Restriction map for
pFN21AE2288
(Circular) (Six-base) MAPSORT of: pFN21AE2288.seq Check: 1523 from: 1 to: 5566
pFN21AE2288 5566 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2909 1937
Size: 972 4594
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 3629 5013 5281 1632
Size: 617 1380 1384 268 1917
Fragments arranged by size:
1917 1384 1380 617 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 2514 1166
Size: 1348 4218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5566
AlwNI CAG_nnn'CTG
Cuts at: 3309 4881 3309
Size: 1572 3994
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 1870
Size: 73 948 4545
Fragments arranged by size:
4545 948 73
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2887 4342 618
Size: 325 217 627 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2606 2885 1391
Size: 1215 279 4072
Fragments arranged by size:
4072 1215 279
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 928
Size: 971 331 4264
Fragments arranged by size:
4264 971 331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 2187 1548
Size: 639 4927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4177 136
Size: 107 122 71 631 605 2505 1525
Fragments arranged by size:
2505 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2446 2983 2446
Size: 537 5029
Bme1580I G_kGCm'C
Cuts at: 1790 2322 3627 4783 1790
Size: 532 1305 1156 2573
Fragments arranged by size:
2573 1305 1156 532
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2619 3297 4255
Size: 416 53 83 399 24 1202 678 958
Cuts at: 4255 442
Size: 1753
Fragments arranged by size:
1753 1202 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2827 4226 1239
Size: 1130 458 1399 2579
Fragments arranged by size:
2579 1399 1130 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2205 2439 3357 493
Size: 711 1001 234 918 2702
Fragments arranged by size:
2702 1001 918 711 234
BsaBI GATnn'nnATC
Cuts at: 5560 5560
Size: 5566
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3755 4390 275
Size: 53 83 186 936 1840 382 635 1451
Fragments arranged by size:
1840 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2331 3999 4397 4671 4818 5229 2331
Size: 1668 398 274 147 411 2668
Fragments arranged by size:
2668 1668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5341
Fragments arranged by size:
5341 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5566
BseYI C'CCAG_C
Cuts at: 2178 4769 5265 2178
Size: 2591 496 2479
Fragments arranged by size:
2591 2479 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2461 1163
Size: 573 725 4268
Fragments arranged by size:
4268 725 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3016 3777 4805 1540
Size: 275 186 1015 761 1028 2301
Fragments arranged by size:
2301 1028 1015 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 3627 3712 4783 2043
Size: 1584 85 1071 2826
Fragments arranged by size:
2826 1584 1071 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2322 2606 2885 3627 3712 4783
Size: 399 253 279 284 279 742 85 1071
Cuts at: 4783 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 284 279 279 253
85
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2921 844
Size: 2077 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 1394
Size: 429 1630 3507
Fragments arranged by size:
3507 1630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4210 4397 1700
Size: 110 2400 187 2869
Fragments arranged by size:
2869 2400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5566
BssSI C'ACGA_G
Cuts at: 2717 3626 4638 2717
Size: 909 1012 3645
Fragments arranged by size:
3645 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2132 2571 2896 3263 3648 3665 5117 5203
Size: 160 439 325 367 385 17 1452 86
Cuts at: 5203 5215 5561 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 439 385 367 346 325 160
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2700 513
Size: 552 939 696 3379
Fragments arranged by size:
3379 939 696 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 3082 3876 3896 3082
Size: 794 20 4752
Fragments arranged by size:
4752 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DraIII CAC_nnn'GTG
Cuts at: 2855 2855
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 4573 809
Size: 507 1245 2012 1802
Fragments arranged by size:
2012 1802 1245 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 2529 2697
Size: 54 1425 50 275 18 648 51 168
Cuts at: 2697 2730 3013 3902 5531 8
Size: 33 283 889 1629 43
Fragments arranged by size:
1629 1425 889 648 283 275 168 54
51 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4153 4527 4673 387
Size: 1064 138 2564 374 146 1280
Fragments arranged by size:
2564 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2369 2827 3629 4226 5013 5281
Size: 393 617 120 458 802 597 787 268
Cuts at: 5281 1239
Size: 1524
Fragments arranged by size:
1524 802 787 617 597 458 393 268
120
EcoO109I rG'GnC_Cy
Cuts at: 2546 2546
Size: 5566
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2296 4713 1678
Size: 618 2417 2531
Fragments arranged by size:
2531 2417 618
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2700 2732 4207 4239 4281
Size: 32 135 32 1128 32 1475 32 42
Cuts at: 4281 4313 1373
Size: 32 2626
Fragments arranged by size:
2626 1475 1128 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2910 3156 669
Size: 988 281 972 246 3079
Fragments arranged by size:
3079 988 972 281 246
HindIII A'AGCT_T
Cuts at: 748 2166 748
Size: 1418 4148
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4680 4864 1305
Size: 3375 184 2007
Fragments arranged by size:
3375 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 2732 10
Size: 54 1425 343 648 252 2844
Fragments arranged by size:
2844 1425 648 343 252 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2699 3524 3883 4042 518
Size: 648 1348 185 825 359 159 2042
Fragments arranged by size:
2042 1348 825 648 359 185 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2982 3659 4807 5052 1606
Size: 576 800 677 1148 245 2120
Fragments arranged by size:
2120 1148 800 677 576 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4075
Fragments arranged by size:
4075 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2924 4469 1132
Size: 97 1695 1545 2229
Fragments arranged by size:
2229 1695 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5566
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PmlI CAC'GTG
Cuts at: 2439 2439
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 5148 5180 3635
Size: 32 1481 32 4021
Fragments arranged by size:
4021 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 2546
Size: 5566
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2395 2918 830
Size: 1565 523 3478
Fragments arranged by size:
3478 1565 523
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5566
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2323 2391 2575 2914 4049 4730 4921
Size: 219 1278 68 184 339 1135 681 191
Cuts at: 4921 826
Size: 1471
Fragments arranged by size:
1471 1278 1135 681 339 219 191 184
68
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2893 1872
Size: 1021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 2428 3490 5
Size: 47 2376 1062 2081
Fragments arranged by size:
2376 2081 1062 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2481 2634 3005 513
Size: 552 939 120 357 153 371 3074
Fragments arranged by size:
3074 939 552 371 357 153 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3812 5524 96
Size: 274 80 33 51 1443 1835 1712 138
Fragments arranged by size:
1835 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2632 2539
Size: 93 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2891 1870
Size: 1021 4545
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI