Restriction map for
pFN21AE2312
(Circular) (Six-base) MAPSORT of: pFN21AE2312.seq Check: 96 from: 1 to: 11113
pFN21AE2312 11113 bp
With 182 enzymes: SgfI *
January 11, 2011 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5580 5580
Size: 11113
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8923 278
Size: 53 83 186 936 7387 2468
Fragments arranged by size:
7387 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2652 6021 6171 8456 1937
Size: 715 3369 150 2285 4594
Fragments arranged by size:
4594 3369 2285 715 150
Acc65I G'GTAC_C
Cuts at: 8434 8434
Size: 11113
AclI AA'CG_TT
Cuts at: 1563 5184 7921 9240 9613 1563
Size: 3621 2737 1319 373 3063
Fragments arranged by size:
3621 3063 2737 1319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4346 5039 5618 6272 6895 7043 7483
Size: 1742 972 693 579 654 623 148 440
Cuts at: 7483 7904 7979 9176 10560 10828 1632
Size: 421 75 1197 1384 268 1917
Fragments arranged by size:
1917 1742 1384 1197 972 693 654 623
579 440 421 268 148 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10916
AflIII A'CryG_T
Cuts at: 1203 4000 4224 6039 7986 10012 10989 1203
Size: 2797 224 1815 1947 2026 977 1327
Fragments arranged by size:
2797 2026 1947 1815 1327 977 224
AgeI A'CCGG_T
Cuts at: 9944 9944
Size: 11113
AhdI GACnn_n'nnGTC
Cuts at: 7357 9842 7357
Size: 2485 8628
AleI CACnn'nnGTG
Cuts at: 1166 4499 1166
Size: 3333 7780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3363 6353 7734 1184
Size: 2179 2990 1381 4563
Fragments arranged by size:
4563 2990 2179 1381
AlwNI CAG_nnn'CTG
Cuts at: 3229 4168 4301 4561 5017 7381 8153 8300 8856
Size: 939 133 260 456 2364 772 147 556
Cuts at: 8856 10428 3229
Size: 1572 3914
Fragments arranged by size:
3914 2364 1572 939 772 556 456 260
147 133
ApaI G_GGCC'C
Cuts at: 6055 6055
Size: 11113
ApaLI G'TGCA_C
Cuts at: 3012 5567 9170 10326 3012
Size: 2555 3603 1156 3799
Fragments arranged by size:
3799 3603 2555 1156
ApoI r'AATT_y
Cuts at: 1493 5443 5975 8366 8422 8648 8800 10909 1493
Size: 3950 532 2391 56 226 152 2109 1697
Fragments arranged by size:
3950 2391 2109 1697 532 226 152 56
AseI AT'TA_AT
Cuts at: 160 1032 9667 160
Size: 872 8635 1606
Fragments arranged by size:
8635 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11113
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2784 3773 8438 1870
Size: 73 388 453 989 4665 4545
Fragments arranged by size:
4665 4545 989 453 388 73
AvrII C'CTAG_G
Cuts at: 7447 7447
Size: 11113
BamHI G'GATC_C
Cuts at: 3302 3453 8443 8810 3302
Size: 151 4990 367 5605
Fragments arranged by size:
5605 4990 367 151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 3590 4493 5134 5865
Size: 325 217 627 363 1440 903 641 731
Cuts at: 5865 7435 7587 8303 8434 9889 618
Size: 1570 152 716 131 1455 1842
Fragments arranged by size:
1842 1570 1455 1440 903 731 716 641
627 363 325 217 152 131
BanII G_rGCy'C
Cuts at: 1391 4271 6055 6119 6876 7130 7497 8432 1391
Size: 2880 1784 64 757 254 367 935 4072
Fragments arranged by size:
4072 2880 1784 935 757 367 254 64
BbeI G_GCGC'C
Cuts at: 7439 7439
Size: 11113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2652 2891 3536 4582 4934 5047 5525
Size: 971 753 239 645 1046 352 113 478
Cuts at: 5525 6077 7602 928
Size: 552 1525 4439
Fragments arranged by size:
4439 1525 1046 971 753 645 552 478
352 239 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2044 2078 2804 2838 5338 5372 7396
Size: 34 107 34 726 34 2500 34 2024
