Restriction map for
pFN21AE2318
(Circular) (Six-base) MAPSORT of: pFN21AE2318.seq Check: 3157 from: 1 to: 10645
pFN21AE2318 10645 bp
With 182 enzymes: SgfI *
June 13, 2011 13:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8455 278
Size: 53 83 186 936 6919 2468
Fragments arranged by size:
6919 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7988 1937
Size: 6051 4594
Acc65I G'GTAC_C
Cuts at: 5290 7966 5290
Size: 2676 7969
AclI AA'CG_TT
Cuts at: 1563 8772 9145 1563
Size: 7209 373 3063
Fragments arranged by size:
7209 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 2735 3348 3537 3685 4430 4927 5382
Size: 667 436 613 189 148 745 497 455
Cuts at: 5382 5421 7294 8708 10092 10360 1632
Size: 39 1873 1414 1384 268 1917
Fragments arranged by size:
1917 1873 1414 1384 745 667 613 497
455 436 268 189 148 39
AflII C'TTAA_G
Cuts at: 820 1017 3320 5914 820
Size: 197 2303 2594 5551
Fragments arranged by size:
5551 2594 2303 197
AflIII A'CryG_T
Cuts at: 1203 2493 4351 5253 6321 7056 7281 9544 10521
Size: 1290 1858 902 1068 735 225 2263 977
Cuts at: 10521 1203
Size: 1327
Fragments arranged by size:
2263 1858 1327 1290 1068 977 902 735
225
AgeI A'CCGG_T
Cuts at: 5150 9476 5150
Size: 4326 6319
AhdI GACnn_n'nnGTC
Cuts at: 5254 9374 5254
Size: 4120 6525
AleI CACnn'nnGTG
Cuts at: 1166 5151 1166
Size: 3985 6660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10645
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 7604 7636 2480
Size: 32 5092 32 5489
Fragments arranged by size:
5489 5092 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2036 2951 3374 3643 8388 9960 2036
Size: 915 423 269 4745 1572 2721
Fragments arranged by size:
4745 2721 1572 915 423 269
ApaI G_GGCC'C
Cuts at: 3826 3826
Size: 10645
ApaLI G'TGCA_C
Cuts at: 2422 5048 8702 9858 2422
Size: 2626 3654 1156 3209
Fragments arranged by size:
3654 3209 2626 1156
ApoI r'AATT_y
Cuts at: 1493 2385 2935 3296 3620 4178 4386 4612 7954
Size: 892 550 361 324 558 208 226 3342
Cuts at: 7954 8180 8332 10441 1493
Size: 226 152 2109 1697
Fragments arranged by size:
3342 2109 1697 892 558 550 361 324
226 226 208 152
AseI AT'TA_AT
Cuts at: 160 1032 9199 160
Size: 872 8167 1606
Fragments arranged by size:
8167 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10645
AvaI C'yCGr_G
Cuts at: 1870 1943 3539 3733 7970 1870
Size: 73 1596 194 4237 4545
Fragments arranged by size:
4545 4237 1596 194 73
AvrII C'CTAG_G
Cuts at: 2641 2641
Size: 10645
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3004 3037 3004
Size: 33 10612
BamHI G'GATC_C
Cuts at: 3535 4426 5356 5470 5559 7975 8342 3535
Size: 891 930 114 89 2416 367 5838
Fragments arranged by size:
5838 2416 930 891 367 114 89
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2729 2891 4622 5290 5642
Size: 325 217 627 942 162 1731 668 352
Cuts at: 5642 5873 5969 6902 7966 9421 618
Size: 231 96 933 1064 1455 1842
Fragments arranged by size:
1842 1731 1455 1064 942 933 668 627
352 325 231 217 162 96
BanII G_rGCy'C
Cuts at: 1391 3422 3434 3740 3826 4940 6494 7491 7964
Size: 2031 12 306 86 1114 1554 997 473
Cuts at: 7964 1391
Size: 4072
Fragments arranged by size:
4072 2031 1554 1114 997 473 306 86
12
BbeI G_GCGC'C
Cuts at: 5646 5973 6906 5646
Size: 327 933 9385
Fragments arranged by size:
9385 933 327
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2723 4549 4654 5710 928
Size: 971 824 1826 105 1056 5863
Fragments arranged by size:
5863 1826 1056 971 824 105
BbvCI CC'TCA_GC
Cuts at: 7114 7114
Size: 10645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4785 4819 7379 7413 8836 8870 1903
Size: 34 2848 34 2560 34 1423 34 3678
Fragments arranged by size:
3678 2848 2560 1423 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3005 6203 8537 9747 3005
Size: 3198 2334 1210 3903
Fragments arranged by size:
3903 3198 2334 1210
BclI T'GATC_A
Cuts at: 1548 2412 6894 1548
Size: 864 4482 5299
Fragments arranged by size:
5299 4482 864
BfrBI ATG'CAT
Cuts at: 4762 4762
Size: 10645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3086 9256 136
Size: 107 122 71 631 605 1414 6170 1525
Fragments arranged by size:
6170 1525 1414 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4998 7374 10640 4998
Size: 2376 3266 5003
Fragments arranged by size:
5003 3266 2376
BlpI GC'TnA_GC
Cuts at: 3647 5633 8062 3647
Size: 1986 2429 6230
Fragments arranged by size:
6230 2429 1986
Bme1580I G_kGCm'C
Cuts at: 1790 2426 2734 3826 4163 4627 5052 7470 8706
Size: 636 308 1092 337 464 425 2418 1236
Cuts at: 8706 9862 1790
Size: 1156 2573
Fragments arranged by size:
2573 2418 1236 1156 1092 636 464 425
337 308
BmgBI CAC'GTC
Cuts at: 1318 4513 1318
Size: 3195 7450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7399 8376 9334
Size: 416 53 83 399 24 5982 977 958
Cuts at: 9334 442
Size: 1753
Fragments arranged by size:
5982 1753 977 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 3290 4979 5759 7322 7799 9305 1239
Size: 1028 1023 1689 780 1563 477 1506 2579
Fragments arranged by size:
2579 1689 1563 1506 1028 1023 780 477
Bpu10I CC'TnA_GC
Cuts at: 4934 7114 4934
Size: 2180 8465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2090 5735 8770 9635 9933 2090
Size: 3645 3035 865 298 2802
Fragments arranged by size:
3645 3035 2802 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3723 4814 5600 5902 7355 9308 882
Size: 617 2224 1091 786 302 1453 1953 2219
Fragments arranged by size:
2224 2219 1953 1453 1091 786 617 302
BsaAI yAC'GTr
Cuts at: 493 1204 8436 493
Size: 711 7232 2702
Fragments arranged by size:
7232 2702 711
BsaBI GATnn'nnATC
Cuts at: 5012 6254 6596 10639 5012
Size: 1242 342 4043 5018
Fragments arranged by size:
5018 4043 1242 342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 5643 5970 6903
Size: 53 83 186 936 2455 1655 327 933
Cuts at: 6903 8452 8834 9469 275
Size: 1549 382 635 1451
Fragments arranged by size:
2455 1655 1549 1451 936 933 635 382
327 186 83 53
BsaWI w'CCGG_w
Cuts at: 5150 5166 9078 9476 9750 9897 10308 5150
Size: 16 3912 398 274 147 411 5487
Fragments arranged by size:
5487 3912 411 398 274 147 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2480 2510 3722 3752 5378
Size: 30 165 30 1077 30 1212 30 1626
Cuts at: 5378 5408 1178
Size: 30 6415
Fragments arranged by size:
6415 1626 1212 1077 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3287 3539 3794 3871 5386 7703 1186
Size: 2101 252 255 77 1515 2317 4128
Fragments arranged by size:
4128 2317 2101 1515 255 252 77
BseYI C'CCAG_C
Cuts at: 2993 3075 3896 6487 9848 10344 2993
Size: 82 821 2591 3361 496 3294
Fragments arranged by size:
3361 3294 2591 821 496 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4968 5175 7353 1163
Size: 573 3232 207 2178 4455
Fragments arranged by size:
4455 3232 2178 573 207
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8095 8856 9884 1540
Size: 275 186 6094 761 1028 2301
Fragments arranged by size:
6094 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2426 3434 3768 4326 4940 5052 5595 6717 7491
Size: 1008 334 558 614 112 543 1122 774
Cuts at: 7491 7817 8706 8791 9862 2426
Size: 326 889 85 1071 3209
Fragments arranged by size:
3209 1122 1071 1008 889 774 614 558
543 334 326 112 85
BsmI GAATG_Cn'
Cuts at: 5962 8156 8249 5962
Size: 2194 93 8358
Fragments arranged by size:
8358 2194 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3995 3995
Size: 10645
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2426 2734 3422 3434 3740 3768 3826
Size: 399 636 308 688 12 306 28 58
Cuts at: 3826 4163 4326 4627 4940 5052 5595 6494 6717
Size: 337 163 301 313 112 543 899 223
Cuts at: 6717 7470 7491 7817 7964 8706 8791 9862 1391
Size: 753 21 326 147 742 85 1071 2174
Fragments arranged by size:
2174 1071 899 753 742 688 636 543
399 337 326 313 308 306 301 223
163 147 112 85 58 28 21 12
BspEI T'CCGG_A
Cuts at: 10308 10308
Size: 10645
BspHI T'CATG_A
Cuts at: 2448 2610 3630 5622 5985 6444 6588 8534 10264
Size: 162 1020 1992 363 459 144 1946 1730
Cuts at: 10264 2448
Size: 2829
Fragments arranged by size:
2829 1992 1946 1730 1020 459 363 162
144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5582 8000 844
Size: 4738 2418 3489
Fragments arranged by size:
4738 3489 2418
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 7518 7618 8532 1394
Size: 429 2056 3639 100 914 3507
Fragments arranged by size:
3639 3507 2056 914 429 100
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 2535 2653 3005 3312 4323 5200
Size: 1183 840 447 118 352 307 1011 877
Cuts at: 5200 5295 7287 9140 9314 65
Size: 95 1992 1853 174 1396
Fragments arranged by size:
1992 1853 1396 1183 1011 877 840 447
352 307 174 118 95
BsrFI r'CCGG_y
Cuts at: 1700 1810 5150 9289 9476 1700
Size: 110 3340 4139 187 2869
Fragments arranged by size:
4139 3340 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2340 6324 7284 96
Size: 2244 3984 960 3457
Fragments arranged by size:
3984 3457 2244 960
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10645
BssSI C'ACGA_G
Cuts at: 7037 8705 9717 7037
Size: 1668 1012 7965
Fragments arranged by size:
7965 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3347 7092 7436 7533 794
Size: 2553 3745 344 97 3906
Fragments arranged by size:
3906 3745 2553 344 97
BstBI TT'CG_AA
Cuts at: 2836 9449 2836
Size: 6613 4032
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10645
BstXI CCAn_nnnn'nTGG
Cuts at: 2589 5171 5189 5302 7300 2589
Size: 2582 18 113 1998 5934
Fragments arranged by size:
5934 2582 1998 113 18
BstYI r'GATC_y
Cuts at: 1972 3535 4040 4426 4998 5356 5470 5559 7374
Size: 1563 505 386 572 358 114 89 1815
Cuts at: 7374 7501 7975 8342 8727 8744 10196 10282 10294
Size: 127 474 367 385 17 1452 86 12
Cuts at: 10294 10640 1972
Size: 346 1977
Fragments arranged by size:
1977 1815 1563 1452 572 505 474 386
385 367 358 346 127 114 89 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 5457 5888 5457
Size: 431 10214
BtgI C'CryG_G
Cuts at: 513 1065 2004 2582 3237 4056 4422 4674 513
Size: 552 939 578 655 819 366 252 6484
Fragments arranged by size:
6484 939 819 655 578 552 366 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3368 7477 487
Size: 683 56 2142 4109 3655
Fragments arranged by size:
4109 3655 2142 683 56
BtsI GCAGTG_nn'
Cuts at: 3641 5084 5644 7531 7670 7707 8161 8955 8975
Size: 1443 560 1887 139 37 454 794 20
Cuts at: 8975 3641
Size: 5311
Fragments arranged by size:
5311 1887 1443 794 560 454 139 37
20
ClaI AT'CG_AT
Cuts at: 1554 7050 1554
Size: 5496 5149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3996 4031 6606 6641 529
Size: 35 3432 35 2575 35 4533
Fragments arranged by size:
4533 3432 2575 35 35 35
DraI TTT'AAA
Cuts at: 3301 7949 3301
Size: 4648 5997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9652 809
Size: 507 8336 1802
Fragments arranged by size:
8336 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3234 3357 4566
Size: 54 1425 50 275 18 1404 123 1209
Cuts at: 4566 4650 8092 8981 10610 8
Size: 84 3442 889 1629 43
Fragments arranged by size:
3442 1629 1425 1404 1209 889 275 123
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8092 1537
Size: 275 6280 4090
Fragments arranged by size:
6280 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2318 2907 3330 3439 3488 3812 4137
Size: 833 132 589 423 109 49 324 325
Cuts at: 4137 5430 6983 7608 8575 1353
Size: 1293 1553 625 967 3423
Fragments arranged by size:
3423 1553 1293 967 833 625 589 423
325 324 132 109 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 9232 9606 9752 387
Size: 1064 138 2507 5136 374 146 1280
Fragments arranged by size:
5136 2507 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3432 4938 7489 3432
Size: 1506 2551 6588
Fragments arranged by size:
6588 2551 1506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2299 2735 3290 3348 3537 3685
Size: 393 635 32 436 555 58 189 148
Cuts at: 3685 4430 4927 4979 5382 5421 5759 7294 7322
Size: 745 497 52 403 39 338 1535 28
Cuts at: 7322 7799 8708 9305 10092 10360 1239
Size: 477 909 597 787 268 1524
Fragments arranged by size:
1535 1524 909 787 745 635 597 555
497 477 436 403 393 338 268 189
148 58 52 39 32 28
EcoNI CCTnn'n_nnAGG
Cuts at: 3998 7175 3998
Size: 3177 7468
EcoO109I rG'GnC_Cy
Cuts at: 2033 2578 3713 4034 5440 5892 7567 2033
Size: 545 1135 321 1406 452 1675 5111
Fragments arranged by size:
5111 1675 1406 1135 545 452 321
EcoRI G'AATT_C
Cuts at: 7954 8332 7954
Size: 378 10267
EcoRV GAT'ATC
Cuts at: 5419 5419
Size: 10645
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6205 6205
Size: 10645
FspI TGC'GCA
Cuts at: 9151 9151
Size: 10645
HaeII r_GCGC'y
Cuts at: 1678 3835 5646 5973 6906 9792 1678
Size: 2157 1811 327 933 2886 2531
Fragments arranged by size:
2886 2531 2157 1811 933 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3722 3754 4461 4493 5240
Size: 32 135 32 2150 32 707 32 747
Cuts at: 5240 5272 5378 5410 6622 6654 7507 7539 7679
Size: 32 106 32 1212 32 853 32 140
Cuts at: 7679 7711 9286 9318 9360 9392 1373
Size: 32 1575 32 42 32 2626
Fragments arranged by size:
2626 2150 1575 1212 853 747 707 140
135 106 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5527 6043 6115 7989 8235 669
Size: 988 281 3589 516 72 1874 246 3079
Fragments arranged by size:
3589 3079 1874 988 516 281 246 72
HindIII A'AGCT_T
Cuts at: 748 6137 748
Size: 5389 5256
HpaI GTT'AAC
Cuts at: 6043 8235 6043
Size: 2192 8453
KasI G'GCGC_C
Cuts at: 5642 5969 6902 5642
Size: 327 933 9385
Fragments arranged by size:
9385 933 327
KpnI G_GTAC'C
Cuts at: 5294 7970 5294
Size: 2676 7969
MfeI C'AATT_G
Cuts at: 2721 3134 8244 2721
Size: 413 5110 5122
Fragments arranged by size:
5122 5110 413
MluI A'CGCG_T
Cuts at: 10521 10521
Size: 10645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3291 4433 5334 7569 9759 9943 1305
Size: 1986 1142 901 2235 2190 184 2007
Fragments arranged by size:
2235 2190 2007 1986 1142 901 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 3359 4568 4652 10
Size: 54 1425 343 1404 123 1209 84 6003
Fragments arranged by size:
6003 1425 1404 1209 343 123 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 3191 4421 4761 4841 5151 5169
Size: 648 1446 579 1230 340 80 310 18
Cuts at: 5169 5300 5621 5990 6443 7496 8603 8962 9121
Size: 131 321 369 453 1053 1107 359 159
Cuts at: 9121 518
Size: 2042
Fragments arranged by size:
2042 1446 1230 1107 1053 648 579 453
369 359 340 321 310 159 131 80
18
MspA1I CmG'CkG
Cuts at: 1606 2818 3849 3858 4397 5208 5506 6269 7118
Size: 1212 1031 9 539 811 298 763 849
Cuts at: 7118 7882 8061 8738 9886 10131 1606
Size: 764 179 677 1148 245 2120
Fragments arranged by size:
2120 1212 1148 1031 849 811 764 763
677 539 298 245 179 9
NarI GG'CG_CC
Cuts at: 5643 5970 6903 5643
Size: 327 933 9385
Fragments arranged by size:
9385 933 327
NcoI C'CATG_G
Cuts at: 513 1065 2004 2582 3237 4056 4422 513
Size: 552 939 578 655 819 366 6736
Fragments arranged by size:
6736 939 819 655 578 552 366
NdeI CA'TA_TG
Cuts at: 387 4758 7027 387
Size: 4371 2269 4005
Fragments arranged by size:
4371 4005 2269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10645
NotI GC'GGCC_GC
Cuts at: 8092 8092
Size: 10645
NsiI A_TGCA'T
Cuts at: 4764 4764
Size: 10645
NspI r_CATG'y
Cuts at: 1132 1229 2959 4355 4594 5257 6325 7060 7285
Size: 97 1730 1396 239 663 1068 735 225
Cuts at: 7285 8003 9548 1132
Size: 718 1545 2229
Fragments arranged by size:
2229 1730 1545 1396 1068 735 718 663
239 225 97
PasI CC'CwG_GG
Cuts at: 1348 4627 5854 7187 1348
Size: 3279 1227 1333 4806
Fragments arranged by size:
4806 3279 1333 1227
PciI A'CATG_T
Cuts at: 4351 5253 6321 7056 7281 9544 4351
Size: 902 1068 735 225 2263 5452
Fragments arranged by size:
5452 2263 1068 902 735 225
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2230 4575 5793 6756 7193 7537 1108
Size: 1122 2345 1218 963 437 344 4216
Fragments arranged by size:
4216 2345 1218 1122 963 437 344
PfoI T'CCnGG_A
Cuts at: 5697 5736 6278 7299 5697
Size: 39 542 1021 9043
Fragments arranged by size:
9043 1021 542 39
PmeI GTTT'AAAC
Cuts at: 7949 7949
Size: 10645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2512 2955 2987 3734 3766 8714 8746 10227
Size: 32 443 32 747 32 4948 32 1481
Cuts at: 10227 10259 2480
Size: 32 2866
Fragments arranged by size:
4948 2866 1481 747 443 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 3713 5440 5892 7567 2578
Size: 1135 1727 452 1675 5656
Fragments arranged by size:
5656 1727 1675 1135 452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10645
PsiI TTA'TAA
Cuts at: 8215 8215
Size: 10645
PspOMI G'GGCC_C
Cuts at: 3822 3822
Size: 10645
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6650 6682 6650
Size: 32 10613
PstI C_TGCA'G
Cuts at: 830 2817 7169 7702 7713 7997 830
Size: 1987 4352 533 11 284 3478
Fragments arranged by size:
4352 3478 1987 533 284 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10645
PvuII CAG'CTG
Cuts at: 2818 6269 7118 2818
Size: 3451 849 6345
Fragments arranged by size:
6345 3451 849
SacI G_AGCT'C
Cuts at: 3434 4940 7491 3434
Size: 1506 2551 6588
Fragments arranged by size:
6588 2551 1506
SalI G'TCGA_C
Cuts at: 1936 7987 1936
Size: 6051 4594
SanDI GG'GwC_CC
Cuts at: 2578 2578
Size: 10645
SapI GCTCTTCn'nnn_
Cuts at: 3439 6983 7608 3439
Size: 3544 625 6476
Fragments arranged by size:
6476 3544 625
SbfI CC_TGCA'GG
Cuts at: 7997 7997
Size: 10645
ScaI AGT'ACT
Cuts at: 6467 7418 8893 10605 6467
Size: 951 1475 1712 6507
Fragments arranged by size:
6507 1712 1475 951
SexAI A'CCwGG_T
Cuts at: 6678 6678
Size: 10645
SfcI C'TryA_G
Cuts at: 826 1045 2813 3844 3853 3862 4362 6270 7165
Size: 219 1768 1031 9 9 500 1908 895
Cuts at: 7165 7698 7709 7824 7993 9128 9809 10000 826
Size: 533 11 115 169 1135 681 191 1471
Fragments arranged by size:
1908 1768 1471 1135 1031 895 681 533
500 219 191 169 115 11 9 9
SfoI GGC'GCC
Cuts at: 5644 5971 6904 5644
Size: 327 933 9385
Fragments arranged by size:
9385 933 327
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10645
SgrAI Cr'CCGG_yG
Cuts at: 1700 5150 1700
Size: 3450 7195
SmaI CCC'GGG
Cuts at: 1872 3735 7972 1872
Size: 1863 4237 4545
Fragments arranged by size:
4545 4237 1863
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 3320 5750 5914 8749 9650
Size: 197 926 162 1215 2430 164 2835 901
Cuts at: 9650 9912 820
Size: 262 1553
Fragments arranged by size:
2835 2430 1553 1215 926 901 262 197
164 162
SnaBI TAC'GTA
Cuts at: 493 8436 493
Size: 7943 2702
SpeI A'CTAG_T
Cuts at: 152 2524 4126 152
Size: 2372 1602 6671
Fragments arranged by size:
6671 2372 1602
SphI G_CATG'C
Cuts at: 1132 2959 8003 1132
Size: 1827 5044 3774
Fragments arranged by size:
5044 3774 1827
SspI AAT'ATT
Cuts at: 5 52 8569 5
Size: 47 8517 2081
Fragments arranged by size:
8517 2081 47
StuI AGG'CCT
Cuts at: 1517 2229 2712 3587 1517
Size: 712 483 875 8575
Fragments arranged by size:
8575 875 712 483
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 2582 2641 3237 4056 4422
Size: 552 939 220 358 59 596 819 366
Cuts at: 4422 5961 6538 7446 8084 513
Size: 1539 577 908 638 3074
Fragments arranged by size:
3074 1539 939 908 819 638 596 577
552 366 358 220 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 4610 6464 7200 7418 8672 8689
Size: 1255 1137 1893 1854 736 218 1254 17
Cuts at: 8689 8842 9027 9472 325
Size: 153 185 445 1498
Fragments arranged by size:
1893 1854 1498 1255 1254 1137 736 445
218 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 2490 5232
Size: 274 80 33 51 1443 363 150 2742
Cuts at: 5232 6324 6465 7284 7416 8891 10603 96
Size: 1092 141 819 132 1475 1712 138
Fragments arranged by size:
2742 1712 1475 1443 1092 819 363 274
150 141 138 132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4602 4634 10605 10637 4602
Size: 32 5971 32 4610
Fragments arranged by size:
5971 4610 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5714 1316
Size: 4398 6247
XbaI T'CTAG_A
Cuts at: 7981 7981
Size: 10645
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6484 7567 6484
Size: 1083 9562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10645
XmaI C'CCGG_G
Cuts at: 1870 3733 7970 1870
Size: 1863 4237 4545
Fragments arranged by size:
4545 4237 1863
XmnI GAAnn'nnTTC
Cuts at: 3749 6377 6422 6764 8774 3749
Size: 2628 45 342 2010 5620
Fragments arranged by size:
5620 2628 2010 342 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8453 276
Size: 53 83 186 936 6919 2468
Fragments arranged by size:
6919 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BplI BsiWI BstZ17I DraIII FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspXI RsrII
SacII SfiI SrfI SwaI