Restriction map for
pFN21AE2295
(Circular) (Six-base) MAPSORT of: pFN21AE2295.seq Check: 6016 from: 1 to: 11113
pFN21AE2295 11113 bp
With 182 enzymes: SgfI *
November 8, 2010 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6275 7957 8050 6275
Size: 1682 93 9338
Fragments arranged by size:
9338 1682 93
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8923 278
Size: 53 83 186 936 7387 2468
Fragments arranged by size:
7387 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 4549 8456 1937
Size: 1026 1586 3907 4594
Fragments arranged by size:
4594 3907 1586 1026
Acc65I G'GTAC_C
Cuts at: 8434 8434
Size: 11113
AclI AA'CG_TT
Cuts at: 1563 4467 9240 9613 1563
Size: 2904 4773 373 3063
Fragments arranged by size:
4773 3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2712 4242 7455 7554 7610 7917 8313 9176
Size: 1080 1530 3213 99 56 307 396 863
Cuts at: 9176 10560 10828 1632
Size: 1384 268 1917
Fragments arranged by size:
3213 1917 1530 1384 1080 863 396 307
268 99 56
AflII C'TTAA_G
Cuts at: 820 1017 2843 820
Size: 197 1826 9090
Fragments arranged by size:
9090 1826 197
AflIII A'CryG_T
Cuts at: 1203 4288 5330 6689 7238 8383 10012 10989 1203
Size: 3085 1042 1359 549 1145 1629 977 1327
Fragments arranged by size:
3085 1629 1359 1327 1145 1042 977 549
AgeI A'CCGG_T
Cuts at: 6419 9944 6419
Size: 3525 7588
AhdI GACnn_n'nnGTC
Cuts at: 3386 7942 9842 3386
Size: 4556 1900 4657
Fragments arranged by size:
4657 4556 1900
AleI CACnn'nnGTG
Cuts at: 1166 3683 3686 7790 1166
Size: 2517 3 4104 4489
Fragments arranged by size:
4489 4104 2517 3
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2273 2305 4231 4263 4605 4637 2273
Size: 32 1926 32 342 32 8749
Fragments arranged by size:
8749 1926 342 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4202 5158 6004 7688 8856 10428 4202
Size: 956 846 1684 1168 1572 4887
Fragments arranged by size:
4887 1684 1572 1168 956 846
ApaI G_GGCC'C
Cuts at: 8397 8397
Size: 11113
ApaLI G'TGCA_C
Cuts at: 6174 9170 10326 6174
Size: 2996 1156 6961
Fragments arranged by size:
6961 2996 1156
ApoI r'AATT_y
Cuts at: 1493 2251 2987 3460 4331 4346 4379 4723 5005
Size: 758 736 473 871 15 33 344 282
Cuts at: 5005 5035 5585 5593 5640 6155 6184 8313 8422
Size: 30 550 8 47 515 29 2129 109
Cuts at: 8422 8648 8800 10909 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2129 2109 1697 871 758 736 550 515
473 344 282 226 152 109 47 33
30 29 15 8
AseI AT'TA_AT
Cuts at: 160 1032 2159 7770 9667 160
Size: 872 1127 5611 1897 1606
Fragments arranged by size:
5611 1897 1606 1127 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11113
AvaI C'yCGr_G
Cuts at: 1870 1943 6939 7071 8438 1870
Size: 73 4996 132 1367 4545
Fragments arranged by size:
4996 4545 1367 132 73
AvrII C'CTAG_G
Cuts at: 3097 3097
Size: 11113
BamHI G'GATC_C
Cuts at: 2866 8443 8810 2866
Size: 5577 367 5169
Fragments arranged by size:
5577 5169 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6642 7216 7673 8434 9889
Size: 325 217 627 4855 574 457 761 1455
Cuts at: 9889 618
Size: 1842
Fragments arranged by size:
4855 1842 1455 761 627 574 457 325
217
BanII G_rGCy'C
Cuts at: 1391 3360 3422 3641 4421 6615 7764 8397 8432
Size: 1969 62 219 780 2194 1149 633 35
Cuts at: 8432 1391
Size: 4072
Fragments arranged by size:
4072 2194 1969 1149 780 633 219 62
35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3289 3809 4107 7568 7669 928
Size: 971 1390 520 298 3461 101 4372
Fragments arranged by size:
4372 3461 1390 971 520 298 101
BbvCI CC'TCA_GC
Cuts at: 7953 7953
Size: 11113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3041 3075 9304 9338 1903
Size: 34 1104 34 6229 34 3678
Fragments arranged by size:
6229 3678 1104 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9005 10215 9005
Size: 1210 9903
BclI T'GATC_A
Cuts at: 1548 4162 4444 4564 1548
Size: 2614 282 120 8097
Fragments arranged by size:
8097 2614 282 120
BfrBI ATG'CAT
Cuts at: 5995 5995
Size: 11113
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4688 9724 136
Size: 107 122 71 631 605 3016 5036 1525
Fragments arranged by size:
5036 3016 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3184 3855 11108 3184
Size: 671 7253 3189
Fragments arranged by size:
7253 3189 671
BlpI GC'TnA_GC
Cuts at: 8530 8530
Size: 11113
Bme1580I G_kGCm'C
Cuts at: 1790 5369 6178 6645 8074 8397 9174 10330 1790
Size: 3579 809 467 1429 323 777 1156 2573
Fragments arranged by size:
3579 2573 1429 1156 809 777 467 323
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11113
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3146 3596 7517
Size: 416 53 83 399 24 1729 450 3921
Cuts at: 7517 8844 9802 442
Size: 1327 958 1753
Fragments arranged by size:
3921 1753 1729 1327 958 450 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4744 7180 4744
Size: 2436 8677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2256 2887 3456 3935 6882 7524 9773 1239
Size: 1017 631 569 479 2947 642 2249 2579
Fragments arranged by size:
2947 2579 2249 1017 642 631 569 479
Bpu10I CC'TnA_GC
Cuts at: 2736 3354 3421 7953 2736
Size: 618 67 4532 5896
Fragments arranged by size:
5896 4532 618 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2775 4436 5055 7089 7836 9238 10103 10401 2775
Size: 1661 619 2034 747 1402 865 298 3487
Fragments arranged by size:
3487 2034 1661 1402 865 747 619 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3170 7415 9776 882
Size: 617 1671 4245 2361 2219
Fragments arranged by size:
4245 2361 2219 1671 617
BsaAI yAC'GTr
Cuts at: 493 1204 4168 8904 493
Size: 711 2964 4736 2702
Fragments arranged by size:
4736 2964 2702 711
BsaBI GATnn'nnATC
Cuts at: 2609 5109 11107 2609
Size: 2500 5998 2615
Fragments arranged by size:
5998 2615 2500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8920 9302 9937 275
Size: 53 83 186 936 7387 382 635 1451
Fragments arranged by size:
7387 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6043 6419 9546 9944 10218 10365 10776 6043
Size: 376 3127 398 274 147 411 6380
Fragments arranged by size:
6380 3127 411 398 376 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 4231 4261 6356
Size: 30 165 30 1482 30 1316 30 2095
Cuts at: 6356 6386 7567 7597 7817 7847 1178
Size: 30 1181 30 220 30 4444
Fragments arranged by size:
4444 2095 1482 1316 1181 220 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3423 3709 3893 5902 7506 7719 7839 7970
Size: 2237 286 184 2009 1604 213 120 131
Cuts at: 7970 1186
Size: 4329
Fragments arranged by size:
4329 2237 2009 1604 286 213 184 131
120
BseYI C'CCAG_C
Cuts at: 2801 5904 10316 10812 2801
Size: 3103 4412 496 3102
Fragments arranged by size:
4412 3103 3102 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 8069 1163
Size: 573 6333 4207
Fragments arranged by size:
6333 4207 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6598 8563 9324 10352 1540
Size: 275 186 4597 1965 761 1028 2301
Fragments arranged by size:
4597 2301 1965 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2755 3641 4421 6178 6558 6615 7012 8001 8109
Size: 886 780 1757 380 57 397 989 108
Cuts at: 8109 9174 9259 10330 2755
Size: 1065 85 1071 3538
Fragments arranged by size:
3538 1757 1071 1065 989 886 780 397
380 108 85 57
BsmI GAATG_Cn'
Cuts at: 2805 3824 5831 5881 7891 8624 8717 2805
Size: 1019 2007 50 2010 733 93 5201
Fragments arranged by size:
5201 2010 2007 1019 733 93 50
BsmBI CGTCTCn'nnnn_
Cuts at: 2973 2973
Size: 11113
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2755 3360 3422 3641 4421 5369 6178
Size: 399 965 605 62 219 780 948 809
Cuts at: 6178 6558 6615 6645 7012 7764 8001 8074 8109
Size: 380 57 30 367 752 237 73 35
Cuts at: 8109 8397 8432 9174 9259 10330 1391
Size: 288 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 965 948 809 780 752 742
605 399 380 367 288 237 219 85
73 62 57 35 35 30
BspEI T'CCGG_A
Cuts at: 10776 10776
Size: 11113
BspHI T'CATG_A
Cuts at: 6028 9002 10732 6028
Size: 2974 1730 6409
Fragments arranged by size:
6409 2974 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 3499 5962 6275 7957 8050 8468 844
Size: 1462 1193 2463 313 1682 93 418 3489
Fragments arranged by size:
3489 2463 1682 1462 1193 418 313 93
BsrBI CCG'CTC
Cuts at: 1394 1823 7086 7514 9000 1394
Size: 429 5263 428 1486 3507
Fragments arranged by size:
5263 3507 1486 429 428
BsrDI GCAATG_nn'
Cuts at: 65 1248 2308 3496 6011 9608 9782 65
Size: 1183 1060 1188 2515 3597 174 1396
Fragments arranged by size:
3597 2515 1396 1188 1183 1060 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6419 6597 9757 9944 1700
Size: 110 4609 178 3160 187 2869
Fragments arranged by size:
4609 3160 2869 187 178 110
BsrGI T'GTAC_A
Cuts at: 96 2527 4261 4291 5338 5395 6414 6686 96
Size: 2431 1734 30 1047 57 1019 272 4523
Fragments arranged by size:
4523 2431 1734 1047 1019 272 57 30
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11113
BssSI C'ACGA_G
Cuts at: 2829 9173 10185 2829
Size: 6344 1012 3757
Fragments arranged by size:
6344 3757 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3828 5116 5562 5992 7688 794
Size: 3034 1288 446 430 1696 4219
Fragments arranged by size:
4219 3034 1696 1288 446 430
BstBI TT'CG_AA
Cuts at: 3051 9917 3051
Size: 6866 4247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11113
BstXI CCAn_nnnn'nTGG
Cuts at: 3129 4487 3129
Size: 1358 9755
BstYI r'GATC_y
Cuts at: 1972 2640 2866 3184 3855 4613 8443 8810 9195
Size: 668 226 318 671 758 3830 367 385
Cuts at: 9195 9212 10664 10750 10762 11108 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3830 1977 1452 758 671 668 385 367
346 318 226 86 17 12
BstZ17I GTA'TAC
Cuts at: 4550 4550
Size: 11113
Bsu36I CC'TnA_GG
Cuts at: 7405 7405
Size: 11113
BtgI C'CryG_G
Cuts at: 513 1065 4017 513
Size: 552 2952 7609
Fragments arranged by size:
7609 2952 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3787 5964 7298 8020 487
Size: 683 56 2561 2177 1334 722 3580
Fragments arranged by size:
3580 2561 2177 1334 722 683 56
BtsI GCAGTG_nn'
Cuts at: 4392 6487 6987 7326 7394 8629 9423 9443 4392
Size: 2095 500 339 68 1235 794 20 6062
Fragments arranged by size:
6062 2095 1235 794 500 339 68 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8083 8118 529
Size: 35 7519 35 3524
Fragments arranged by size:
7519 3524 35 35
DraI TTT'AAA
Cuts at: 3969 4327 8417 3969
Size: 358 4090 6665
Fragments arranged by size:
6665 4090 358
DraIII CAC_nnn'GTG
Cuts at: 2672 2748 7525 2672
Size: 76 4777 6260
Fragments arranged by size:
6260 4777 76
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2495 3293 8354 10120 809
Size: 507 1179 798 5061 1766 1802
Fragments arranged by size:
5061 1802 1766 1179 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6591 8031 8560
Size: 54 1425 50 275 18 4761 1440 529
Cuts at: 8560 9449 11078 8
Size: 889 1629 43
Fragments arranged by size:
4761 1629 1440 1425 889 529 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8560 1537
Size: 275 6748 4090
Fragments arranged by size:
6748 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2688 2811 3578 3727 6114 6588 7268 7870
Size: 1335 123 767 149 2387 474 680 602
Cuts at: 7870 9043 1353
Size: 1173 3423
Fragments arranged by size:
3423 2387 1335 1173 767 680 602 474
149 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5494 9700 10074 10220 387
Size: 1064 138 3905 4206 374 146 1280
Fragments arranged by size:
4206 3905 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3639 4419 6613 3639
Size: 780 2194 8139
Fragments arranged by size:
8139 2194 780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2712 2887 3456 3935 4242 6882
Size: 393 624 456 175 569 479 307 2640
Cuts at: 6882 7455 7524 7554 7610 7917 8313 9176 9773
Size: 573 69 30 56 307 396 863 597
Cuts at: 9773 10560 10828 1239
Size: 787 268 1524
Fragments arranged by size:
2640 1524 863 787 624 597 573 569
479 456 396 393 307 307 268 175
69 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3101 3564 3101
Size: 463 10650
EcoO109I rG'GnC_Cy
Cuts at: 3166 3939 4690 7210 8393 8394 3166
Size: 773 751 2520 1183 1 5885
Fragments arranged by size:
5885 2520 1183 773 751 1
EcoRI G'AATT_C
Cuts at: 4346 4723 8422 8800 4346
Size: 377 3699 378 6659
Fragments arranged by size:
6659 3699 378 377
EcoRV GAT'ATC
Cuts at: 5744 5744
Size: 11113
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3035 3275 3035
Size: 240 10873
FspI TGC'GCA
Cuts at: 9619 9619
Size: 11113
HaeII r_GCGC'y
Cuts at: 1678 2321 10260 1678
Size: 643 7939 2531
Fragments arranged by size:
7939 2531 643
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3074 3106 3169 3201 3372
Size: 32 135 32 1502 32 63 32 171
Cuts at: 3372 3404 3765 3797 4429 4461 4742 4774 5068
Size: 32 361 32 632 32 281 32 294
Cuts at: 5068 5100 5177 5209 6651 6683 6800 6832 7070
Size: 32 77 32 1442 32 117 32 238
Cuts at: 7070 7102 7847 7879 7898 7928 7930 7960 7970
Size: 32 745 32 19 30 2 30 10
Cuts at: 7970 8002 9754 9786 9828 9860 1373
Size: 32 1752 32 42 32 2626
Fragments arranged by size:
2626 1752 1502 1442 745 632 361 294
281 238 171 135 117 77 63 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 19 10 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3676 4158 4228 5247 5868 8457
Size: 988 281 1738 482 70 1019 621 2589
Cuts at: 8457 8703 669
Size: 246 3079
Fragments arranged by size:
3079 2589 1738 1019 988 621 482 281
246 70
HindIII A'AGCT_T
Cuts at: 748 3520 5844 7139 748
Size: 2772 2324 1295 4722
Fragments arranged by size:
4722 2772 2324 1295
HpaI GTT'AAC
Cuts at: 4158 5868 8703 4158
Size: 1710 2835 6568
Fragments arranged by size:
6568 2835 1710
KpnI G_GTAC'C
Cuts at: 8438 8438
Size: 11113
MfeI C'AATT_G
Cuts at: 3321 5092 6453 8712 3321
Size: 1771 1361 2259 5722
Fragments arranged by size:
5722 2259 1771 1361
MluI A'CGCG_T
Cuts at: 10989 10989
Size: 11113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5844 6560 8150 10227 10411 1305
Size: 3657 882 716 1590 2077 184 2007
Fragments arranged by size:
3657 2077 2007 1590 882 716 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 8033 10
Size: 54 1425 343 6201 3090
Fragments arranged by size:
6201 3090 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2421 3127 3683 3686 4121 6563 7790
Size: 648 1255 706 556 3 435 2442 1227
Cuts at: 7790 7974 8349 9071 9430 9589 518
Size: 184 375 722 359 159 2042
Fragments arranged by size:
2442 2042 1255 1227 722 706 648 556
435 375 359 184 159 3
MspA1I CmG'CkG
Cuts at: 1606 2636 4307 6273 7685 8529 9206 10354 10599
Size: 1030 1671 1966 1412 844 677 1148 245
Cuts at: 10599 1606
Size: 2120
Fragments arranged by size:
2120 1966 1671 1412 1148 1030 844 677
245
NcoI C'CATG_G
Cuts at: 513 1065 4017 513
Size: 552 2952 7609
Fragments arranged by size:
7609 2952 552
NdeI CA'TA_TG
Cuts at: 387 3376 387
Size: 2989 8124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11113
NotI GC'GGCC_GC
Cuts at: 8560 8560
Size: 11113
NsiI A_TGCA'T
Cuts at: 5997 5997
Size: 11113
NspI r_CATG'y
Cuts at: 1132 1229 4292 5334 6693 8471 10016 1132
Size: 97 3063 1042 1359 1778 1545 2229
Fragments arranged by size:
3063 2229 1778 1545 1359 1042 97
PasI CC'CwG_GG
Cuts at: 1348 3169 1348
Size: 1821 9292
PciI A'CATG_T
Cuts at: 4288 5330 6689 10012 4288
Size: 1042 1359 3323 5389
Fragments arranged by size:
5389 3323 1359 1042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3158 3998 4610 5158 5661 1108
Size: 2050 840 612 548 503 6560
Fragments arranged by size:
6560 2050 840 612 548 503
PmeI GTTT'AAAC
Cuts at: 8417 8417
Size: 11113
PmlI CAC'GTG
Cuts at: 4168 4168
Size: 11113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4231 4263 9182 9214 10695 10727 4231
Size: 32 4919 32 1481 32 4617
Fragments arranged by size:
4919 4617 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 7210 7210
Size: 11113
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11113
PsiI TTA'TAA
Cuts at: 2430 3923 4377 5850 6885 7137 8683 2430
Size: 1493 454 1473 1035 252 1546 4860
Fragments arranged by size:
4860 1546 1493 1473 1035 454 252
PspOMI G'GGCC_C
Cuts at: 8393 8393
Size: 11113
PstI C_TGCA'G
Cuts at: 830 2635 4502 6272 8465 830
Size: 1805 1867 1770 2193 3478
Fragments arranged by size:
3478 2193 1867 1805 1770
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11113
PvuII CAG'CTG
Cuts at: 2636 4307 6273 7685 2636
Size: 1671 1966 1412 6064
Fragments arranged by size:
6064 1966 1671 1412
SacI G_AGCT'C
Cuts at: 3641 4421 6615 3641
Size: 780 2194 8139
Fragments arranged by size:
8139 2194 780
SalI G'TCGA_C
Cuts at: 1936 8455 1936
Size: 6519 4594
SbfI CC_TGCA'GG
Cuts at: 8465 8465
Size: 11113
ScaI AGT'ACT
Cuts at: 9361 11073 9361
Size: 1712 9401
SfcI C'TryA_G
Cuts at: 826 1045 2631 4024 4498 6268 8461 9596 10277
Size: 219 1586 1393 474 1770 2193 1135 681
Cuts at: 10277 10468 826
Size: 191 1471
Fragments arranged by size:
2193 1770 1586 1471 1393 1135 681 474
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11113
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11113
SmaI CCC'GGG
Cuts at: 1872 8440 1872
Size: 6568 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2790 2843 4451 5070 7071 7104
Size: 197 926 847 53 1608 619 2001 33
Cuts at: 7104 7851 9217 10118 10380 820
Size: 747 1366 901 262 1553
Fragments arranged by size:
2001 1608 1553 1366 926 901 847 747
619 262 197 53 33
SnaBI TAC'GTA
Cuts at: 493 8904 493
Size: 8411 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11113
SphI G_CATG'C
Cuts at: 1132 8471 1132
Size: 7339 3774
SspI AAT'ATT
Cuts at: 5 52 4339 4991 8020 9037 5
Size: 47 4287 652 3029 1017 2081
Fragments arranged by size:
4287 3029 2081 1017 652 47
StuI AGG'CCT
Cuts at: 1517 2029 3047 3107 8243 1517
Size: 512 1018 60 5136 4387
Fragments arranged by size:
5136 4387 1018 512 60
StyI C'CwwG_G
Cuts at: 513 1065 2097 3019 3097 3217 4017 6322 7167
Size: 552 1032 922 78 120 800 2305 845
Cuts at: 7167 8552 513
Size: 1385 3074
Fragments arranged by size:
3074 2305 1385 1032 922 845 800 552
120 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2507 3161 6435 7082 7536 9140 9157
Size: 1255 927 654 3274 647 454 1604 17
Cuts at: 9157 9310 9495 9940 325
Size: 153 185 445 1498
Fragments arranged by size:
3274 1604 1498 1255 927 654 647 454
445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2527 4261 4291
Size: 274 80 33 51 1443 550 1734 30
Cuts at: 4291 5309 5338 5395 6414 6686 6753 9359 11071
Size: 1018 29 57 1019 272 67 2606 1712
Cuts at: 11071 96
Size: 138
Fragments arranged by size:
2606 1734 1712 1443 1019 1018 550 274
272 138 80 67 57 51 33 30
29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2656 2688 2732 2764 6084 6116 7038 7070 7567
Size: 32 44 32 3320 32 922 32 497
Cuts at: 7567 7599 7817 7849 11073 11105 2656
Size: 32 218 32 3224 32 2664
Fragments arranged by size:
3320 3224 2664 922 497 218 44 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11113
XbaI T'CTAG_A
Cuts at: 8449 8449
Size: 11113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 6459 7174 7210 7548 2113
Size: 4346 715 36 338 5678
Fragments arranged by size:
5678 4346 715 338 36
XhoI C'TCGA_G
Cuts at: 1943 7071 1943
Size: 5128 5985
XmaI C'CCGG_G
Cuts at: 1870 8438 1870
Size: 6568 4545
XmnI GAAnn'nnTTC
Cuts at: 5831 6495 9242 5831
Size: 664 2747 7702
Fragments arranged by size:
7702 2747 664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8921 276
Size: 53 83 186 936 7387 2468
Fragments arranged by size:
7387 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI