Restriction map for
pFN21AE2315
(Circular) (Six-base) MAPSORT of: pFN21AE2315.seq Check: 7123 from: 1 to: 8008
pFN21AE2315 8008 bp
With 182 enzymes: SgfI *
January 11, 2011 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4019 4019
Size: 8008
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5818 278
Size: 53 83 186 936 4282 2468
Fragments arranged by size:
4282 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3390 5351 1937
Size: 1453 1961 4594
Fragments arranged by size:
4594 1961 1453
Acc65I G'GTAC_C
Cuts at: 5329 5329
Size: 8008
AclI AA'CG_TT
Cuts at: 1563 4794 6135 6508 1563
Size: 3231 1341 373 3063
Fragments arranged by size:
3231 3063 1341 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4485 4657 5154 6071 7455 7723 1632
Size: 2853 172 497 917 1384 268 1917
Fragments arranged by size:
2853 1917 1384 917 497 268 172
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7811
AflIII A'CryG_T
Cuts at: 1203 2322 6907 7884 1203
Size: 1119 4585 977 1327
Fragments arranged by size:
4585 1327 1119 977
AgeI A'CCGG_T
Cuts at: 6839 6839
Size: 8008
AhdI GACnn_n'nnGTC
Cuts at: 6737 6737
Size: 8008
AleI CACnn'nnGTG
Cuts at: 1166 3936 3989 1166
Size: 2770 53 5185
Fragments arranged by size:
5185 2770 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8008
AlwNI CAG_nnn'CTG
Cuts at: 5751 7323 5751
Size: 1572 6436
ApaLI G'TGCA_C
Cuts at: 6065 7221 6065
Size: 1156 6852
ApoI r'AATT_y
Cuts at: 1493 2617 3043 3064 3800 4085 4250 4871 5317
Size: 1124 426 21 736 285 165 621 446
Cuts at: 5317 5543 5695 7804 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 736 621 446 426 285
226 165 152 21
AseI AT'TA_AT
Cuts at: 160 1032 2901 5033 6562 160
Size: 872 1869 2132 1529 1606
Fragments arranged by size:
2132 1869 1606 1529 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 4884 5333 1870
Size: 73 162 2779 449 4545
Fragments arranged by size:
4545 2779 449 162 73
BamHI G'GATC_C
Cuts at: 3691 5338 5705 3691
Size: 1647 367 5994
Fragments arranged by size:
5994 1647 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5329 6784 618
Size: 325 217 627 3542 1455 1842
Fragments arranged by size:
3542 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2112 4069 4984 5327 1391
Size: 715 6 1957 915 343 4072
Fragments arranged by size:
4072 1957 915 715 343 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3522 3644 928
Size: 971 1623 122 5292
Fragments arranged by size:
5292 1623 971 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6199 6233 1903
Size: 34 4262 34 3678
Fragments arranged by size:
4262 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5900 7110 4172
Size: 1728 1210 5070
Fragments arranged by size:
5070 1728 1210
BclI T'GATC_A
Cuts at: 1548 2647 3697 5155 1548
Size: 1099 1050 1458 4401
Fragments arranged by size:
4401 1458 1099 1050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6619 136
Size: 107 122 71 631 605 4947 1525
Fragments arranged by size:
4947 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8003 8003
Size: 8008
BlpI GC'TnA_GC
Cuts at: 5425 5425
Size: 8008
Bme1580I G_kGCm'C
Cuts at: 1790 6069 7225 1790
Size: 4279 1156 2573
Fragments arranged by size:
4279 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3650 4508 1318
Size: 2332 858 4818
Fragments arranged by size:
4818 2332 858
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5739 6697 442
Size: 416 53 83 399 24 4322 958 1753
Fragments arranged by size:
4322 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8008
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 8008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2586 5076 6668 1239
Size: 1347 2490 1592 2579
Fragments arranged by size:
2579 2490 1592 1347
Bpu10I CC'TnA_GC
Cuts at: 4080 4080
Size: 8008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2352 4979 6133 6998 7296 2352
Size: 2627 1154 865 298 3064
Fragments arranged by size:
3064 2627 1154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2289 2970 4838 6671 882
Size: 617 790 681 1868 1833 2219
Fragments arranged by size:
2219 1868 1833 790 681 617
BsaAI yAC'GTr
Cuts at: 493 1204 5799 493
Size: 711 4595 2702
Fragments arranged by size:
4595 2702 711
BsaBI GATnn'nnATC
Cuts at: 3696 8002 3696
Size: 4306 3702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5170 5815 6197 6832
Size: 53 83 186 936 3637 645 382 635
Cuts at: 6832 275
Size: 1451
Fragments arranged by size:
3637 1451 936 645 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6441 6839 7113 7260 7671 6441
Size: 398 274 147 411 6778
Fragments arranged by size:
6778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2779 2809 1178
Size: 30 165 30 1376 30 6377
Fragments arranged by size:
6377 1376 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2147 3986 4967 4976 1186
Size: 958 3 1839 981 9 4218
Fragments arranged by size:
4218 1839 981 958 9 3
BseYI C'CCAG_C
Cuts at: 2733 2963 7211 7707 2733
Size: 230 4248 496 3034
Fragments arranged by size:
4248 3034 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3994 5112 1163
Size: 573 2258 1118 4059
Fragments arranged by size:
4059 2258 1118 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2100 5458 6219 7247 1540
Size: 275 186 99 3358 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 186 99
BsiHKAI G_wGCw'C
Cuts at: 2106 3996 4069 5142 6069 6154 7225 2106
Size: 1890 73 1073 927 85 1071 2889
Fragments arranged by size:
2889 1890 1073 1071 927 85 73
BsmI GAATG_Cn'
Cuts at: 5519 5612 5519
Size: 93 7915
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2112 3996 4069 4984 5142 5327
Size: 399 316 6 1884 73 915 158 185
Cuts at: 5327 6069 6154 7225 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1884 1071 915 742 399 316 185
158 85 73 6
BspEI T'CCGG_A
Cuts at: 7671 7671
Size: 8008
BspHI T'CATG_A
Cuts at: 4332 5897 7627 4332
Size: 1565 1730 4713
Fragments arranged by size:
4713 1730 1565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4016 4019 5084 5363 844
Size: 3172 3 1065 279 3489
Fragments arranged by size:
3489 3172 1065 279 3
BsrBI CCG'CTC
Cuts at: 1394 1823 5895 1394
Size: 429 4072 3507
Fragments arranged by size:
4072 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3883 6503 6677 65
Size: 1183 2635 2620 174 1396
Fragments arranged by size:
2635 2620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 2182 6652 6839 1700
Size: 110 221 151 4470 187 2869
Fragments arranged by size:
4470 2869 221 187 151 110
BsrGI T'GTAC_A
Cuts at: 96 4554 96
Size: 4458 3550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8008
BssSI C'ACGA_G
Cuts at: 3104 6068 7080 3104
Size: 2964 1012 4032
Fragments arranged by size:
4032 2964 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3133 794
Size: 2339 5669
BstBI TT'CG_AA
Cuts at: 6812 6812
Size: 8008
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8008
BstYI r'GATC_y
Cuts at: 1972 3691 5338 5705 6090 6107 7559 7645 7657
Size: 1719 1647 367 385 17 1452 86 12
Cuts at: 7657 8003 1972
Size: 346 1977
Fragments arranged by size:
1977 1719 1647 1452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2169 3421 513
Size: 552 939 90 75 1252 5100
Fragments arranged by size:
5100 1252 939 552 90 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7269
Fragments arranged by size:
7269 683 56
BtsI GCAGTG_nn'
Cuts at: 5524 6318 6338 5524
Size: 794 20 7194
Fragments arranged by size:
7194 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7973
DraI TTT'AAA
Cuts at: 3900 4092 5312 3900
Size: 192 1220 6596
Fragments arranged by size:
6596 1220 192
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7015 809
Size: 507 5699 1802
Fragments arranged by size:
5699 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 5455 6344
Size: 54 1425 50 275 18 267 3358 889
Cuts at: 6344 7973 8
Size: 1629 43
Fragments arranged by size:
3358 1629 1425 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2097 5455 1537
Size: 275 285 3358 4090
Fragments arranged by size:
4090 3358 285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2635 2758 4045 4464 5232 5241 5938 1353
Size: 1282 123 1287 419 768 9 697 3423
Fragments arranged by size:
3423 1287 1282 768 697 419 123 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2018 2176 6595 6969 7115 387
Size: 1064 138 429 158 4419 374 146 1280
Fragments arranged by size:
4419 1280 1064 429 374 158 146 138
EcoICRI GAG'CTC
Cuts at: 2104 4067 2104
Size: 1963 6045
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2586 4485 4657 5076 5154 6071 6668
Size: 393 954 1899 172 419 78 917 597
Cuts at: 6668 7455 7723 1239
Size: 787 268 1524
Fragments arranged by size:
1899 1524 954 917 787 597 419 393
268 172 78
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 2891
Size: 8008
EcoRI G'AATT_C
Cuts at: 3043 4250 5317 5695 3043
Size: 1207 1067 378 5356
Fragments arranged by size:
5356 1207 1067 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3152 5280 3152
Size: 2128 5880
FspI TGC'GCA
Cuts at: 6514 6514
Size: 8008
HaeII r_GCGC'y
Cuts at: 1678 2088 7155 1678
Size: 410 5067 2531
Fragments arranged by size:
5067 2531 410
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2660 2692 2735 2767 2915
Size: 32 135 32 1088 32 43 32 148
Cuts at: 2915 2947 5140 5147 5172 5179 6649 6681 6723
Size: 32 2193 7 25 7 1470 32 42
Cuts at: 6723 6755 1373
Size: 32 2626
Fragments arranged by size:
2626 2193 1470 1088 148 135 43 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 3616 4036 4801 5352 5598
Size: 988 281 436 1242 420 765 551 246
Cuts at: 5598 669
Size: 3079
Fragments arranged by size:
3079 1242 988 765 551 436 420 281
246
HindIII A'AGCT_T
Cuts at: 748 2065 4738 748
Size: 1317 2673 4018
Fragments arranged by size:
4018 2673 1317
HpaI GTT'AAC
Cuts at: 5598 5598
Size: 8008
KpnI G_GTAC'C
Cuts at: 5333 5333
Size: 8008
MfeI C'AATT_G
Cuts at: 3180 5151 5607 3180
Size: 1971 456 5581
Fragments arranged by size:
5581 1971 456
MluI A'CGCG_T
Cuts at: 7884 7884
Size: 8008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3125 3299 7122 7306 1305
Size: 1820 174 3823 184 2007
Fragments arranged by size:
3823 2007 1820 184 174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6186
Fragments arranged by size:
6186 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3936 3989 4947 5966 6325 6484 518
Size: 648 2770 53 958 1019 359 159 2042
Fragments arranged by size:
2770 2042 1019 958 648 359 159 53
MspA1I CmG'CkG
Cuts at: 1606 2096 2171 5424 6101 7249 7494 1606
Size: 490 75 3253 677 1148 245 2120
Fragments arranged by size:
3253 2120 1148 677 490 245 75
NaeI GCC'GGC
Cuts at: 2033 2033
Size: 8008
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6517
Fragments arranged by size:
6517 939 552
NdeI CA'TA_TG
Cuts at: 387 3172 387
Size: 2785 5223
NgoMIV G'CCGG_C
Cuts at: 2031 2031
Size: 8008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8008
NotI GC'GGCC_GC
Cuts at: 5455 5455
Size: 8008
NspI r_CATG'y
Cuts at: 1132 1229 2275 2326 2423 5366 6911 1132
Size: 97 1046 51 97 2943 1545 2229
Fragments arranged by size:
2943 2229 1545 1046 97 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8008
PciI A'CATG_T
Cuts at: 2322 6907 2322
Size: 4585 3423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3089 4854 1108
Size: 1981 1765 4262
Fragments arranged by size:
4262 1981 1765
PmeI GTTT'AAAC
Cuts at: 5312 5312
Size: 8008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6077 6109 7590 7622 6077
Size: 32 1481 32 6463
Fragments arranged by size:
6463 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2943 1613
Size: 1330 6678
PsiI TTA'TAA
Cuts at: 3663 4458 5578 3663
Size: 795 1120 6093
Fragments arranged by size:
6093 1120 795
PspXI vC'TCGA_Gb
Cuts at: 2105 4884 2105
Size: 2779 5229
PstI C_TGCA'G
Cuts at: 830 5360 830
Size: 4530 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8008
SacI G_AGCT'C
Cuts at: 2106 4069 2106
Size: 1963 6045
SacII CC_GC'GG
Cuts at: 2097 2172 2097
Size: 75 7933
SalI G'TCGA_C
Cuts at: 1936 5350 1936
Size: 3414 4594
SbfI CC_TGCA'GG
Cuts at: 5360 5360
Size: 8008
ScaI AGT'ACT
Cuts at: 6256 7968 6256
Size: 1712 6296
SfcI C'TryA_G
Cuts at: 826 1045 2424 4527 5356 6491 7172 7363 826
Size: 219 1379 2103 829 1135 681 191 1471
Fragments arranged by size:
2103 1471 1379 1135 829 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
SgrAI Cr'CCGG_yG
Cuts at: 1700 2031 1700
Size: 331 7677
SmaI CCC'GGG
Cuts at: 1872 5335 1872
Size: 3463 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 2367 4884 4958 6112 7013
Size: 197 926 162 262 2517 74 1154 901
Cuts at: 7013 7275 820
Size: 262 1553
Fragments arranged by size:
2517 1553 1154 926 901 262 262 197
162 74
SnaBI TAC'GTA
Cuts at: 493 5799 493
Size: 5306 2702
SpeI A'CTAG_T
Cuts at: 152 2768 152
Size: 2616 5392
SphI G_CATG'C
Cuts at: 1132 5366 1132
Size: 4234 3774
SspI AAT'ATT
Cuts at: 5 52 2853 3545 3871 5932 5
Size: 47 2801 692 326 2061 2081
Fragments arranged by size:
2801 2081 2061 692 326 47
StuI AGG'CCT
Cuts at: 1517 3977 1517
Size: 2460 5548
StyI C'CwwG_G
Cuts at: 513 1065 2004 2233 2941 4257 5447 513
Size: 552 939 229 708 1316 1190 3074
Fragments arranged by size:
3074 1316 1190 939 708 552 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2633 6035 6052 6205 6390 6835 325
Size: 1255 1053 3402 17 153 185 445 1498
Fragments arranged by size:
3402 1498 1255 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4554 6254 7966
Size: 274 80 33 51 1443 2577 1700 1712
Cuts at: 7966 96
Size: 138
Fragments arranged by size:
2577 1712 1700 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4767 4799 7968 8000 4767
Size: 32 3169 32 4775
Fragments arranged by size:
4775 3169 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8008
XbaI T'CTAG_A
Cuts at: 2858 5344 2858
Size: 2486 5522
XhoI C'TCGA_G
Cuts at: 1943 2105 4884 1943
Size: 162 2779 5067
Fragments arranged by size:
5067 2779 162
XmaI C'CCGG_G
Cuts at: 1870 5333 1870
Size: 3463 4545
XmnI GAAnn'nnTTC
Cuts at: 2770 3726 4599 6137 2770
Size: 956 873 1538 4641
Fragments arranged by size:
4641 1538 956 873
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5816 276
Size: 53 83 186 936 4282 2468
Fragments arranged by size:
4282 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI