Restriction map for
pFN21AE2308
(Circular) (Six-base) MAPSORT of: pFN21AE2308.seq Check: 2746 from: 1 to: 8719
pFN21AE2308 8719 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2590 4457 5332 2590
Size: 1867 875 5977
Fragments arranged by size:
5977 1867 875
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5312 6529 278
Size: 53 83 186 936 3776 1217 2468
Fragments arranged by size:
3776 2468 1217 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6062 1937
Size: 4125 4594
Acc65I G'GTAC_C
Cuts at: 2409 6040 2409
Size: 3631 5088
AclI AA'CG_TT
Cuts at: 1563 6846 7219 1563
Size: 5283 373 3063
Fragments arranged by size:
5283 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2630 4936 6782 8166 8434 1632
Size: 998 2306 1846 1384 268 1917
Fragments arranged by size:
2306 1917 1846 1384 998 268
AfeI AGC'GCT
Cuts at: 2141 5890 2141
Size: 3749 4970
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8522
AflIII A'CryG_T
Cuts at: 1203 4308 7618 8595 1203
Size: 3105 3310 977 1327
Fragments arranged by size:
3310 3105 1327 977
AgeI A'CCGG_T
Cuts at: 7550 7550
Size: 8719
AhdI GACnn_n'nnGTC
Cuts at: 5670 5980 7448 5670
Size: 310 1468 6941
Fragments arranged by size:
6941 1468 310
AleI CACnn'nnGTG
Cuts at: 1166 5483 6008 1166
Size: 4317 525 3877
Fragments arranged by size:
4317 3877 525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4301 1184
Size: 3117 5602
AlwNI CAG_nnn'CTG
Cuts at: 3703 5288 6462 8034 3703
Size: 1585 1174 1572 4388
Fragments arranged by size:
4388 1585 1572 1174
ApaI G_GGCC'C
Cuts at: 4545 4792 5171 4545
Size: 247 379 8093
Fragments arranged by size:
8093 379 247
ApaLI G'TGCA_C
Cuts at: 6776 7932 6776
Size: 1156 7563
ApoI r'AATT_y
Cuts at: 1493 6028 6254 6406 8515 1493
Size: 4535 226 152 2109 1697
Fragments arranged by size:
4535 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2265 2265
Size: 8719
AseI AT'TA_AT
Cuts at: 160 1032 7273 160
Size: 872 6241 1606
Fragments arranged by size:
6241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2222 3179 3370 3469 3566 3594
Size: 73 90 189 957 191 99 97 28
Cuts at: 3594 3682 3820 4214 4784 4891 4984 5027 5116
Size: 88 138 394 570 107 93 43 89
Cuts at: 5116 5170 5668 5678 5789 5873 6044 1870
Size: 54 498 10 111 84 171 4545
Fragments arranged by size:
4545 957 570 498 394 191 189 171
138 111 107 99 97 93 90 89
88 84 73 54 43 28 10
BamHI G'GATC_C
Cuts at: 6049 6416 6049
Size: 367 8352
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2203 2409 2510 2531 3140
Size: 325 217 627 416 206 101 21 609
Cuts at: 3140 3763 4337 4886 4895 4979 5174 5399 5632
Size: 623 574 549 9 84 195 225 233
Cuts at: 5632 5644 5939 6040 7495 618
Size: 12 295 101 1455 1842
Fragments arranged by size:
1842 1455 627 623 609 574 549 416
325 295 233 225 217 206 195 101
101 84 21 12 9
BanII G_rGCy'C
Cuts at: 1391 2015 2895 3170 3179 3377 3476 3932 4043
Size: 624 880 275 9 198 99 456 111
Cuts at: 4043 4211 4487 4545 4743 4751 4785 4792 4883
Size: 168 276 58 198 8 34 7 91
Cuts at: 4883 5006 5171 5852 6038 1391
Size: 123 165 681 186 4072
Fragments arranged by size:
4072 880 681 624 456 276 275 198
198 186 168 165 123 111 99 91
58 34 9 8 7
BbeI G_GCGC'C
Cuts at: 2207 2514 2535 3144 4983 5178 5403 5636 2207
Size: 307 21 609 1839 195 225 233 5290
Fragments arranged by size:
5290 1839 609 307 233 225 195 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7748
BbvCI CC'TCA_GC
Cuts at: 5832 5832
Size: 8719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6910 6944 1903
Size: 34 4973 34 3678
Fragments arranged by size:
4973 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5055 6611 7821 5055
Size: 1556 1210 5953
Fragments arranged by size:
5953 1556 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8719
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 2540 2748
Size: 107 122 71 631 605 363 505 208
Cuts at: 2748 2879 3012 3660 3751 4129 4568 4615 4638
Size: 131 133 648 91 378 439 47 23
Cuts at: 4638 4656 5297 7330 136
Size: 18 641 2033 1525
Fragments arranged by size:
2033 1525 648 641 631 605 505 439
378 363 208 133 131 122 107 91
71 47 23 18
BglII A'GATC_T
Cuts at: 4068 8714 4068
Size: 4646 4073
BlpI GC'TnA_GC
Cuts at: 5327 6136 5327
Size: 809 7910
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4340 4545 4792 4898 5171 5944 6780
Size: 1978 572 205 247 106 273 773 836
Cuts at: 6780 7936 1790
Size: 1156 2573
Fragments arranged by size:
2573 1978 1156 836 773 572 273 247
205 106
BmgBI CAC'GTC
Cuts at: 1318 5776 1318
Size: 4458 4261
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6450 7408 442
Size: 416 53 83 399 24 5033 958 1753
Fragments arranged by size:
5033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3532 3934 5053 5476 3532
Size: 402 1119 423 6775
Fragments arranged by size:
6775 1119 423 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 3433 3502 3916 4526 4541 4709 5607
Size: 1649 545 69 414 610 15 168 898
Cuts at: 5607 7379 1239
Size: 1772 2579
Fragments arranged by size:
2579 1772 1649 898 610 545 414 168
69 15
Bpu10I CC'TnA_GC
Cuts at: 3173 3200 3218 3650 4388 4882 4971 5832 3173
Size: 27 18 432 738 494 89 861 6060
Fragments arranged by size:
6060 861 738 494 432 89 27 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 6844 7709 8007 2870
Size: 3974 865 298 3582
Fragments arranged by size:
3974 3582 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2852 3685 4926 5447 5723 5759 5885
Size: 617 1353 833 1241 521 276 36 126
Cuts at: 5885 7382 882
Size: 1497 2219
Fragments arranged by size:
2219 1497 1353 1241 833 617 521 276
126 36
BsaAI yAC'GTr
Cuts at: 493 1204 4474 6510 493
Size: 711 3270 2036 2702
Fragments arranged by size:
3270 2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 8713 8713
Size: 8719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 2322 2511 2532
Size: 53 83 186 936 671 118 189 21
Cuts at: 2532 3141 3584 3974 4823 4980 5175 5309 5400
Size: 609 443 390 849 157 195 134 91
Cuts at: 5400 5633 5898 6526 6908 7543 275
Size: 233 265 628 382 635 1451
Fragments arranged by size:
1451 936 849 671 635 628 609 443
390 382 265 233 195 189 186 157
134 118 91 83 53 21
BsaWI w'CCGG_w
Cuts at: 4735 7152 7550 7824 7971 8382 4735
Size: 2417 398 274 147 411 5072
Fragments arranged by size:
5072 2417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 3275 3305 3914
Size: 30 165 30 990 30 852 30 609
Cuts at: 3914 3944 4340 4370 4940 4970 5330 5360 5513
Size: 30 396 30 570 30 360 30 153
Cuts at: 5513 5543 5834 5864 1178
Size: 30 291 30 4033
Fragments arranged by size:
4033 990 852 609 570 396 360 291
165 153 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3766 4523 4526 4831 5026 5452 1186
Size: 2580 757 3 305 195 426 4453
Fragments arranged by size:
4453 2580 757 426 305 195 3
BseYI C'CCAG_C
Cuts at: 3002 3161 3479 3768 4316 4544 4700 4709 4876
Size: 159 318 289 548 228 156 9 167
Cuts at: 4876 5802 7922 8418 3002
Size: 926 2120 496 3303
Fragments arranged by size:
3303 2120 926 548 496 318 289 228
167 159 156 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3091 3407 4352 1163
Size: 573 1355 316 945 5530
Fragments arranged by size:
5530 1355 945 573 316
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2451 2580 2793 2909 3135 3238
Size: 275 186 450 129 213 116 226 103
Cuts at: 3238 3761 4630 4660 5151 5660 6169 6930 7958
Size: 523 869 30 491 509 509 761 1028
Cuts at: 7958 1540
Size: 2301
Fragments arranged by size:
2301 1028 869 761 523 509 509 491
450 275 226 213 186 129 116 103
30
BsiHKAI G_wGCw'C
Cuts at: 2895 3932 4211 4479 4487 5852 6780 6865 7936
Size: 1037 279 268 8 1365 928 85 1071
Cuts at: 7936 2895
Size: 3678
Fragments arranged by size:
3678 1365 1071 1037 928 279 268 85
8
BsmI GAATG_Cn'
Cuts at: 6230 6323 6230
Size: 93 8626
BsmBI CGTCTCn'nnnn_
Cuts at: 2043 2451 3575 3591 5091 2043
Size: 408 1124 16 1500 5671
Fragments arranged by size:
5671 1500 1124 408 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2895 3170 3179 3377 3476 3768
Size: 399 225 880 275 9 198 99 292
Cuts at: 3768 3932 4043 4211 4340 4479 4487 4545 4743
Size: 164 111 168 129 139 8 58 198
Cuts at: 4743 4751 4785 4792 4883 4898 5006 5171 5852
Size: 8 34 7 91 15 108 165 681
Cuts at: 5852 5944 6038 6780 6865 7936 1391
Size: 92 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 681 399 292 275
225 198 198 168 165 164 139 129
111 108 99 94 92 91 85 58
34 15 9 8 8 7
BspEI T'CCGG_A
Cuts at: 8382 8382
Size: 8719
BspHI T'CATG_A
Cuts at: 2730 4479 6608 8338 2730
Size: 1749 2129 1730 3111
Fragments arranged by size:
3111 2129 1749 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2590 3003 3540 4457 5332 6074 844
Size: 1277 469 413 537 917 875 742 3489
Fragments arranged by size:
3489 1277 917 875 742 537 469 413
BsrBI CCG'CTC
Cuts at: 1394 1823 2046 2787 3027 3128 3207 3222 3291
Size: 429 223 741 240 101 79 15 69
Cuts at: 3291 3666 4005 4387 4428 4927 4989 5706 6606
Size: 375 339 382 41 499 62 717 900
Cuts at: 6606 1394
Size: 3507
Fragments arranged by size:
3507 900 741 717 499 429 382 375
339 240 223 101 79 69 62 41
15
BsrDI GCAATG_nn'
Cuts at: 65 1248 7214 7388 65
Size: 1183 5966 174 1396
Fragments arranged by size:
5966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2038 2528 3058 3148 3184 3262 3760
Size: 110 228 490 530 90 36 78 498
Cuts at: 3760 4571 4911 5110 5629 5655 5738 5909 7363
Size: 811 340 199 519 26 83 171 1454
Cuts at: 7363 7550 1700
Size: 187 2869
Fragments arranged by size:
2869 1454 811 530 519 498 490 340
228 199 187 171 110 90 83 78
36 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8719
BssHII G'CGCG_C
Cuts at: 1925 2265 2314 2415 2672 2967 2969 3190 3192
Size: 340 49 101 257 295 2 221 2
Cuts at: 3192 3273 3535 1925
Size: 81 262 7109
Fragments arranged by size:
7109 340 295 262 257 221 101 81
49 2 2
BssSI C'ACGA_G
Cuts at: 2855 3076 4718 6779 7791 2855
Size: 221 1642 2061 1012 3783
Fragments arranged by size:
3783 2061 1642 1012 221
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3078 3421 4072 4242 794
Size: 2284 343 651 170 5271
Fragments arranged by size:
5271 2284 651 343 170
BstBI TT'CG_AA
Cuts at: 7523 7523
Size: 8719
BstEII G'GTnAC_C
Cuts at: 1184 4327 5476 1184
Size: 3143 1149 4427
Fragments arranged by size:
4427 3143 1149
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 3307 2623
Size: 684 8035
BstYI r'GATC_y
Cuts at: 1972 4068 6049 6416 6801 6818 8270 8356 8368
Size: 2096 1981 367 385 17 1452 86 12
Cuts at: 8368 8714 1972
Size: 346 1977
Fragments arranged by size:
2096 1981 1977 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4527 4527
Size: 8719
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2216 2373 2574 3135 3238
Size: 552 939 14 198 157 201 561 103
Cuts at: 3238 3306 3364 3427 3636 4260 4332 4491 4998
Size: 68 58 63 209 624 72 159 507
Cuts at: 4998 5395 5532 5660 513
Size: 397 137 128 3572
Fragments arranged by size:
3572 939 624 561 552 507 397 209
201 198 159 157 137 128 103 72
68 63 58 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3559 5749 487
Size: 683 56 2333 2190 3457
Fragments arranged by size:
3457 2333 2190 683 56
BtsI GCAGTG_nn'
Cuts at: 4003 4727 6235 7029 7049 4003
Size: 724 1508 794 20 5673
Fragments arranged by size:
5673 1508 794 724 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5604 5639 529
Size: 35 5040 35 3609
Fragments arranged by size:
5040 3609 35 35
DraI TTT'AAA
Cuts at: 6023 6023
Size: 8719
DraIII CAC_nnn'GTG
Cuts at: 2095 4999 2095
Size: 2904 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7726 809
Size: 507 6410 1802
Fragments arranged by size:
6410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2376 2448
Size: 54 1425 50 275 18 301 245 72
Cuts at: 2448 2577 2749 2790 2906 2940 3045 3063 3132
Size: 129 172 41 116 34 105 18 69
Cuts at: 3132 3235 3361 3436 3661 3758 4554 4627 4657
Size: 103 126 75 225 97 796 73 30
Cuts at: 4657 4990 5148 5241 5298 5657 6166 7055 8684
Size: 333 158 93 57 359 509 889 1629
Cuts at: 8684 8
Size: 43
Fragments arranged by size:
1629 1425 889 796 509 359 333 301
275 245 225 172 158 129 126 116
105 103 97 93 75 73 72 69
57 54 50 43 41 34 30 18
18
EagI C'GGCC_G
Cuts at: 1537 1812 2448 2577 2790 2906 3132 3235 3758
Size: 275 636 129 213 116 226 103 523
Cuts at: 3758 4627 4657 5148 5657 6166 1537
Size: 869 30 491 509 509 4090
Fragments arranged by size:
4090 869 636 523 509 509 491 275
226 213 129 116 103 30
EarI CTCTTCn'nnn_
Cuts at: 1353 2900 5315 6649 1353
Size: 1547 2415 1334 3423
Fragments arranged by size:
3423 2415 1547 1334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2266 2729 2840 3454 4045 4843
Size: 1064 138 677 463 111 614 591 798
Cuts at: 4843 5037 7306 7680 7826 387
Size: 194 2269 374 146 1280
Fragments arranged by size:
2269 1280 1064 798 677 614 591 463
374 194 146 138 111
EcoICRI GAG'CTC
Cuts at: 2893 3930 4209 4485 5850 2893
Size: 1037 279 276 1365 5762
Fragments arranged by size:
5762 1365 1037 279 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2630 2888 3433 3502 3916 4526 4541
Size: 393 998 258 545 69 414 610 15
Cuts at: 4541 4709 4936 5607 6782 7379 8166 8434 1239
Size: 168 227 671 1175 597 787 268 1524
Fragments arranged by size:
1524 1175 998 787 671 610 597 545
414 393 268 258 227 168 69 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4508 4508
Size: 8719
EcoO109I rG'GnC_Cy
Cuts at: 2801 3645 3700 3726 4121 4438 4541 4788 5167
Size: 844 55 26 395 317 103 247 379
Cuts at: 5167 5933 2801
Size: 766 5587
Fragments arranged by size:
5587 844 766 395 379 317 247 103
55 26
EcoRI G'AATT_C
Cuts at: 6028 6406 6028
Size: 378 8341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2911 4087 2161
Size: 750 1176 6793
Fragments arranged by size:
6793 1176 750
FseI GG_CCGG'CC
Cuts at: 4575 5659 4575
Size: 1084 7635
FspI TGC'GCA
Cuts at: 2099 2117 3089 3719 4235 5723 7225 2099
Size: 18 972 630 516 1488 1502 3593
Fragments arranged by size:
3593 1502 1488 972 630 516 18
FspAI rTGC'GCAy
Cuts at: 3089 4235 3089
Size: 1146 7573
HaeII r_GCGC'y
Cuts at: 1678 2143 2207 2499 2514 2535 2885 3144 3257
Size: 465 64 292 15 21 350 259 113
Cuts at: 3257 3462 4563 4983 5178 5403 5518 5636 5892
Size: 205 1101 420 195 225 115 118 256
Cuts at: 5892 7866 1678
Size: 1974 2531
Fragments arranged by size:
2531 1974 1101 465 420 350 292 259
256 225 205 195 118 115 113 64
21 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2696 2728 4340 4372 4940
Size: 32 135 32 1124 32 1612 32 568
Cuts at: 4940 4972 5330 5362 5656 5688 5834 5866 5966
Size: 32 358 32 294 32 146 32 100
Cuts at: 5966 5998 7360 7392 7434 7466 1373
Size: 32 1362 32 42 32 2626
Fragments arranged by size:
2626 1612 1362 1124 568 358 294 146
135 100 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2992 6063 6309 669
Size: 988 281 1054 3071 246 3079
Fragments arranged by size:
3079 3071 1054 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8719
HpaI GTT'AAC
Cuts at: 6309 6309
Size: 8719
KasI G'GCGC_C
Cuts at: 2203 2510 2531 3140 4979 5174 5399 5632 2203
Size: 307 21 609 1839 195 225 233 5290
Fragments arranged by size:
5290 1839 609 307 233 225 195 21
KpnI G_GTAC'C
Cuts at: 2413 6044 2413
Size: 3631 5088
MfeI C'AATT_G
Cuts at: 6318 6318
Size: 8719
MluI A'CGCG_T
Cuts at: 8595 8595
Size: 8719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 7833 8017 1305
Size: 3651 2877 184 2007
Fragments arranged by size:
3651 2877 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2133 5300 10
Size: 54 1425 343 301 3167 3429
Fragments arranged by size:
3429 3167 1425 343 301 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2777 3299 3305 3635 3803 3968 4034
Size: 648 1611 522 6 330 168 165 66
Cuts at: 4034 4235 4272 5225 5483 5774 6008 6677 7036
Size: 201 37 953 258 291 234 669 359
Cuts at: 7036 7195 518
Size: 159 2042
Fragments arranged by size:
2042 1611 953 669 648 522 359 330
291 258 234 201 168 165 159 66
37 6
MspA1I CmG'CkG
Cuts at: 1606 2020 2218 2745 3137 3240 3360 3421 3442
Size: 414 198 527 392 103 120 61 21
Cuts at: 3442 3453 3757 4011 4918 5397 6135 6812 7960
Size: 11 304 254 907 479 738 677 1148
Cuts at: 7960 8205 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 907 738 677 527 479 414
392 304 254 245 198 120 103 61
21 11
NaeI GCC'GGC
Cuts at: 2040 2530 3060 3264 4573 4913 5112 5631 5657
Size: 490 530 204 1309 340 199 519 26
Cuts at: 5657 5740 2040
Size: 83 5019
Fragments arranged by size:
5019 1309 530 519 490 340 204 199
83 26
NarI GG'CG_CC
Cuts at: 2204 2511 2532 3141 4980 5175 5400 5633 2204
Size: 307 21 609 1839 195 225 233 5290
Fragments arranged by size:
5290 1839 609 307 233 225 195 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 3636 4260 513
Size: 552 939 1632 624 4972
Fragments arranged by size:
4972 1632 939 624 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8719
NgoMIV G'CCGG_C
Cuts at: 2038 2528 3058 3262 4571 4911 5110 5629 5655
Size: 490 530 204 1309 340 199 519 26
Cuts at: 5655 5738 2038
Size: 83 5019
Fragments arranged by size:
5019 1309 530 519 490 340 204 199
83 26
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8719
NotI GC'GGCC_GC
Cuts at: 2448 3132 3235 4627 6166 2448
Size: 684 103 1392 1539 5001
Fragments arranged by size:
5001 1539 1392 684 103
NspI r_CATG'y
Cuts at: 1132 1229 3967 4033 4234 4240 4271 4717 6077
Size: 97 2738 66 201 6 31 446 1360
Cuts at: 6077 7622 1132
Size: 1545 2229
Fragments arranged by size:
2738 2229 1545 1360 446 201 97 66
31 6
PasI CC'CwG_GG
Cuts at: 1348 2515 4080 5164 1348
Size: 1167 1565 1084 4903
Fragments arranged by size:
4903 1565 1167 1084
PciI A'CATG_T
Cuts at: 7618 7618
Size: 8719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2986 3703 4999 1108
Size: 1878 717 1296 4828
Fragments arranged by size:
4828 1878 1296 717
PfoI T'CCnGG_A
Cuts at: 2865 4503 2865
Size: 1638 7081
PmeI GTTT'AAAC
Cuts at: 6023 6023
Size: 8719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6788 6820 8301 8333 6788
Size: 32 1481 32 7174
Fragments arranged by size:
7174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3700 3726 5933 3700
Size: 26 2207 6486
Fragments arranged by size:
6486 2207 26
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8719
PsiI TTA'TAA
Cuts at: 6289 6289
Size: 8719
PspOMI G'GGCC_C
Cuts at: 4541 4788 5167 4541
Size: 247 379 8093
Fragments arranged by size:
8093 379 247
PspXI vC'TCGA_Gb
Cuts at: 2222 2222
Size: 8719
PstI C_TGCA'G
Cuts at: 830 2495 2727 3548 4016 5094 5370 6071 830
Size: 1665 232 821 468 1078 276 701 3478
Fragments arranged by size:
3478 1665 1078 821 701 468 276 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8719
PvuII CAG'CTG
Cuts at: 4011 4011
Size: 8719
SacI G_AGCT'C
Cuts at: 2895 3932 4211 4487 5852 2895
Size: 1037 279 276 1365 5762
Fragments arranged by size:
5762 1365 1037 279 276
SacII CC_GC'GG
Cuts at: 2021 2219 3138 3241 5398 2021
Size: 198 919 103 2157 5342
Fragments arranged by size:
5342 2157 919 198 103
SalI G'TCGA_C
Cuts at: 1936 6061 1936
Size: 4125 4594
SanDI GG'GwC_CC
Cuts at: 5933 5933
Size: 8719
SapI GCTCTTCn'nnn_
Cuts at: 2900 2900
Size: 8719
SbfI CC_TGCA'GG
Cuts at: 5370 6071 5370
Size: 701 8018
ScaI AGT'ACT
Cuts at: 2228 2366 6967 8679 2228
Size: 138 4601 1712 2268
Fragments arranged by size:
4601 2268 1712 138
SexAI A'CCwGG_T
Cuts at: 2427 2985 2427
Size: 558 8161
SfcI C'TryA_G
Cuts at: 826 1045 2491 2723 3544 4012 4411 4596 5090
Size: 219 1446 232 821 468 399 185 494
Cuts at: 5090 5366 6067 7202 7883 8074 826
Size: 276 701 1135 681 191 1471
Fragments arranged by size:
1471 1446 1135 821 701 681 494 468
399 276 232 219 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2035 4129 4656 2035
Size: 2094 527 6098
Fragments arranged by size:
6098 2094 527
SfoI GGC'GCC
Cuts at: 2205 2512 2533 3142 4981 5176 5401 5634 2205
Size: 307 21 609 1839 195 225 233 5290
Fragments arranged by size:
5290 1839 609 307 233 225 195 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8719
SgrAI Cr'CCGG_yG
Cuts at: 1700 3058 5909 1700
Size: 1358 2851 4510
Fragments arranged by size:
4510 2851 1358
SmaI CCC'GGG
Cuts at: 1872 2035 3181 4786 4893 5118 5172 5670 5680
Size: 163 1146 1605 107 225 54 498 10
Cuts at: 5680 5791 6046 1872
Size: 111 255 4545
Fragments arranged by size:
4545 1605 1146 498 255 225 163 111
107 54 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2885 6823 7724 7986 820
Size: 197 926 279 663 3938 901 262 1553
Fragments arranged by size:
3938 1553 926 901 663 279 262 197
SnaBI TAC'GTA
Cuts at: 493 6510 493
Size: 6017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8719
SphI G_CATG'C
Cuts at: 1132 4271 4717 6077 1132
Size: 3139 446 1360 3774
Fragments arranged by size:
3774 3139 1360 446
SrfI GCCC'GGGC
Cuts at: 2035 5172 2035
Size: 3137 5582
SspI AAT'ATT
Cuts at: 5 52 6643 5
Size: 47 6591 2081
Fragments arranged by size:
6591 2081 47
StuI AGG'CCT
Cuts at: 1517 5033 5360 1517
Size: 3516 327 4876
Fragments arranged by size:
4876 3516 327
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 2616 3636 3867 4260 5007
Size: 552 939 351 261 1020 231 393 747
Cuts at: 5007 5403 6158 513
Size: 396 755 3074
Fragments arranged by size:
3074 1020 939 755 747 552 396 393
351 261 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3723 4153 5564 6746 6763 6916 7101
Size: 1255 2143 430 1411 1182 17 153 185
Cuts at: 7101 7546 325
Size: 445 1498
Fragments arranged by size:
2143 1498 1411 1255 1182 445 430 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2364 4758
Size: 274 80 33 51 1443 249 138 2394
Cuts at: 4758 6965 8677 96
Size: 2207 1712 138
Fragments arranged by size:
2394 2207 1712 1443 274 249 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3275 3307 3914 3946 8679 8711 3275
Size: 32 607 32 4733 32 3283
Fragments arranged by size:
4733 3283 607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 4325 1316
Size: 1113 1896 5710
Fragments arranged by size:
5710 1896 1113
XbaI T'CTAG_A
Cuts at: 6055 6055
Size: 8719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3940 4258 3940
Size: 318 8401
XhoI C'TCGA_G
Cuts at: 1943 2222 1943
Size: 279 8440
XmaI C'CCGG_G
Cuts at: 1870 2033 3179 4784 4891 5116 5170 5668 5678
Size: 163 1146 1605 107 225 54 498 10
Cuts at: 5678 5789 6044 1870
Size: 111 255 4545
Fragments arranged by size:
4545 1605 1146 498 255 225 163 111
107 54 10
XmnI GAAnn'nnTTC
Cuts at: 6848 6848
Size: 8719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5310 6527 276
Size: 53 83 186 936 3776 1217 2468
Fragments arranged by size:
3776 2468 1217 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV NruI
NsiI PacI PmlI PsrI RsrII SwaI