Restriction map for
pFN21AE2311
(Circular) (Six-base) MAPSORT of: pFN21AE2311.seq Check: 6911 from: 1 to: 10168
pFN21AE2311 10168 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7978 278
Size: 53 83 186 936 6442 2468
Fragments arranged by size:
6442 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7511 1937
Size: 5574 4594
Acc65I G'GTAC_C
Cuts at: 5405 5474 7489 5405
Size: 69 2015 8084
Fragments arranged by size:
8084 2015 69
AclI AA'CG_TT
Cuts at: 1563 8295 8668 1563
Size: 6732 373 3063
Fragments arranged by size:
6732 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2777 4443 5203 5415 6561 7374 8231 9615
Size: 1145 1666 760 212 1146 813 857 1384
Cuts at: 9615 9883 1632
Size: 268 1917
Fragments arranged by size:
1917 1666 1384 1146 1145 857 813 760
268 212
AflII C'TTAA_G
Cuts at: 820 1017 2665 820
Size: 197 1648 8323
Fragments arranged by size:
8323 1648 197
AflIII A'CryG_T
Cuts at: 1203 9067 10044 1203
Size: 7864 977 1327
Fragments arranged by size:
7864 1327 977
AgeI A'CCGG_T
Cuts at: 8999 8999
Size: 10168
AhdI GACnn_n'nnGTC
Cuts at: 8897 8897
Size: 10168
AleI CACnn'nnGTG
Cuts at: 1166 2836 5600 1166
Size: 1670 2764 5734
Fragments arranged by size:
5734 2764 1670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6242 1184
Size: 5058 5110
AlwNI CAG_nnn'CTG
Cuts at: 2107 2191 7911 9483 2107
Size: 84 5720 1572 2792
Fragments arranged by size:
5720 2792 1572 84
ApaLI G'TGCA_C
Cuts at: 2775 8225 9381 2775
Size: 5450 1156 3562
Fragments arranged by size:
5450 3562 1156
ApoI r'AATT_y
Cuts at: 1493 2271 2538 2942 3358 5753 6643 7477 7703
Size: 778 267 404 416 2395 890 834 226
Cuts at: 7703 7855 9964 1493
Size: 152 2109 1697
Fragments arranged by size:
2395 2109 1697 890 834 778 416 404
267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4587 8722 160
Size: 872 3555 4135 1606
Fragments arranged by size:
4135 3555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10168
AvaI C'yCGr_G
Cuts at: 1870 1943 4181 7493 1870
Size: 73 2238 3312 4545
Fragments arranged by size:
4545 3312 2238 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5270 5303 5397 5430 5270
Size: 33 94 33 10008
Fragments arranged by size:
10008 94 33 33
BamHI G'GATC_C
Cuts at: 7498 7865 7498
Size: 367 9801
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5405 5474 7489 8944 618
Size: 325 217 627 3618 69 2015 1455 1842
Fragments arranged by size:
3618 2015 1842 1455 627 325 217 69
BanII G_rGCy'C
Cuts at: 1391 2049 5944 7487 1391
Size: 658 3895 1543 4072
Fragments arranged by size:
4072 3895 1543 658
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2404 2756 7172 7389 928
Size: 971 424 81 352 4416 217 3707
Fragments arranged by size:
4416 3707 971 424 352 217 81
BbvCI CC'TCA_GC
Cuts at: 5548 5548
Size: 10168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4460 4494 8359 8393 1903
Size: 34 2523 34 3865 34 3678
Fragments arranged by size:
3865 3678 2523 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2440 3125 8060 9270 2440
Size: 685 4935 1210 3338
Fragments arranged by size:
4935 3338 1210 685
BclI T'GATC_A
Cuts at: 1548 2221 1548
Size: 673 9495
BfrBI ATG'CAT
Cuts at: 2530 6611 2530
Size: 4081 6087
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8779 136
Size: 107 122 71 631 605 7107 1525
Fragments arranged by size:
7107 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4400 6040 7186 10163 4400
Size: 1640 1146 2977 4405
Fragments arranged by size:
4405 2977 1640 1146
BlpI GC'TnA_GC
Cuts at: 7585 7585
Size: 10168
Bme1580I G_kGCm'C
Cuts at: 1790 2779 8229 9385 1790
Size: 989 5450 1156 2573
Fragments arranged by size:
5450 2573 1156 989
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2880 5938 7899
Size: 416 53 83 399 24 1463 3058 1961
Cuts at: 7899 8857 442
Size: 958 1753
Fragments arranged by size:
3058 1961 1753 1463 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7366 7366
Size: 10168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2310 2985 4534 8828 1239
Size: 1071 675 1549 4294 2579
Fragments arranged by size:
4294 2579 1549 1071 675
Bpu10I CC'TnA_GC
Cuts at: 2100 5548 2100
Size: 3448 6720
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 5185 5303 8293 9158 9456 2507
Size: 2678 118 2990 865 298 3219
Fragments arranged by size:
3219 2990 2678 865 298 118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8831 882
Size: 617 7332 2219
Fragments arranged by size:
7332 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3173 5933 7959 493
Size: 711 1969 2760 2026 2702
Fragments arranged by size:
2760 2702 2026 1969 711
BsaBI GATnn'nnATC
Cuts at: 2860 7185 10162 2860
Size: 4325 2977 2866
Fragments arranged by size:
4325 2977 2866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6794 7975 8357 8992
Size: 53 83 186 936 5261 1181 382 635
Cuts at: 8992 275
Size: 1451
Fragments arranged by size:
5261 1451 1181 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8601 8999 9273 9420 9831 8601
Size: 398 274 147 411 8938
Fragments arranged by size:
8938 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 9943
Fragments arranged by size:
9943 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3513 3860 7373 1186
Size: 2327 347 3513 3981
Fragments arranged by size:
3981 3513 2327 347
BseYI C'CCAG_C
Cuts at: 2435 5322 6119 6405 9371 9867 2435
Size: 2887 797 286 2966 496 2736
Fragments arranged by size:
2966 2887 2736 797 496 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2860 5455 1163
Size: 573 1124 2595 5876
Fragments arranged by size:
5876 2595 1124 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2042 7618 8379 9407 1540
Size: 275 186 41 5576 761 1028 2301
Fragments arranged by size:
5576 2301 1028 761 275 186 41
BsiHKAI G_wGCw'C
Cuts at: 2049 2779 8229 8314 9385 2049
Size: 730 5450 85 1071 2832
Fragments arranged by size:
5450 2832 1071 730 85
BsmI GAATG_Cn'
Cuts at: 6434 7679 7772 6434
Size: 1245 93 8830
Fragments arranged by size:
8830 1245 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3967 3967
Size: 10168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2779 5944 7487 8229 8314 9385
Size: 399 259 730 3165 1543 742 85 1071
Cuts at: 9385 1391
Size: 2174
Fragments arranged by size:
3165 2174 1543 1071 742 730 399 259
85
BspEI T'CCGG_A
Cuts at: 9831 9831
Size: 10168
BspHI T'CATG_A
Cuts at: 3397 3463 4333 8057 9787 3397
Size: 66 870 3724 1730 3778
Fragments arranged by size:
3778 3724 1730 870 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2944 3980 7523 844
Size: 2100 1036 3543 3489
Fragments arranged by size:
3543 3489 2100 1036
BsrBI CCG'CTC
Cuts at: 1394 1823 4144 8055 1394
Size: 429 2321 3911 3507
Fragments arranged by size:
3911 3507 2321 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 8663 8837 65
Size: 1183 1191 6224 174 1396
Fragments arranged by size:
6224 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6034 8812 8999 1700
Size: 110 4224 2778 187 2869
Fragments arranged by size:
4224 2869 2778 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10168
BssSI C'ACGA_G
Cuts at: 8228 9240 8228
Size: 1012 9156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10168
BstBI TT'CG_AA
Cuts at: 2767 6517 7380 8972 2767
Size: 3750 863 1592 3963
Fragments arranged by size:
3963 3750 1592 863
BstEII G'GTnAC_C
Cuts at: 1184 3788 5210 1184
Size: 2604 1422 6142
Fragments arranged by size:
6142 2604 1422
BstXI CCAn_nnnn'nTGG
Cuts at: 3545 4836 3545
Size: 1291 8877
BstYI r'GATC_y
Cuts at: 1972 2369 3487 4147 4348 4400 5083 5569 5617
Size: 397 1118 660 201 52 683 486 48
Cuts at: 5617 6040 6393 6544 7186 7498 7865 8250 8267
Size: 423 353 151 642 312 367 385 17
Cuts at: 8267 9719 9805 9817 10163 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1118 683 660 642 486 423
397 385 367 353 346 312 201 151
86 52 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3880 3880
Size: 10168
BtgI C'CryG_G
Cuts at: 513 1065 4979 5565 6883 513
Size: 552 3914 586 1318 3798
Fragments arranged by size:
3914 3798 1318 586 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 5212 487
Size: 683 56 818 3168 5443
Fragments arranged by size:
5443 3168 818 683 56
BtsI GCAGTG_nn'
Cuts at: 2514 2848 7684 8478 8498 2514
Size: 334 4836 794 20 4184
Fragments arranged by size:
4836 4184 794 334 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10133
DraI TTT'AAA
Cuts at: 3187 7472 3187
Size: 4285 5883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4258 9175 809
Size: 507 2942 4917 1802
Fragments arranged by size:
4917 2942 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 3692 3826
Size: 54 1425 50 275 18 291 1571 134
Cuts at: 3826 6880 7615 8504 10133 8
Size: 3054 735 889 1629 43
Fragments arranged by size:
3054 1629 1571 1425 889 735 291 275
134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7615 1537
Size: 275 5803 4090
Fragments arranged by size:
5803 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 3187 3197 4260 4407 4428 4503 5280
Size: 909 925 10 1063 147 21 75 777
Cuts at: 5280 5856 6294 6905 7260 7350 8098 1353
Size: 576 438 611 355 90 748 3423
Fragments arranged by size:
3423 1063 925 909 777 748 611 576
438 355 147 90 75 21 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5124 8755 9129 9275 387
Size: 1064 138 3535 3631 374 146 1280
Fragments arranged by size:
3631 3535 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2047 2047
Size: 10168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2310 2777 2985 4443 4534 5203 5415
Size: 393 678 467 208 1458 91 669 212
Cuts at: 5415 6561 7374 8231 8828 9615 9883 1239
Size: 1146 813 857 597 787 268 1524
Fragments arranged by size:
1524 1458 1146 857 813 787 678 669
597 467 393 268 212 208 91
EcoO109I rG'GnC_Cy
Cuts at: 2178 2252 6917 2178
Size: 74 4665 5429
Fragments arranged by size:
5429 4665 74
EcoRI G'AATT_C
Cuts at: 2538 3358 6643 7477 7855 2538
Size: 820 3285 834 378 4851
Fragments arranged by size:
4851 3285 834 820 378
EcoRV GAT'ATC
Cuts at: 2858 3809 2858
Size: 951 9217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2340 7347 2340
Size: 5007 5161
FspI TGC'GCA
Cuts at: 8674 8674
Size: 10168
HaeII r_GCGC'y
Cuts at: 1678 2956 9315 1678
Size: 1278 6359 2531
Fragments arranged by size:
6359 2531 1278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2031 2056 2063 3062
Size: 32 135 32 452 7 25 7 999
Cuts at: 3062 3094 3649 3681 5423 5430 5455 5462 5531
Size: 32 555 32 1742 7 25 7 69
Cuts at: 5531 5563 6266 6298 6551 6583 7231 7263 7407
Size: 32 703 32 253 32 648 32 144
Cuts at: 7407 7439 8809 8841 8883 8915 1373
Size: 32 1370 32 42 32 2626
Fragments arranged by size:
2626 1742 1370 999 703 648 555 452
253 144 135 69 42 32 32 32
32 32 32 32 32 32 32 32
25 25 7 7 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 3109 3374 7434 7512 7758
Size: 988 281 481 690 265 4060 78 246
Cuts at: 7758 669
Size: 3079
Fragments arranged by size:
4060 3079 988 690 481 281 265 246
78
HindIII A'AGCT_T
Cuts at: 748 6663 748
Size: 5915 4253
HpaI GTT'AAC
Cuts at: 2419 7758 2419
Size: 5339 4829
KpnI G_GTAC'C
Cuts at: 5409 5478 7493 5409
Size: 69 2015 8084
Fragments arranged by size:
8084 2015 69
MfeI C'AATT_G
Cuts at: 7767 7767
Size: 10168
MluI A'CGCG_T
Cuts at: 10044 10044
Size: 10168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2525 3150 3195 4930 5636 9282 9466 1305
Size: 1220 625 45 1735 706 3646 184 2007
Fragments arranged by size:
3646 2007 1735 1220 706 625 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6882 10
Size: 54 1425 343 5050 3296
Fragments arranged by size:
5050 3296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2836 3275 3402 4332 4527 4834
Size: 648 1359 311 439 127 930 195 307
Cuts at: 4834 5600 6083 8126 8485 8644 518
Size: 766 483 2043 359 159 2042
Fragments arranged by size:
2043 2042 1359 930 766 648 483 439
359 311 307 195 159 127
MspA1I CmG'CkG
Cuts at: 1606 2729 4193 6581 6818 7372 7584 8261 9409
Size: 1123 1464 2388 237 554 212 677 1148
Cuts at: 9409 9654 1606
Size: 245 2120
Fragments arranged by size:
2388 2120 1464 1148 1123 677 554 245
237 212
NcoI C'CATG_G
Cuts at: 513 1065 4979 6883 513
Size: 552 3914 1904 3798
Fragments arranged by size:
3914 3798 1904 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10168
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10168
NotI GC'GGCC_GC
Cuts at: 7615 7615
Size: 10168
NsiI A_TGCA'T
Cuts at: 2532 6613 2532
Size: 4081 6087
NspI r_CATG'y
Cuts at: 1132 1229 2357 2678 3597 7080 7526 9071 1132
Size: 97 1128 321 919 3483 446 1545 2229
Fragments arranged by size:
3483 2229 1545 1128 919 446 321 97
PasI CC'CwG_GG
Cuts at: 1348 5484 1348
Size: 4136 6032
PciI A'CATG_T
Cuts at: 9067 9067
Size: 10168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 1108
Size: 1083 9085
PfoI T'CCnGG_A
Cuts at: 2070 2287 4344 2070
Size: 217 2057 7894
Fragments arranged by size:
7894 2057 217
PmeI GTTT'AAAC
Cuts at: 7472 7472
Size: 10168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5878 5910 8237 8269 9750 9782 5878
Size: 32 2327 32 1481 32 6264
Fragments arranged by size:
6264 2327 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2178 2252 6917 2178
Size: 74 4665 5429
Fragments arranged by size:
5429 4665 74
PshAI GACnn'nnGTC
Cuts at: 1613 2551 1613
Size: 938 9230
PsiI TTA'TAA
Cuts at: 3323 5783 5802 5820 6279 7738 3323
Size: 2460 19 18 459 1459 5753
Fragments arranged by size:
5753 2460 1459 459 19 18
PstI C_TGCA'G
Cuts at: 830 2509 3331 4596 7520 830
Size: 1679 822 1265 2924 3478
Fragments arranged by size:
3478 2924 1679 1265 822
PvuI CG_AT'CG
Cuts at: 2001 2042 2001
Size: 41 10127
PvuII CAG'CTG
Cuts at: 2729 4193 6581 6818 7372 2729
Size: 1464 2388 237 554 5525
Fragments arranged by size:
5525 2388 1464 554 237
SacI G_AGCT'C
Cuts at: 2049 2049
Size: 10168
SalI G'TCGA_C
Cuts at: 1936 7510 1936
Size: 5574 4594
SapI GCTCTTCn'nnn_
Cuts at: 3197 6294 3197
Size: 3097 7071
SbfI CC_TGCA'GG
Cuts at: 7520 7520
Size: 10168
ScaI AGT'ACT
Cuts at: 7062 8416 10128 7062
Size: 1354 1712 7102
Fragments arranged by size:
7102 1712 1354
SfcI C'TryA_G
Cuts at: 826 1045 2505 2822 3327 4592 6061 7001 7251
Size: 219 1460 317 505 1265 1469 940 250
Cuts at: 7251 7516 8651 9332 9523 826
Size: 265 1135 681 191 1471
Fragments arranged by size:
1471 1469 1460 1265 1135 940 681 505
317 265 250 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10168
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10168
SmaI CCC'GGG
Cuts at: 1872 7495 1872
Size: 5623 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 2665 5164 5282 8272 9173
Size: 197 926 543 179 2499 118 2990 901
Cuts at: 9173 9435 820
Size: 262 1553
Fragments arranged by size:
2990 2499 1553 926 901 543 262 197
179 118
SnaBI TAC'GTA
Cuts at: 493 5933 7959 493
Size: 5440 2026 2702
Fragments arranged by size:
5440 2702 2026
SpeI A'CTAG_T
Cuts at: 152 3131 152
Size: 2979 7189
SphI G_CATG'C
Cuts at: 1132 3597 7526 1132
Size: 2465 3929 3774
Fragments arranged by size:
3929 3774 2465
SspI AAT'ATT
Cuts at: 5 52 8092 5
Size: 47 8040 2081
Fragments arranged by size:
8040 2081 47
StuI AGG'CCT
Cuts at: 1517 2025 1517
Size: 508 9660
StyI C'CwwG_G
Cuts at: 513 1065 3538 4979 5354 6883 7607 513
Size: 552 2473 1441 375 1529 724 3074
Fragments arranged by size:
3074 2473 1529 1441 724 552 375
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 4874 8195 8212 8365 8550 8995
Size: 1255 869 2425 3321 17 153 185 445
Cuts at: 8995 325
Size: 1498
Fragments arranged by size:
3321 2425 1498 1255 869 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2293 2562 5624
Size: 274 80 33 51 1443 316 269 3062
Cuts at: 5624 6781 7060 8414 10126 96
Size: 1157 279 1354 1712 138
Fragments arranged by size:
3062 1712 1443 1354 1157 316 279 274
269 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5194 5226 10128 10160 5194
Size: 32 4902 32 5202
Fragments arranged by size:
5202 4902 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2085 1316
Size: 769 9399
XbaI T'CTAG_A
Cuts at: 5080 7504 5080
Size: 2424 7744
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10168
XmaI C'CCGG_G
Cuts at: 1870 7493 1870
Size: 5623 4545
XmnI GAAnn'nnTTC
Cuts at: 5274 7062 8297 5274
Size: 1788 1235 7145
Fragments arranged by size:
7145 1788 1235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7976 276
Size: 53 83 186 936 6442 2468
Fragments arranged by size:
6442 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI
BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI