAA
Cuts at: 3594 3594
Size: 9829
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9829
PciI A'CATG_T
Cuts at: 8728 8728
Size: 9829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 4690 5537 1108
Size: 1029 2553 847 5400
Fragments arranged by size:
5400 2553 1029 847
PmeI GTTT'AAAC
Cuts at: 7133 7133
Size: 9829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7898 7930 9411 9443 7898
Size: 32 1481 32 8284
Fragments arranged by size:
8284 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3461 4902 3461
Size: 1441 8388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9829
PsiI TTA'TAA
Cuts at: 7399 7399
Size: 9829
PstI C_TGCA'G
Cuts at: 830 4174 4190 7181 830
Size: 3344 16 2991 3478
Fragments arranged by size:
3478 3344 2991 16
PvuI CG_AT'CG
Cuts at: 2001 2016 2001
Size: 15 9814
PvuII CAG'CTG
Cuts at: 2810 3116 2810
Size: 306 9523
SacI G_AGCT'C
Cuts at: 5636 5705 5636
Size: 69 9760
SacII CC_GC'GG
Cuts at: 2023 2023
Size: 9829
SalI G'TCGA_C
Cuts at: 1936 7171 1936
Size: 5235 4594
SapI GCTCTTCn'nnn_
Cuts at: 5893 5893
Size: 9829
SbfI CC_TGCA'GG
Cuts at: 7181 7181
Size: 9829
ScaI AGT'ACT
Cuts at: 2949 5773 5785 8077 9789 2949
Size: 2824 12 2292 1712 2989
Fragments arranged by size:
2989 2824 2292 1712 12
SfcI C'TryA_G
Cuts at: 826 1045 2788 4123 4170 4186 4832 6011 6539
Size: 219 1743 1335 47 16 646 1179 528
Cuts at: 6539 7104 7177 8312 8993 9184 826
Size: 565 73 1135 681 191 1471
Fragments arranged by size:
1743 1471 1335 1179 1135 681 646 565
528 219 191 73 47 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9829
SmaI CCC'GGG
Cuts at: 1872 7156 1872
Size: 5284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 3566 3668 5704 6154 6747
Size: 197 926 173 1450 102 2036 450 593
Cuts at: 6747 7933 8834 9096 820
Size: 1186 901 262 1553
Fragments arranged by size:
2036 1553 1450 1186 926 901 593 450
262 197 173 102
SnaBI TAC'GTA
Cuts at: 493 5651 7620 493
Size: 5158 1969 2702
Fragments arranged by size:
5158 2702 1969
SpeI A'CTAG_T
Cuts at: 152 4660 5174 152
Size: 4508 514 4807
Fragments arranged by size:
4807 4508 514
SphI G_CATG'C
Cuts at: 1132 2565 7187 1132
Size: 1433 4622 3774
Fragments arranged by size:
4622 3774 1433
SspI AAT'ATT
Cuts at: 5 52 4705 7753 5
Size: 47 4653 3048 2081
Fragments arranged by size:
4653 3048 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9829
StyI C'CwwG_G
Cuts at: 513 1065 2004 3390 3792 4393 4791 6237 7268
Size: 552 939 1386 402 601 398 1446 1031
Cuts at: 7268 513
Size: 3074
Fragments arranged by size:
3074 1446 1386 1031 939 601 552 402
398
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5241 5464 7856 7873 8026 8211 8656
Size: 1255 3661 223 2392 17 153 185 445
Cuts at: 8656 325
Size: 1498
Fragments arranged by size:
3661 2392 1498 1255 445 223 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2947 4663 4696
Size: 274 80 33 51 1443 970 1716 33
Cuts at: 4696 4915 5177 5461 5525 5771 5783 6521 7042
Size: 219 262 284 64 246 12 738 521
Cuts at: 7042 8075 9787 96
Size: 1033 1712 138
Fragments arranged by size:
1716 1712 1443 1033 970 738 521 284
274 262 246 219 138 80 64 51
33 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3301 3333 9789 9821 3301
Size: 32 6456 32 3309
Fragments arranged by size:
6456 3309 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2796 1316
Size: 1480 8349
XbaI T'CTAG_A
Cuts at: 5096 5975 7165 5096
Size: 879 1190 7760
Fragments arranged by size:
7760 1190 879
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3778 6660 3778
Size: 2882 6947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9829
XmaI C'CCGG_G
Cuts at: 1870 7154 1870
Size: 5284 4545
XmnI GAAnn'nnTTC
Cuts at: 3837 4000 6699 7032 7958 3837
Size: 163 2699 333 926 5708
Fragments arranged by size:
5708 2699 926 333 163
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7637 276
Size: 53 83 186 936 6103 2468
Fragments arranged by size:
6103 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PfoI PmlI PspOMI PspXI PsrI
RsrII SanDI SexAI SfiI SfoI SrfI SwaI