Cuts at: 7396 7430 9304 9338 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 2500 2024 1874 726 107 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6991 9005 10215 6991
Size: 2014 1210 7889
Fragments arranged by size:
7889 2014 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11113
BfrBI ATG'CAT
Cuts at: 2798 7201 2798
Size: 4403 6710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9724 136
Size: 107 122 71 631 605 8052 1525
Fragments arranged by size:
8052 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3172 4867 11108 3172
Size: 1695 6241 3177
Fragments arranged by size:
6241 3177 1695
BlpI GC'TnA_GC
Cuts at: 4270 4302 6875 7811 8530 4270
Size: 32 2573 936 719 6853
Fragments arranged by size:
6853 2573 936 719 32
Bme1580I G_kGCm'C
Cuts at: 1790 2153 3016 5320 5571 6055 8308 9174 10330
Size: 363 863 2304 251 484 2253 866 1156
Cuts at: 10330 1790
Size: 2573
Fragments arranged by size:
2573 2304 2253 1156 866 863 484 363
251
BmgBI CAC'GTC
Cuts at: 1318 4568 7054 7432 7669 1318
Size: 3250 2486 378 237 4762
Fragments arranged by size:
4762 3250 2486 378 237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3723 4544 4576
Size: 416 53 83 399 24 2306 821 32
Cuts at: 4576 4964 5163 6997 8844 9802 442
Size: 388 199 1834 1847 958 1753
Fragments arranged by size:
2306 1847 1834 1753 958 821 416 399
388 199 83 53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7567 7567
Size: 11113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 5129 5147 6827 7412 8147 9773 1239
Size: 926 2964 18 1680 585 735 1626 2579
Fragments arranged by size:
2964 2579 1680 1626 926 735 585 18
Bpu10I CC'TnA_GC
Cuts at: 6916 6916
Size: 11113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3503 4817 5711 6815 9238 10103 10401 3503
Size: 1314 894 1104 2423 865 298 4215
Fragments arranged by size:
4215 2423 1314 1104 894 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 3959 4025 5834 6230 9776 882
Size: 617 831 1629 66 1809 396 3546 2219
Fragments arranged by size:
3546 2219 1809 1629 831 617 396 66
BsaAI yAC'GTr
Cuts at: 493 1204 2390 4001 8904 493
Size: 711 1186 1611 4903 2702
Fragments arranged by size:
4903 2702 1611 1186 711
BsaBI GATnn'nnATC
Cuts at: 3495 11107 3495
Size: 7612 3501
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6892 7436 8920 9302
Size: 53 83 186 936 5359 544 1484 382
Cuts at: 9302 9937 275
Size: 635 1451
Fragments arranged by size:
5359 1484 1451 936 635 544 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2091 5400 9546 9944 10218 10365 10776 2091
Size: 3309 4146 398 274 147 411 2428
Fragments arranged by size:
4146 3309 2428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4619 4649 6422 6452 1178
Size: 30 165 30 3216 30 1773 30 5839
Fragments arranged by size:
5839 3216 1773 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 4640 5111 5114 5714 6427 8150 1186
Size: 1459 1995 471 3 600 713 1723 4149
Fragments arranged by size:
4149 1995 1723 1459 713 600 471 3
BseYI C'CCAG_C
Cuts at: 2485 3422 3524 3822 4561 4762 5844 6259 6514
Size: 937 102 298 739 201 1082 415 255
Cuts at: 6514 6957 8293 10316 10812 2485
Size: 443 1336 2023 496 2786
Fragments arranged by size:
2786 2023 1336 1082 937 739 496 443
415 298 255 201 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2633 2701 3668 3751 4455 5018 5690
Size: 573 897 68 967 83 704 563 672
Cuts at: 5690 6102 6359 6563 6763 6765 8141 1163
Size: 412 257 204 200 2 1376 4135
Fragments arranged by size:
4135 1376 967 897 704 672 573 563
412 257 204 200 83 68 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8563 9324 10352 1540
Size: 275 186 6562 761 1028 2301
Fragments arranged by size:
6562 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3016 3514 4543 5571 5754 6119 7497 9174
Size: 949 498 1029 1028 183 365 1378 1677
Cuts at: 9174 9259 10330 2067
Size: 85 1071 2850
Fragments arranged by size:
2850 1677 1378 1071 1029 1028 949 498
365 183 85
BsmI GAATG_Cn'
Cuts at: 4744 4794 6108 6429 8624 8717 4744
Size: 50 1314 321 2195 93 7140
Fragments arranged by size:
7140 2195 1314 321 93 50
BsmBI CGTCTCn'nnnn_
Cuts at: 3479 6702 3479
Size: 3223 7890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2153 3016 3514 4271 4543 5320
Size: 399 277 86 863 498 757 272 777
Cuts at: 5320 5571 5754 6055 6119 6876 7130 7497 8308
Size: 251 183 301 64 757 254 367 811
Cuts at: 8308 8432 9174 9259 10330 1391
Size: 124 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 811 777 757 757 742
498 399 367 301 277 272 254 251
183 124 86 85 64
BspEI T'CCGG_A
Cuts at: 5400 10776 5400
Size: 5376 5737
BspHI T'CATG_A
Cuts at: 3094 4113 4822 8328 9002 10732 3094
Size: 1019 709 3506 674 1730 3475
Fragments arranged by size:
3506 3475 1730 1019 709 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2487 3214 5580 5922 6157 6870 7329 7689
Size: 1643 727 2366 342 235 713 459 360
Cuts at: 7689 8468 844
Size: 779 3489
Fragments arranged by size:
3489 2366 1643 779 727 713 459 360
342 235
BsrBI CCG'CTC
Cuts at: 1394 1823 6451 9000 1394
Size: 429 4628 2549 3507
Fragments arranged by size:
4628 3507 2549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3624 7166 7807 8157 9608 9782 65
Size: 1183 2376 3542 641 350 1451 174 1396
Fragments arranged by size:
3542 2376 1451 1396 1183 641 350 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 5271 9757 9944 1700
Size: 110 425 3036 4486 187 2869
Fragments arranged by size:
4486 3036 2869 425 187 110
BsrGI T'GTAC_A
Cuts at: 96 4503 6135 96
Size: 4407 1632 5074
Fragments arranged by size:
5074 4407 1632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11113
BssSI C'ACGA_G
Cuts at: 4391 9173 10185 4391
Size: 4782 1012 5319
Fragments arranged by size:
5319 4782 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5122 5923 6089 794
Size: 4328 801 166 5818
Fragments arranged by size:
5818 4328 801 166
BstBI TT'CG_AA
Cuts at: 9917 9917
Size: 11113
BstEII G'GTnAC_C
Cuts at: 1184 7849 1184
Size: 6665 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 3601 6502 3601
Size: 2901 8212
BstYI r'GATC_y
Cuts at: 1972 3112 3172 3187 3302 3453 4330 4399 4840
Size: 1140 60 15 115 151 877 69 441
Cuts at: 4840 4867 6790 7020 8443 8810 9195 9212 10664
Size: 27 1923 230 1423 367 385 17 1452
Cuts at: 10664 10750 10762 11108 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1923 1452 1423 1140 877 441 385
367 346 230 151 115 86 69 60
27 17 15 12
BstZ17I GTA'TAC
Cuts at: 2653 6022 6172 2653
Size: 3369 150 7594
Fragments arranged by size:
7594 3369 150
Bsu36I CC'TnA_GG
Cuts at: 2743 5412 7607 2743
Size: 2669 2195 6249
Fragments arranged by size:
6249 2669 2195
BtgI C'CryG_G
Cuts at: 513 1065 2004 3183 3218 6057 6147 6627 6882
Size: 552 939 1179 35 2839 90 480 255
Cuts at: 6882 513
Size: 4744
Fragments arranged by size:
4744 2839 1179 939 552 480 255 90
35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6331 487
Size: 683 56 5105 5269
Fragments arranged by size:
5269 5105 683 56
BtsI GCAGTG_nn'
Cuts at: 4313 6529 6717 6775 8101 8629 9423 9443 4313
Size: 2216 188 58 1326 528 794 20 5983
Fragments arranged by size:
5983 2216 1326 794 528 188 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 7844 7879 529
Size: 35 7280 35 3763
Fragments arranged by size:
7280 3763 35 35
DraI TTT'AAA
Cuts at: 8417 8417
Size: 11113
DraIII CAC_nnn'GTG
Cuts at: 3020 3020
Size: 11113
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2705 3951 10120 809
Size: 507 1389 1246 6169 1802
Fragments arranged by size:
6169 1802 1389 1246 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2889 3006 3273
Size: 54 1425 50 275 18 1059 117 267
Cuts at: 3273 3681 3900 5269 6630 8560 9449 11078 8
Size: 408 219 1369 1361 1930 889 1629 43
Fragments arranged by size:
1930 1629 1425 1369 1361 1059 889 408
275 267 219 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8560 1537
Size: 275 6748 4090
Fragments arranged by size:
6748 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2200 2664 3327 3732 6359 6484 6914 7502
Size: 847 464 663 405 2627 125 430 588
Cuts at: 7502 8174 8181 9043 1353
Size: 672 7 862 3423
Fragments arranged by size:
3423 2627 862 847 672 663 588 464
430 405 125 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 9700 10074 10220 387
Size: 1064 138 435 7676 374 146 1280
Fragments arranged by size:
7676 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 6117 7495 6117
Size: 1378 9735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 3374 4346 5039 5129 5147 5618
Size: 393 533 1209 972 693 90 18 471
Cuts at: 5618 6272 6827 6895 7043 7412 7483 7904 7979
Size: 654 555 68 148 369 71 421 75
Cuts at: 7979 8147 9176 9773 10560 10828 1239
Size: 168 1029 597 787 268 1524
Fragments arranged by size:
1524 1209 1029 972 787 693 654 597
555 533 471 421 393 369 268 168
148 90 75 71 68 18
EcoNI CCTnn'n_nnAGG
Cuts at: 4235 6866 7754 4235
Size: 2631 888 7594
Fragments arranged by size:
7594 2631 888
EcoO109I rG'GnC_Cy
Cuts at: 3132 3241 3878 6051 6052 6154 7627 8150 3132
Size: 109 637 2173 1 102 1473 523 6095
Fragments arranged by size:
6095 2173 1473 637 523 109 102 1
EcoRI G'AATT_C
Cuts at: 8422 8800 8422
Size: 378 10735
EcoRV GAT'ATC
Cuts at: 2395 2395
Size: 11113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4039 7159 7171 8299 4039
Size: 3120 12 1128 6853
Fragments arranged by size:
6853 3120 1128 12
FspI TGC'GCA
Cuts at: 9619 9619
Size: 11113
HaeII r_GCGC'y
Cuts at: 1678 3679 4861 6130 7108 7439 10260 1678
Size: 2001 1182 1269 978 331 2821 2531
Fragments arranged by size:
2821 2531 2001 1269 1182 978 331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 3116 3148 3867
Size: 32 135 32 539 32 973 32 719
Cuts at: 3867 3899 7343 7375 7524 7556 7715 7747 8204
Size: 32 3444 32 149 32 159 32 457
Cuts at: 8204 8236 9754 9786 9828 9860 1373
Size: 32 1518 32 42 32 2626
Fragments arranged by size:
3444 2626 1518 973 719 539 457 159
149 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4006 4381 8457 8703 669
Size: 988 281 2068 375 4076 246 3079
Fragments arranged by size:
4076 3079 2068 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 3891 4691 748
Size: 3143 800 7170
Fragments arranged by size:
7170 3143 800
HpaI GTT'AAC
Cuts at: 8703 8703
Size: 11113
KasI G'GCGC_C
Cuts at: 7435 7435
Size: 11113
KpnI G_GTAC'C
Cuts at: 8438 8438
Size: 11113
MfeI C'AATT_G
Cuts at: 8712 8712
Size: 11113
MluI A'CGCG_T
Cuts at: 7986 10989 7986
Size: 3003 8110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 3967 4505 5535 5921 6781 7329 8259
Size: 951 1711 538 1030 386 860 548 930
Cuts at: 8259 10227 10411 1305
Size: 1968 184 2007
Fragments arranged by size:
2007 1968 1711 1030 951 930 860 548
538 386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 3275 3683 3902 6632
Size: 54 1425 343 1176 267 408 219 2730
Cuts at: 6632 10
Size: 4491
Fragments arranged by size:
4491 2730 1425 1176 408 343 267 219
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2960 3581 3599 3923 4499 5087
Size: 648 990 804 621 18 324 576 588
Cuts at: 5087 5532 5810 6146 6552 6968 7029 7212 7964
Size: 445 278 336 406 416 61 183 752
Cuts at: 7964 9071 9430 9589 518
Size: 1107 359 159 2042
Fragments arranged by size:
2042 1107 990 804 752 648 621 588
576 445 416 406 359 336 324 278
183 159 61 18
MspA1I CmG'CkG
Cuts at: 1606 2411 2812 2920 3727 3826 4883 5014 5212
Size: 805 401 108 807 99 1057 131 198
Cuts at: 5212 5788 5826 5848 6066 7714 7891 7999 8125
Size: 576 38 22 218 1648 177 108 126
Cuts at: 8125 8529 9206 10354 10599 1606
Size: 404 677 1148 245 2120
Fragments arranged by size:
2120 1648 1148 1057 807 805 677 576
404 401 245 218 198 177 131 126
108 108 99 38 22
NarI GG'CG_CC
Cuts at: 7436 7436
Size: 11113
NcoI C'CATG_G
Cuts at: 513 1065 2004 3183 6147 6627 6882 513
Size: 552 939 1179 2964 480 255 4744
Fragments arranged by size:
4744 2964 1179 939 552 480 255
NdeI CA'TA_TG
Cuts at: 387 4069 387
Size: 3682 7431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11113
NotI GC'GGCC_GC
Cuts at: 8560 8560
Size: 11113
NsiI A_TGCA'T
Cuts at: 2800 7203 2800
Size: 4403 6710
NspI r_CATG'y
Cuts at: 1132 1229 3344 3556 4151 4228 4705 5371 5506
Size: 97 2115 212 595 77 477 666 135
Cuts at: 5506 7507 7711 8471 10016 1132
Size: 2001 204 760 1545 2229
Fragments arranged by size:
2229 2115 2001 1545 760 666 595 477
212 204 135 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11113
PciI A'CATG_T
Cuts at: 4224 10012 4224
Size: 5788 5325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 3271 6514 6934 8300 1108
Size: 1467 696 3243 420 1366 3921
Fragments arranged by size:
3921 3243 1467 1366 696 420
PfoI T'CCnGG_A
Cuts at: 3134 4184 4218 6195 3134
Size: 1050 34 1977 8052
Fragments arranged by size:
8052 1977 1050 34
PmeI GTTT'AAAC
Cuts at: 8417 8417
Size: 11113
PmlI CAC'GTG
Cuts at: 2390 2390
Size: 11113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9182 9214 10695 10727 9182
Size: 32 1481 32 9568
Fragments arranged by size:
9568 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3132 3241 3878 7627 3132
Size: 109 637 3749 6618
Fragments arranged by size:
6618 3749 637 109
PshAI GACnn'nnGTC
Cuts at: 1613 3876 1613
Size: 2263 8850
PsiI TTA'TAA
Cuts at: 8683 8683
Size: 11113
PspOMI G'GGCC_C
Cuts at: 6051 6051
Size: 11113
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6176 6208 7295 7327 6176
Size: 32 1087 32 9962
Fragments arranged by size:
9962 1087 32 32
PstI C_TGCA'G
Cuts at: 830 2811 4557 5118 5577 5919 6114 6524 7326
Size: 1981 1746 561 459 342 195 410 802
Cuts at: 7326 7692 8465 830
Size: 366 773 3478
Fragments arranged by size:
3478 1981 1746 802 773 561 459 410
366 342 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11113
PvuII CAG'CTG
Cuts at: 2411 2920 3727 3826 4883 5014 5212 5848 7714
Size: 509 807 99 1057 131 198 636 1866
Cuts at: 7714 7891 7999 8125 2411
Size: 177 108 126 5399
Fragments arranged by size:
5399 1866 1057 807 636 509 198 177
131 126 108 99
SacI G_AGCT'C
Cuts at: 6119 7497 6119
Size: 1378 9735
SalI G'TCGA_C
Cuts at: 1936 8455 1936
Size: 6519 4594
SapI GCTCTTCn'nnn_
Cuts at: 2200 6484 6914 7502 2200
Size: 4284 430 588 5811
Fragments arranged by size:
5811 4284 588 430
SbfI CC_TGCA'GG
Cuts at: 7326 7692 8465 7326
Size: 366 773 9974
Fragments arranged by size:
9974 773 366
ScaI AGT'ACT
Cuts at: 4637 5801 7007 9361 11073 4637
Size: 1164 1206 2354 1712 4677
Fragments arranged by size:
4677 2354 1712 1206 1164
SexAI A'CCwGG_T
Cuts at: 3243 6981 3243
Size: 3738 7375
SfcI C'TryA_G
Cuts at: 826 1045 2807 4553 4631 5114 5573 5915 6110
Size: 219 1762 1746 78 483 459 342 195
Cuts at: 6110 6520 7322 7688 8461 9596 10277 10468 826
Size: 410 802 366 773 1135 681 191 1471
Fragments arranged by size:
1762 1746 1471 1135 802 773 681 483
459 410 366 342 219 195 191 78
SfoI GGC'GCC
Cuts at: 7437 7437
Size: 11113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11113
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11113
SmaI CCC'GGG
Cuts at: 1872 8440 1872
Size: 6568 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3482 4832 5726 6830 9217 10118
Size: 197 926 1539 1350 894 1104 2387 901
Cuts at: 10118 10380 820
Size: 262 1553
Fragments arranged by size:
2387 1553 1539 1350 1104 926 901 894
262 197
SnaBI TAC'GTA
Cuts at: 493 8904 493
Size: 8411 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11113
SphI G_CATG'C
Cuts at: 1132 3556 5506 7711 8471 1132
Size: 2424 1950 2205 760 3774
Fragments arranged by size:
3774 2424 2205 1950 760
SspI AAT'ATT
Cuts at: 5 52 9037 5
Size: 47 8985 2081
Fragments arranged by size:
8985 2081 47
StuI AGG'CCT
Cuts at: 1517 7778 1517
Size: 6261 4852
StyI C'CwwG_G
Cuts at: 513 1065 2004 3183 3256 3642 4014 4593 4743
Size: 552 939 1179 73 386 372 579 150
Cuts at: 4743 6147 6495 6570 6627 6882 7447 7524 7869
Size: 1404 348 75 57 255 565 77 345
Cuts at: 7869 8268 8552 513
Size: 399 284 3074
Fragments arranged by size:
3074 1404 1179 939 579 565 552 399
386 372 348 345 284 255 150 77
75 73 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 4398 4511 7794 7868 8188 9140
Size: 1255 2256 562 113 3283 74 320 952
Cuts at: 9140 9157 9310 9495 9940 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3283 2256 1498 1255 952 562 445 320
185 153 113 74 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2040 4503 4635
Size: 274 80 33 51 1443 63 2463 132
Cuts at: 4635 5799 6135 7005 8322 8361 9359 11071 96
Size: 1164 336 870 1317 39 998 1712 138
Fragments arranged by size:
2463 1712 1443 1317 1164 998 870 336
274 138 132 80 63 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 11073 11105 2379
Size: 32 8662 32 2387
Fragments arranged by size:
8662 2387 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11113
XbaI T'CTAG_A
Cuts at: 8449 8449
Size: 11113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 3640 5599 5628 6154 6463 7010 8275 2372
Size: 1268 1959 29 526 309 547 1265 5210
Fragments arranged by size:
5210 1959 1268 1265 547 526 309 29
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11113
XmaI C'CCGG_G
Cuts at: 1870 8438 1870
Size: 6568 4545
XmnI GAAnn'nnTTC
Cuts at: 5444 7139 9242 5444
Size: 1695 2103 7315
Fragments arranged by size:
7315 2103 1695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8921 276
Size: 53 83 186 936 7387 2468
Fragments arranged by size:
7387 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BsiWI FseI FspAI
NaeI NgoMIV NruI PacI PspXI RsrII SacII SanDI
SfiI SrfI SwaI