Restriction map for
pFN21AE2320
(Circular) (Six-base) MAPSORT of: pFN21AE2320.seq Check: 2505 from: 1 to: 6463
pFN21AE2320 6463 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4273 278
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3806 1937
Size: 475 1394 4594
Fragments arranged by size:
4594 1394 475
Acc65I G'GTAC_C
Cuts at: 2584 3784 2584
Size: 1200 5263
AclI AA'CG_TT
Cuts at: 1563 4590 4963 1563
Size: 3027 373 3063
Fragments arranged by size:
3063 3027 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 3047 4526 5910 6178 1632
Size: 704 711 1479 1384 268 1917
Fragments arranged by size:
1917 1479 1384 711 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6266
AflIII A'CryG_T
Cuts at: 1203 2256 5362 6339 1203
Size: 1053 3106 977 1327
Fragments arranged by size:
3106 1327 1053 977
AgeI A'CCGG_T
Cuts at: 5294 5294
Size: 6463
AhdI GACnn_n'nnGTC
Cuts at: 5192 5192
Size: 6463
AleI CACnn'nnGTG
Cuts at: 1166 2438 1166
Size: 1272 5191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2362 3266 1184
Size: 1178 904 4381
Fragments arranged by size:
4381 1178 904
AlwNI CAG_nnn'CTG
Cuts at: 3430 4206 5778 3430
Size: 776 1572 4115
Fragments arranged by size:
4115 1572 776
ApaLI G'TGCA_C
Cuts at: 4520 5676 4520
Size: 1156 5307
ApoI r'AATT_y
Cuts at: 1493 2477 2615 3548 3772 3998 4150 6259 1493
Size: 984 138 933 224 226 152 2109 1697
Fragments arranged by size:
2109 1697 984 933 226 224 152 138
AseI AT'TA_AT
Cuts at: 160 1032 5017 160
Size: 872 3985 1606
Fragments arranged by size:
3985 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
AvaI C'yCGr_G
Cuts at: 1870 1943 3621 3684 3788 1870
Size: 73 1678 63 104 4545
Fragments arranged by size:
4545 1678 104 73 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3332 3365 3332
Size: 33 6430
BamHI G'GATC_C
Cuts at: 3793 4160 3793
Size: 367 6096
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 2451 2584 3242 3784
Size: 325 217 627 368 296 133 658 542
Cuts at: 3784 5239 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 658 627 542 368 325 296
217 133
BanII G_rGCy'C
Cuts at: 1391 2070 3611 3782 1391
Size: 679 1541 171 4072
Fragments arranged by size:
4072 1541 679 171
BbeI G_GCGC'C
Cuts at: 2159 2159
Size: 6463
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 928
Size: 971 676 4816
Fragments arranged by size:
4816 971 676
BbvCI CC'TCA_GC
Cuts at: 3006 3174 3006
Size: 168 6295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4654 4688 1903
Size: 34 2717 34 3678
Fragments arranged by size:
3678 2717 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2885 3360 4355 5565 2885
Size: 475 995 1210 3783
Fragments arranged by size:
3783 1210 995 475
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 5074 136
Size: 107 122 71 631 605 616 2786 1525
Fragments arranged by size:
2786 1525 631 616 605 122 107 71
BglII A'GATC_T
Cuts at: 6458 6458
Size: 6463
BlpI GC'TnA_GC
Cuts at: 2424 2468 3880 2424
Size: 44 1412 5007
Fragments arranged by size:
5007 1412 44
Bme1580I G_kGCm'C
Cuts at: 1790 2456 3245 4524 5680 1790
Size: 666 789 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 789 666
BmgBI CAC'GTC
Cuts at: 1318 2257 2431 1318
Size: 939 174 5350
Fragments arranged by size:
5350 939 174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2536 4194 5152
Size: 416 53 83 399 24 1119 1658 958
Cuts at: 5152 442
Size: 1753
Fragments arranged by size:
1753 1658 1119 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 6463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3282 3595 5123 1239
Size: 2043 313 1528 2579
Fragments arranged by size:
2579 2043 1528 313
Bpu10I CC'TnA_GC
Cuts at: 3006 3174 3492 3006
Size: 168 318 5977
Fragments arranged by size:
5977 318 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4588 5453 5751 4588
Size: 865 298 5300
Fragments arranged by size:
5300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3611 5126 882
Size: 617 2112 1515 2219
Fragments arranged by size:
2219 2112 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 4254 493
Size: 711 3050 2702
Fragments arranged by size:
3050 2702 711
BsaBI GATnn'nnATC
Cuts at: 6457 6457
Size: 6463
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2156 4270 4652 5287
Size: 53 83 186 936 623 2114 382 635
Cuts at: 5287 275
Size: 1451
Fragments arranged by size:
2114 1451 936 635 623 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4896 5294 5568 5715 6126 4896
Size: 398 274 147 411 5233
Fragments arranged by size:
5233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2362 2392 3536 3566 1178
Size: 30 165 30 959 30 1144 30 4075
Fragments arranged by size:
4075 1144 959 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2370 3544 3767 1186
Size: 909 275 1174 223 3882
Fragments arranged by size:
3882 1174 909 275 223
BseYI C'CCAG_C
Cuts at: 2177 5666 6162 2177
Size: 3489 496 2478
Fragments arranged by size:
3489 2478 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3316 1163
Size: 573 1580 4310
Fragments arranged by size:
4310 1580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3913 4674 5702 1540
Size: 275 186 1912 761 1028 2301
Fragments arranged by size:
2301 1912 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2279 2430 4524 4609 5680 2279
Size: 151 2094 85 1071 3062
Fragments arranged by size:
3062 2094 1071 151 85
BsmI GAATG_Cn'
Cuts at: 3974 4067 3974
Size: 93 6370
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2279 2430 2456 3245 3611 3782
Size: 399 280 209 151 26 789 366 171
Cuts at: 3782 4524 4609 5680 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 789 742 399 366 280 209
171 151 85 26
BspEI T'CCGG_A
Cuts at: 6126 6126
Size: 6463
BspHI T'CATG_A
Cuts at: 4352 6082 4352
Size: 1730 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3818 844
Size: 2974 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4350 1394
Size: 429 2527 3507
Fragments arranged by size:
3507 2527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4958 5132 65
Size: 1183 3710 174 1396
Fragments arranged by size:
3710 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3534 5107 5294 1700
Size: 110 1724 1573 187 2869
Fragments arranged by size:
2869 1724 1573 187 110
BsrGI T'GTAC_A
Cuts at: 96 2631 96
Size: 2535 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6463
BssSI C'ACGA_G
Cuts at: 4523 5535 4523
Size: 1012 5451
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6463
BstBI TT'CG_AA
Cuts at: 5267 5267
Size: 6463
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6463
BstYI r'GATC_y
Cuts at: 1972 2554 2751 2903 3793 4160 4545 4562 6014
Size: 582 197 152 890 367 385 17 1452
Cuts at: 6014 6100 6112 6458 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 890 582 385 367 346 197
152 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2382 2382
Size: 6463
BtgI C'CryG_G
Cuts at: 513 1065 3462 513
Size: 552 2397 3514
Fragments arranged by size:
3514 2397 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5724
Fragments arranged by size:
5724 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 3592 3979 4773 4793 3328
Size: 264 387 794 20 4998
Fragments arranged by size:
4998 794 387 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6463
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6428
DraI TTT'AAA
Cuts at: 3767 3767
Size: 6463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5470 809
Size: 507 4154 1802
Fragments arranged by size:
4154 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2289 3910 4799
Size: 54 1425 50 275 18 459 1621 889
Cuts at: 4799 6428 8
Size: 1629 43
Fragments arranged by size:
1629 1621 1425 889 459 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3910 1537
Size: 275 2098 4090
Fragments arranged by size:
4090 2098 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2764 4393 1353
Size: 1411 1629 3423
Fragments arranged by size:
3423 1629 1411
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2798 3650 5050 5424 5570 387
Size: 1064 138 1209 852 1400 374 146 1280
Fragments arranged by size:
1400 1280 1209 1064 852 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 3047 3282 3595 4526 5123 5910
Size: 393 704 711 235 313 931 597 787
Cuts at: 5910 6178 1239
Size: 268 1524
Fragments arranged by size:
1524 931 787 711 704 597 393 313
268 235
EcoRI G'AATT_C
Cuts at: 2477 2615 3548 3772 4150 2477
Size: 138 933 224 378 4790
Fragments arranged by size:
4790 933 378 224 138
FspI TGC'GCA
Cuts at: 4969 4969
Size: 6463
HaeII r_GCGC'y
Cuts at: 1678 2159 2851 5610 1678
Size: 481 692 2759 2531
Fragments arranged by size:
2759 2531 692 481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2362 2379 2394
Size: 32 135 32 611 32 147 17 15
Cuts at: 2394 2411 2485 2517 3495 3527 3642 3674 5104
Size: 17 74 32 978 32 115 32 1430
Cuts at: 5104 5136 5178 5210 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1430 978 611 147 135 115 74
42 32 32 32 32 32 32 32
32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3807 4053 669
Size: 988 281 1869 246 3079
Fragments arranged by size:
3079 1869 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6463
HpaI GTT'AAC
Cuts at: 4053 4053
Size: 6463
KasI G'GCGC_C
Cuts at: 2155 2155
Size: 6463
KpnI G_GTAC'C
Cuts at: 2588 3788 2588
Size: 1200 5263
MfeI C'AATT_G
Cuts at: 3015 4062 3015
Size: 1047 5416
MluI A'CGCG_T
Cuts at: 6339 6339
Size: 6463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2641 5577 5761 1305
Size: 942 394 2936 184 2007
Fragments arranged by size:
2936 2007 942 394 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4641
Fragments arranged by size:
4641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 3233 3248 3254 4421 4780 4939
Size: 648 1272 795 15 6 1167 359 159
Cuts at: 4939 518
Size: 2042
Fragments arranged by size:
2042 1272 1167 795 648 359 159 15
6
MspA1I CmG'CkG
Cuts at: 1606 3879 4556 5704 5949 1606
Size: 2273 677 1148 245 2120
Fragments arranged by size:
2273 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2156 2156
Size: 6463
NcoI C'CATG_G
Cuts at: 513 1065 3462 513
Size: 552 2397 3514
Fragments arranged by size:
3514 2397 552
NdeI CA'TA_TG
Cuts at: 387 3097 387
Size: 2710 3753
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6463
NotI GC'GGCC_GC
Cuts at: 3910 3910
Size: 6463
NspI r_CATG'y
Cuts at: 1132 1229 2349 3253 3509 3821 5366 1132
Size: 97 1120 904 256 312 1545 2229
Fragments arranged by size:
2229 1545 1120 904 312 256 97
PasI CC'CwG_GG
Cuts at: 1348 3424 1348
Size: 2076 4387
PciI A'CATG_T
Cuts at: 5362 5362
Size: 6463
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3260 1108
Size: 2152 4311
PfoI T'CCnGG_A
Cuts at: 3525 3525
Size: 6463
PmeI GTTT'AAAC
Cuts at: 3767 3767
Size: 6463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2122 4532 4564 6045 6077 2090
Size: 32 2410 32 1481 32 2476
Fragments arranged by size:
2476 2410 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6463
PsiI TTA'TAA
Cuts at: 4033 4033
Size: 6463
PstI C_TGCA'G
Cuts at: 830 3815 830
Size: 2985 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6463
SalI G'TCGA_C
Cuts at: 1936 3805 1936
Size: 1869 4594
SbfI CC_TGCA'GG
Cuts at: 3815 3815
Size: 6463
ScaI AGT'ACT
Cuts at: 4711 6423 4711
Size: 1712 4751
SfcI C'TryA_G
Cuts at: 826 1045 3594 3811 4946 5627 5818 826
Size: 219 2549 217 1135 681 191 1471
Fragments arranged by size:
2549 1471 1135 681 219 217 191
SfoI GGC'GCC
Cuts at: 2157 2157
Size: 6463
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6463
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6463
SmaI CCC'GGG
Cuts at: 1872 3623 3790 1872
Size: 1751 167 4545
Fragments arranged by size:
4545 1751 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4567 5468 5730 820
Size: 197 926 2624 901 262 1553
Fragments arranged by size:
2624 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4254 493
Size: 3761 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6463
SphI G_CATG'C
Cuts at: 1132 3821 1132
Size: 2689 3774
SspI AAT'ATT
Cuts at: 5 52 4387 5
Size: 47 4335 2081
Fragments arranged by size:
4335 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6463
StyI C'CwwG_G
Cuts at: 513 1065 3462 3902 513
Size: 552 2397 440 3074
Fragments arranged by size:
3074 2397 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3260 3302 4490 4507 4660 4845 5290
Size: 1255 1680 42 1188 17 153 185 445
Cuts at: 5290 325
Size: 1498
Fragments arranged by size:
1680 1498 1255 1188 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2052 2631 2822
Size: 274 80 33 51 1443 75 579 191
Cuts at: 2822 4709 6421 96
Size: 1887 1712 138
Fragments arranged by size:
1887 1712 1443 579 274 191 138 80
75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2199 2231 2859 2891 3536 3568 6423 6455 2199
Size: 32 628 32 645 32 2855 32 2207
Fragments arranged by size:
2855 2207 645 628 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6463
XbaI T'CTAG_A
Cuts at: 3799 3799
Size: 6463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3469 2011
Size: 1458 5005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6463
XmaI C'CCGG_G
Cuts at: 1870 3621 3788 1870
Size: 1751 167 4545
Fragments arranged by size:
4545 1751 167
XmnI GAAnn'nnTTC
Cuts at: 3701 4592 3701
Size: 891 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4271 276
Size: 53 83 186 936 2737 2468
Fragments arranged by size:
2737 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI