Restriction map for
pFN21AE2276
(Circular) (Six-base) MAPSORT of: pFN21AE2276.seq Check: 2397 from: 1 to: 6397
pFN21AE2276 6397 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4207 278
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3740 1937
Size: 1803 4594
Acc65I G'GTAC_C
Cuts at: 3069 3718 3069
Size: 649 5748
AclI AA'CG_TT
Cuts at: 1563 4524 4897 1563
Size: 2961 373 3063
Fragments arranged by size:
3063 2961 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 2834 3359 4460 5844 6112 1632
Size: 454 748 525 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1101 748 525 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6200
AflIII A'CryG_T
Cuts at: 1203 5296 6273 1203
Size: 4093 977 1327
Fragments arranged by size:
4093 1327 977
AgeI A'CCGG_T
Cuts at: 5228 5228
Size: 6397
AhdI GACnn_n'nnGTC
Cuts at: 5126 5126
Size: 6397
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3162 1184
Size: 1978 4419
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 6365
AlwNI CAG_nnn'CTG
Cuts at: 2632 2737 3175 3325 3355 4140 5712 2632
Size: 105 438 150 30 785 1572 3317
Fragments arranged by size:
3317 1572 785 438 150 105 30
ApaI G_GGCC'C
Cuts at: 2378 2378
Size: 6397
ApaLI G'TGCA_C
Cuts at: 4454 5610 4454
Size: 1156 5241
ApoI r'AATT_y
Cuts at: 1493 2801 3706 3932 4084 6193 1493
Size: 1308 905 226 152 2109 1697
Fragments arranged by size:
2109 1697 1308 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4951 160
Size: 872 3919 1606
Fragments arranged by size:
3919 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
AvaI C'yCGr_G
Cuts at: 1870 1943 3722 1870
Size: 73 1779 4545
Fragments arranged by size:
4545 1779 73
BamHI G'GATC_C
Cuts at: 3727 4094 3727
Size: 367 6030
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2288 2609 3069 3500 3718
Size: 325 217 627 501 321 460 431 218
Cuts at: 3718 5173 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 501 460 431 325 321
218 217
BanII G_rGCy'C
Cuts at: 1391 2204 2378 3716 1391
Size: 813 174 1338 4072
Fragments arranged by size:
4072 1338 813 174
BbeI G_GCGC'C
Cuts at: 2292 2292
Size: 6397
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5426
BbvCI CC'TCA_GC
Cuts at: 3009 3054 3620 3009
Size: 45 566 5786
Fragments arranged by size:
5786 566 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4588 4622 1903
Size: 34 2651 34 3678
Fragments arranged by size:
3678 2651 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4289 5499 4289
Size: 1210 5187
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2097 2148 2199
Size: 107 122 71 631 605 425 51 51
Cuts at: 2199 5008 136
Size: 2809 1525
Fragments arranged by size:
2809 1525 631 605 425 122 107 71
51 51
BglII A'GATC_T
Cuts at: 3179 6392 3179
Size: 3213 3184
BlpI GC'TnA_GC
Cuts at: 3240 3814 3240
Size: 574 5823
Bme1580I G_kGCm'C
Cuts at: 1790 2378 2396 2612 4458 5614 1790
Size: 588 18 216 1846 1156 2573
Fragments arranged by size:
2573 1846 1156 588 216 18
BmgBI CAC'GTC
Cuts at: 1318 3196 1318
Size: 1878 4519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3618 4128 5086
Size: 416 53 83 399 24 2201 510 958
Cuts at: 5086 442
Size: 1753
Fragments arranged by size:
2201 1753 958 510 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2421 2654 3314 3431 3469 3658 5057
Size: 871 311 233 660 117 38 189 1399
Cuts at: 5057 1239
Size: 2579
Fragments arranged by size:
2579 1399 871 660 311 233 189 117
38
Bpu10I CC'TnA_GC
Cuts at: 3009 3054 3523 3620 3009
Size: 45 469 97 5786
Fragments arranged by size:
5786 469 97 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2735 3248 4522 5387 5685 2735
Size: 513 1274 865 298 3447
Fragments arranged by size:
3447 1274 865 513 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5060 882
Size: 617 3561 2219
Fragments arranged by size:
3561 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4188 493
Size: 711 2984 2702
Fragments arranged by size:
2984 2702 711
BsaBI GATnn'nnATC
Cuts at: 6391 6391
Size: 6397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2289 4204 4586 5221
Size: 53 83 186 936 756 1915 382 635
Cuts at: 5221 275
Size: 1451
Fragments arranged by size:
1915 1451 936 756 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4830 5228 5502 5649 6060 4830
Size: 398 274 147 411 5167
Fragments arranged by size:
5167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2627 2657 1178
Size: 30 165 30 1224 30 4918
Fragments arranged by size:
4918 1224 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2504 3044 3058 3101 3434 3658 3661
Size: 989 329 540 14 43 333 224 3
Cuts at: 3661 1186
Size: 3922
Fragments arranged by size:
3922 989 540 333 329 224 43 14
3
BseYI C'CCAG_C
Cuts at: 5600 6096 5600
Size: 496 5901
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3445 3491 1163
Size: 573 1709 46 4069
Fragments arranged by size:
4069 1709 573 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3847 4608 5636 1540
Size: 275 186 1846 761 1028 2301
Fragments arranged by size:
2301 1846 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4458 4543 5614 4458
Size: 85 1071 5241
Fragments arranged by size:
5241 1071 85
BsmI GAATG_Cn'
Cuts at: 3908 4001 3908
Size: 93 6304
BsmBI CGTCTCn'nnnn_
Cuts at: 3487 3487
Size: 6397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2378 2396 2612 3716 4458 4543
Size: 399 414 174 18 216 1104 742 85
Cuts at: 4543 5614 1391
Size: 1071 2174
Fragments arranged by size:
2174 1104 1071 742 414 399 216 174
85 18
BspEI T'CCGG_A
Cuts at: 6060 6060
Size: 6397
BspHI T'CATG_A
Cuts at: 4286 6016 4286
Size: 1730 4667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 3132 3752 844
Size: 1616 672 620 3489
Fragments arranged by size:
3489 1616 672 620
BsrBI CCG'CTC
Cuts at: 1394 1823 2094 2124 4284 1394
Size: 429 271 30 2160 3507
Fragments arranged by size:
3507 2160 429 271 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 3228 4892 5066 65
Size: 1183 1980 1664 174 1396
Fragments arranged by size:
1980 1664 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 2351 5041 5228 1700
Size: 110 511 30 2690 187 2869
Fragments arranged by size:
2869 2690 511 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6397
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6397
BssSI C'ACGA_G
Cuts at: 4457 5469 4457
Size: 1012 5385
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6397
BstBI TT'CG_AA
Cuts at: 5201 5201
Size: 6397
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6397
BstYI r'GATC_y
Cuts at: 1972 2211 3179 3727 4094 4479 4496 5948 6034
Size: 239 968 548 367 385 17 1452 86
Cuts at: 6034 6046 6392 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 968 548 385 367 346 239
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2666 2666
Size: 6397
BtgI C'CryG_G
Cuts at: 513 1065 2004 2955 2967 513
Size: 552 939 951 12 3943
Fragments arranged by size:
3943 951 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2989 487
Size: 683 56 1763 3895
Fragments arranged by size:
3895 1763 683 56
BtsI GCAGTG_nn'
Cuts at: 2140 3212 3523 3565 3913 4707 4727 2140
Size: 1072 311 42 348 794 20 3810
Fragments arranged by size:
3810 1072 794 348 311 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6362
DraI TTT'AAA
Cuts at: 3701 3701
Size: 6397
DraIII CAC_nnn'GTG
Cuts at: 2586 2586
Size: 6397
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5404 809
Size: 507 4088 1802
Fragments arranged by size:
4088 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3844 4733
Size: 54 1425 50 275 18 177 1837 889
Cuts at: 4733 6362 8
Size: 1629 43
Fragments arranged by size:
1837 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3844 1537
Size: 275 2032 4090
Fragments arranged by size:
4090 2032 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3677 4327 1353
Size: 2324 650 3423
Fragments arranged by size:
3423 2324 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4984 5358 5504 387
Size: 1064 138 3395 374 146 1280
Fragments arranged by size:
3395 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2110 2421 2654 2834 3314 3359
Size: 393 454 24 311 233 180 480 45
Cuts at: 3359 3431 3469 3658 4460 5057 5844 6112 1239
Size: 72 38 189 802 597 787 268 1524
Fragments arranged by size:
1524 802 787 597 480 454 393 311
268 233 189 180 72 45 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2741 3308 3676 2597
Size: 144 567 368 5318
Fragments arranged by size:
5318 567 368 144
EcoO109I rG'GnC_Cy
Cuts at: 2374 2375 2734 2746 3322 2374
Size: 1 359 12 576 5449
Fragments arranged by size:
5449 576 359 12 1
EcoRI G'AATT_C
Cuts at: 3706 4084 3706
Size: 378 6019
FspI TGC'GCA
Cuts at: 3128 4903 3128
Size: 1775 4622
HaeII r_GCGC'y
Cuts at: 1678 2292 5544 1678
Size: 614 3252 2531
Fragments arranged by size:
3252 2531 614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3047 3079 3171 3203 5038
Size: 32 135 32 1475 32 92 32 1835
Cuts at: 5038 5070 5112 5144 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1835 1475 135 92 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3457 3741 3987 669
Size: 988 281 487 1032 284 246 3079
Fragments arranged by size:
3079 1032 988 487 284 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6397
HpaI GTT'AAC
Cuts at: 3987 3987
Size: 6397
KasI G'GCGC_C
Cuts at: 2288 2288
Size: 6397
KpnI G_GTAC'C
Cuts at: 3073 3722 3073
Size: 649 5748
MfeI C'AATT_G
Cuts at: 3996 3996
Size: 6397
MluI A'CGCG_T
Cuts at: 6273 6273
Size: 6397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3427 3553 3610 5511 5695 1305
Size: 2122 126 57 1901 184 2007
Fragments arranged by size:
2122 2007 1901 184 126 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4398
Fragments arranged by size:
4398 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 2954 4355 4714 4873 518
Size: 648 948 840 1401 359 159 2042
Fragments arranged by size:
2042 1401 948 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2232 2476 2677 3507 3597 3813 4490 5638
Size: 626 244 201 830 90 216 677 1148
Cuts at: 5638 5883 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 830 677 626 245 244 216
201 90
NaeI GCC'GGC
Cuts at: 2323 2353 2323
Size: 30 6367
NarI GG'CG_CC
Cuts at: 2289 2289
Size: 6397
NcoI C'CATG_G
Cuts at: 513 1065 2004 2967 513
Size: 552 939 963 3943
Fragments arranged by size:
3943 963 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6397
NgoMIV G'CCGG_C
Cuts at: 2321 2351 2321
Size: 30 6367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6397
NotI GC'GGCC_GC
Cuts at: 3844 3844
Size: 6397
NspI r_CATG'y
Cuts at: 1132 1229 2157 2731 3755 5300 1132
Size: 97 928 574 1024 1545 2229
Fragments arranged by size:
2229 1545 1024 928 574 97
PasI CC'CwG_GG
Cuts at: 1348 2081 1348
Size: 733 5664
PciI A'CATG_T
Cuts at: 5296 5296
Size: 6397
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6397
PmeI GTTT'AAAC
Cuts at: 3701 3701
Size: 6397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 4466 4498 5979 6011 2627
Size: 32 1807 32 1481 32 3013
Fragments arranged by size:
3013 1807 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2734 2746 3322 2734
Size: 12 576 5809
Fragments arranged by size:
5809 576 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6397
PsiI TTA'TAA
Cuts at: 3967 3967
Size: 6397
PspOMI G'GGCC_C
Cuts at: 2374 2374
Size: 6397
PstI C_TGCA'G
Cuts at: 830 2601 3096 3309 3749 830
Size: 1771 495 213 440 3478
Fragments arranged by size:
3478 1771 495 440 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6397
PvuII CAG'CTG
Cuts at: 2232 2677 2232
Size: 445 5952
SalI G'TCGA_C
Cuts at: 1936 3739 1936
Size: 1803 4594
SapI GCTCTTCn'nnn_
Cuts at: 3677 3677
Size: 6397
SbfI CC_TGCA'GG
Cuts at: 3749 3749
Size: 6397
ScaI AGT'ACT
Cuts at: 4645 6357 4645
Size: 1712 4685
SexAI A'CCwGG_T
Cuts at: 2736 2736
Size: 6397
SfcI C'TryA_G
Cuts at: 826 1045 2597 3092 3218 3305 3465 3745 4880
Size: 219 1552 495 126 87 160 280 1135
Cuts at: 4880 5561 5752 826
Size: 681 191 1471
Fragments arranged by size:
1552 1471 1135 681 495 280 219 191
160 126 87
SfoI GGC'GCC
Cuts at: 2290 2290
Size: 6397
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6397
SmaI CCC'GGG
Cuts at: 1872 3724 1872
Size: 1852 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 3263 4501 5402 5664 820
Size: 197 926 807 513 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 807 513 262 197
SnaBI TAC'GTA
Cuts at: 493 4188 493
Size: 3695 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6397
SphI G_CATG'C
Cuts at: 1132 3755 1132
Size: 2623 3774
SspI AAT'ATT
Cuts at: 5 52 4321 5
Size: 47 4269 2081
Fragments arranged by size:
4269 2081 47
StuI AGG'CCT
Cuts at: 1517 3528 1517
Size: 2011 4386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2862 2967 3836 513
Size: 552 939 858 105 869 3074
Fragments arranged by size:
3074 939 869 858 552 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2330 3521 4424 4441 4594 4779
Size: 1255 555 195 1191 903 17 153 185
Cuts at: 4779 5224 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1191 903 555 445 195 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2652 3654 4643
Size: 274 80 33 51 1443 675 1002 989
Cuts at: 4643 6355 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1002 989 675 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2570 2602 6357 6389 2570
Size: 32 3755 32 2578
Fragments arranged by size:
3755 2578 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3194 1316
Size: 1878 4519
XbaI T'CTAG_A
Cuts at: 3733 3733
Size: 6397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3322 3322
Size: 6397
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6397
XmaI C'CCGG_G
Cuts at: 1870 3722 1870
Size: 1852 4545
XmnI GAAnn'nnTTC
Cuts at: 4526 4526
Size: 6397
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4205 276
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SanDI SfiI SrfI SwaI