Restriction map for
pFN21AE2268
(Circular) (Six-base) MAPSORT of: pFN21AE2268.seq Check: 7661 from: 1 to: 5089
pFN21AE2268 5089 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2899 278
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2432 1937
Size: 495 4594
Acc65I G'GTAC_C
Cuts at: 2410 2410
Size: 5089
AclI AA'CG_TT
Cuts at: 1563 3216 3589 1563
Size: 1653 373 3063
Fragments arranged by size:
3063 1653 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 4536 4804 1632
Size: 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4892
AflIII A'CryG_T
Cuts at: 1203 3988 4965 1203
Size: 2785 977 1327
Fragments arranged by size:
2785 1327 977
AgeI A'CCGG_T
Cuts at: 3920 3920
Size: 5089
AhdI GACnn_n'nnGTC
Cuts at: 3818 3818
Size: 5089
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 1184
Size: 1017 4072
AlwNI CAG_nnn'CTG
Cuts at: 2313 2832 4404 2313
Size: 519 1572 2998
Fragments arranged by size:
2998 1572 519
ApaLI G'TGCA_C
Cuts at: 2186 3146 4302 2186
Size: 960 1156 2973
Fragments arranged by size:
2973 1156 960
ApoI r'AATT_y
Cuts at: 1493 2398 2624 2776 4885 1493
Size: 905 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3643 160
Size: 872 2611 1606
Fragments arranged by size:
2611 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2279 2414 1870
Size: 73 271 65 135 4545
Fragments arranged by size:
4545 271 135 73 65
BamHI G'GATC_C
Cuts at: 2055 2419 2786 2055
Size: 364 367 4358
Fragments arranged by size:
4358 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2410 3865 618
Size: 325 217 627 248 375 1455 1842
Fragments arranged by size:
1842 1455 627 375 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2408 1391
Size: 1017 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3280 3314 1903
Size: 34 1343 34 3678
Fragments arranged by size:
3678 1343 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2981 4191 2981
Size: 1210 3879
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3700 136
Size: 107 122 71 631 605 2028 1525
Fragments arranged by size:
2028 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5084 5084
Size: 5089
BlpI GC'TnA_GC
Cuts at: 2506 2506
Size: 5089
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2190 3150 4306 1790
Size: 250 150 960 1156 2573
Fragments arranged by size:
2573 1156 960 250 150
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 3778 442
Size: 416 53 83 399 24 1403 958 1753
Fragments arranged by size:
1753 1403 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5089
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3749 1239
Size: 2510 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3214 4079 4377 3214
Size: 865 298 3926
Fragments arranged by size:
3926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3752 882
Size: 617 2253 2219
Fragments arranged by size:
2253 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2880 493
Size: 711 1676 2702
Fragments arranged by size:
2702 1676 711
BsaBI GATnn'nnATC
Cuts at: 5083 5083
Size: 5089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2896 3278 3913 275
Size: 53 83 186 936 1363 382 635 1451
Fragments arranged by size:
1451 1363 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3522 3920 4194 4341 4752 3522
Size: 398 274 147 411 3859
Fragments arranged by size:
3859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4864
Fragments arranged by size:
4864 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2218 1186
Size: 913 119 4057
Fragments arranged by size:
4057 913 119
BseYI C'CCAG_C
Cuts at: 2174 2250 4292 4788 2174
Size: 76 2042 496 2475
Fragments arranged by size:
2475 2042 496 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2539 3300 4328 1540
Size: 275 186 538 761 1028 2301
Fragments arranged by size:
2301 1028 761 538 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 3150 3235 4306 2190
Size: 960 85 1071 2973
Fragments arranged by size:
2973 1071 960 85
BsmI GAATG_Cn'
Cuts at: 2600 2693 2600
Size: 93 4996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2190 2408 3150 3235 4306 1391
Size: 399 250 150 218 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 250 218 150 85
BspEI T'CCGG_A
Cuts at: 4752 4752
Size: 5089
BspHI T'CATG_A
Cuts at: 2978 4708 2978
Size: 1730 3359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 844
Size: 1600 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2976 1394
Size: 429 1153 3507
Fragments arranged by size:
3507 1153 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3584 3758 65
Size: 1183 2336 174 1396
Fragments arranged by size:
2336 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3733 3920 1700
Size: 110 1923 187 2869
Fragments arranged by size:
2869 1923 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5089
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5089
BssSI C'ACGA_G
Cuts at: 3149 4161 3149
Size: 1012 4077
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5089
BstBI TT'CG_AA
Cuts at: 3893 3893
Size: 5089
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5089
BstXI CCAn_nnnn'nTGG
Cuts at: 2066 2066
Size: 5089
BstYI r'GATC_y
Cuts at: 1972 2055 2419 2786 3171 3188 4640 4726 4738
Size: 83 364 367 385 17 1452 86 12
Cuts at: 4738 5084 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 364 346 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2329 513
Size: 552 939 325 3273
Fragments arranged by size:
3273 939 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4350
Fragments arranged by size:
4350 683 56
BtsI GCAGTG_nn'
Cuts at: 2211 2605 3399 3419 2211
Size: 394 794 20 3881
Fragments arranged by size:
3881 794 394 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5089
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5054
DraI TTT'AAA
Cuts at: 2393 2393
Size: 5089
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4096 809
Size: 507 2780 1802
Fragments arranged by size:
2780 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2233 2332
Size: 54 1425 50 275 18 214 189 99
Cuts at: 2332 2536 3425 5054 8
Size: 204 889 1629 43
Fragments arranged by size:
1629 1425 889 275 214 204 189 99
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2536 1537
Size: 275 724 4090
Fragments arranged by size:
4090 724 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3019 1353
Size: 1666 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3676 4050 4196 387
Size: 1064 138 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3152 3749 4536 4804 1239
Size: 393 1520 597 787 268 1524
Fragments arranged by size:
1524 1520 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2131 2131
Size: 5089
EcoO109I rG'GnC_Cy
Cuts at: 2148 2148
Size: 5089
EcoRI G'AATT_C
Cuts at: 2398 2776 2398
Size: 378 4711
FspI TGC'GCA
Cuts at: 3595 3595
Size: 5089
HaeII r_GCGC'y
Cuts at: 1678 4236 1678
Size: 2558 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3730 3762 3804 3836 1373
Size: 32 135 32 2158 32 42 32 2626
Fragments arranged by size:
2626 2158 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2433 2679 669
Size: 988 281 495 246 3079
Fragments arranged by size:
3079 988 495 281 246
HindIII A'AGCT_T
Cuts at: 748 2016 748
Size: 1268 3821
HpaI GTT'AAC
Cuts at: 2679 2679
Size: 5089
KpnI G_GTAC'C
Cuts at: 2414 2414
Size: 5089
MfeI C'AATT_G
Cuts at: 2688 2688
Size: 5089
MluI A'CGCG_T
Cuts at: 4965 4965
Size: 5089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4203 4387 1305
Size: 2898 184 2007
Fragments arranged by size:
2898 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2334 10
Size: 54 1425 343 502 2765
Fragments arranged by size:
2765 1425 502 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3047 3406 3565 518
Size: 648 1017 864 359 159 2042
Fragments arranged by size:
2042 1017 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2300 2356 2505 3182 4330 4575 1606
Size: 648 46 56 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 648 245 149 56 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 2329 513
Size: 552 939 325 3273
Fragments arranged by size:
3273 939 552 325
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5089
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5089
NotI GC'GGCC_GC
Cuts at: 2536 2536
Size: 5089
NspI r_CATG'y
Cuts at: 1132 1229 2447 3992 1132
Size: 97 1218 1545 2229
Fragments arranged by size:
2229 1545 1218 97
PasI CC'CwG_GG
Cuts at: 1348 2155 1348
Size: 807 4282
PciI A'CATG_T
Cuts at: 3988 3988
Size: 5089
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2313 1108
Size: 1205 3884
PmeI GTTT'AAAC
Cuts at: 2393 2393
Size: 5089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 4671 4703 3158
Size: 32 1481 32 3544
Fragments arranged by size:
3544 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2108 1613
Size: 495 4594
PsiI TTA'TAA
Cuts at: 2659 2659
Size: 5089
PstI C_TGCA'G
Cuts at: 830 2441 830
Size: 1611 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5089
PvuII CAG'CTG
Cuts at: 2254 2356 2254
Size: 102 4987
SalI G'TCGA_C
Cuts at: 1936 2431 1936
Size: 495 4594
SbfI CC_TGCA'GG
Cuts at: 2441 2441
Size: 5089
ScaI AGT'ACT
Cuts at: 3337 5049 3337
Size: 1712 3377
SfcI C'TryA_G
Cuts at: 826 1045 2437 3572 4253 4444 826
Size: 219 1392 1135 681 191 1471
Fragments arranged by size:
1471 1392 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5089
SmaI CCC'GGG
Cuts at: 1872 2216 2281 2416 1872
Size: 344 65 135 4545
Fragments arranged by size:
4545 344 135 65
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3193 4094 4356 820
Size: 197 926 1250 901 262 1553
Fragments arranged by size:
1553 1250 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2880 493
Size: 2387 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5089
SphI G_CATG'C
Cuts at: 1132 2447 1132
Size: 1315 3774
SspI AAT'ATT
Cuts at: 5 52 3013 5
Size: 47 2961 2081
Fragments arranged by size:
2961 2081 47
StuI AGG'CCT
Cuts at: 1517 2065 1517
Size: 548 4541
StyI C'CwwG_G
Cuts at: 513 1065 2004 2329 2528 513
Size: 552 939 325 199 3074
Fragments arranged by size:
3074 939 552 325 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 3116 3133 3286 3471 3916 325
Size: 1255 509 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1027 509 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3335 5047 96
Size: 274 80 33 51 1443 1358 1712 138
Fragments arranged by size:
1712 1443 1358 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5049 5081 5049
Size: 32 5057
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5089
XbaI T'CTAG_A
Cuts at: 2425 2425
Size: 5089
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5089
XmaI C'CCGG_G
Cuts at: 1870 2214 2279 2414 1870
Size: 344 65 135 4545
Fragments arranged by size:
4545 344 135 65
XmnI GAAnn'nnTTC
Cuts at: 3218 3218
Size: 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2897 276
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI