Restriction map for
pFN21AE2322
(Circular) (Six-base) MAPSORT of: pFN21AE2322.seq Check: 422 from: 1 to: 8710
pFN21AE2322 8710 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3307 3307
Size: 8710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6520 278
Size: 53 83 186 936 4984 2468
Fragments arranged by size:
4984 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 5580 6053 1937
Size: 646 2997 473 4594
Fragments arranged by size:
4594 2997 646 473
Acc65I G'GTAC_C
Cuts at: 6031 6031
Size: 8710
AclI AA'CG_TT
Cuts at: 1563 6837 7210 1563
Size: 5274 373 3063
Fragments arranged by size:
5274 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2998 3455 3650 5738 6773 8157 8425 1632
Size: 1366 457 195 2088 1035 1384 268 1917
Fragments arranged by size:
2088 1917 1384 1366 1035 457 268 195
AfeI AGC'GCT
Cuts at: 3802 3802
Size: 8710
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8513
AflIII A'CryG_T
Cuts at: 1203 7609 8586 1203
Size: 6406 977 1327
Fragments arranged by size:
6406 1327 977
AgeI A'CCGG_T
Cuts at: 7541 7541
Size: 8710
AhdI GACnn_n'nnGTC
Cuts at: 7439 7439
Size: 8710
AleI CACnn'nnGTG
Cuts at: 1166 3881 1166
Size: 2715 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4476 1184
Size: 3292 5418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4961 4993 4961
Size: 32 8678
AlwNI CAG_nnn'CTG
Cuts at: 2653 3322 3756 4228 5142 5289 6453 8025 2653
Size: 669 434 472 914 147 1164 1572 3338
Fragments arranged by size:
3338 1572 1164 914 669 472 434 147
ApaI G_GGCC'C
Cuts at: 2356 4805 2356
Size: 2449 6261
ApaLI G'TGCA_C
Cuts at: 2247 3335 4141 4610 6767 7923 2247
Size: 1088 806 469 2157 1156 3034
Fragments arranged by size:
3034 2157 1156 1088 806 469
ApoI r'AATT_y
Cuts at: 1493 2317 3049 5093 5296 6019 6245 6397 8506
Size: 824 732 2044 203 723 226 152 2109
Cuts at: 8506 1493
Size: 1697
Fragments arranged by size:
2109 2044 1697 824 732 723 226 203
152
AseI AT'TA_AT
Cuts at: 160 1032 7264 160
Size: 872 6232 1606
Fragments arranged by size:
6232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8710
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3456 3842 4319 6035 1870
Size: 73 1048 465 386 477 1716 4545
Fragments arranged by size:
4545 1716 1048 477 465 386 73
BamHI G'GATC_C
Cuts at: 6040 6407 6040
Size: 367 8343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2467 2863 4724 5276 5909
Size: 325 217 627 680 396 1861 552 633
Cuts at: 5909 6031 7486 618
Size: 122 1455 1842
Fragments arranged by size:
1861 1842 1455 680 633 627 552 396
325 217 122
BanII G_rGCy'C
Cuts at: 1391 2356 3452 3974 4169 4634 4805 5349 5426
Size: 965 1096 522 195 465 171 544 77
Cuts at: 5426 5862 6029 1391
Size: 436 167 4072
Fragments arranged by size:
4072 1096 965 544 522 465 436 195
171 167 77
BbeI G_GCGC'C
Cuts at: 5913 5913
Size: 8710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2177 2240 2494 3856 928
Size: 971 278 63 254 1362 5782
Fragments arranged by size:
5782 1362 971 278 254 63
BbvCI CC'TCA_GC
Cuts at: 4280 4280
Size: 8710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6901 6935 1903
Size: 34 4964 34 3678
Fragments arranged by size:
4964 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6602 7812 6602
Size: 1210 7500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2744 4523 4722
Size: 107 122 71 631 605 1072 1779 199
Cuts at: 4722 7321 136
Size: 2599 1525
Fragments arranged by size:
2599 1779 1525 1072 631 605 199 122
107 71
BglII A'GATC_T
Cuts at: 8705 8705
Size: 8710
BlpI GC'TnA_GC
Cuts at: 6127 6127
Size: 8710
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2356 2472 3339 4145 4614 4727 4805
Size: 461 105 116 867 806 469 113 78
Cuts at: 4805 6771 7927 1790
Size: 1966 1156 2573
Fragments arranged by size:
2573 1966 1156 867 806 469 461 116
113 105 78
BmgBI CAC'GTC
Cuts at: 1318 2409 2556 4489 5434 1318
Size: 1091 147 1933 945 4594
Fragments arranged by size:
4594 1933 1091 945 147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4118 5164 5404
Size: 416 53 83 399 24 2701 1046 240
Cuts at: 5404 6441 7399 442
Size: 1037 958 1753
Fragments arranged by size:
2701 1753 1046 1037 958 416 399 240
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 2675 3428 3713 4077 4645 4909 4964
Size: 931 505 753 285 364 568 264 55
Cuts at: 4964 7370 1239
Size: 2406 2579
Fragments arranged by size:
2579 2406 931 753 568 505 364 285
264 55
Bpu10I CC'TnA_GC
Cuts at: 2137 4280 5425 2137
Size: 2143 1145 5422
Fragments arranged by size:
5422 2143 1145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6835 7700 7998 6835
Size: 865 298 7547
Fragments arranged by size:
7547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 4486 5399 7373 882
Size: 617 2844 143 913 1974 2219
Fragments arranged by size:
2844 2219 1974 913 617 143
BsaAI yAC'GTr
Cuts at: 493 1204 3123 3430 6501 493
Size: 711 1919 307 3071 2702
Fragments arranged by size:
3071 2702 1919 711 307
BsaBI GATnn'nnATC
Cuts at: 8704 8704
Size: 8710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2871 2924 4643 5076
Size: 53 83 186 936 1338 53 1719 433
Cuts at: 5076 5910 6517 6899 7534 275
Size: 834 607 382 635 1451
Fragments arranged by size:
1719 1451 1338 936 834 635 607 433
382 186 83 53 53
BsaWI w'CCGG_w
Cuts at: 3597 3981 5445 7143 7541 7815 7962 8373 3597
Size: 384 1464 1698 398 274 147 411 3934
Fragments arranged by size:
3934 1698 1464 411 398 384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 4550 4580 4955
Size: 30 165 30 1173 30 1944 30 375
Cuts at: 4955 4961 4985 4991 5963 5993 1178
Size: 6 24 6 972 30 3895
Fragments arranged by size:
3895 1944 1173 972 375 165 30 30
30 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 3815 4343 4375 4555 5388 5405 1186
Size: 1261 1368 528 32 180 833 17 4491
Fragments arranged by size:
4491 1368 1261 833 528 180 32 17
BseYI C'CCAG_C
Cuts at: 3951 4739 4908 5010 5378 7913 8409 3951
Size: 788 169 102 368 2535 496 4252
Fragments arranged by size:
4252 2535 788 496 368 169 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 3073 4024 4436 4889 5498 1163
Size: 573 653 684 951 412 453 609 4375
Fragments arranged by size:
4375 951 684 653 609 573 453 412
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4544 4721 6160 6921 7949 1540
Size: 275 186 2543 177 1439 761 1028 2301
Fragments arranged by size:
2543 2301 1439 1028 761 275 186 177
BsiHKAI G_wGCw'C
Cuts at: 2251 2988 3339 3452 4145 4169 4614 4938 5426
Size: 737 351 113 693 24 445 324 488
Cuts at: 5426 5634 5862 6771 6856 7927 2251
Size: 208 228 909 85 1071 3034
Fragments arranged by size:
3034 1071 909 737 693 488 445 351
324 228 208 113 85 24
BsmI GAATG_Cn'
Cuts at: 2264 3248 5612 6221 6314 2264
Size: 984 2364 609 93 4660
Fragments arranged by size:
4660 2364 984 609 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5424 5424
Size: 8710
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2251 2356 2472 2988 3339 3452 3974
Size: 399 461 105 116 516 351 113 522
Cuts at: 3974 4145 4169 4614 4634 4727 4805 4938 5349
Size: 171 24 445 20 93 78 133 411
Cuts at: 5349 5426 5634 5862 6029 6771 6856 7927 1391
Size: 77 208 228 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 516 461 445 411
399 351 228 208 171 167 133 116
113 105 93 85 78 77 24 20
BspEI T'CCGG_A
Cuts at: 3981 5445 8373 3981
Size: 1464 2928 4318
Fragments arranged by size:
4318 2928 1464
BspHI T'CATG_A
Cuts at: 2094 3805 4917 5418 5616 6599 8329 2094
Size: 1711 1112 501 198 983 1730 2475
Fragments arranged by size:
2475 1730 1711 1112 983 501 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 3307 6065 844
Size: 1712 751 2758 3489
Fragments arranged by size:
3489 2758 1712 751
BsrBI CCG'CTC
Cuts at: 1394 1823 4966 5538 6597 1394
Size: 429 3143 572 1059 3507
Fragments arranged by size:
3507 3143 1059 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3678 7205 7379 65
Size: 1183 2430 3527 174 1396
Fragments arranged by size:
3527 2430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 4690 4716 5408 7354 7541 1700
Size: 110 1529 1351 26 692 1946 187 2869
Fragments arranged by size:
2869 1946 1529 1351 692 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8710
BssSI C'ACGA_G
Cuts at: 3514 3530 5206 6770 7782 3514
Size: 16 1676 1564 1012 4442
Fragments arranged by size:
4442 1676 1564 1012 16
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3718 4038 794
Size: 2924 320 5466
Fragments arranged by size:
5466 2924 320
BstBI TT'CG_AA
Cuts at: 3046 7514 3046
Size: 4468 4242
BstEII G'GTnAC_C
Cuts at: 1184 5329 1184
Size: 4145 4565
BstXI CCAn_nnnn'nTGG
Cuts at: 3274 3691 3946 4678 3274
Size: 417 255 732 7306
Fragments arranged by size:
7306 732 417 255
BstYI r'GATC_y
Cuts at: 1972 2895 2946 2995 3495 3753 4923 4953 5448
Size: 923 51 49 500 258 1170 30 495
Cuts at: 5448 6040 6407 6792 6809 8261 8347 8359 8705
Size: 592 367 385 17 1452 86 12 346
Cuts at: 8705 1972
Size: 1977
Fragments arranged by size:
1977 1452 1170 923 592 500 495 385
367 346 258 86 51 49 30 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2461 3267 3975 4044 4863 5220
Size: 552 939 457 806 708 69 819 357
Cuts at: 5220 5955 513
Size: 735 3268
Fragments arranged by size:
3268 939 819 806 735 708 552 457
357 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2956 4498 5101 487
Size: 683 56 1730 1542 603 4096
Fragments arranged by size:
4096 1730 1542 683 603 56
BtsI GCAGTG_nn'
Cuts at: 3204 3399 3510 5214 6226 7020 7040 3204
Size: 195 111 1704 1012 794 20 4874
Fragments arranged by size:
4874 1704 1012 794 195 111 20
ClaI AT'CG_AT
Cuts at: 1554 2268 1554
Size: 714 7996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8675
DraI TTT'AAA
Cuts at: 6014 6014
Size: 8710
DraIII CAC_nnn'GTG
Cuts at: 2467 3934 5112 5686 2467
Size: 1467 1178 574 5491
Fragments arranged by size:
5491 1467 1178 574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5518 7717 809
Size: 507 4202 2199 1802
Fragments arranged by size:
4202 2199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2458 2902 3467
Size: 54 1425 50 275 18 628 444 565
Cuts at: 3467 3544 3735 3936 4491 4541 4680 4714 4718
Size: 77 191 201 555 50 139 34 4
Cuts at: 4718 5236 5262 5677 5952 6157 7046 8675 8
Size: 518 26 415 275 205 889 1629 43
Fragments arranged by size:
1629 1425 889 628 565 555 518 444
415 275 275 205 201 191 139 77
54 50 50 43 34 26 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4541 4718 6157 1537
Size: 275 2729 177 1439 4090
Fragments arranged by size:
4090 2729 1439 275 177
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 3005 3017 4059 4926 6640 1353
Size: 684 968 12 1042 867 1714 3423
Fragments arranged by size:
3423 1714 1042 968 867 684 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3077 3539 3587 4849 5813 7297
Size: 1064 138 1488 462 48 1262 964 1484
Cuts at: 7297 7671 7817 387
Size: 374 146 1280
Fragments arranged by size:
1488 1484 1280 1262 1064 964 462 374
146 138 48
EcoICRI GAG'CTC
Cuts at: 3450 4167 5424 5860 3450
Size: 717 1257 436 6300
Fragments arranged by size:
6300 1257 717 436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2170 2675 2998 3428 3455 3650 3713
Size: 393 538 505 323 430 27 195 63
Cuts at: 3713 4077 4645 4909 4964 5738 6773 7370 8157
Size: 364 568 264 55 774 1035 597 787
Cuts at: 8157 8425 1239
Size: 268 1524
Fragments arranged by size:
1524 1035 787 774 597 568 538 505
430 393 364 323 268 264 195 63
55 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 3533 2045
Size: 1488 7222
EcoO109I rG'GnC_Cy
Cuts at: 2285 2352 3151 3383 4733 4801 2285
Size: 67 799 232 1350 68 6194
Fragments arranged by size:
6194 1350 799 232 68 67
EcoRI G'AATT_C
Cuts at: 6019 6397 6019
Size: 378 8332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3680 3680
Size: 8710
FseI GG_CCGG'CC
Cuts at: 4720 4720
Size: 8710
FspI TGC'GCA
Cuts at: 3856 5714 7216 3856
Size: 1858 1502 5350
Fragments arranged by size:
5350 1858 1502
FspAI rTGC'GCAy
Cuts at: 5714 5714
Size: 8710
HaeII r_GCGC'y
Cuts at: 1678 3804 4299 4896 5913 7857 1678
Size: 2126 495 597 1017 1944 2531
Fragments arranged by size:
2531 2126 1944 1017 597 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2257 2261 2272
Size: 32 135 32 574 32 79 4 11
Cuts at: 2272 2289 2293 2304 4550 4582 5847 5879 7351
Size: 17 4 11 2246 32 1265 32 1472
Cuts at: 7351 7383 7425 7457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2246 1472 1265 574 135 79 42
32 32 32 32 32 32 32 17
11 11 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 3163 3634 6054 6300 669
Size: 988 281 547 678 471 2420 246 3079
Fragments arranged by size:
3079 2420 988 678 547 471 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8710
HpaI GTT'AAC
Cuts at: 6300 6300
Size: 8710
KasI G'GCGC_C
Cuts at: 5909 5909
Size: 8710
KpnI G_GTAC'C
Cuts at: 6035 6035
Size: 8710
MfeI C'AATT_G
Cuts at: 6309 6309
Size: 8710
MluI A'CGCG_T
Cuts at: 8586 8586
Size: 8710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3855 7824 8008 1305
Size: 2550 3969 184 2007
Fragments arranged by size:
3969 2550 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2460 3737 3938 4493 4682
Size: 54 1425 343 628 1277 201 555 189
Cuts at: 4682 5238 10
Size: 556 3482
Fragments arranged by size:
3482 1425 1277 628 556 555 343 201
189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 3125 3272 3881 5219 5981 6668
Size: 648 1288 671 147 609 1338 762 687
Cuts at: 6668 7027 7186 518
Size: 359 159 2042
Fragments arranged by size:
2042 1338 1288 762 687 671 648 609
359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2641 2731 3181 3241 3301 3590 3852 3860
Size: 1035 90 450 60 60 289 262 8
Cuts at: 3860 3955 4739 5505 5853 5902 5919 6126 6803
Size: 95 784 766 348 49 17 207 677
Cuts at: 6803 7951 8196 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1035 784 766 677 450 348
289 262 245 207 95 90 60 60
49 17 8
NaeI GCC'GGC
Cuts at: 4718 4718
Size: 8710
NarI GG'CG_CC
Cuts at: 5910 5910
Size: 8710
NcoI C'CATG_G
Cuts at: 513 1065 2004 3267 3975 5220 513
Size: 552 939 1263 708 1245 4003
Fragments arranged by size:
4003 1263 1245 939 708 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8710
NgoMIV G'CCGG_C
Cuts at: 4716 4716
Size: 8710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8710
NotI GC'GGCC_GC
Cuts at: 6157 6157
Size: 8710
NspI r_CATG'y
Cuts at: 1132 1229 2845 3130 3586 5359 5814 6068 7613
Size: 97 1616 285 456 1773 455 254 1545
Cuts at: 7613 1132
Size: 2229
Fragments arranged by size:
2229 1773 1616 1545 456 455 285 254
97
PasI CC'CwG_GG
Cuts at: 1348 2929 3387 4309 1348
Size: 1581 458 922 5749
Fragments arranged by size:
5749 1581 922 458
PciI A'CATG_T
Cuts at: 7609 7609
Size: 8710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2976 3733 3934 1108
Size: 1868 757 201 5884
Fragments arranged by size:
5884 1868 757 201
PfoI T'CCnGG_A
Cuts at: 4554 4554
Size: 8710
PmeI GTTT'AAAC
Cuts at: 6014 6014
Size: 8710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 4961 4993 6779 6811 8292 8324 3755
Size: 32 1174 32 1786 32 1481 32 4141
Fragments arranged by size:
4141 1786 1481 1174 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2285 3151 3383 2285
Size: 866 232 7612
Fragments arranged by size:
7612 866 232
PshAI GACnn'nnGTC
Cuts at: 1613 3168 3381 1613
Size: 1555 213 6942
Fragments arranged by size:
6942 1555 213
PsiI TTA'TAA
Cuts at: 6280 6280
Size: 8710
PspOMI G'GGCC_C
Cuts at: 2352 4801 2352
Size: 2449 6261
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5186 5218 5186
Size: 32 8678
PstI C_TGCA'G
Cuts at: 830 2553 3318 3898 4056 5664 6062 830
Size: 1723 765 580 158 1608 398 3478
Fragments arranged by size:
3478 1723 1608 765 580 398 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8710
PvuII CAG'CTG
Cuts at: 2731 3181 3241 3301 3590 3955 5505 5919 2731
Size: 450 60 60 289 365 1550 414 5522
Fragments arranged by size:
5522 1550 450 414 365 289 60 60
SacI G_AGCT'C
Cuts at: 3452 4169 5426 5862 3452
Size: 717 1257 436 6300
Fragments arranged by size:
6300 1257 717 436
SalI G'TCGA_C
Cuts at: 1936 6052 1936
Size: 4116 4594
SanDI GG'GwC_CC
Cuts at: 3383 3383
Size: 8710
SapI GCTCTTCn'nnn_
Cuts at: 3017 4926 3017
Size: 1909 6801
SbfI CC_TGCA'GG
Cuts at: 3318 6062 3318
Size: 2744 5966
ScaI AGT'ACT
Cuts at: 3554 4503 5879 6958 8670 3554
Size: 949 1376 1079 1712 3594
Fragments arranged by size:
3594 1712 1376 1079 949
SfcI C'TryA_G
Cuts at: 826 1045 2549 2636 3314 3817 3894 4052 5121
Size: 219 1504 87 678 503 77 158 1069
Cuts at: 5121 5660 6058 7193 7874 8065 826
Size: 539 398 1135 681 191 1471
Fragments arranged by size:
1504 1471 1135 1069 681 678 539 503
398 219 191 158 87 77
SfoI GGC'GCC
Cuts at: 5911 5911
Size: 8710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8710
SmaI CCC'GGG
Cuts at: 1872 2993 6037 1872
Size: 1121 3044 4545
Fragments arranged by size:
4545 3044 1121
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6814 7715 7977 820
Size: 197 926 4871 901 262 1553
Fragments arranged by size:
4871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6501 493
Size: 6008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8710
SphI G_CATG'C
Cuts at: 1132 2845 6068 1132
Size: 1713 3223 3774
Fragments arranged by size:
3774 3223 1713
SspI AAT'ATT
Cuts at: 5 52 5055 6634 5
Size: 47 5003 1579 2081
Fragments arranged by size:
5003 2081 1579 47
StuI AGG'CCT
Cuts at: 1517 5654 1517
Size: 4137 4573
StyI C'CwwG_G
Cuts at: 513 1065 2004 2348 2427 3267 3871 3975 5220
Size: 552 939 344 79 840 604 104 1245
Cuts at: 5220 6149 513
Size: 929 3074
Fragments arranged by size:
3074 1245 939 929 840 604 552 344
104 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2921 3553 3721 5632 6737 6754 6907
Size: 1255 1341 632 168 1911 1105 17 153
Cuts at: 6907 7092 7537 325
Size: 185 445 1498
Fragments arranged by size:
1911 1498 1341 1255 1105 632 445 185
168 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 3552 4501
Size: 274 80 33 51 1443 546 1029 949
Cuts at: 4501 4749 5877 5985 6956 8668 96
Size: 248 1128 108 971 1712 138
Fragments arranged by size:
1712 1443 1128 1029 971 949 546 274
248 138 108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 4955 4987 8670 8702 3500
Size: 32 1423 32 3683 32 3508
Fragments arranged by size:
3683 3508 1423 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8710
XbaI T'CTAG_A
Cuts at: 6046 6046
Size: 8710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4306 5920 4306
Size: 1614 7096
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8710
XmaI C'CCGG_G
Cuts at: 1870 2991 6035 1870
Size: 1121 3044 4545
Fragments arranged by size:
4545 3044 1121
XmnI GAAnn'nnTTC
Cuts at: 4790 6839 4790
Size: 2049 6661
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6518 276
Size: 53 83 186 936 4984 2468
Fragments arranged by size:
4984 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I
EcoRV NruI NsiI PacI PmlI PspXI RsrII SacII
SexAI SfiI SrfI SwaI