Restriction map for
pFN21AE2314
(Circular) (Six-base) MAPSORT of: pFN21AE2314.seq Check: 238 from: 1 to: 10849
pFN21AE2314 10849 bp
With 182 enzymes: SgfI *
January 31, 2011 13:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7671 7671
Size: 10849
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8659 278
Size: 53 83 186 936 7123 2468
Fragments arranged by size:
7123 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 7277 8192 1937
Size: 579 4761 915 4594
Fragments arranged by size:
4761 4594 915 579
Acc65I G'GTAC_C
Cuts at: 8170 8170
Size: 10849
AclI AA'CG_TT
Cuts at: 1563 8976 9349 1563
Size: 7413 373 3063
Fragments arranged by size:
7413 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2838 3267 3795 6582 7359 8912 10296
Size: 756 450 429 528 2787 777 1553 1384
Cuts at: 10296 10564 1632
Size: 268 1917
Fragments arranged by size:
2787 1917 1553 1384 777 756 528 450
429 268
AflII C'TTAA_G
Cuts at: 820 1017 6077 820
Size: 197 5060 5592
Fragments arranged by size:
5592 5060 197
AflIII A'CryG_T
Cuts at: 1203 3272 4730 9748 10725 1203
Size: 2069 1458 5018 977 1327
Fragments arranged by size:
5018 2069 1458 1327 977
AgeI A'CCGG_T
Cuts at: 9680 9680
Size: 10849
AhdI GACnn_n'nnGTC
Cuts at: 9578 9578
Size: 10849
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5129 1184
Size: 3945 6904
AlwNI CAG_nnn'CTG
Cuts at: 5726 7663 7812 8592 10164 5726
Size: 1937 149 780 1572 6411
Fragments arranged by size:
6411 1937 1572 780 149
ApaLI G'TGCA_C
Cuts at: 6975 8906 10062 6975
Size: 1931 1156 7762
Fragments arranged by size:
7762 1931 1156
ApoI r'AATT_y
Cuts at: 1493 3329 4055 4185 4651 5059 5134 5146 5158
Size: 1836 726 130 466 408 75 12 12
Cuts at: 5158 5281 5329 5866 6136 6158 6421 7120 7213
Size: 123 48 537 270 22 263 699 93
Cuts at: 7213 7325 7797 8158 8384 8536 10645 1493
Size: 112 472 361 226 152 2109 1697
Fragments arranged by size:
2109 1836 1697 726 699 537 472 466
408 361 270 263 226 152 130 123
112 93 75 48 22 12 12
AseI AT'TA_AT
Cuts at: 160 1032 4740 9403 160
Size: 872 3708 4663 1606
Fragments arranged by size:
4663 3708 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10849
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 3435 8174 1870
Size: 73 358 1134 4739 4545
Fragments arranged by size:
4739 4545 1134 358 73
BamHI G'GATC_C
Cuts at: 8179 8546 8179
Size: 367 10482
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2243 8170 9625 618
Size: 325 217 627 335 121 5927 1455 1842
Fragments arranged by size:
5927 1842 1455 627 335 325 217 121
BanII G_rGCy'C
Cuts at: 1391 2045 2108 3704 8168 1391
Size: 654 63 1596 4464 4072
Fragments arranged by size:
4464 4072 1596 654 63
BbeI G_GCGC'C
Cuts at: 2126 2247 2126
Size: 121 10728
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 3231 4686 6456 928
Size: 971 511 821 1455 1770 5321
Fragments arranged by size:
5321 1770 1455 971 821 511
BbvCI CC'TCA_GC
Cuts at: 8061 8061
Size: 10849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9040 9074 1903
Size: 34 7103 34 3678
Fragments arranged by size:
7103 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2492 7216 8741 9951 2492
Size: 4724 1525 1210 3390
Fragments arranged by size:
4724 3390 1525 1210
BclI T'GATC_A
Cuts at: 1548 4252 5389 5617 1548
Size: 2704 1137 228 6780
Fragments arranged by size:
6780 2704 1137 228
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9460 136
Size: 107 122 71 631 605 7788 1525
Fragments arranged by size:
7788 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8067 10844 8067
Size: 2777 8072
BlpI GC'TnA_GC
Cuts at: 2148 2196 8266 2148
Size: 48 6070 4731
Fragments arranged by size:
6070 4731 48
Bme1580I G_kGCm'C
Cuts at: 1790 6979 8910 10066 1790
Size: 5189 1931 1156 2573
Fragments arranged by size:
5189 2573 1931 1156
BmgBI CAC'GTC
Cuts at: 1318 2037 6938 1318
Size: 719 4901 5229
Fragments arranged by size:
5229 4901 719
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2267 2357 5369
Size: 416 53 83 399 24 850 90 3012
Cuts at: 5369 8580 9538 442
Size: 3211 958 1753
Fragments arranged by size:
3211 3012 1753 958 850 416 399 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2811 3602 7907 9509 1239
Size: 935 637 791 4305 1602 2579
Fragments arranged by size:
4305 2579 1602 935 791 637
Bpu10I CC'TnA_GC
Cuts at: 5040 8061 5040
Size: 3021 7828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2029 4107 4152 5075 8974 9839 10137 2029
Size: 2078 45 923 3899 865 298 2741
Fragments arranged by size:
3899 2741 2078 923 865 298 45
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3425 5527 7608 9512 882
Size: 617 1926 2102 2081 1904 2219
Fragments arranged by size:
2219 2102 2081 1926 1904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3269 3275 8640 493
Size: 711 2065 6 5365 2702
Fragments arranged by size:
5365 2702 2065 711 6
BsaBI GATnn'nnATC
Cuts at: 2480 4984 10843 2480
Size: 2504 5859 2486
Fragments arranged by size:
5859 2504 2486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 2244 8656 9038
Size: 53 83 186 936 590 121 6412 382
Cuts at: 9038 9673 275
Size: 635 1451
Fragments arranged by size:
6412 1451 936 635 590 382 186 121
83 53
BsaWI w'CCGG_w
Cuts at: 2084 2342 9282 9680 9954 10101 10512 2084
Size: 258 6940 398 274 147 411 2421
Fragments arranged by size:
6940 2421 411 398 274 258 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 10624
Fragments arranged by size:
10624 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2619 4986 6851 7043 7046 8078 1186
Size: 1433 2367 1865 192 3 1032 3957
Fragments arranged by size:
3957 2367 1865 1433 1032 192 3
BseYI C'CCAG_C
Cuts at: 7412 10052 10548 7412
Size: 2640 496 7713
Fragments arranged by size:
7713 2640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 2276 2569 5034 5100 5307 5964
Size: 573 479 61 293 2465 66 207 657
Cuts at: 5964 6960 1163
Size: 996 5052
Fragments arranged by size:
5052 2465 996 657 573 479 293 207
66 61
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8299 9060 10088 1540
Size: 275 186 6298 761 1028 2301
Fragments arranged by size:
6298 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6979 8910 8995 10066 6979
Size: 1931 85 1071 7762
Fragments arranged by size:
7762 1931 1071 85
BsiWI C'GTAC_G
Cuts at: 2187 2187
Size: 10849
BsmI GAATG_Cn'
Cuts at: 3403 4331 6478 8360 8453 3403
Size: 928 2147 1882 93 5799
Fragments arranged by size:
5799 2147 1882 928 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2108 3704 6979 8168 8910 8995
Size: 399 255 63 1596 3275 1189 742 85
Cuts at: 8995 10066 1391
Size: 1071 2174
Fragments arranged by size:
3275 2174 1596 1189 1071 742 399 255
85 63
BspEI T'CCGG_A
Cuts at: 2084 10512 2084
Size: 8428 2421
BspHI T'CATG_A
Cuts at: 2848 3231 5350 5653 7477 8738 10468 2848
Size: 383 2119 303 1824 1261 1730 3229
Fragments arranged by size:
3229 2119 1824 1730 1261 383 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2796 3844 7671 8204 844
Size: 1952 1048 3827 533 3489
Fragments arranged by size:
3827 3489 1952 1048 533
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2200 8736 1394
Size: 429 187 190 6536 3507
Fragments arranged by size:
6536 3507 429 190 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 9344 9518 65
Size: 1183 2867 5229 174 1396
Fragments arranged by size:
5229 2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9493 9680 1700
Size: 110 7683 187 2869
Fragments arranged by size:
7683 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2379 3715 5257 5629 5926 96
Size: 2283 1336 1542 372 297 5019
Fragments arranged by size:
5019 2283 1542 1336 372 297
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10849
BssSI C'ACGA_G
Cuts at: 5613 8909 9921 5613
Size: 3296 1012 6541
Fragments arranged by size:
6541 3296 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10849
BstBI TT'CG_AA
Cuts at: 4893 5831 9653 4893
Size: 938 3822 6089
Fragments arranged by size:
6089 3822 938
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10849
BstYI r'GATC_y
Cuts at: 1972 7136 7653 8067 8179 8546 8931 8948 10400
Size: 5164 517 414 112 367 385 17 1452
Cuts at: 10400 10486 10498 10844 1972
Size: 86 12 346 1977
Fragments arranged by size:
5164 1977 1452 517 414 385 367 346
112 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2577 3541 6318 2577
Size: 964 2777 7108
Fragments arranged by size:
7108 2777 964
BtgI C'CryG_G
Cuts at: 513 1065 2238 2370 513
Size: 552 1173 132 8992
Fragments arranged by size:
8992 1173 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7191 487
Size: 683 56 5965 4145
Fragments arranged by size:
5965 4145 683 56
BtsI GCAGTG_nn'
Cuts at: 6190 8365 9159 9179 6190
Size: 2175 794 20 7860
Fragments arranged by size:
7860 2175 794 20
ClaI AT'CG_AT
Cuts at: 1554 4264 1554
Size: 2710 8139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10814
DraI TTT'AAA
Cuts at: 4060 4563 5608 6309 6642 7119 7218 8153 4060
Size: 503 1045 701 333 477 99 935 6756
Fragments arranged by size:
6756 1045 935 701 503 477 333 99
DraIII CAC_nnn'GTG
Cuts at: 3938 5942 3938
Size: 2004 8845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9856 809
Size: 507 8540 1802
Fragments arranged by size:
8540 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2499 3214 3532
Size: 54 1425 50 275 18 669 715 318
Cuts at: 3532 3736 8296 9185 10814 8
Size: 204 4560 889 1629 43
Fragments arranged by size:
4560 1629 1425 889 715 669 318 275
204 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8296 1537
Size: 275 6484 4090
Fragments arranged by size:
6484 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3457 3692 4311 4893 6475 6828 8779 1353
Size: 2104 235 619 582 1582 353 1951 3423
Fragments arranged by size:
3423 2104 1951 1582 619 582 353 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4989 9436 9810 9956 387
Size: 1064 138 3400 4447 374 146 1280
Fragments arranged by size:
4447 3400 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2388 2811 2838 3267 3602 3795
Size: 393 542 214 423 27 429 335 193
Cuts at: 3795 6582 7359 7907 8912 9509 10296 10564 1239
Size: 2787 777 548 1005 597 787 268 1524
Fragments arranged by size:
2787 1524 1005 787 777 597 548 542
429 423 393 335 268 214 193 27
EcoO109I rG'GnC_Cy
Cuts at: 3856 3856
Size: 10849
EcoRI G'AATT_C
Cuts at: 4185 5134 5158 6136 7797 8158 8536 4185
Size: 949 24 978 1661 361 378 6498
Fragments arranged by size:
6498 1661 978 949 378 361 24
EcoRV GAT'ATC
Cuts at: 2980 2980
Size: 10849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 3689 4616 4919 5493 6572 2434
Size: 1255 927 303 574 1079 6711
Fragments arranged by size:
6711 1255 1079 927 574 303
FspI TGC'GCA
Cuts at: 7146 7440 9355 7146
Size: 294 1915 8640
Fragments arranged by size:
8640 1915 294
HaeII r_GCGC'y
Cuts at: 1678 2126 2247 7788 9996 1678
Size: 448 121 5541 2208 2531
Fragments arranged by size:
5541 2531 2208 448 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2685 2717 2830 2862 3429
Size: 32 135 32 1113 32 113 32 567
Cuts at: 3429 3461 4361 4393 4847 4879 6493 6525 9490
Size: 32 900 32 454 32 1614 32 2965
Cuts at: 9490 9522 9564 9596 1373
Size: 32 42 32 2626
Fragments arranged by size:
2965 2626 1614 1113 900 567 454 135
113 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7075 8193 8439 669
Size: 988 281 5137 1118 246 3079
Fragments arranged by size:
5137 3079 1118 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2523 2867 748
Size: 1775 344 8730
Fragments arranged by size:
8730 1775 344
HpaI GTT'AAC
Cuts at: 7075 8439 7075
Size: 1364 9485
KasI G'GCGC_C
Cuts at: 2122 2243 2122
Size: 121 10728
KpnI G_GTAC'C
Cuts at: 8174 8174
Size: 10849
MfeI C'AATT_G
Cuts at: 2266 8448 2266
Size: 6182 4667
MluI A'CGCG_T
Cuts at: 10725 10725
Size: 10849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 3331 3837 5317 5535 7913 9963 10147
Size: 834 1192 506 1480 218 2378 2050 184
Cuts at: 10147 1305
Size: 2007
Fragments arranged by size:
2378 2050 2007 1480 1192 834 506 218
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 9027
Fragments arranged by size:
9027 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3114 5167 7589 8102 8807 9166 9325
Size: 648 1948 2053 2422 513 705 359 159
Cuts at: 9325 518
Size: 2042
Fragments arranged by size:
2422 2053 2042 1948 705 648 513 359
159
MspA1I CmG'CkG
Cuts at: 1606 2141 2152 2240 2290 2394 2618 5129 8265
Size: 535 11 88 50 104 224 2511 3136
Cuts at: 8265 8942 10090 10335 1606
Size: 677 1148 245 2120
Fragments arranged by size:
3136 2511 2120 1148 677 535 245 224
104 88 50 11
NarI GG'CG_CC
Cuts at: 2123 2244 2123
Size: 121 10728
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 10297
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10849
NotI GC'GGCC_GC
Cuts at: 8296 8296
Size: 10849
NspI r_CATG'y
Cuts at: 1132 1229 4734 6524 8207 9752 1132
Size: 97 3505 1790 1683 1545 2229
Fragments arranged by size:
3505 2229 1790 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10849
PciI A'CATG_T
Cuts at: 4730 9748 4730
Size: 5018 5831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 2926 1108
Size: 1368 450 9031
Fragments arranged by size:
9031 1368 450
PmeI GTTT'AAAC
Cuts at: 8153 8153
Size: 10849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3453 3485 8918 8950 10431 10463 3453
Size: 32 5433 32 1481 32 3839
Fragments arranged by size:
5433 3839 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3856 3856
Size: 10849
PshAI GACnn'nnGTC
Cuts at: 1613 3527 1613
Size: 1914 8935
PsiI TTA'TAA
Cuts at: 3677 3798 4457 4842 4935 8419 3677
Size: 121 659 385 93 3484 6107
Fragments arranged by size:
6107 3484 659 385 121 93
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6719 6751 6719
Size: 32 10817
PstI C_TGCA'G
Cuts at: 830 7668 8201 830
Size: 6838 533 3478
Fragments arranged by size:
6838 3478 533
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10849
PvuII CAG'CTG
Cuts at: 2152 2290 5129 2152
Size: 138 2839 7872
Fragments arranged by size:
7872 2839 138
SacII CC_GC'GG
Cuts at: 2241 2241
Size: 10849
SalI G'TCGA_C
Cuts at: 1936 8191 1936
Size: 6255 4594
SapI GCTCTTCn'nnn_
Cuts at: 3692 4893 3692
Size: 1201 9648
SbfI CC_TGCA'GG
Cuts at: 8201 8201
Size: 10849
ScaI AGT'ACT
Cuts at: 3280 9097 10809 3280
Size: 5817 1712 3320
Fragments arranged by size:
5817 3320 1712
SexAI A'CCwGG_T
Cuts at: 2469 2746 2469
Size: 277 10572
SfcI C'TryA_G
Cuts at: 826 1045 3997 5713 7318 7664 8071 8197 9332
Size: 219 2952 1716 1605 346 407 126 1135
Cuts at: 9332 10013 10204 826
Size: 681 191 1471
Fragments arranged by size:
2952 1716 1605 1471 1135 681 407 346
219 191 126
SfoI GGC'GCC
Cuts at: 2124 2245 2124
Size: 121 10728
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10849
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10849
SmaI CCC'GGG
Cuts at: 1872 2303 8176 1872
Size: 431 5873 4545
Fragments arranged by size:
5873 4545 431
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 4122 4167 5054 6077 8953
Size: 197 926 101 2078 45 887 1023 2876
Cuts at: 8953 9854 10116 820
Size: 901 262 1553
Fragments arranged by size:
2876 2078 1553 1023 926 901 887 262
197 101 45
SnaBI TAC'GTA
Cuts at: 493 8640 493
Size: 8147 2702
SpeI A'CTAG_T
Cuts at: 152 2698 4805 5873 152
Size: 2546 2107 1068 5128
Fragments arranged by size:
5128 2546 2107 1068
SphI G_CATG'C
Cuts at: 1132 8207 1132
Size: 7075 3774
SspI AAT'ATT
Cuts at: 5 52 3049 4669 5224 5739 7066 8773 5
Size: 47 2997 1620 555 515 1327 1707 2081
Fragments arranged by size:
2997 2081 1707 1620 1327 555 515 47
StuI AGG'CCT
Cuts at: 1517 4284 1517
Size: 2767 8082
StyI C'CwwG_G
Cuts at: 513 1065 2118 2439 7152 8288 513
Size: 552 1053 321 4713 1136 3074
Fragments arranged by size:
4713 3074 1136 1053 552 321
SwaI ATTT'AAAT
Cuts at: 4060 4060
Size: 10849
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3629 8876 8893 9046 9231 9676 325
Size: 1255 2049 5247 17 153 185 445 1498
Fragments arranged by size:
5247 2049 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 3278 3715
Size: 274 80 33 51 1443 402 899 437
Cuts at: 3715 4733 5257 5629 5926 6017 6735 9095 10807
Size: 1018 524 372 297 91 718 2360 1712
Cuts at: 10807 96
Size: 138
Fragments arranged by size:
2360 1712 1443 1018 899 718 524 437
402 372 297 274 138 91 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 10809 10841 2531
Size: 32 8246 32 2539
Fragments arranged by size:
8246 2539 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2512 3196 6507 1316
Size: 1196 684 3311 5658
Fragments arranged by size:
5658 3311 1196 684
XbaI T'CTAG_A
Cuts at: 2212 2600 3250 5770 6166 8185 2212
Size: 388 650 2520 396 2019 4876
Fragments arranged by size:
4876 2520 2019 650 396 388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10849
XmaI C'CCGG_G
Cuts at: 1870 2301 8174 1870
Size: 431 5873 4545
Fragments arranged by size:
5873 4545 431
XmnI GAAnn'nnTTC
Cuts at: 7640 8978 7640
Size: 1338 9511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8657 276
Size: 53 83 186 936 7123 2468
Fragments arranged by size:
7123 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
RsrII SacI SanDI SfiI SrfI XcmI