Restriction map for
pFN21AE2300
(Circular) (Six-base) MAPSORT of: pFN21AE2300.seq Check: 4938 from: 1 to: 7276
pFN21AE2300 7276 bp
With 182 enzymes: SgfI *
April 6, 2011 13:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3442 3442
Size: 7276
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5086 278
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4619 1937
Size: 2682 4594
Acc65I G'GTAC_C
Cuts at: 4597 4597
Size: 7276
AclI AA'CG_TT
Cuts at: 1563 5403 5776 1563
Size: 3840 373 3063
Fragments arranged by size:
3840 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3092 3525 4443 5339 6723 6991 1632
Size: 1460 433 918 896 1384 268 1917
Fragments arranged by size:
1917 1460 1384 918 896 433 268
AfeI AGC'GCT
Cuts at: 2295 2295
Size: 7276
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7079
AflIII A'CryG_T
Cuts at: 1203 6175 7152 1203
Size: 4972 977 1327
Fragments arranged by size:
4972 1327 977
AgeI A'CCGG_T
Cuts at: 6107 6107
Size: 7276
AhdI GACnn_n'nnGTC
Cuts at: 6005 6005
Size: 7276
AleI CACnn'nnGTG
Cuts at: 1166 4512 1166
Size: 3346 3930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3568 1184
Size: 2384 4892
AlwNI CAG_nnn'CTG
Cuts at: 5019 6591 5019
Size: 1572 5704
ApaLI G'TGCA_C
Cuts at: 5333 6489 5333
Size: 1156 6120
ApoI r'AATT_y
Cuts at: 1493 2124 2337 2426 2560 2608 2773 2980 3517
Size: 631 213 89 134 48 165 207 537
Cuts at: 3517 3573 4268 4380 4462 4585 4811 4963 7072
Size: 56 695 112 82 123 226 152 2109
Cuts at: 7072 1493
Size: 1697
Fragments arranged by size:
2109 1697 695 631 537 226 213 207
165 152 134 123 112 89 82 56
48
AseI AT'TA_AT
Cuts at: 160 1032 4218 5830 160
Size: 872 3186 1612 1606
Fragments arranged by size:
3186 1612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 4601 1870
Size: 73 206 2452 4545
Fragments arranged by size:
4545 2452 206 73
BamHI G'GATC_C
Cuts at: 4606 4973 4606
Size: 367 6909
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4597 6052 618
Size: 325 217 627 2810 1455 1842
Fragments arranged by size:
2810 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4359 4595 1391
Size: 2968 236 4072
Fragments arranged by size:
4072 2968 236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 2870 3086 4092 928
Size: 971 201 770 216 1006 4112
Fragments arranged by size:
4112 1006 971 770 216 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5467 5501 1903
Size: 34 3530 34 3678
Fragments arranged by size:
3678 3530 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5168 6378 5168
Size: 1210 6066
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7276
BfrBI ATG'CAT
Cuts at: 3555 3555
Size: 7276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5887 136
Size: 107 122 71 631 605 4215 1525
Fragments arranged by size:
4215 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4291 7271 4291
Size: 2980 4296
BlpI GC'TnA_GC
Cuts at: 4693 4693
Size: 7276
Bme1580I G_kGCm'C
Cuts at: 1790 5337 6493 1790
Size: 3547 1156 2573
Fragments arranged by size:
3547 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7276
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2822 5007 5965
Size: 416 53 83 399 24 1405 2185 958
Cuts at: 5965 442
Size: 1753
Fragments arranged by size:
2185 1753 1405 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3393 4090 5936 1239
Size: 2154 697 1846 2579
Fragments arranged by size:
2579 2154 1846 697
Bpu10I CC'TnA_GC
Cuts at: 4353 4353
Size: 7276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5401 6266 6564 5401
Size: 865 298 6113
Fragments arranged by size:
6113 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5939 882
Size: 617 4440 2219
Fragments arranged by size:
4440 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2390 5067 493
Size: 711 1186 2677 2702
Fragments arranged by size:
2702 2677 1186 711
BsaBI GATnn'nnATC
Cuts at: 4254 7270 4254
Size: 3016 4260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5083 5465 6100 275
Size: 53 83 186 936 3550 382 635 1451
Fragments arranged by size:
3550 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2651 2769 3232 3708 5709 6107 6381 6528 6939
Size: 118 463 476 2001 398 274 147 411
Cuts at: 6939 2651
Size: 2988
Fragments arranged by size:
2988 2001 476 463 411 398 274 147
118
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7051
Fragments arranged by size:
7051 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 1186
Size: 1423 5853
BseYI C'CCAG_C
Cuts at: 6479 6975 6479
Size: 496 6780
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6703
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4726 5487 6515 1540
Size: 275 186 2725 761 1028 2301
Fragments arranged by size:
2725 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4359 5337 5422 6493 4359
Size: 978 85 1071 5142
Fragments arranged by size:
5142 1071 978 85
BsmI GAATG_Cn'
Cuts at: 2924 3557 3984 4787 4880 2924
Size: 633 427 803 93 5320
Fragments arranged by size:
5320 803 633 427 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4359 4595 5337 5422 6493 1391
Size: 399 2569 236 742 85 1071 2174
Fragments arranged by size:
2569 2174 1071 742 399 236 85
BspEI T'CCGG_A
Cuts at: 3232 6939 3232
Size: 3707 3569
BspHI T'CATG_A
Cuts at: 4258 5165 6895 4258
Size: 907 1730 4639
Fragments arranged by size:
4639 1730 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3442 3475 4631 844
Size: 2598 33 1156 3489
Fragments arranged by size:
3489 2598 1156 33
BsrBI CCG'CTC
Cuts at: 1394 1823 5163 1394
Size: 429 3340 3507
Fragments arranged by size:
3507 3340 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5771 5945 65
Size: 1183 4523 174 1396
Fragments arranged by size:
4523 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5920 6107 1700
Size: 110 4110 187 2869
Fragments arranged by size:
4110 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7276
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7276
BssSI C'ACGA_G
Cuts at: 5336 6348 5336
Size: 1012 6264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7276
BstBI TT'CG_AA
Cuts at: 6080 6080
Size: 7276
BstEII G'GTnAC_C
Cuts at: 1184 2765 1184
Size: 1581 5695
BstXI CCAn_nnnn'nTGG
Cuts at: 2196 3926 4454 2196
Size: 1730 528 5018
Fragments arranged by size:
5018 1730 528
BstYI r'GATC_y
Cuts at: 1972 2942 4291 4606 4973 5358 5375 6827 6913
Size: 970 1349 315 367 385 17 1452 86
Cuts at: 6913 6925 7271 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1349 970 385 367 346 315
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4162 4162
Size: 7276
BtgI C'CryG_G
Cuts at: 513 1065 2004 3866 513
Size: 552 939 1862 3923
Fragments arranged by size:
3923 1862 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6537
Fragments arranged by size:
6537 683 56
BtsI GCAGTG_nn'
Cuts at: 4792 5586 5606 4792
Size: 794 20 6462
Fragments arranged by size:
6462 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7241
DraI TTT'AAA
Cuts at: 2086 2256 2565 2985 3588 4320 4580 2086
Size: 170 309 420 603 732 260 4782
Fragments arranged by size:
4782 732 603 420 309 260 170
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6283 809
Size: 507 4967 1802
Fragments arranged by size:
4967 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2406 3460
Size: 54 1425 50 275 18 484 92 1054
Cuts at: 3460 4407 4723 5612 7241 8
Size: 947 316 889 1629 43
Fragments arranged by size:
1629 1425 1054 947 889 484 316 275
92 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4723 1537
Size: 275 2911 4090
Fragments arranged by size:
4090 2911 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2815 5206 1353
Size: 1462 2391 3423
Fragments arranged by size:
3423 2391 1462
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5863 6237 6383 387
Size: 1064 138 4274 374 146 1280
Fragments arranged by size:
4274 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4357 4357
Size: 7276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3092 3393 3525 4090 4443 5339 5936
Size: 393 1460 301 132 565 353 896 597
Cuts at: 5936 6723 6991 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1460 896 787 597 565 393 353
301 268 132
EcoO109I rG'GnC_Cy
Cuts at: 4327 4327
Size: 7276
EcoRI G'AATT_C
Cuts at: 2337 2773 4462 4585 4963 2337
Size: 436 1689 123 378 4650
Fragments arranged by size:
4650 1689 436 378 123
EcoRV GAT'ATC
Cuts at: 4344 4344
Size: 7276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 7276
FspI TGC'GCA
Cuts at: 5782 5782
Size: 7276
HaeII r_GCGC'y
Cuts at: 1678 2297 6423 1678
Size: 619 4126 2531
Fragments arranged by size:
4126 2531 619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3332 3364 4142 4174 4204
Size: 32 135 32 1760 32 778 32 30
Cuts at: 4204 4236 5917 5949 5991 6023 1373
Size: 32 1681 32 42 32 2626
Fragments arranged by size:
2626 1760 1681 778 135 42 32 32
32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 2833 3450 4399 4620 4866
Size: 988 281 223 672 617 949 221 246
Cuts at: 4866 669
Size: 3079
Fragments arranged by size:
3079 988 949 672 617 281 246 223
221
HindIII A'AGCT_T
Cuts at: 748 2113 748
Size: 1365 5911
HpaI GTT'AAC
Cuts at: 2161 2833 4399 4866 2161
Size: 672 1566 467 4571
Fragments arranged by size:
4571 1566 672 467
KpnI G_GTAC'C
Cuts at: 4601 4601
Size: 7276
MfeI C'AATT_G
Cuts at: 2040 2286 4875 2040
Size: 246 2589 4441
Fragments arranged by size:
4441 2589 246
MluI A'CGCG_T
Cuts at: 7152 7152
Size: 7276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3787 6390 6574 1305
Size: 2482 2603 184 2007
Fragments arranged by size:
2603 2482 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2316 2408 3462 4409 10
Size: 54 1425 343 484 92 1054 947 2877
Fragments arranged by size:
2877 1425 1054 947 484 343 92 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3331 3800 3806 3924 4371 4512 5234
Size: 648 2165 469 6 118 447 141 722
Cuts at: 5234 5593 5752 518
Size: 359 159 2042
Fragments arranged by size:
2165 2042 722 648 469 447 359 159
141 118 6
MspA1I CmG'CkG
Cuts at: 1606 2584 4692 5369 6517 6762 1606
Size: 978 2108 677 1148 245 2120
Fragments arranged by size:
2120 2108 1148 978 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3866 513
Size: 552 939 1862 3923
Fragments arranged by size:
3923 1862 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7276
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7276
NotI GC'GGCC_GC
Cuts at: 4723 4723
Size: 7276
NsiI A_TGCA'T
Cuts at: 3557 3557
Size: 7276
NspI r_CATG'y
Cuts at: 1132 1229 2743 4241 4634 6179 1132
Size: 97 1514 1498 393 1545 2229
Fragments arranged by size:
2229 1545 1514 1498 393 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7276
PciI A'CATG_T
Cuts at: 6175 6175
Size: 7276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7276
PmeI GTTT'AAAC
Cuts at: 4580 4580
Size: 7276
PmlI CAC'GTG
Cuts at: 2390 2390
Size: 7276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5345 5377 6858 6890 5345
Size: 32 1481 32 5731
Fragments arranged by size:
5731 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4327 4327
Size: 7276
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7276
PsiI TTA'TAA
Cuts at: 4846 4846
Size: 7276
PstI C_TGCA'G
Cuts at: 830 4628 830
Size: 3798 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7276
PvuII CAG'CTG
Cuts at: 2584 2584
Size: 7276
SacI G_AGCT'C
Cuts at: 4359 4359
Size: 7276
SalI G'TCGA_C
Cuts at: 1936 4618 1936
Size: 2682 4594
SanDI GG'GwC_CC
Cuts at: 4327 4327
Size: 7276
SbfI CC_TGCA'GG
Cuts at: 4628 4628
Size: 7276
ScaI AGT'ACT
Cuts at: 5524 7236 5524
Size: 1712 5564
SfcI C'TryA_G
Cuts at: 826 1045 2139 2511 4510 4624 5759 6440 6631
Size: 219 1094 372 1999 114 1135 681 191
Cuts at: 6631 826
Size: 1471
Fragments arranged by size:
1999 1471 1135 1094 681 372 219 191
114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7276
SmaI CCC'GGG
Cuts at: 1872 4603 1872
Size: 2731 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5380 6281 6543 820
Size: 197 926 3437 901 262 1553
Fragments arranged by size:
3437 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5067 493
Size: 4574 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7276
SphI G_CATG'C
Cuts at: 1132 4634 1132
Size: 3502 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5200 5
Size: 47 3771 1377 2081
Fragments arranged by size:
3771 2081 1377 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7276
StyI C'CwwG_G
Cuts at: 513 1065 2004 3378 3866 4410 4715 513
Size: 552 939 1374 488 544 305 3074
Fragments arranged by size:
3074 1374 939 552 544 488 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5303 5320 5473 5658 6103 325
Size: 1255 3723 17 153 185 445 1498
Fragments arranged by size:
3723 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3792 4176 5522
Size: 274 80 33 51 1443 1815 384 1346
Cuts at: 5522 7234 96
Size: 1712 138
Fragments arranged by size:
1815 1712 1443 1346 384 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7236 7268 7236
Size: 32 7244
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7276
XbaI T'CTAG_A
Cuts at: 2909 4612 2909
Size: 1703 5573
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7276
XmaI C'CCGG_G
Cuts at: 1870 4601 1870
Size: 2731 4545
XmnI GAAnn'nnTTC
Cuts at: 2295 2962 3577 5405 2295
Size: 667 615 1828 4166
Fragments arranged by size:
4166 1828 667 615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5084 276
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI