Restriction map for
pFN21AE2306
(Circular) (Six-base) MAPSORT of: pFN21AE2306.seq Check: 2342 from: 1 to: 5596
pFN21AE2306 5596 bp
With 182 enzymes: SgfI *
November 16, 2010 14:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2625 2917 2625
Size: 292 5304
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 3659 5043 5311 1632
Size: 731 1296 1384 268 1917
Fragments arranged by size:
1917 1384 1296 731 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 4495 5472 1203
Size: 3292 977 1327
Fragments arranged by size:
3292 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2715 4325 2715
Size: 1610 3986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 2209 2278 2482 3339 4911 2209
Size: 69 204 857 1572 2894
Fragments arranged by size:
2894 1572 857 204 69
ApaI G_GGCC'C
Cuts at: 2840 2840
Size: 5596
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2066 2369 2905 3131 3283 5392 1493
Size: 573 303 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 573 536 303 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2285 2609 2703 2921 1870
Size: 73 342 324 94 218 4545
Fragments arranged by size:
4545 342 324 218 94 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2625 2917 4372 618
Size: 325 217 627 245 593 292 1455 1842
Fragments arranged by size:
1842 1455 627 593 325 292 245 217
BanII G_rGCy'C
Cuts at: 1391 2250 2318 2609 2840 2880 2915 1391
Size: 859 68 291 231 40 35 4072
Fragments arranged by size:
4072 859 291 231 68 40 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BbvCI CC'TCA_GC
Cuts at: 2426 2426
Size: 5596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2582 3488 4698 2582
Size: 906 1210 3480
Fragments arranged by size:
3480 1210 906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2047 2168 4207
Size: 107 122 71 631 605 375 121 2039
Cuts at: 4207 136
Size: 1525
Fragments arranged by size:
2039 1525 631 605 375 122 121 107
71
BglII A'GATC_T
Cuts at: 2812 5591 2812
Size: 2779 2817
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2234 2364 2419 2840 3657 4813 1790
Size: 247 197 130 55 421 817 1156 2573
Fragments arranged by size:
2573 1156 817 421 247 197 130 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2124 2623 2651 4256 1239
Size: 885 499 28 1605 2579
Fragments arranged by size:
2579 1605 885 499 28
Bpu10I CC'TnA_GC
Cuts at: 2426 2426
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 3721 4586 4884 2534
Size: 1187 865 298 3246
Fragments arranged by size:
3246 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2627 4259 882
Size: 617 1128 1632 2219
Fragments arranged by size:
2219 1632 1128 617
BsaAI yAC'GTr
Cuts at: 493 1204 2229 3387 493
Size: 711 1025 1158 2702
Fragments arranged by size:
2702 1158 1025 711
BsaBI GATnn'nnATC
Cuts at: 2570 5590 2570
Size: 3020 2576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2264 3403 3785 4420
Size: 53 83 186 936 731 1139 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 1139 936 731 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2684 2714 1178
Size: 30 165 30 1281 30 4060
Fragments arranged by size:
4060 1281 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5596
BseYI C'CCAG_C
Cuts at: 2037 2219 2398 2538 2618 4799 5295 2037
Size: 182 179 140 80 2181 496 2338
Fragments arranged by size:
2338 2181 496 182 179 140 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2569 2855 1163
Size: 573 696 137 286 3904
Fragments arranged by size:
3904 696 573 286 137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2411 3657 3742 4813 2411
Size: 1246 85 1071 3194
Fragments arranged by size:
3194 1246 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3200 3107
Size: 93 5503
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2704 2255
Size: 449 5147
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2234 2250 2318 2364 2411 2419
Size: 399 247 197 16 68 46 47 8
Cuts at: 2419 2609 2840 2880 2915 3657 3742 4813 1391
Size: 190 231 40 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 247 231 197 190
85 68 47 46 40 35 16 8
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 2951 844
Size: 1610 497 3489
Fragments arranged by size:
3489 1610 497
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 2873 3656 4668 2873
Size: 783 1012 3801
Fragments arranged by size:
3801 1012 783
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5596
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2533 2777 2812 2926 3293 3678 3695 5147
Size: 561 244 35 114 367 385 17 1452
Cuts at: 5147 5233 5245 5591 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 561 385 367 346 244 114
86 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2044 2044
Size: 5596
BtgI C'CryG_G
Cuts at: 513 1065 2004 2464 513
Size: 552 939 460 3645
Fragments arranged by size:
3645 939 552 460
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2779 487
Size: 683 56 1553 3304
Fragments arranged by size:
3304 1553 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5561
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2577 3043
Size: 54 1425 50 275 18 279 468 466
Cuts at: 3043 3932 5561 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 468 466 279 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2363 2623 2651 3659 4256 5043
Size: 393 492 239 260 28 1008 597 787
Cuts at: 5043 5311 1239
Size: 268 1524
Fragments arranged by size:
1524 1008 787 597 492 393 268 260
239 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2211 2084
Size: 127 5469
EcoO109I rG'GnC_Cy
Cuts at: 2095 2275 2506 2545 2837 2095
Size: 180 231 39 292 4854
Fragments arranged by size:
4854 292 231 180 39
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 2354 4102 2354
Size: 1748 3848
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2317 2349 2684 2701 2716
Size: 32 135 32 745 32 335 17 15
Cuts at: 2716 2733 4237 4269 4311 4343 1373
Size: 17 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 745 335 135 42 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 2940 3186 669
Size: 988 281 802 200 246 3079
Fragments arranged by size:
3079 988 802 281 246 200
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2629 2921 2629
Size: 292 5304
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 4710 4894 1305
Size: 1017 2388 184 2007
Fragments arranged by size:
2388 2007 1017 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 10
Size: 54 1425 343 279 3495
Fragments arranged by size:
3495 1425 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2309 3554 3913 4072 518
Size: 648 1143 1245 359 159 2042
Fragments arranged by size:
2042 1245 1143 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2663 3012 3689 4837 5082 1606
Size: 1057 349 677 1148 245 2120
Fragments arranged by size:
2120 1148 1057 677 349 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2464 513
Size: 552 939 460 3645
Fragments arranged by size:
3645 939 552 460
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2954 4499 1132
Size: 97 1725 1545 2229
Fragments arranged by size:
2229 1725 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2012 2364 1348
Size: 664 352 4580
Fragments arranged by size:
4580 664 352
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2101 2101
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PmlI CAC'GTG
Cuts at: 2229 2229
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2506 2095
Size: 411 5185
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PspOMI G'GGCC_C
Cuts at: 2836 2836
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2478 2948 830
Size: 1648 470 3478
Fragments arranged by size:
3478 1648 470
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2474 2944 4079 4760 4951 826
Size: 219 1429 470 1135 681 191 1471
Fragments arranged by size:
1471 1429 1135 681 470 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2168 2168
Size: 5596
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2287 2705 2923 1872
Size: 415 418 218 4545
Fragments arranged by size:
4545 418 415 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 3700 4601 4863 820
Size: 197 926 606 1151 901 262 1553
Fragments arranged by size:
1553 1151 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 2832 1517
Size: 1128 187 4281
Fragments arranged by size:
4281 1128 187
StyI C'CwwG_G
Cuts at: 513 1065 2004 2464 3035 513
Size: 552 939 460 571 3074
Fragments arranged by size:
3074 939 571 552 460
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2466 3623 3640 3793 3978 4423 325
Size: 1255 886 1157 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1157 886 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 2696 1316
Size: 1380 4216
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2285 2703 2921 1870
Size: 415 418 218 4545
Fragments arranged by size:
4545 418 415 218
XmnI GAAnn'nnTTC
Cuts at: 2492 3725 2492
Size: 1233 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI XcmI