Restriction map for
pFN21AE2316
(Circular) (Six-base) MAPSORT of: pFN21AE2316.seq Check: 6021 from: 1 to: 5899
pFN21AE2316 5899 bp
With 182 enzymes: SgfI *
January 11, 2011 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2281 3242 1937
Size: 344 961 4594
Fragments arranged by size:
4594 961 344
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2892 2937 3962 5346 5614 1632
Size: 1260 45 1025 1384 268 1917
Fragments arranged by size:
1917 1384 1260 1025 268 45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 2793 4798 5775 1203
Size: 1590 2005 977 1327
Fragments arranged by size:
2005 1590 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 4628 4628
Size: 5899
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 2222 3642 5214 2222
Size: 1420 1572 2907
Fragments arranged by size:
2907 1572 1420
ApaI G_GGCC'C
Cuts at: 2210 2210
Size: 5899
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 2183 2497 3024 3037 3131 3208 3434 3586
Size: 690 314 527 13 94 77 226 152
Cuts at: 3586 5695 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 690 527 314 226 152 94
77 13
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 2947 3229 3596 2947
Size: 282 367 5250
Fragments arranged by size:
5250 367 282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2639 3220 4675 618
Size: 325 217 627 852 581 1455 1842
Fragments arranged by size:
1842 1455 852 627 581 325 217
BanII G_rGCy'C
Cuts at: 1391 2210 2829 3218 1391
Size: 819 619 389 4072
Fragments arranged by size:
4072 819 619 389
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BfrBI ATG'CAT
Cuts at: 2732 2732
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 2656 3316 2656
Size: 660 5239
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2642 2688 3960 5116 1790
Size: 420 432 46 1272 1156 2573
Fragments arranged by size:
2573 1272 1156 432 420 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 3630 4588
Size: 416 53 83 399 24 1715 498 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1715 958 498 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2320 3081 3176 3191 4559 1239
Size: 1081 761 95 15 1368 2579
Fragments arranged by size:
2579 1368 1081 761 95 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 2596 4024 4889 5187 2250
Size: 346 1428 865 298 2962
Fragments arranged by size:
2962 1428 865 346 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3690 493
Size: 711 2486 2702
Fragments arranged by size:
2702 2486 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4332 4730 5004 5151 5562 4332
Size: 398 274 147 411 4669
Fragments arranged by size:
4669 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5674
Fragments arranged by size:
5674 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3173 1186
Size: 1987 3912
BseYI C'CCAG_C
Cuts at: 2304 5102 5598 2304
Size: 2798 496 2605
Fragments arranged by size:
2798 2605 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5326
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2158 2829 3960 4045 5116 2158
Size: 671 1131 85 1071 2941
Fragments arranged by size:
2941 1131 1071 671 85
BsmI GAATG_Cn'
Cuts at: 2732 3410 3503 2732
Size: 678 93 5128
Fragments arranged by size:
5128 678 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2210 2642 2688 2829 3218 3960
Size: 399 368 52 432 46 141 389 742
Cuts at: 3960 4045 5116 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 389 368 141
85 52 46
BspEI T'CCGG_A
Cuts at: 5562 5562
Size: 5899
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3254 844
Size: 2410 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2905 4394 4568 65
Size: 1183 1657 1489 174 1396
Fragments arranged by size:
1657 1489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4543 4730 1700
Size: 110 2733 187 2869
Fragments arranged by size:
2869 2733 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 3078 3078
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 2094 2947 3229 3596 3981 3998 5450 5536
Size: 122 853 282 367 385 17 1452 86
Cuts at: 5536 5548 5894 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 853 385 367 346 282 122
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 3415 4209 4229 3415
Size: 794 20 5085
Fragments arranged by size:
5085 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3346 4235 5864
Size: 54 1425 50 275 18 1516 889 1629
Cuts at: 5864 8
Size: 43
Fragments arranged by size:
1629 1516 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2868 3829 1353
Size: 1515 961 3423
Fragments arranged by size:
3423 1515 961
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2827 2827
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2320 2892 2937 3081 3176 3191 3962
Size: 393 688 572 45 144 95 15 771
Cuts at: 3962 4559 5346 5614 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 771 688 597 572 393 268
144 95 45 15
EcoO109I rG'GnC_Cy
Cuts at: 2207 2207
Size: 5899
EcoRI G'AATT_C
Cuts at: 2497 3131 3208 3586 2497
Size: 634 77 378 4810
Fragments arranged by size:
4810 634 378 77
EcoRV GAT'ATC
Cuts at: 2548 2548
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 5046 1678
Size: 3368 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 4540 4572 4614
Size: 32 135 32 676 32 2260 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 2260 676 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2866 3243 3489 669
Size: 988 281 928 377 246 3079
Fragments arranged by size:
3079 988 928 377 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 2193 2427 3498 2193
Size: 234 1071 4594
Fragments arranged by size:
4594 1071 234
MluI A'CGCG_T
Cuts at: 2793 5775 2793
Size: 2982 2917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5013 5197 1305
Size: 3708 184 2007
Fragments arranged by size:
3708 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3857 4216 4375 518
Size: 648 2691 359 159 2042
Fragments arranged by size:
2691 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2591 3315 3992 5140 5385 1606
Size: 985 724 677 1148 245 2120
Fragments arranged by size:
2120 1148 985 724 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4408
Fragments arranged by size:
4408 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NsiI A_TGCA'T
Cuts at: 2734 2734
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2198 2395 1108
Size: 1090 197 4612
Fragments arranged by size:
4612 1090 197
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2206 2206
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2916 3251 830
Size: 2086 335 3478
Fragments arranged by size:
3478 2086 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2591 2591
Size: 5899
SacI G_AGCT'C
Cuts at: 2829 2829
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2278 2348 2912 3247 4382 5063 5254
Size: 219 1233 70 564 335 1135 681 191
Cuts at: 5254 826
Size: 1471
Fragments arranged by size:
1471 1233 1135 681 564 335 219 191
70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 2575 4003 4904 5166 820
Size: 197 926 322 310 1428 901 262 1553
Fragments arranged by size:
1553 1428 926 901 322 310 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 2602 1517
Size: 1085 4814
StyI C'CwwG_G
Cuts at: 513 1065 2004 2502 3338 513
Size: 552 939 498 836 3074
Fragments arranged by size:
3074 939 836 552 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2396 3926 3943 4096 4281 4726 325
Size: 1255 816 1530 17 153 185 445 1498
Fragments arranged by size:
1530 1498 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5859 5891 5859
Size: 32 5867
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 3057 4028 3057
Size: 971 4928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI