Restriction map for
pFN21AE2309
(Circular) (Six-base) MAPSORT of: pFN21AE2309.seq Check: 6423 from: 1 to: 8476
pFN21AE2309 8476 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3258 3653 4273 3258
Size: 395 620 7461
Fragments arranged by size:
7461 620 395
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6286 278
Size: 53 83 186 936 4750 2468
Fragments arranged by size:
4750 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5148 5819 1937
Size: 3211 671 4594
Fragments arranged by size:
4594 3211 671
Acc65I G'GTAC_C
Cuts at: 2493 5797 2493
Size: 3304 5172
AclI AA'CG_TT
Cuts at: 1563 6603 6976 1563
Size: 5040 373 3063
Fragments arranged by size:
5040 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3399 3854 4563 4617 6539 7923 8191 1632
Size: 1767 455 709 54 1922 1384 268 1917
Fragments arranged by size:
1922 1917 1767 1384 709 455 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8279
AflIII A'CryG_T
Cuts at: 1203 4341 7375 8352 1203
Size: 3138 3034 977 1327
Fragments arranged by size:
3138 3034 1327 977
AgeI A'CCGG_T
Cuts at: 7307 7307
Size: 8476
AhdI GACnn_n'nnGTC
Cuts at: 2203 7205 2203
Size: 5002 3474
AleI CACnn'nnGTG
Cuts at: 1166 2816 5717 1166
Size: 1650 2901 3925
Fragments arranged by size:
3925 2901 1650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8476
AlwNI CAG_nnn'CTG
Cuts at: 2842 5462 6219 7791 2842
Size: 2620 757 1572 3527
Fragments arranged by size:
3527 2620 1572 757
ApaLI G'TGCA_C
Cuts at: 6533 7689 6533
Size: 1156 7320
ApoI r'AATT_y
Cuts at: 1493 5785 6011 6163 8272 1493
Size: 4292 226 152 2109 1697
Fragments arranged by size:
4292 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7030 160
Size: 872 5998 1606
Fragments arranged by size:
5998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8476
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2303 2600 3101 3553 3591 4053
Size: 73 170 190 297 501 452 38 462
Cuts at: 4053 5327 5434 5801 1870
Size: 1274 107 367 4545
Fragments arranged by size:
4545 1274 501 462 452 367 297 190
170 107 73 38
BamHI G'GATC_C
Cuts at: 3587 3800 5806 6173 3587
Size: 213 2006 367 5890
Fragments arranged by size:
5890 2006 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2408 2493 3047 3081 3094
Size: 325 217 627 621 85 554 34 13
Cuts at: 3094 3336 3656 5668 5797 7252 618
Size: 242 320 2012 129 1455 1842
Fragments arranged by size:
2012 1842 1455 627 621 554 325 320
242 217 129 85 34 13
BanII G_rGCy'C
Cuts at: 1391 2925 3019 3367 3488 3506 3524 3608 3623
Size: 1534 94 348 121 18 18 84 15
Cuts at: 3623 4259 4424 4457 4647 5539 5572 5795 1391
Size: 636 165 33 190 892 33 223 4072
Fragments arranged by size:
4072 1534 892 636 348 223 190 165
121 94 84 33 33 18 18 15
BbeI G_GCGC'C
Cuts at: 2412 3051 2412
Size: 639 7837
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 3180 3247 3366 5257 928
Size: 971 151 1130 67 119 1891 4147
Fragments arranged by size:
4147 1891 1130 971 151 119 67
BbvCI CC'TCA_GC
Cuts at: 5350 5393 5350
Size: 43 8433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3025 3059 6667 6701 1903
Size: 34 1088 34 3608 34 3678
Fragments arranged by size:
3678 3608 1088 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6368 7578 6368
Size: 1210 7266
BclI T'GATC_A
Cuts at: 1548 2445 1548
Size: 897 7579
BfrBI ATG'CAT
Cuts at: 2965 2965
Size: 8476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2381 2622 2663
Size: 107 122 71 631 605 709 241 41
Cuts at: 2663 3259 4114 7087 136
Size: 596 855 2973 1525
Fragments arranged by size:
2973 1525 855 709 631 605 596 241
122 107 71 41
BglII A'GATC_T
Cuts at: 4933 8471 4933
Size: 3538 4938
BlpI GC'TnA_GC
Cuts at: 2585 2753 3310 5893 2585
Size: 168 557 2583 5168
Fragments arranged by size:
5168 2583 557 168
Bme1580I G_kGCm'C
Cuts at: 1790 2280 2742 3659 3672 6537 7693 1790
Size: 490 462 917 13 2865 1156 2573
Fragments arranged by size:
2865 2573 1156 917 490 462 13
BmgBI CAC'GTC
Cuts at: 1318 3329 5741 1318
Size: 2011 2412 4053
Fragments arranged by size:
4053 2412 2011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4558 6207 7165
Size: 416 53 83 399 24 3141 1649 958
Cuts at: 7165 442
Size: 1753
Fragments arranged by size:
3141 1753 1649 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 4327 3134
Size: 1193 7283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3214 3523 3717 3753 3945 4129 4377 4626
Size: 1975 309 194 36 192 184 248 249
Cuts at: 4626 5586 7136 1239
Size: 960 1550 2579
Fragments arranged by size:
2579 1975 1550 960 309 249 248 194
192 184 36
Bpu10I CC'TnA_GC
Cuts at: 2843 3487 3505 3622 3712 3748 3784 5350 5393
Size: 644 18 117 90 36 36 1566 43
Cuts at: 5393 2843
Size: 5926
Fragments arranged by size:
5926 1566 644 117 90 43 36 36
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4803 6601 7466 7764 4803
Size: 1798 865 298 5515
Fragments arranged by size:
5515 1798 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3685 4660 5328 7139 882
Size: 617 2186 975 668 1811 2219
Fragments arranged by size:
2219 2186 1811 975 668 617
BsaAI yAC'GTr
Cuts at: 493 1204 4105 4955 5719 6267 493
Size: 711 2901 850 764 548 2702
Fragments arranged by size:
2901 2702 850 764 711 548
BsaBI GATnn'nnATC
Cuts at: 8470 8470
Size: 8476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2409 3048 5254
Size: 53 83 186 936 783 93 639 2206
Cuts at: 5254 6283 6665 7300 275
Size: 1029 382 635 1451
Fragments arranged by size:
2206 1451 1029 936 783 639 635 382
186 93 83 53
BsaWI w'CCGG_w
Cuts at: 5641 6909 7307 7581 7728 8139 5641
Size: 1268 398 274 147 411 5978
Fragments arranged by size:
5978 1268 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2780 2810 4792 4822 1178
Size: 30 165 30 1377 30 1982 30 4832
Fragments arranged by size:
4832 1982 1377 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2801 3894 3897 4002 4158 1186
Size: 1042 573 1093 3 105 156 5504
Fragments arranged by size:
5504 1093 1042 573 156 105 3
BseYI C'CCAG_C
Cuts at: 2320 2734 3209 3317 3322 5189 7679 8175 2320
Size: 414 475 108 5 1867 2490 496 2621
Fragments arranged by size:
2621 2490 1867 496 475 414 108 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2076 3021 3230 4068 1163
Size: 573 340 945 209 838 5571
Fragments arranged by size:
5571 945 838 573 340 209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2127 2400 2658 5926 6687 7715
Size: 275 186 126 273 258 3268 761 1028
Cuts at: 7715 1540
Size: 2301
Fragments arranged by size:
3268 2301 1028 761 275 273 258 186
126
BsiHKAI G_wGCw'C
Cuts at: 2298 2925 3367 3641 4424 4457 6537 6622 7693
Size: 627 442 274 783 33 2080 85 1071
Cuts at: 7693 2298
Size: 3081
Fragments arranged by size:
3081 2080 1071 783 627 442 274 85
33
BsmI GAATG_Cn'
Cuts at: 4007 5987 6080 4007
Size: 1980 93 6403
Fragments arranged by size:
6403 1980 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2280 2298 2742 2925 3019 3367 3488
Size: 399 490 18 444 183 94 348 121
Cuts at: 3488 3506 3524 3608 3623 3641 3659 3672 4259
Size: 18 18 84 15 18 18 13 587
Cuts at: 4259 4424 4457 4647 5539 5572 5795 6537 6622
Size: 165 33 190 892 33 223 742 85
Cuts at: 6622 7693 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 892 742 587 490 444 399
348 223 190 183 165 121 94 85
84 33 33 18 18 18 18 18
15 13
BspEI T'CCGG_A
Cuts at: 5641 8139 5641
Size: 2498 5978
BspHI T'CATG_A
Cuts at: 4639 6365 8095 4639
Size: 1726 1730 5020
Fragments arranged by size:
5020 1730 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2265 2701 2886 2923 3255 3258 3653
Size: 1385 36 436 185 37 332 3 395
Cuts at: 3653 4273 5831 844
Size: 620 1558 3489
Fragments arranged by size:
3489 1558 1385 620 436 395 332 185
37 36 3
BsrBI CCG'CTC
Cuts at: 1394 1823 2425 2757 3100 3278 5639 6363 1394
Size: 429 602 332 343 178 2361 724 3507
Fragments arranged by size:
3507 2361 724 602 429 343 332 178
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 5032 5082 6971 7145 65
Size: 1183 1257 2527 50 1889 174 1396
Fragments arranged by size:
2527 1889 1396 1257 1183 174 50
BsrFI r'CCGG_y
Cuts at: 1700 1810 2653 3289 4361 5549 7120 7307 1700
Size: 110 843 636 1072 1188 1571 187 2869
Fragments arranged by size:
2869 1571 1188 1072 843 636 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8476
BssHII G'CGCG_C
Cuts at: 1925 2634 1925
Size: 709 7767
BssSI C'ACGA_G
Cuts at: 6536 7548 6536
Size: 1012 7464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5591 794
Size: 4797 3679
BstBI TT'CG_AA
Cuts at: 7280 7280
Size: 8476
BstEII G'GTnAC_C
Cuts at: 1184 2092 1184
Size: 908 7568
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 3695 3731 4836 2734
Size: 961 36 1105 6374
Fragments arranged by size:
6374 1105 961 36
BstYI r'GATC_y
Cuts at: 1972 3587 3800 3842 4035 4933 5806 6173 6558
Size: 1615 213 42 193 898 873 367 385
Cuts at: 6558 6575 8027 8113 8125 8471 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1615 1452 898 873 385 367 346
213 193 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 3152 4392 3152
Size: 1240 7236
BtgI C'CryG_G
Cuts at: 513 1065 2004 2175 2376 2435 3742 5088 513
Size: 552 939 171 201 59 1307 1346 3901
Fragments arranged by size:
3901 1346 1307 939 552 201 171 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2552 487
Size: 683 56 1326 6411
Fragments arranged by size:
6411 1326 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 5992 6786 6806 2094
Size: 3898 794 20 3764
Fragments arranged by size:
3898 3764 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4117 4152 529
Size: 35 3553 35 4853
Fragments arranged by size:
4853 3553 35 35
DraI TTT'AAA
Cuts at: 5780 5780
Size: 8476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2146 2225 2789 4535 7483 809
Size: 507 830 79 564 1746 2948 1802
Fragments arranged by size:
2948 1802 1746 830 564 507 79
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2397 2544
Size: 54 1425 50 275 18 348 219 147
Cuts at: 2544 2655 2820 3291 3496 3514 3529 3613 3649
Size: 111 165 471 205 18 15 84 36
Cuts at: 3649 3685 3721 3739 4125 4988 5923 6812 8441
Size: 36 36 18 386 863 935 889 1629
Cuts at: 8441 8
Size: 43
Fragments arranged by size:
1629 1425 935 889 863 471 386 348
275 219 205 165 147 111 84 54
50 43 36 36 36 18 18 18
15
EagI C'GGCC_G
Cuts at: 1537 1812 2397 2655 5923 1537
Size: 275 585 258 3268 4090
Fragments arranged by size:
4090 3268 585 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 4408 5034 5232 6406 1353
Size: 852 2203 626 198 1174 3423
Fragments arranged by size:
3423 2203 1174 852 626 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 5464 7063 7437 7583 387
Size: 1064 138 1107 2768 1599 374 146 1280
Fragments arranged by size:
2768 1599 1280 1107 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2923 3365 4422 4455 2923
Size: 442 1057 33 6944
Fragments arranged by size:
6944 1057 442 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3214 3399 3523 3717 3753 3854 3945
Size: 393 1582 185 124 194 36 101 91
Cuts at: 3945 4129 4377 4563 4617 4626 5586 6539 7136
Size: 184 248 186 54 9 960 953 597
Cuts at: 7136 7923 8191 1239
Size: 787 268 1524
Fragments arranged by size:
1582 1524 960 953 787 597 393 268
248 194 186 185 184 124 101 91
54 36 9
EcoO109I rG'GnC_Cy
Cuts at: 2218 3126 3728 4058 4522 4552 2218
Size: 908 602 330 464 30 6142
Fragments arranged by size:
6142 908 602 464 330 30
EcoRI G'AATT_C
Cuts at: 5785 6163 5785
Size: 378 8098
FspI TGC'GCA
Cuts at: 6982 6982
Size: 8476
HaeII r_GCGC'y
Cuts at: 1678 2287 2412 2614 3051 3199 4198 7623 1678
Size: 609 125 202 437 148 999 3425 2531
Fragments arranged by size:
3425 2531 999 609 437 202 148 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2540 2572 2836
Size: 32 135 32 617 32 319 32 264
Cuts at: 2836 2868 4601 4633 4689 4721 5286 5318 5462
Size: 32 1733 32 56 32 565 32 144
Cuts at: 5462 5494 7117 7149 7191 7223 1373
Size: 32 1623 32 42 32 2626
Fragments arranged by size:
2626 1733 1623 617 565 319 264 144
135 56 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3982 4771 5149 5820 6066
Size: 988 281 1122 922 789 378 671 246
Cuts at: 6066 669
Size: 3079
Fragments arranged by size:
3079 1122 988 922 789 671 378 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8476
HpaI GTT'AAC
Cuts at: 3060 6066 3060
Size: 3006 5470
KasI G'GCGC_C
Cuts at: 2408 3047 2408
Size: 639 7837
KpnI G_GTAC'C
Cuts at: 2497 5801 2497
Size: 3304 5172
MfeI C'AATT_G
Cuts at: 3478 6075 3478
Size: 2597 5879
MluI A'CGCG_T
Cuts at: 8352 8352
Size: 8476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 4397 5339 7590 7774 1305
Size: 1233 1859 942 2251 184 2007
Fragments arranged by size:
2251 2007 1859 1233 942 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 3516 3531 3615 3651
Size: 54 1425 343 990 694 15 84 36
Cuts at: 3651 3687 3723 3741 4127 4990 10
Size: 36 36 18 386 863 3496
Fragments arranged by size:
3496 1425 990 863 694 386 343 84
54 36 36 36 18 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2816 3470 4238 4346 5717 6434 6793
Size: 648 1650 654 768 108 1371 717 359
Cuts at: 6793 6952 518
Size: 159 2042
Fragments arranged by size:
2042 1650 1371 768 717 654 648 359
159 108
MspA1I CmG'CkG
Cuts at: 1606 2063 2072 2378 2393 2437 2619 2675 3021
Size: 457 9 306 15 44 182 56 346
Cuts at: 3021 3923 5300 5459 5487 5627 5633 5892 6569
Size: 902 1377 159 28 140 6 259 677
Cuts at: 6569 7717 7962 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1377 1148 902 677 457 346 306
259 245 182 159 140 56 44 28
15 9 6
NaeI GCC'GGC
Cuts at: 2655 3291 2655
Size: 636 7840
NarI GG'CG_CC
Cuts at: 2409 3048 2409
Size: 639 7837
NcoI C'CATG_G
Cuts at: 513 1065 2004 3742 5088 513
Size: 552 939 1738 1346 3901
Fragments arranged by size:
3901 1738 1346 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8476
NgoMIV G'CCGG_C
Cuts at: 2653 3289 2653
Size: 636 7840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8476
NotI GC'GGCC_GC
Cuts at: 2397 5923 2397
Size: 3526 4950
NruI TCG'CGA
Cuts at: 2028 2537 2028
Size: 509 7967
NsiI A_TGCA'T
Cuts at: 2967 2967
Size: 8476
NspI r_CATG'y
Cuts at: 1132 1229 2965 3469 4345 5834 7379 1132
Size: 97 1736 504 876 1489 1545 2229
Fragments arranged by size:
2229 1736 1545 1489 876 504 97
PasI CC'CwG_GG
Cuts at: 1348 2727 3427 1348
Size: 1379 700 6397
Fragments arranged by size:
6397 1379 700
PciI A'CATG_T
Cuts at: 4341 7375 4341
Size: 3034 5442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3923 4416 1108
Size: 2815 493 5168
Fragments arranged by size:
5168 2815 493
PfoI T'CCnGG_A
Cuts at: 2155 2914 4354 2155
Size: 759 1440 6277
Fragments arranged by size:
6277 1440 759
PmeI GTTT'AAAC
Cuts at: 5780 5780
Size: 8476
PmlI CAC'GTG
Cuts at: 4955 5719 4955
Size: 764 7712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6545 6577 8058 8090 6545
Size: 32 1481 32 6931
Fragments arranged by size:
6931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 3126 3728 4058 4552 2218
Size: 908 602 330 494 6142
Fragments arranged by size:
6142 908 602 494 330
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8476
PsiI TTA'TAA
Cuts at: 6046 6046
Size: 8476
PspXI vC'TCGA_Gb
Cuts at: 3101 4053 3101
Size: 952 7524
PstI C_TGCA'G
Cuts at: 830 2389 3346 5232 5377 5632 5828 830
Size: 1559 957 1886 145 255 196 3478
Fragments arranged by size:
3478 1886 1559 957 255 196 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8476
PvuII CAG'CTG
Cuts at: 3923 5459 5633 3923
Size: 1536 174 6766
Fragments arranged by size:
6766 1536 174
RsrII CG'GwC_CG
Cuts at: 2912 2912
Size: 8476
SacI G_AGCT'C
Cuts at: 2925 3367 4424 4457 2925
Size: 442 1057 33 6944
Fragments arranged by size:
6944 1057 442 33
SacII CC_GC'GG
Cuts at: 2379 2438 2379
Size: 59 8417
SalI G'TCGA_C
Cuts at: 1936 5147 5818 1936
Size: 3211 671 4594
Fragments arranged by size:
4594 3211 671
SanDI GG'GwC_CC
Cuts at: 4552 4552
Size: 8476
SapI GCTCTTCn'nnn_
Cuts at: 4408 5232 4408
Size: 824 7652
SbfI CC_TGCA'GG
Cuts at: 5828 5828
Size: 8476
ScaI AGT'ACT
Cuts at: 6724 8436 6724
Size: 1712 6764
SfcI C'TryA_G
Cuts at: 826 1045 2385 3031 3342 5016 5214 5228 5373
Size: 219 1340 646 311 1674 198 14 145
Cuts at: 5373 5628 5824 6959 7640 7831 826
Size: 255 196 1135 681 191 1471
Fragments arranged by size:
1674 1471 1340 1135 681 646 311 255
219 198 196 191 145 14
SfoI GGC'GCC
Cuts at: 2410 3049 2410
Size: 639 7837
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8476
SmaI CCC'GGG
Cuts at: 1872 5436 5803 1872
Size: 3564 367 4545
Fragments arranged by size:
4545 3564 367
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 2600 3101 4053 4818 5327
Size: 197 926 360 297 501 952 765 509
Cuts at: 5327 6580 7481 7743 820
Size: 1253 901 262 1553
Fragments arranged by size:
1553 1253 952 926 901 765 509 501
360 297 262 197
SnaBI TAC'GTA
Cuts at: 493 6267 493
Size: 5774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8476
SphI G_CATG'C
Cuts at: 1132 5834 1132
Size: 4702 3774
SspI AAT'ATT
Cuts at: 5 52 6400 5
Size: 47 6348 2081
Fragments arranged by size:
6348 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8476
StyI C'CwwG_G
Cuts at: 513 1065 2004 3532 3742 5088 5915 513
Size: 552 939 1528 210 1346 827 3074
Fragments arranged by size:
3074 1528 1346 939 827 552 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 2900 3223 3331 6503 6520 6673
Size: 1255 954 366 323 108 3172 17 153
Cuts at: 6673 6858 7303 325
Size: 185 445 1498
Fragments arranged by size:
3172 1498 1255 954 445 366 323 185
153 108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6722 8434 96
Size: 274 80 33 51 1443 4745 1712 138
Fragments arranged by size:
4745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 8436 8468 4190
Size: 32 4214 32 4198
Fragments arranged by size:
4214 4198 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2143 4766 1316
Size: 827 2623 5026
Fragments arranged by size:
5026 2623 827
XbaI T'CTAG_A
Cuts at: 3641 5812 3641
Size: 2171 6305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3217 3524 3749 3217
Size: 307 225 7944
Fragments arranged by size:
7944 307 225
XhoI C'TCGA_G
Cuts at: 1943 2303 2600 3101 4053 5327 1943
Size: 360 297 501 952 1274 5092
Fragments arranged by size:
5092 1274 952 501 360 297
XmaI C'CCGG_G
Cuts at: 1870 5434 5801 1870
Size: 3564 367 4545
Fragments arranged by size:
4545 3564 367
XmnI GAAnn'nnTTC
Cuts at: 6605 6605
Size: 8476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6284 276
Size: 53 83 186 936 4750 2468
Fragments arranged by size:
4750 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI PacI
PspOMI PsrI SexAI SfiI SrfI SwaI