Restriction map for
pFN21AE2305
(Circular) (Six-base) MAPSORT of: pFN21AE2305.seq Check: 2056 from: 1 to: 14641
pFN21AE2305 14641 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3652 6479 7925 8007 8721 3652
Size: 2827 1446 82 714 9572
Fragments arranged by size:
9572 2827 1446 714 82
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5934 12451 278
Size: 53 83 186 936 4398 6517 2468
Fragments arranged by size:
6517 4398 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2048 3087 4347 11984 1937
Size: 111 1039 1260 7637 4594
Fragments arranged by size:
7637 4594 1260 1039 111
Acc65I G'GTAC_C
Cuts at: 7365 11962 7365
Size: 4597 10044
AclI AA'CG_TT
Cuts at: 1563 12768 13141 1563
Size: 11205 373 3063
Fragments arranged by size:
11205 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2505 2931 7219 7694 9150 10719 11057 12704
Size: 873 426 4288 475 1456 1569 338 1647
Cuts at: 12704 14088 14356 1632
Size: 1384 268 1917
Fragments arranged by size:
4288 1917 1647 1569 1456 1384 873 475
426 338 268
AfeI AGC'GCT
Cuts at: 3650 5861 7223 3650
Size: 2211 1362 11068
Fragments arranged by size:
11068 2211 1362
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 14444
AflIII A'CryG_T
Cuts at: 1203 3040 5847 6135 6375 13540 14517 1203
Size: 1837 2807 288 240 7165 977 1327
Fragments arranged by size:
7165 2807 1837 1327 977 288 240
AgeI A'CCGG_T
Cuts at: 11043 13472 11043
Size: 2429 12212
AhdI GACnn_n'nnGTC
Cuts at: 3229 4153 10849 11584 13370 3229
Size: 924 6696 735 1786 4500
Fragments arranged by size:
6696 4500 1786 924 735
AleI CACnn'nnGTG
Cuts at: 1166 3032 3257 3692 4325 4634 5276 7179 8058
Size: 1866 225 435 633 309 642 1903 879
Cuts at: 8058 9089 9635 1166
Size: 1031 546 6172
Fragments arranged by size:
6172 1903 1866 1031 879 642 633 546
435 309 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3050 3387 5666 6951 9517 11381 1184
Size: 1866 337 2279 1285 2566 1864 4444
Fragments arranged by size:
4444 2566 2279 1866 1864 1285 337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2331 2363 2331
Size: 32 14609
AlwNI CAG_nnn'CTG
Cuts at: 2396 2771 3001 4961 5659 7672 7738 8367 9212
Size: 375 230 1960 698 2013 66 629 845
Cuts at: 9212 9877 10297 10382 10403 10592 11846 12384 13956
Size: 665 420 85 21 189 1254 538 1572
Cuts at: 13956 2396
Size: 3081
Fragments arranged by size:
3081 2013 1960 1572 1254 845 698 665
629 538 420 375 230 189 85 66
21
ApaI G_GGCC'C
Cuts at: 2307 6039 6536 6869 7013 7866 8473 8857 9025
Size: 3732 497 333 144 853 607 384 168
Cuts at: 9025 11014 11813 11859 2307
Size: 1989 799 46 5089
Fragments arranged by size:
5089 3732 1989 853 799 607 497 384
333 168 144 46
ApaLI G'TGCA_C
Cuts at: 3743 4535 5468 6858 8271 9512 9808 12698 13854
Size: 792 933 1390 1413 1241 296 2890 1156
Cuts at: 13854 3743
Size: 4530
Fragments arranged by size:
4530 2890 1413 1390 1241 1156 933 792
296
ApoI r'AATT_y
Cuts at: 1493 11950 12176 12328 14437 1493
Size: 10457 226 152 2109 1697
Fragments arranged by size:
10457 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 13195 160
Size: 872 12163 1606
Fragments arranged by size:
12163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14641
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2491 2497 2719 2732 2810 2838
Size: 73 93 455 6 222 13 78 28
Cuts at: 2838 2876 2890 3569 4188 4803 5891 7043 7492
Size: 38 14 679 619 615 1088 1152 449
Cuts at: 7492 8463 9015 10338 11073 11385 11451 11490 11604
Size: 971 552 1323 735 312 66 39 114
Cuts at: 11604 11966 1870
Size: 362 4545
Fragments arranged by size:
4545 1323 1152 1088 971 735 679 619
615 552 455 449 362 312 222 114
93 78 73 66 39 38 28 14
13 6
AvrII C'CTAG_G
Cuts at: 6850 6850
Size: 14641
BamHI G'GATC_C
Cuts at: 9076 9723 11971 12338 9076
Size: 647 2248 367 11379
Fragments arranged by size:
11379 2248 647 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 2409 2538 2603 2818
Size: 325 217 627 331 291 129 65 215
Cuts at: 2818 3004 3026 3078 3820 4084 4319 5972 6467
Size: 186 22 52 742 264 235 1653 495
Cuts at: 6467 6521 6672 7145 7365 7995 8052 8892 9293
Size: 54 151 473 220 630 57 840 401
Cuts at: 9293 9923 10475 10920 11062 11078 11097 11399 11446
Size: 630 552 445 142 16 19 302 47
Cuts at: 11446 11623 11634 11962 13417 618
Size: 177 11 328 1455 1842
Fragments arranged by size:
1842 1653 1455 840 742 630 630 627
552 495 473 445 401 331 328 325
302 291 264 235 220 217 215 186
177 151 142 129 65 57 54 52
47 22 19 16 11
BanII G_rGCy'C
Cuts at: 1391 2268 2307 2476 2498 5046 5255 6039 6153
Size: 877 39 169 22 2548 209 784 114
Cuts at: 6153 6312 6536 6869 7013 7493 7866 8011 8108
Size: 159 224 333 144 480 373 145 97
Cuts at: 8108 8255 8311 8473 8523 8562 8857 8867 9025
Size: 147 56 162 50 39 295 10 158
Cuts at: 9025 9765 10047 10230 10913 11014 11346 11597 11813
Size: 740 282 183 683 101 332 251 216
Cuts at: 11813 11859 11960 1391
Size: 46 101 4072
Fragments arranged by size:
4072 2548 877 784 740 683 480 373
333 332 295 282 251 224 216 209
183 169 162 159 158 147 145 144
114 101 101 97 56 50 46 39
39 22 10
BbeI G_GCGC'C
Cuts at: 2413 2822 3082 6525 6676 9927 10479 10924 11403
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 11403 2413
Size: 5651
Fragments arranged by size:
5651 3443 3251 552 479 445 409 260
151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2196 2699 5194 5926 10163 928
Size: 971 297 503 2495 732 4237 5406
Fragments arranged by size:
5406 4237 2495 971 732 503 297
BbvCI CC'TCA_GC
Cuts at: 2090 4219 5211 5925 9845 2090
Size: 2129 992 714 3920 6886
Fragments arranged by size:
6886 3920 2129 992 714
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6533 6567 9427 9461 9799 9833 11366
Size: 34 4596 34 2860 34 338 34 1533
Cuts at: 11366 11400 12832 12866 1903
Size: 34 1432 34 3678
Fragments arranged by size:
4596 3678 2860 1533 1432 338 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5171 5554 7069 8599 12533 13743 5171
Size: 383 1515 1530 3934 1210 6069
Fragments arranged by size:
6069 3934 1530 1515 1210 383
BclI T'GATC_A
Cuts at: 1548 4522 1548
Size: 2974 11667
BfrBI ATG'CAT
Cuts at: 11316 11316
Size: 14641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2026 3071
Size: 107 122 71 631 605 337 17 1045
Cuts at: 3071 3801 6516 7254 7593 8091 9737 10293 10542
Size: 730 2715 738 339 498 1646 556 249
Cuts at: 10542 11374 11569 11632 13252 136
Size: 832 195 63 1620 1525
Fragments arranged by size:
2715 1646 1620 1525 1045 832 738 730
631 605 556 498 339 337 249 195
122 107 71 63 17
BglII A'GATC_T
Cuts at: 4950 8405 14636 4950
Size: 3455 6231 4955
Fragments arranged by size:
6231 4955 3455
BlpI GC'TnA_GC
Cuts at: 2140 6893 8631 8981 9689 10585 12058 2140
Size: 4753 1738 350 708 896 1473 4723
Fragments arranged by size:
4753 4723 1738 1473 896 708 350
Bme1580I G_kGCm'C
Cuts at: 1790 2121 2307 2606 2944 3747 4322 4516 4539
Size: 331 186 299 338 803 575 194 23
Cuts at: 4539 4588 5139 5472 5975 6039 6470 6512 6536
Size: 49 551 333 503 64 431 42 24
Cuts at: 6536 6862 6869 7013 7866 8055 8087 8275 8294
Size: 326 7 144 853 189 32 188 19
Cuts at: 8294 8457 8473 8857 9025 9516 9812 10507 11014
Size: 163 16 384 168 491 296 695 507
Cuts at: 11014 11083 11171 11449 11813 11859 12702 13858 1790
Size: 69 88 278 364 46 843 1156 2573
Fragments arranged by size:
2573 1156 853 843 803 695 575 551
507 503 491 431 384 364 338 333
331 326 299 296 278 194 189 188
186 168 163 144 88 69 64 49
46 42 32 24 23 19 16 7
BmgBI CAC'GTC
Cuts at: 1318 4663 4864 5473 10645 11880 1318
Size: 3345 201 609 5172 1235 4079
Fragments arranged by size:
5172 4079 3345 1235 609 201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4705 6564 9000
Size: 416 53 83 399 24 3288 1859 2436
Cuts at: 9000 9100 9265 12372 13330 442
Size: 100 165 3107 958 1753
Fragments arranged by size:
3288 3107 2436 1859 1753 958 416 399
165 100 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6005 1056
Size: 4949 9692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2784 5065 11953 2440
Size: 344 2281 6888 5128
Fragments arranged by size:
6888 5128 2281 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3143 4715 5136 5388 7583 8537 8918 9401
Size: 1904 1572 421 252 2195 954 381 483
Cuts at: 9401 9620 9632 10075 10274 10598 11360 13301 1239
Size: 219 12 443 199 324 762 1941 2579
Fragments arranged by size:
2579 2195 1941 1904 1572 954 762 483
443 421 381 324 252 219 199 12
Bpu10I CC'TnA_GC
Cuts at: 2090 2185 2310 3051 4219 5211 5340 5925 9845
Size: 95 125 741 1168 992 129 585 3920
Cuts at: 9845 11085 11291 2090
Size: 1240 206 5440
Fragments arranged by size:
5440 3920 1240 1168 992 741 585 206
129 125 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5861 6808 9157 9689 11530 12766 13631 13929 5861
Size: 947 2349 532 1841 1236 865 298 6573
Fragments arranged by size:
6573 2349 1841 1236 947 865 532 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2425 2849 3317 4132 4185 4918 6296
Size: 617 926 424 468 815 53 733 1378
Cuts at: 6296 7618 13304 882
Size: 1322 5686 2219
Fragments arranged by size:
5686 2219 1378 1322 926 815 733 617
468 424 53
BsaAI yAC'GTr
Cuts at: 493 1204 3655 6011 6076 6378 7693 9637 12432
Size: 711 2451 2356 65 302 1315 1944 2795
Cuts at: 12432 493
Size: 2702
Fragments arranged by size:
2795 2702 2451 2356 1944 1315 711 302
65
BsaBI GATnn'nnATC
Cuts at: 14635 14635
Size: 14641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 2410 2819 3063
Size: 53 83 186 936 813 64 409 244
Cuts at: 3063 3079 3729 5931 6450 6522 6673 8439 8966
Size: 16 650 2202 519 72 151 1766 527
Cuts at: 8966 9924 9957 10476 10921 11400 12448 12830 13465
Size: 958 33 519 445 479 1048 382 635
Cuts at: 13465 275
Size: 1451
Fragments arranged by size:
2202 1766 1451 1048 958 936 813 650
635 527 519 519 479 445 409 382
244 186 151 83 72 64 53 33
16
BsaWI w'CCGG_w
Cuts at: 2197 2672 5145 6401 11043 13074 13472 13746 13893
Size: 475 2473 1256 4642 2031 398 274 147
Cuts at: 13893 14304 2197
Size: 411 2534
Fragments arranged by size:
4642 2534 2473 2031 1256 475 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2049 2079 2632 2662 5428
Size: 30 165 30 646 30 553 30 2766
Cuts at: 5428 5458 6056 6086 8525 8555 11458 11488 1178
Size: 30 598 30 2439 30 2903 30 4331
Fragments arranged by size:
4331 2903 2766 2439 646 598 553 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2114 2339 2413 3359 3621 5228 5822
Size: 877 51 225 74 946 262 1607 594
Cuts at: 5822 6068 7719 7736 8915 9224 9755 10100 10400
Size: 246 1651 17 1179 309 531 345 300
Cuts at: 10400 10460 10480 10721 10724 10729 10802 11219 11758
Size: 60 20 241 3 5 73 417 539
Cuts at: 11758 11770 1186
Size: 12 4057
Fragments arranged by size:
4057 1651 1607 1179 946 877 594 539
531 417 345 309 300 262 246 241
225 74 73 60 51 20 17 12
5 3
BseYI C'CCAG_C
Cuts at: 2104 2127 2329 2565 2576 4122 5206 5590 5878
Size: 23 202 236 11 1546 1084 384 288
Cuts at: 5878 7224 7424 7805 8842 9775 9951 9973 10408
Size: 1346 200 381 1037 933 176 22 435
Cuts at: 10408 11013 11532 11705 13844 14340 2104
Size: 605 519 173 2139 496 2405
Fragments arranged by size:
2405 2139 1546 1346 1084 1037 933 605
519 496 435 384 381 288 236 202
200 176 173 23 22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5195 5450 5744 6457 6662 7079 7439
Size: 573 3459 255 294 713 205 417 360
Cuts at: 7439 7737 8787 9467 9815 9863 9890 10149 11124
Size: 298 1050 680 348 48 27 259 975
Cuts at: 11124 11310 1163
Size: 186 4494
Fragments arranged by size:
4494 3459 1050 975 713 680 573 417
360 348 298 294 259 255 205 186
48 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 2048 3280 3502 4571 6436
Size: 275 186 20 27 1232 222 1069 1865
Cuts at: 6436 8207 10516 11149 11573 12091 12852 13880 1540
Size: 1771 2309 633 424 518 761 1028 2301
Fragments arranged by size:
2309 2301 1865 1771 1232 1069 1028 761
633 518 424 275 222 186 27 20
BsiHKAI G_wGCw'C
Cuts at: 3519 3747 4539 5472 5511 5913 5982 6186 6312
Size: 228 792 933 39 402 69 204 126
Cuts at: 6312 6862 8067 8275 8379 8562 9003 9516 9765
Size: 550 1205 208 104 183 441 513 249
Cuts at: 9765 9812 10897 11468 11597 12702 12787 13858 3519
Size: 47 1085 571 129 1105 85 1071 4302
Fragments arranged by size:
4302 1205 1105 1085 1071 933 792 571
550 513 441 402 249 228 208 204
183 129 126 104 85 69 47 39
BsiWI C'GTAC_G
Cuts at: 3161 3161
Size: 14641
BsmI GAATG_Cn'
Cuts at: 4057 4678 7825 11318 12152 12245 4057
Size: 621 3147 3493 834 93 6453
Fragments arranged by size:
6453 3493 3147 834 621 93
BsmBI CGTCTCn'nnnn_
Cuts at: 10492 11886 10492
Size: 1394 13247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2268 2307 2476 2498 2606 2944
Size: 399 331 147 39 169 22 108 338
Cuts at: 2944 3519 3747 4322 4516 4539 4588 5046 5139
Size: 575 228 575 194 23 49 458 93
Cuts at: 5139 5255 5472 5511 5913 5975 5982 6039 6153
Size: 116 217 39 402 62 7 57 114
Cuts at: 6153 6186 6312 6470 6512 6536 6862 6869 7013
Size: 33 126 158 42 24 326 7 144
Cuts at: 7013 7493 7866 8011 8055 8067 8087 8108 8255
Size: 480 373 145 44 12 20 21 147
Cuts at: 8255 8275 8294 8311 8379 8457 8473 8523 8562
Size: 20 19 17 68 78 16 50 39
Cuts at: 8562 8857 8867 9003 9025 9516 9765 9812 10047
Size: 295 10 136 22 491 249 47 235
Cuts at: 10047 10230 10507 10897 10913 11014 11083 11171 11346
Size: 183 277 390 16 101 69 88 175
Cuts at: 11346 11449 11468 11597 11813 11859 11960 12702 12787
Size: 103 19 129 216 46 101 742 85
Cuts at: 12787 13858 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 575 575 491 480 458
402 399 390 373 338 331 326 295
277 249 235 228 217 216 194 183
175 169 158 147 147 145 144 136
129 126 116 114 108 103 101 101
93 88 85 78 69 68 62 57
50 49 47 46 44 42 39 39
39 33 24 23 22 22 21 20
20 19 19 17 16 16 12 10
7 7
BspEI T'CCGG_A
Cuts at: 2197 2672 5145 14304 2197
Size: 475 2473 9159 2534
Fragments arranged by size:
9159 2534 2473 475
BspHI T'CATG_A
Cuts at: 3105 4519 9318 12530 14260 3105
Size: 1414 4799 3212 1730 3486
Fragments arranged by size:
4799 3486 3212 1730 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3213 3414 3652 4713 6479 7101 7925 8007
Size: 2369 201 238 1061 1766 622 824 82
Cuts at: 8007 8721 9886 11232 11996 844
Size: 714 1165 1346 764 3489
Fragments arranged by size:
3489 2369 1766 1346 1165 1061 824 764
714 622 238 201 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 3150 6651 10509 12528 1394
Size: 429 1294 33 3501 3858 2019 3507
Fragments arranged by size:
3858 3507 3501 2019 1294 429 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 5650 6806 10846 13136 13310 65
Size: 1183 4402 1156 4040 2290 174 1396
Fragments arranged by size:
4402 4040 2290 1396 1183 1156 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2554 2815 3065 3295 3882 4570 6431
Size: 110 744 261 250 230 587 688 1861
Cuts at: 6431 7542 7587 7992 8795 11043 11295 11631 13285
Size: 1111 45 405 803 2248 252 336 1654
Cuts at: 13285 13472 1700
Size: 187 2869
Fragments arranged by size:
2869 2248 1861 1654 1111 803 744 688
587 405 336 261 252 250 230 187
110 45
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 14641
BssHII G'CGCG_C
Cuts at: 1925 2609 3437 3439 6354 6554 1925
Size: 684 828 2 2915 200 10012
Fragments arranged by size:
10012 2915 828 684 200 2
BssSI C'ACGA_G
Cuts at: 6710 10040 11643 12701 13713 6710
Size: 3330 1603 1058 1012 7638
Fragments arranged by size:
7638 3330 1603 1058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4034 5782 6811 7555 8810 9877 10128 794
Size: 3240 1748 1029 744 1255 1067 251 5307
Fragments arranged by size:
5307 3240 1748 1255 1067 1029 744 251
BstBI TT'CG_AA
Cuts at: 13445 13445
Size: 14641
BstEII G'GTnAC_C
Cuts at: 1184 4777 1184
Size: 3593 11048
BstXI CCAn_nnnn'nTGG
Cuts at: 3256 3259 3739 5678 6760 7307 7651 8094 8626
Size: 3 480 1939 1082 547 344 443 532
Cuts at: 8626 8959 10639 10831 3256
Size: 333 1680 192 7066
Fragments arranged by size:
7066 1939 1680 1082 547 532 480 443
344 333 192 3
BstYI r'GATC_y
Cuts at: 1972 2723 3807 4950 5523 8405 9076 9216 9723
Size: 751 1084 1143 573 2882 671 140 507
Cuts at: 9723 10029 11971 12338 12723 12740 14192 14278 14290
Size: 306 1942 367 385 17 1452 86 12
Cuts at: 14290 14636 1972
Size: 346 1977
Fragments arranged by size:
2882 1977 1942 1452 1143 1084 751 671
573 507 385 367 346 306 140 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2482 7731 9250 9368 9999 10383 10556 2482
Size: 5249 1519 118 631 384 173 6567
Fragments arranged by size:
6567 5249 1519 631 384 173 118
BtgI C'CryG_G
Cuts at: 513 1065 2024 2542 3030 3226 3772 4042 4195
Size: 552 959 518 488 196 546 270 153
Cuts at: 4195 6741 6759 6918 7149 7306 7361 7644 8014
Size: 2546 18 159 231 157 55 283 370
Cuts at: 8014 8087 8207 8610 8952 9113 9352 9919 11369
Size: 73 120 403 342 161 239 567 1450
Cuts at: 11369 11403 11710 513
Size: 34 307 3444
Fragments arranged by size:
3444 2546 1450 959 567 552 546 518
488 403 370 342 307 283 270 239
231 196 161 159 157 153 120 73
55 34 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6235 487
Size: 683 56 5009 8893
Fragments arranged by size:
8893 5009 683 56
BtsI GCAGTG_nn'
Cuts at: 4525 5127 5271 5283 5783 7720 8087 8182 8183
Size: 602 144 12 500 1937 367 95 1
Cuts at: 8183 8221 8294 8691 8804 9455 9810 10507 11820
Size: 38 73 397 113 651 355 697 1313
Cuts at: 11820 12157 12951 12971 4525
Size: 337 794 20 6195
Fragments arranged by size:
6195 1937 1313 794 697 651 602 500
397 367 355 337 144 113 95 73
38 20 12 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 14641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3754 3789 7186 7221 9700 9735 529
Size: 35 3190 35 3397 35 2479 35 5435
Fragments arranged by size:
5435 3397 3190 2479 35 35 35 35
DraI TTT'AAA
Cuts at: 11945 11945
Size: 14641
DraIII CAC_nnn'GTG
Cuts at: 5767 7816 5767
Size: 2049 12592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5606 13648 809
Size: 507 4290 8042 1802
Fragments arranged by size:
8042 4290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 3246 3261
Size: 54 1425 50 275 18 188 1228 15
Cuts at: 3261 3499 3582 3708 3880 3884 3912 4568 4616
Size: 238 83 126 172 4 28 656 48
Cuts at: 4616 4984 5817 6433 6718 6915 7105 7540 7585
Size: 368 833 616 285 197 190 435 45
Cuts at: 7585 8314 8445 8653 9116 10513 10647 10753 10778
Size: 729 131 208 463 1397 134 106 25
Cuts at: 10778 10859 11297 11410 11570 12088 12977 14606 8
Size: 81 438 113 160 518 889 1629 43
Fragments arranged by size:
1629 1425 1397 1228 889 833 729 656
616 518 463 438 435 368 285 275
238 208 197 190 188 172 160 134
131 126 113 106 83 81 54 50
48 45 43 28 25 18 15 4
EagI C'GGCC_G
Cuts at: 1537 1812 2018 3499 4568 6433 10513 11570 12088
Size: 275 206 1481 1069 1865 4080 1057 518
Cuts at: 12088 1537
Size: 4090
Fragments arranged by size:
4090 4080 1865 1481 1069 1057 518 275
206
EarI CTCTTCn'nnn_
Cuts at: 1353 2852 5534 5627 6665 7020 7637 8534 9773
Size: 1499 2682 93 1038 355 617 897 1239
Cuts at: 9773 9869 10319 11209 12571 1353
Size: 96 450 890 1362 3423
Fragments arranged by size:
3423 2682 1499 1362 1239 1038 897 890
617 450 355 96 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2193 2449 2713 5465 6360 9485
Size: 1064 138 604 256 264 2752 895 3125
Cuts at: 9485 11466 13228 13602 13748 387
Size: 1981 1762 374 146 1280
Fragments arranged by size:
3125 2752 1981 1762 1280 1064 895 604
374 264 256 146 138
EcoICRI GAG'CTC
Cuts at: 6310 8560 9763 11595 6310
Size: 2250 1203 1832 9356
Fragments arranged by size:
9356 2250 1832 1203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2505 2931 3143 4715 5136 5388 7219
Size: 393 873 426 212 1572 421 252 1831
Cuts at: 7219 7583 7694 8537 8918 9150 9401 9620 9632
Size: 364 111 843 381 232 251 219 12
Cuts at: 9632 10075 10274 10598 10719 11057 11360 12704 13301
Size: 443 199 324 121 338 303 1344 597
Cuts at: 13301 14088 14356 1239
Size: 787 268 1524
Fragments arranged by size:
1831 1572 1524 1344 873 843 787 597
443 426 421 393 381 364 338 324
303 268 252 251 232 219 212 199
121 111 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2093 2775 5289 7634 2093
Size: 682 2514 2345 9100
Fragments arranged by size:
9100 2514 2345 682
EcoO109I rG'GnC_Cy
Cuts at: 2032 2131 2228 2303 2304 2441 2488 2715 3055
Size: 99 97 75 1 137 47 227 340
Cuts at: 3055 4753 5294 6035 6036 6532 6865 6866 7010
Size: 1698 541 741 1 496 333 1 144
Cuts at: 7010 7090 7190 7405 7669 7863 7891 8469 8470
Size: 80 100 215 264 194 28 578 1
Cuts at: 8470 8854 9021 9454 9525 9558 9568 9789 10294
Size: 384 167 433 71 33 10 221 505
Cuts at: 10294 10412 10419 11304 11494 11551 11810 11856 2032
Size: 118 7 885 190 57 259 46 4817
Fragments arranged by size:
4817 1698 885 741 578 541 505 496
433 384 340 333 264 259 227 221
215 194 190 167 144 137 118 100
99 97 80 75 71 57 47 46
33 28 10 7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 11950 12328 11950
Size: 378 14263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6709 7192 6709
Size: 483 14158
FseI GG_CCGG'CC
Cuts at: 3886 6435 3886
Size: 2549 12092
FspI TGC'GCA
Cuts at: 3860 9698 13147 3860
Size: 5838 3449 5354
Fragments arranged by size:
5838 5354 3449
HaeII r_GCGC'y
Cuts at: 1678 2289 2413 2822 2875 3082 3652 4116 5863
Size: 611 124 409 53 207 570 464 1747
Cuts at: 5863 6002 6030 6092 6525 6676 7225 8843 9927
Size: 139 28 62 433 151 549 1618 1084
Cuts at: 9927 10382 10479 10771 10924 11020 11403 13788 1678
Size: 455 97 292 153 96 383 2385 2531
Fragments arranged by size:
2531 2385 1747 1618 1084 611 570 549
464 455 433 409 383 292 207 153
151 139 124 97 96 62 53 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 3215 3247 3461
Size: 32 135 32 1060 32 551 32 214
Cuts at: 3461 3493 4139 4171 4184 4216 4435 4467 4715
Size: 32 646 32 13 32 219 32 248
Cuts at: 4715 4747 5078 5110 5348 5380 6997 7029 9409
Size: 32 331 32 238 32 1617 32 2380
Cuts at: 9409 9441 9781 9813 10667 10699 10835 10867 11570
Size: 32 340 32 854 32 136 32 703
Cuts at: 11570 11602 13282 13314 13356 13388 1373
Size: 32 1680 32 42 32 2626
Fragments arranged by size:
2626 2380 1680 1617 1060 854 703 646
551 340 331 248 238 219 214 136
135 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 5109 5485 5668 10240 11985
Size: 988 281 111 3060 376 183 4572 1745
Cuts at: 11985 12231 669
Size: 246 3079
Fragments arranged by size:
4572 3079 3060 1745 988 376 281 246
183 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 14641
HpaI GTT'AAC
Cuts at: 12231 12231
Size: 14641
KasI G'GCGC_C
Cuts at: 2409 2818 3078 6521 6672 9923 10475 10920 11399
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 11399 2409
Size: 5651
Fragments arranged by size:
5651 3443 3251 552 479 445 409 260
151
KpnI G_GTAC'C
Cuts at: 7369 11966 7369
Size: 4597 10044
MfeI C'AATT_G
Cuts at: 12240 12240
Size: 14641
MluI A'CGCG_T
Cuts at: 3040 14517 3040
Size: 11477 3164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3921 5571 5637 6259 6351 6777 10704 10798
Size: 2616 1650 66 622 92 426 3927 94
Cuts at: 10798 11712 13755 13939 1305
Size: 914 2043 184 2007
Fragments arranged by size:
3927 2616 2043 2007 1650 914 622 426
184 94 92 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3914 5819 6720 8447 8655
Size: 54 1425 343 2082 1905 901 1727 208
Cuts at: 8655 10649 10861 10
Size: 1994 212 3790
Fragments arranged by size:
3790 2082 1994 1905 1727 1425 901 343
212 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 3032 3254 3257 3605 3692 3923
Size: 648 1006 860 222 3 348 87 231
Cuts at: 3923 4325 4530 4634 4980 5276 5738 7101 7179
Size: 402 205 104 346 296 462 1363 78
Cuts at: 7179 7305 7349 7649 8058 8775 9087 9089 9519
Size: 126 44 300 409 717 312 2 430
Cuts at: 9519 9635 9732 10088 10662 12599 12958 13117 518
Size: 116 97 356 574 1937 359 159 2042
Fragments arranged by size:
2042 1937 1363 1006 860 717 648 574
462 430 409 402 359 356 348 346
312 300 296 231 222 205 159 126
116 104 97 87 78 44 3 2
MspA1I CmG'CkG
Cuts at: 1606 2569 2853 3032 3367 3434 3449 3498 3892
Size: 963 284 179 335 67 15 49 394
Cuts at: 3892 4313 5210 5656 5875 5924 6270 6438 7239
Size: 421 897 446 219 49 346 168 801
Cuts at: 7239 8209 8283 8331 8364 8635 8851 9802 9921
Size: 970 74 48 33 271 216 951 119
Cuts at: 9921 10540 10592 11135 11218 11536 11575 11614 11671
Size: 619 52 543 83 318 39 39 57
Cuts at: 11671 12057 12734 13882 14127 1606
Size: 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 963 951 897 801 677
619 543 446 421 394 386 346 335
318 284 271 245 219 216 179 168
119 83 74 67 57 52 49 49
48 39 39 33 15
NaeI GCC'GGC
Cuts at: 2817 3067 3884 6433 7589 7994 8797 11297 11633
Size: 250 817 2549 1156 405 803 2500 336
Cuts at: 11633 2817
Size: 5825
Fragments arranged by size:
5825 2549 2500 1156 817 803 405 336
250
NarI GG'CG_CC
Cuts at: 2410 2819 3079 6522 6673 9924 10476 10921 11400
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 11400 2410
Size: 5651
Fragments arranged by size:
5651 3443 3251 552 479 445 409 260
151
NcoI C'CATG_G
Cuts at: 513 1065 4042 4195 7149 7361 7644 8014 8610
Size: 552 2977 153 2954 212 283 370 596
Cuts at: 8610 8952 11403 11710 513
Size: 342 2451 307 3444
Fragments arranged by size:
3444 2977 2954 2451 596 552 370 342
307 283 212 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 14641
NgoMIV G'CCGG_C
Cuts at: 2815 3065 3882 6431 7587 7992 8795 11295 11631
Size: 250 817 2549 1156 405 803 2500 336
Cuts at: 11631 2815
Size: 5825
Fragments arranged by size:
5825 2549 2500 1156 817 803 405 336
250
NheI G'CTAG_C
Cuts at: 1052 6001 1052
Size: 4949 9692
NotI GC'GGCC_GC
Cuts at: 2018 12088 2018
Size: 10070 4571
NruI TCG'CGA
Cuts at: 3333 6635 3333
Size: 3302 11339
NsiI A_TGCA'T
Cuts at: 11318 11318
Size: 14641
NspI r_CATG'y
Cuts at: 1132 1229 2833 5851 9895 11999 13544 1132
Size: 97 1604 3018 4044 2104 1545 2229
Fragments arranged by size:
4044 3018 2229 2104 1604 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4423 8473 8506 8858 9748 9946 10867 1348
Size: 3075 4050 33 352 890 198 921 5122
Fragments arranged by size:
5122 4050 3075 921 890 352 198 33
PciI A'CATG_T
Cuts at: 5847 13540 5847
Size: 7693 6948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2356 3001 3817 3928 4122 5170 5323 8114
Size: 1248 645 816 111 194 1048 153 2791
Cuts at: 8114 8716 9212 9946 10297 10403 1108
Size: 602 496 734 351 106 5346
Fragments arranged by size:
5346 2791 1248 1048 816 734 645 602
496 351 194 153 111 106
PfoI T'CCnGG_A
Cuts at: 2214 4692 7635 9378 2214
Size: 2478 2943 1743 7477
Fragments arranged by size:
7477 2943 2478 1743
PmeI GTTT'AAAC
Cuts at: 11945 11945
Size: 14641
PmlI CAC'GTG
Cuts at: 6011 6378 7693 9637 6011
Size: 367 1315 1944 11015
Fragments arranged by size:
11015 1944 1315 367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3101 3133 12710 12742 14223 14255 3101
Size: 32 9577 32 1481 32 3487
Fragments arranged by size:
9577 3487 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 2441 2488 3055 7090 7405 7891 9454 9525
Size: 310 47 567 4035 315 486 1563 71
Cuts at: 9525 9558 9568 9789 10412 11304 11494 11551 2131
Size: 33 10 221 623 892 190 57 5221
Fragments arranged by size:
5221 4035 1563 892 623 567 486 315
310 221 190 71 57 47 33 10
PshAI GACnn'nnGTC
Cuts at: 1613 2392 5153 1613
Size: 779 2761 11101
Fragments arranged by size:
11101 2761 779
PsiI TTA'TAA
Cuts at: 12211 12211
Size: 14641
PspOMI G'GGCC_C
Cuts at: 2303 6035 6532 6865 7009 7862 8469 8853 9021
Size: 3732 497 333 144 853 607 384 168
Cuts at: 9021 11010 11809 11855 2303
Size: 1989 799 46 5089
Fragments arranged by size:
5089 3732 1989 853 799 607 497 384
333 168 144 46
PspXI vC'TCGA_Gb
Cuts at: 5891 5891
Size: 14641
PstI C_TGCA'G
Cuts at: 830 3366 3448 4950 5278 5655 6034 6063 6930
Size: 2536 82 1502 328 377 379 29 867
Cuts at: 6930 7668 9548 9679 9817 10902 11993 830
Size: 738 1880 131 138 1085 1091 3478
Fragments arranged by size:
3478 2536 1880 1502 1091 1085 867 738
379 377 328 138 131 82 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14641
PvuII CAG'CTG
Cuts at: 3367 3434 3449 4313 5210 5656 7239 8331 9802
Size: 67 15 864 897 446 1583 1092 1471
Cuts at: 9802 11135 11218 11614 11671 3367
Size: 1333 83 396 57 6337
Fragments arranged by size:
6337 1583 1471 1333 1092 897 864 446
396 83 67 57 15
SacI G_AGCT'C
Cuts at: 6312 8562 9765 11597 6312
Size: 2250 1203 1832 9356
Fragments arranged by size:
9356 2250 1832 1203
SacII CC_GC'GG
Cuts at: 3033 8210 9922 3033
Size: 5177 1712 7752
Fragments arranged by size:
7752 5177 1712
SalI G'TCGA_C
Cuts at: 1936 2047 11983 1936
Size: 111 9936 4594
Fragments arranged by size:
9936 4594 111
SanDI GG'GwC_CC
Cuts at: 2131 2488 9454 9525 11304 11551 2131
Size: 357 6966 71 1779 247 5221
Fragments arranged by size:
6966 5221 1779 357 247 71
SapI GCTCTTCn'nnn_
Cuts at: 6665 9869 10319 6665
Size: 3204 450 10987
Fragments arranged by size:
10987 3204 450
SbfI CC_TGCA'GG
Cuts at: 11993 11993
Size: 14641
ScaI AGT'ACT
Cuts at: 4067 12889 14601 4067
Size: 8822 1712 4107
Fragments arranged by size:
8822 4107 1712
SexAI A'CCwGG_T
Cuts at: 4773 7521 7579 11619 4773
Size: 2748 58 4040 7795
Fragments arranged by size:
7795 4040 2748 58
SfcI C'TryA_G
Cuts at: 826 1045 3362 3444 4946 5274 5651 6030 6059
Size: 219 2317 82 1502 328 377 379 29
Cuts at: 6059 6926 7664 9413 9544 9675 9813 10898 11989
Size: 867 738 1749 131 131 138 1085 1091
Cuts at: 11989 13124 13805 13996 826
Size: 1135 681 191 1471
Fragments arranged by size:
2317 1749 1502 1471 1135 1091 1085 867
738 681 379 377 328 219 191 138
131 131 82 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 7593 10293 7593
Size: 2700 11941
SfoI GGC'GCC
Cuts at: 2411 2820 3080 6523 6674 9925 10477 10922 11401
Size: 409 260 3443 151 3251 552 445 479
Cuts at: 11401 2411
Size: 5651
Fragments arranged by size:
5651 3443 3251 552 479 445 409 260
151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14641
SgrAI Cr'CCGG_yG
Cuts at: 1700 3065 1700
Size: 1365 13276
SmaI CCC'GGG
Cuts at: 1872 2493 2840 2878 2892 3571 8465 9017 11492
Size: 621 347 38 14 679 4894 552 2475
Cuts at: 11492 11968 1872
Size: 476 4545
Fragments arranged by size:
4894 4545 2475 679 621 552 476 347
38 14
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4188 5840 5891 6787 9172 9704
Size: 197 926 2245 1652 51 896 2385 532
Cuts at: 9704 11451 11545 12745 13646 13908 820
Size: 1747 94 1200 901 262 1553
Fragments arranged by size:
2385 2245 1747 1652 1553 1200 926 901
896 532 262 197 94 51
SnaBI TAC'GTA
Cuts at: 493 12432 493
Size: 11939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 14641
SphI G_CATG'C
Cuts at: 1132 2833 9895 11999 1132
Size: 1701 7062 2104 3774
Fragments arranged by size:
7062 3774 2104 1701
SspI AAT'ATT
Cuts at: 5 52 12565 5
Size: 47 12513 2081
Fragments arranged by size:
12513 2081 47
StuI AGG'CCT
Cuts at: 1517 7701 1517
Size: 6184 8457
StyI C'CwwG_G
Cuts at: 513 1065 2159 3999 4042 4195 6850 6873 7149
Size: 552 1094 1840 43 153 2655 23 276
Cuts at: 7149 7361 7644 8014 8311 8610 8952 10701 11112
Size: 212 283 370 297 299 342 1749 411
Cuts at: 11112 11403 11653 11710 11805 12080 513
Size: 291 250 57 95 275 3074
Fragments arranged by size:
3074 2655 1840 1749 1094 552 411 370
342 299 297 291 283 276 275 250
212 153 95 57 43 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4436 4655 6320 8027 8465 9101 9647
Size: 1255 2856 219 1665 1707 438 636 546
Cuts at: 9647 10832 11683 12668 12685 12838 13023 13468 325
Size: 1185 851 985 17 153 185 445 1498
Fragments arranged by size:
2856 1707 1665 1498 1255 1185 985 851
636 546 445 438 219 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 4065 4995
Size: 274 80 33 51 1443 1008 1080 930
Cuts at: 4995 12887 14599 96
Size: 7892 1712 138
Fragments arranged by size:
7892 1712 1443 1080 1008 930 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5187 5219 5428 5460 8066 8098 11458 11490 14601
Size: 32 209 32 2606 32 3360 32 3111
Cuts at: 14601 14633 5187
Size: 32 5195
Fragments arranged by size:
5195 3360 3111 2606 209 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 10565 11693 1316
Size: 9249 1128 4264
Fragments arranged by size:
9249 4264 1128
XbaI T'CTAG_A
Cuts at: 11207 11977 11207
Size: 770 13871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 3256 3502 3922 6251 6880 6994 7304 8094
Size: 683 246 420 2329 629 114 310 790
Cuts at: 8094 8432 9175 10622 10664 11728 2573
Size: 338 743 1447 42 1064 5486
Fragments arranged by size:
5486 2329 1447 1064 790 743 683 629
420 338 310 246 114 42
XhoI C'TCGA_G
Cuts at: 1943 4188 5891 11451 1943
Size: 2245 1703 5560 5133
Fragments arranged by size:
5560 5133 2245 1703
XmaI C'CCGG_G
Cuts at: 1870 2491 2838 2876 2890 3569 8463 9015 11490
Size: 621 347 38 14 679 4894 552 2475
Cuts at: 11490 11966 1870
Size: 476 4545
Fragments arranged by size:
4894 4545 2475 679 621 552 476 347
38 14
XmnI GAAnn'nnTTC
Cuts at: 12770 12770
Size: 14641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5932 12449 276
Size: 53 83 186 936 4398 6517 2468
Fragments arranged by size:
6517 4398 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BstZ17I EcoRV FspAI PacI PsrI RsrII
SrfI SwaI