Restriction map for
pFN21AE2303
(Circular) (Six-base) MAPSORT of: pFN21AE2303.seq Check: 8395 from: 1 to: 11335
pFN21AE2303 11335 bp
With 182 enzymes: SgfI *
November 26, 2010 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2623 4492 2623
Size: 1869 9466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9145 278
Size: 53 83 186 936 7609 2468
Fragments arranged by size:
7609 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3782 6336 8678 1937
Size: 1845 2554 2342 4594
Fragments arranged by size:
4594 2554 2342 1845
Acc65I G'GTAC_C
Cuts at: 4259 8656 4259
Size: 4397 6938
AclI AA'CG_TT
Cuts at: 1563 3301 9462 9835 1563
Size: 1738 6161 373 3063
Fragments arranged by size:
6161 3063 1738 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2832 4883 4916 6301 6801 9398 10782 11050
Size: 1200 2051 33 1385 500 2597 1384 268
Cuts at: 11050 1632
Size: 1917
Fragments arranged by size:
2597 2051 1917 1385 1384 1200 500 268
33
AfeI AGC'GCT
Cuts at: 5083 7514 5083
Size: 2431 8904
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11138
AflIII A'CryG_T
Cuts at: 1203 10234 11211 1203
Size: 9031 977 1327
Fragments arranged by size:
9031 1327 977
AgeI A'CCGG_T
Cuts at: 6760 10166 6760
Size: 3406 7929
AhdI GACnn_n'nnGTC
Cuts at: 10064 10064
Size: 11335
AleI CACnn'nnGTG
Cuts at: 1166 2543 4295 5066 1166
Size: 1377 1752 771 7435
Fragments arranged by size:
7435 1752 1377 771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3054 4025 4380 1184
Size: 1870 971 355 8139
Fragments arranged by size:
8139 1870 971 355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2991 3023 8132 8164 2991
Size: 32 5109 32 6162
Fragments arranged by size:
6162 5109 32 32
AlwNI CAG_nnn'CTG
Cuts at: 6042 6427 7898 8287 9078 10650 6042
Size: 385 1471 389 791 1572 6727
Fragments arranged by size:
6727 1572 1471 791 389 385
ApaI G_GGCC'C
Cuts at: 3318 6645 3318
Size: 3327 8008
ApaLI G'TGCA_C
Cuts at: 4808 5915 9392 10548 4808
Size: 1107 3477 1156 5595
Fragments arranged by size:
5595 3477 1156 1107
ApoI r'AATT_y
Cuts at: 1493 4739 5572 5725 6984 7162 8449 8644 8870
Size: 3246 833 153 1259 178 1287 195 226
Cuts at: 8870 9022 11131 1493
Size: 152 2109 1697
Fragments arranged by size:
3246 2109 1697 1287 1259 833 226 195
178 153 152
AseI AT'TA_AT
Cuts at: 160 1032 8129 9889 160
Size: 872 7097 1760 1606
Fragments arranged by size:
7097 1760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11335
AvaI C'yCGr_G
Cuts at: 1870 1943 4781 8660 1870
Size: 73 2838 3879 4545
Fragments arranged by size:
4545 3879 2838 73
AvrII C'CTAG_G
Cuts at: 3010 3010
Size: 11335
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5467 5500 5467
Size: 33 11302
BamHI G'GATC_C
Cuts at: 3250 4841 8665 9032 3250
Size: 1591 3824 367 5553
Fragments arranged by size:
5553 3824 1591 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2400 4259 4297 4414 8656
Size: 325 217 627 613 1859 38 117 4242
Cuts at: 8656 10111 618
Size: 1455 1842
Fragments arranged by size:
4242 1859 1842 1455 627 613 325 217
117 38
BanII G_rGCy'C
Cuts at: 1391 3318 3957 4706 5295 6645 6929 7869 7913
Size: 1927 639 749 589 1350 284 940 44
Cuts at: 7913 8210 8654 1391
Size: 297 444 4072
Fragments arranged by size:
4072 1927 1350 940 749 639 589 444
297 284 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2601 2778 4530 8237 928
Size: 971 702 177 1752 3707 4026
Fragments arranged by size:
4026 3707 1752 971 702 177
BbvCI CC'TCA_GC
Cuts at: 3687 3687
Size: 11335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3893 3927 9526 9560 1903
Size: 34 1956 34 5599 34 3678
Fragments arranged by size:
5599 3678 1956 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7754 9227 10437 7754
Size: 1473 1210 8652
Fragments arranged by size:
8652 1473 1210
BclI T'GATC_A
Cuts at: 1548 2577 1548
Size: 1029 10306
BfrBI ATG'CAT
Cuts at: 3156 3498 3156
Size: 342 10993
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9946 136
Size: 107 122 71 631 605 8274 1525
Fragments arranged by size:
8274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 11330 11330
Size: 11335
BlpI GC'TnA_GC
Cuts at: 8752 8752
Size: 11335
Bme1580I G_kGCm'C
Cuts at: 1790 3318 4812 5198 5919 6645 8378 9396 10552
Size: 1528 1494 386 721 726 1733 1018 1156
Cuts at: 10552 1790
Size: 2573
Fragments arranged by size:
2573 1733 1528 1494 1156 1018 726 721
386
BmgBI CAC'GTC
Cuts at: 1318 8490 1318
Size: 7172 4163
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 3166 3918
Size: 416 53 83 399 24 615 1134 752
Cuts at: 3918 4063 6867 8145 8162 8329 9066 10024 442
Size: 145 2804 1278 17 167 737 958 1753
Fragments arranged by size:
2804 1753 1278 1134 958 752 737 615
416 399 167 145 83 53 24 17
BmtI G_CTAG'C
Cuts at: 1056 3225 1056
Size: 2169 9166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4009 6948 7018 7096 4009
Size: 2939 70 78 8248
Fragments arranged by size:
8248 2939 78 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2928 2979 3755 3933 3941 4140 4369
Size: 827 862 51 776 178 8 199 229
Cuts at: 4369 5109 5675 5800 7011 7160 7705 8013 9995
Size: 740 566 125 1211 149 545 308 1982
Cuts at: 9995 1239
Size: 2579
Fragments arranged by size:
2579 1982 1211 862 827 776 740 566
545 308 229 199 178 149 125 51
8
Bpu10I CC'TnA_GC
Cuts at: 3687 6135 6646 7149 7365 3687
Size: 2448 511 503 216 7657
Fragments arranged by size:
7657 2448 511 503 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6346 7340 7772 7953 9460 10325 10623 6346
Size: 994 432 181 1507 865 298 7058
Fragments arranged by size:
7058 1507 994 865 432 298 181
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3442 6769 7553 9998 882
Size: 617 1943 3327 784 2445 2219
Fragments arranged by size:
3327 2445 2219 1943 784 617
BsaAI yAC'GTr
Cuts at: 493 1204 6386 7062 7619 8544 9126 493
Size: 711 5182 676 557 925 582 2702
Fragments arranged by size:
5182 2702 925 711 676 582 557
BsaBI GATnn'nnATC
Cuts at: 5366 11329 5366
Size: 5963 5372
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2221 7711 9142 9524
Size: 53 83 186 936 688 5490 1431 382
Cuts at: 9524 10159 275
Size: 635 1451
Fragments arranged by size:
5490 1451 1431 936 688 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6760 9768 10166 10440 10587 10998 6760
Size: 3008 398 274 147 411 7097
Fragments arranged by size:
7097 3008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3753 3783 8221 8251 1178
Size: 30 165 30 2350 30 4438 30 4262
Fragments arranged by size:
4438 4262 2350 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 3196 3538 5672 5675 5678 5950 6491
Size: 1414 596 342 2134 3 3 272 541
Cuts at: 6491 7088 7615 8282 1186
Size: 597 527 667 4239
Fragments arranged by size:
4239 2134 1414 667 597 596 541 527
342 272 3 3
BseYI C'CCAG_C
Cuts at: 2152 2556 2692 5089 6638 6673 6851 7373 7438
Size: 404 136 2397 1549 35 178 522 65
Cuts at: 7438 10538 11034 2152
Size: 3100 496 2453
Fragments arranged by size:
3100 2453 2397 1549 522 496 404 178
136 65 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 3327 3375 6083 7297 7516 7599
Size: 573 865 726 48 2708 1214 219 83
Cuts at: 7599 1163
Size: 4899
Fragments arranged by size:
4899 2708 1214 865 726 573 219 83
48
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8785 9546 10574 1540
Size: 275 186 6784 761 1028 2301
Fragments arranged by size:
6784 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3957 4004 4812 5919 6929 7482 8601 9396 9481
Size: 47 808 1107 1010 553 1119 795 85
Cuts at: 9481 10552 3957
Size: 1071 4740
Fragments arranged by size:
4740 1119 1107 1071 1010 808 795 553
85 47
BsmI GAATG_Cn'
Cuts at: 3158 5831 6640 8846 8939 3158
Size: 2673 809 2206 93 5554
Fragments arranged by size:
5554 2673 2206 809 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3920 7439 3920
Size: 3519 7816
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3318 3957 4004 4706 4812 5198 5295
Size: 399 1528 639 47 702 106 386 97
Cuts at: 5295 5919 6645 6929 7482 7869 7913 8210 8378
Size: 624 726 284 553 387 44 297 168
Cuts at: 8378 8601 8654 9396 9481 10552 1391
Size: 223 53 742 85 1071 2174
Fragments arranged by size:
2174 1528 1071 742 726 702 639 624
553 399 387 386 297 284 223 168
106 97 85 53 47 44
BspEI T'CCGG_A
Cuts at: 10998 10998
Size: 11335
BspHI T'CATG_A
Cuts at: 2302 8622 9224 10954 2302
Size: 6320 602 1730 2683
Fragments arranged by size:
6320 2683 1730 602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 2302 2623 4492 7571 8690 844
Size: 1370 88 321 1869 3079 1119 3489
Fragments arranged by size:
3489 3079 1869 1370 1119 321 88
BsrBI CCG'CTC
Cuts at: 1394 1823 6471 7369 9222 1394
Size: 429 4648 898 1853 3507
Fragments arranged by size:
4648 3507 1853 898 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2916 4007 4097 5941 9830 10004 65
Size: 1183 1668 1091 90 1844 3889 174 1396
Fragments arranged by size:
3889 1844 1668 1396 1183 1091 174 90
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 6760 9979 10166 1700
Size: 110 2484 2466 3219 187 2869
Fragments arranged by size:
3219 2869 2484 2466 187 110
BsrGI T'GTAC_A
Cuts at: 96 7982 8610 96
Size: 7886 628 2821
Fragments arranged by size:
7886 2821 628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11335
BssSI C'ACGA_G
Cuts at: 3693 9395 10407 3693
Size: 5702 1012 4621
Fragments arranged by size:
5702 4621 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 11335
BstBI TT'CG_AA
Cuts at: 10139 10139
Size: 11335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11335
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 3049 6078 6160 8023 2287
Size: 762 3029 82 1863 5599
Fragments arranged by size:
5599 3029 1863 762 82
BstYI r'GATC_y
Cuts at: 1972 3250 4841 5029 7299 8665 9032 9417 9434
Size: 1278 1591 188 2270 1366 367 385 17
Cuts at: 9434 10886 10972 10984 11330 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2270 1977 1591 1452 1366 1278 385 367
346 188 86 17 12
BstZ17I GTA'TAC
Cuts at: 6337 6337
Size: 11335
Bsu36I CC'TnA_GG
Cuts at: 5634 5634
Size: 11335
BtgI C'CryG_G
Cuts at: 513 1065 6990 7251 7543 7720 7860 513
Size: 552 5925 261 292 177 140 3988
Fragments arranged by size:
5925 3988 552 292 261 177 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10596
Fragments arranged by size:
10596 683 56
BtsI GCAGTG_nn'
Cuts at: 4002 5196 6688 7286 8851 9645 9665 4002
Size: 1194 1492 598 1565 794 20 5672
Fragments arranged by size:
5672 1565 1492 1194 794 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4596 4631 529
Size: 35 4032 35 7233
Fragments arranged by size:
7233 4032 35 35
DraI TTT'AAA
Cuts at: 3297 4045 5374 8639 3297
Size: 748 1329 3265 5993
Fragments arranged by size:
5993 3265 1329 748
DraIII CAC_nnn'GTG
Cuts at: 3926 8593 3926
Size: 4667 6668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3800 7710 10342 809
Size: 507 2484 3910 2632 1802
Fragments arranged by size:
3910 2632 2484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3483 3591 7717
Size: 54 1425 50 275 18 1653 108 4126
Cuts at: 7717 7921 8553 8782 9671 11300 8
Size: 204 632 229 889 1629 43
Fragments arranged by size:
4126 1653 1629 1425 889 632 275 229
204 108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8782 1537
Size: 275 6970 4090
Fragments arranged by size:
6970 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 3207 4543 5543 5769 6817 6934 7487
Size: 1022 832 1336 1000 226 1048 117 553
Cuts at: 7487 8167 8387 9265 1353
Size: 680 220 878 3423
Fragments arranged by size:
3423 1336 1048 1022 1000 878 832 680
553 226 220 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5447 9922 10296 10442 387
Size: 1064 138 3858 4475 374 146 1280
Fragments arranged by size:
4475 3858 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3955 6927 3955
Size: 2972 8363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2832 2928 2979 3755 3933 3941
Size: 393 434 766 96 51 776 178 8
Cuts at: 3941 4140 4369 4883 4916 5109 5675 5800 6301
Size: 199 229 514 33 193 566 125 501
Cuts at: 6301 6801 7011 7160 7705 8013 9398 9995 10782
Size: 500 210 149 545 308 1385 597 787
Cuts at: 10782 11050 1239
Size: 268 1524
Fragments arranged by size:
1524 1385 787 776 766 597 566 545
514 501 500 434 393 308 268 229
210 199 193 178 149 125 96 51
33 8
EcoNI CCTnn'n_nnAGG
Cuts at: 4397 5633 4397
Size: 1236 10099
EcoO109I rG'GnC_Cy
Cuts at: 2953 3474 3870 5169 6039 6642 7875 2953
Size: 521 396 1299 870 603 1233 6413
Fragments arranged by size:
6413 1299 1233 870 603 521 396
EcoRI G'AATT_C
Cuts at: 4739 5572 6984 8449 8644 9022 4739
Size: 833 1412 1465 195 378 7052
Fragments arranged by size:
7052 1465 1412 833 378 195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 11335
FspI TGC'GCA
Cuts at: 6513 9841 6513
Size: 3328 8007
HaeII r_GCGC'y
Cuts at: 1678 5085 5605 7516 10482 1678
Size: 3407 520 1911 2966 2531
Fragments arranged by size:
3407 2966 2531 1911 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2371 2403 2477
Size: 32 135 32 470 32 297 32 74
Cuts at: 2477 2509 3070 3102 3710 3742 3753 3785 3810
Size: 32 561 32 608 32 11 32 25
Cuts at: 3810 3842 5228 5260 7680 7712 9976 10008 10050
Size: 32 1386 32 2420 32 2264 32 42
Cuts at: 10050 10082 1373
Size: 32 2626
Fragments arranged by size:
2626 2420 2264 1386 608 561 470 297
135 74 42 32 32 32 32 32
32 32 32 32 32 32 32 32
25 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2476 3195 8086 8679 8925 669
Size: 988 281 538 719 4891 593 246 3079
Fragments arranged by size:
4891 3079 988 719 593 538 281 246
HindIII A'AGCT_T
Cuts at: 748 3290 748
Size: 2542 8793
HpaI GTT'AAC
Cuts at: 8925 8925
Size: 11335
KpnI G_GTAC'C
Cuts at: 4263 8660 4263
Size: 4397 6938
MfeI C'AATT_G
Cuts at: 8934 8934
Size: 11335
MluI A'CGCG_T
Cuts at: 11211 11211
Size: 11335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2470 3224 3278 5535 5820 6210 6817
Size: 780 385 754 54 2257 285 390 607
Cuts at: 6817 8207 8472 10449 10633 1305
Size: 1390 265 1977 184 2007
Fragments arranged by size:
2257 2007 1977 1390 780 754 607 390
385 285 265 184 54
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7719 10
Size: 54 1425 343 5887 3626
Fragments arranged by size:
5887 3626 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 4295 5066 5934 5951 6252 6401
Size: 648 1377 1752 771 868 17 301 149
Cuts at: 6401 7043 7130 7346 7994 8021 8129 9293 9652
Size: 642 87 216 648 27 108 1164 359
Cuts at: 9652 9811 518
Size: 159 2042
Fragments arranged by size:
2042 1752 1377 1164 868 771 648 648
642 359 301 216 159 149 108 87
27 17
MspA1I CmG'CkG
Cuts at: 1606 2190 3270 5394 5481 6677 7031 8458 8751
Size: 584 1080 2124 87 1196 354 1427 293
Cuts at: 8751 9428 10576 10821 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2124 2120 1427 1196 1148 1080 677 584
354 293 245 87
NcoI C'CATG_G
Cuts at: 513 1065 7251 7543 7720 7860 513
Size: 552 6186 292 177 140 3988
Fragments arranged by size:
6186 3988 552 292 177 140
NdeI CA'TA_TG
Cuts at: 387 3129 4804 6238 7155 387
Size: 2742 1675 1434 917 4567
Fragments arranged by size:
4567 2742 1675 1434 917
NheI G'CTAG_C
Cuts at: 1052 3221 1052
Size: 2169 9166
NotI GC'GGCC_GC
Cuts at: 8782 8782
Size: 11335
NsiI A_TGCA'T
Cuts at: 3158 3500 3158
Size: 342 10993
NspI r_CATG'y
Cuts at: 1132 1229 5956 6062 7201 7945 8693 10238 1132
Size: 97 4727 106 1139 744 748 1545 2229
Fragments arranged by size:
4727 2229 1545 1139 748 744 106 97
PasI CC'CwG_GG
Cuts at: 1348 6917 7870 1348
Size: 5569 953 4813
Fragments arranged by size:
5569 4813 953
PciI A'CATG_T
Cuts at: 10234 10234
Size: 11335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5971 6097 8593 1108
Size: 4863 126 2496 3850
Fragments arranged by size:
4863 3850 2496 126
PfoI T'CCnGG_A
Cuts at: 5730 5730
Size: 11335
PmeI GTTT'AAAC
Cuts at: 8639 8639
Size: 11335
PmlI CAC'GTG
Cuts at: 8544 8544
Size: 11335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4542 4574 9404 9436 10917 10949 4542
Size: 32 4830 32 1481 32 4928
Fragments arranged by size:
4928 4830 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2953 5169 6039 7875 2953
Size: 2216 870 1836 6413
Fragments arranged by size:
6413 2216 1836 870
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11335
PsiI TTA'TAA
Cuts at: 4653 8905 4653
Size: 4252 7083
PspOMI G'GGCC_C
Cuts at: 3314 6641 3314
Size: 3327 8008
PstI C_TGCA'G
Cuts at: 830 2313 4513 4975 5596 5997 6558 6727 7009
Size: 1483 2200 462 621 401 561 169 282
Cuts at: 7009 7072 7206 7336 8687 830
Size: 63 134 130 1351 3478
Fragments arranged by size:
3478 2200 1483 1351 621 561 462 401
282 169 134 130 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11335
PvuII CAG'CTG
Cuts at: 3270 5394 6677 7031 3270
Size: 2124 1283 354 7574
Fragments arranged by size:
7574 2124 1283 354
SacI G_AGCT'C
Cuts at: 3957 6929 3957
Size: 2972 8363
SalI G'TCGA_C
Cuts at: 1936 8677 1936
Size: 6741 4594
SapI GCTCTTCn'nnn_
Cuts at: 2375 3207 4543 6934 7487 2375
Size: 832 1336 2391 553 6223
Fragments arranged by size:
6223 2391 1336 832 553
SbfI CC_TGCA'GG
Cuts at: 8687 8687
Size: 11335
ScaI AGT'ACT
Cuts at: 2631 9583 11295 2631
Size: 6952 1712 2671
Fragments arranged by size:
6952 2671 1712
SexAI A'CCwGG_T
Cuts at: 3048 4279 3048
Size: 1231 10104
SfcI C'TryA_G
Cuts at: 826 1045 2309 4509 4971 5592 5993 6554 6723
Size: 219 1264 2200 462 621 401 561 169
Cuts at: 6723 7005 7068 7202 7332 7631 7766 8683 9818
Size: 282 63 134 130 299 135 917 1135
Cuts at: 9818 10499 10690 826
Size: 681 191 1471
Fragments arranged by size:
2200 1471 1264 1135 917 681 621 561
462 401 299 282 219 191 169 135
134 130 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11335
SgrAI Cr'CCGG_yG
Cuts at: 1700 4294 1700
Size: 2594 8741
SmaI CCC'GGG
Cuts at: 1872 8662 1872
Size: 6790 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6325 7355 7787 7968 9439 10340
Size: 197 926 4382 1030 432 181 1471 901
Cuts at: 10340 10602 820
Size: 262 1553
Fragments arranged by size:
4382 1553 1471 1030 926 901 432 262
197 181
SnaBI TAC'GTA
Cuts at: 493 9126 493
Size: 8633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11335
SphI G_CATG'C
Cuts at: 1132 5956 8693 1132
Size: 4824 2737 3774
Fragments arranged by size:
4824 3774 2737
SspI AAT'ATT
Cuts at: 5 52 7576 9259 5
Size: 47 7524 1683 2081
Fragments arranged by size:
7524 2081 1683 47
StuI AGG'CCT
Cuts at: 1517 3946 6290 1517
Size: 2429 2344 6562
Fragments arranged by size:
6562 2429 2344
StyI C'CwwG_G
Cuts at: 513 1065 3010 4263 6464 7251 7543 7720 7750
Size: 552 1945 1253 2201 787 292 177 30
Cuts at: 7750 7860 8293 8774 513
Size: 110 433 481 3074
Fragments arranged by size:
3074 2201 1945 1253 787 552 481 433
292 177 110 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2014 2279 3718 3937 4542 5683 8573
Size: 1255 434 265 1439 219 605 1141 2890
Cuts at: 8573 9362 9379 9532 9717 10162 325
Size: 789 17 153 185 445 1498
Fragments arranged by size:
2890 1498 1439 1255 1141 789 605 445
434 265 219 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2629 7194 7266
Size: 274 80 33 51 1443 652 4565 72
Cuts at: 7266 7982 8520 8610 9581 11293 96
Size: 716 538 90 971 1712 138
Fragments arranged by size:
4565 1712 1443 971 716 652 538 274
138 90 80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3714 3746 4071 4103 5614 5646 5712
Size: 32 644 32 325 32 1511 32 66
Cuts at: 5712 5744 6399 6431 7295 7327 8187 8219 8221
Size: 32 655 32 864 32 860 32 2
Cuts at: 8221 8253 8533 8565 11295 11327 3038
Size: 32 280 32 2730 32 3046
Fragments arranged by size:
3046 2730 1511 864 860 655 644 325
280 66 32 32 32 32 32 32
32 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11335
XbaI T'CTAG_A
Cuts at: 8252 8671 8252
Size: 419 10916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2921 2921
Size: 11335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11335
XmaI C'CCGG_G
Cuts at: 1870 8660 1870
Size: 6790 4545
XmnI GAAnn'nnTTC
Cuts at: 9464 9464
Size: 11335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9143 276
Size: 53 83 186 936 7609 2468
Fragments arranged by size:
7609 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BsiWI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI