Restriction map for
pFN21AE2267
(Circular) (Six-base) MAPSORT of: pFN21AE2267.seq Check: 5441 from: 1 to: 7549
pFN21AE2267 7549 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5359 278
Size: 53 83 186 936 3823 2468
Fragments arranged by size:
3823 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3714 4892 1937
Size: 1777 1178 4594
Fragments arranged by size:
4594 1777 1178
Acc65I G'GTAC_C
Cuts at: 4870 4870
Size: 7549
AclI AA'CG_TT
Cuts at: 1563 5676 6049 1563
Size: 4113 373 3063
Fragments arranged by size:
4113 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2534 3500 4011 5612 6996 7264 1632
Size: 902 966 511 1601 1384 268 1917
Fragments arranged by size:
1917 1601 1384 966 902 511 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7352
AflIII A'CryG_T
Cuts at: 1203 2731 2733 4221 4766 6448 7425 1203
Size: 1528 2 1488 545 1682 977 1327
Fragments arranged by size:
1682 1528 1488 1327 977 545 2
AgeI A'CCGG_T
Cuts at: 6380 6380
Size: 7549
AhdI GACnn_n'nnGTC
Cuts at: 6278 6278
Size: 7549
AleI CACnn'nnGTG
Cuts at: 1166 4755 1166
Size: 3589 3960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7549
AlwNI CAG_nnn'CTG
Cuts at: 3998 4760 5292 6864 3998
Size: 762 532 1572 4683
Fragments arranged by size:
4683 1572 762 532
ApaI G_GGCC'C
Cuts at: 3193 3676 3193
Size: 483 7066
ApaLI G'TGCA_C
Cuts at: 5606 6762 5606
Size: 1156 6393
ApoI r'AATT_y
Cuts at: 1493 3440 3773 4666 4858 5084 5236 7345 1493
Size: 1947 333 893 192 226 152 2109 1697
Fragments arranged by size:
2109 1947 1697 893 333 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 6103 160
Size: 872 5071 1606
Fragments arranged by size:
5071 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2516 3280 3554 4874 1870
Size: 73 194 379 764 274 1320 4545
Fragments arranged by size:
4545 1320 764 379 274 194 73
BamHI G'GATC_C
Cuts at: 2120 3733 4879 5246 2120
Size: 1613 1146 367 4423
Fragments arranged by size:
4423 1613 1146 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4340 4439 4870 6325 618
Size: 325 217 627 2553 99 431 1455 1842
Fragments arranged by size:
2553 1842 1455 627 431 325 217 99
BanII G_rGCy'C
Cuts at: 1391 2138 3193 3676 4641 4868 1391
Size: 747 1055 483 965 227 4072
Fragments arranged by size:
4072 1055 965 747 483 227
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3912 4216 4821 928
Size: 971 2013 304 605 3656
Fragments arranged by size:
3656 2013 971 605 304
BbvCI CC'TCA_GC
Cuts at: 4559 4559
Size: 7549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5740 5774 1903
Size: 34 3803 34 3678
Fragments arranged by size:
3803 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5441 6651 5441
Size: 1210 6339
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7549
BfrBI ATG'CAT
Cuts at: 3584 3584
Size: 7549
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6160 136
Size: 107 122 71 631 605 4488 1525
Fragments arranged by size:
4488 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4254 7544 4254
Size: 3290 4259
BlpI GC'TnA_GC
Cuts at: 4966 4966
Size: 7549
Bme1580I G_kGCm'C
Cuts at: 1790 3193 3676 5610 6766 1790
Size: 1403 483 1934 1156 2573
Fragments arranged by size:
2573 1934 1403 1156 483
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7549
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3085 5280 6238
Size: 416 53 83 399 24 1668 2195 958
Cuts at: 6238 442
Size: 1753
Fragments arranged by size:
2195 1753 1668 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7549
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2954 2954
Size: 7549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3230 3551 3841 6209 1239
Size: 1991 321 290 2368 2579
Fragments arranged by size:
2579 2368 1991 321 290
Bpu10I CC'TnA_GC
Cuts at: 4559 4559
Size: 7549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2907 5674 6539 6837 2907
Size: 2767 865 298 3619
Fragments arranged by size:
3619 2767 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4015 6212 882
Size: 617 2516 2197 2219
Fragments arranged by size:
2516 2219 2197 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 5340 493
Size: 711 1530 2606 2702
Fragments arranged by size:
2702 2606 1530 711
BsaBI GATnn'nnATC
Cuts at: 7543 7543
Size: 7549
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2708 4213 5356 5738
Size: 53 83 186 936 1175 1505 1143 382
Cuts at: 5738 6373 275
Size: 635 1451
Fragments arranged by size:
1505 1451 1175 1143 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5982 6380 6654 6801 7212 5982
Size: 398 274 147 411 6319
Fragments arranged by size:
6319 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3071 3101 1178
Size: 30 165 30 1668 30 5626
Fragments arranged by size:
5626 1668 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 4609 1186
Size: 1012 2411 4126
Fragments arranged by size:
4126 2411 1012
BseYI C'CCAG_C
Cuts at: 2310 6752 7248 2310
Size: 4442 496 2611
Fragments arranged by size:
4442 2611 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 2663 4619 1163
Size: 573 347 580 1956 4093
Fragments arranged by size:
4093 1956 580 573 347
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 3070 4999 5760 6788 1540
Size: 275 186 77 992 1929 761 1028 2301
Fragments arranged by size:
2301 1929 1028 992 761 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 2970 2985 3972 5610 5695 6766 2970
Size: 15 987 1638 85 1071 3753
Fragments arranged by size:
3753 1638 1071 987 85 15
BsmI GAATG_Cn'
Cuts at: 2693 4614 5060 5153 2693
Size: 1921 446 93 5089
Fragments arranged by size:
5089 1921 446 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2534 2534
Size: 7549
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2970 2985 3193 3676 3972 4641
Size: 399 348 832 15 208 483 296 669
Cuts at: 4641 4868 5610 5695 6766 1391
Size: 227 742 85 1071 2174
Fragments arranged by size:
2174 1071 832 742 669 483 399 348
296 227 208 85 15
BspEI T'CCGG_A
Cuts at: 7212 7212
Size: 7549
BspHI T'CATG_A
Cuts at: 5438 7168 5438
Size: 1730 5819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 4904 844
Size: 1211 2849 3489
Fragments arranged by size:
3489 2849 1211
BsrBI CCG'CTC
Cuts at: 1394 1823 5436 1394
Size: 429 3613 3507
Fragments arranged by size:
3613 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 4024 6044 6218 65
Size: 1183 1701 1075 2020 174 1396
Fragments arranged by size:
2020 1701 1396 1183 1075 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6193 6380 1700
Size: 110 4383 187 2869
Fragments arranged by size:
4383 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7549
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7549
BssSI C'ACGA_G
Cuts at: 3617 3965 5609 6621 3617
Size: 348 1644 1012 4545
Fragments arranged by size:
4545 1644 1012 348
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7549
BstBI TT'CG_AA
Cuts at: 6353 6353
Size: 7549
BstEII G'GTnAC_C
Cuts at: 1184 2585 1184
Size: 1401 6148
BstYI r'GATC_y
Cuts at: 1972 2120 3169 3733 4022 4254 4879 5246 5631
Size: 148 1049 564 289 232 625 367 385
Cuts at: 5631 5648 7100 7186 7198 7544 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1049 625 564 385 367 346
289 232 148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2029 2078 3107 513
Size: 552 964 49 1029 4955
Fragments arranged by size:
4955 1029 964 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6810
Fragments arranged by size:
6810 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 2985 3014 4527 5065 5859 5879 2066
Size: 919 29 1513 538 794 20 3736
Fragments arranged by size:
3736 1513 919 794 538 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7549
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7514
DraI TTT'AAA
Cuts at: 3424 3838 4410 4853 3424
Size: 414 572 443 6120
Fragments arranged by size:
6120 572 443 414
DraIII CAC_nnn'GTG
Cuts at: 2974 2974
Size: 7549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6556 809
Size: 507 5240 1802
Fragments arranged by size:
5240 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 3063 4368
Size: 54 1425 50 275 18 245 988 1305
Cuts at: 4368 4479 4996 5885 7514 8
Size: 111 517 889 1629 43
Fragments arranged by size:
1629 1425 1305 988 889 517 275 245
111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 4996 1537
Size: 275 263 2921 4090
Fragments arranged by size:
4090 2921 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 3280 3566 3888 5479 1353
Size: 1927 286 322 1591 3423
Fragments arranged by size:
3423 1927 1591 322 286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2807 2993 6136 6510 6656 387
Size: 1064 138 1218 186 3143 374 146 1280
Fragments arranged by size:
3143 1280 1218 1064 374 186 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 3230 3500 3551 3841 4011 5612
Size: 393 902 696 270 51 290 170 1601
Cuts at: 5612 6209 6996 7264 1239
Size: 597 787 268 1524
Fragments arranged by size:
1601 1524 902 787 696 597 393 290
270 268 170 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3197 3197
Size: 7549
EcoO109I rG'GnC_Cy
Cuts at: 2067 3189 3190 3672 4468 4711 2067
Size: 1122 1 482 796 243 4905
Fragments arranged by size:
4905 1122 796 482 243 1
EcoRI G'AATT_C
Cuts at: 3440 4858 5236 3440
Size: 1418 378 5753
Fragments arranged by size:
5753 1418 378
FspI TGC'GCA
Cuts at: 6055 6055
Size: 7549
HaeII r_GCGC'y
Cuts at: 1678 6696 1678
Size: 5018 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2291 2323 3796 3828 4713
Size: 32 135 32 719 32 1473 32 885
Cuts at: 4713 4745 6190 6222 6264 6296 1373
Size: 32 1445 32 42 32 2626
Fragments arranged by size:
2626 1473 1445 885 719 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3520 4893 5139 669
Size: 988 281 1582 1373 246 3079
Fragments arranged by size:
3079 1582 1373 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7549
HpaI GTT'AAC
Cuts at: 5139 5139
Size: 7549
KpnI G_GTAC'C
Cuts at: 4874 4874
Size: 7549
MfeI C'AATT_G
Cuts at: 5148 5148
Size: 7549
MluI A'CGCG_T
Cuts at: 7425 7425
Size: 7549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2919 3054 3465 6663 6847 1305
Size: 1614 135 411 3198 184 2007
Fragments arranged by size:
3198 2007 1614 411 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5727
Fragments arranged by size:
5727 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4226 4239 4487 4595 4755 4771 5507
Size: 648 3060 13 248 108 160 16 736
Cuts at: 5507 5866 6025 518
Size: 359 159 2042
Fragments arranged by size:
3060 2042 736 648 359 248 160 159
108 16 13
MspA1I CmG'CkG
Cuts at: 1606 2031 2080 3075 4965 5642 6790 7035 1606
Size: 425 49 995 1890 677 1148 245 2120
Fragments arranged by size:
2120 1890 1148 995 677 425 245 49
NcoI C'CATG_G
Cuts at: 513 1065 3107 513
Size: 552 2042 4955
Fragments arranged by size:
4955 2042 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7549
NotI GC'GGCC_GC
Cuts at: 2075 4996 2075
Size: 2921 4628
NsiI A_TGCA'T
Cuts at: 3586 3586
Size: 7549
NspI r_CATG'y
Cuts at: 1132 1229 2046 3004 4770 4907 6452 1132
Size: 97 817 958 1766 137 1545 2229
Fragments arranged by size:
2229 1766 1545 958 817 137 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7549
PciI A'CATG_T
Cuts at: 4766 6448 4766
Size: 1682 5867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7549
PfoI T'CCnGG_A
Cuts at: 2725 3154 3165 3729 2725
Size: 429 11 564 6545
Fragments arranged by size:
6545 564 429 11
PmeI GTTT'AAAC
Cuts at: 4853 4853
Size: 7549
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 7549
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5618 5650 7131 7163 5618
Size: 32 1481 32 6004
Fragments arranged by size:
6004 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 4468 4711 2067
Size: 2401 243 4905
Fragments arranged by size:
4905 2401 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7549
PsiI TTA'TAA
Cuts at: 3864 5119 3864
Size: 1255 6294
PspOMI G'GGCC_C
Cuts at: 3189 3672 3189
Size: 483 7066
PstI C_TGCA'G
Cuts at: 830 3009 3339 3960 4330 4901 830
Size: 2179 330 621 370 571 3478
Fragments arranged by size:
3478 2179 621 571 370 330
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7549
PvuII CAG'CTG
Cuts at: 3075 3075
Size: 7549
SacII CC_GC'GG
Cuts at: 2032 2081 2032
Size: 49 7500
SalI G'TCGA_C
Cuts at: 1936 4891 1936
Size: 2955 4594
SapI GCTCTTCn'nnn_
Cuts at: 3566 3566
Size: 7549
SbfI CC_TGCA'GG
Cuts at: 4901 4901
Size: 7549
ScaI AGT'ACT
Cuts at: 2189 5797 7509 2189
Size: 3608 1712 2229
Fragments arranged by size:
3608 2229 1712
SfcI C'TryA_G
Cuts at: 826 1045 3005 3335 3956 4299 4326 4897 6032
Size: 219 1960 330 621 343 27 571 1135
Cuts at: 6032 6713 6904 826
Size: 681 191 1471
Fragments arranged by size:
1960 1471 1135 681 621 571 343 330
219 191 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7549
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7549
SmaI CCC'GGG
Cuts at: 1872 2139 2518 3282 4876 1872
Size: 267 379 764 1594 4545
Fragments arranged by size:
4545 1594 764 379 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2922 5653 6554 6816 820
Size: 197 926 979 2731 901 262 1553
Fragments arranged by size:
2731 1553 979 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5340 493
Size: 4847 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7549
SphI G_CATG'C
Cuts at: 1132 2046 4907 1132
Size: 914 2861 3774
Fragments arranged by size:
3774 2861 914
SrfI GCCC'GGGC
Cuts at: 2139 2139
Size: 7549
SspI AAT'ATT
Cuts at: 5 52 3636 4462 5473 5
Size: 47 3584 826 1011 2081
Fragments arranged by size:
3584 2081 1011 826 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7549
StyI C'CwwG_G
Cuts at: 513 1065 3107 3539 4717 4988 513
Size: 552 2042 432 1178 271 3074
Fragments arranged by size:
3074 2042 1178 552 432 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 3929 5576 5593 5746 5931 6376
Size: 1255 993 1356 1647 17 153 185 445
Cuts at: 6376 325
Size: 1498
Fragments arranged by size:
1647 1498 1356 1255 993 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 3289 5795
Size: 274 80 33 51 1443 210 1102 2506
Cuts at: 5795 7507 96
Size: 1712 138
Fragments arranged by size:
2506 1712 1443 1102 274 210 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 3260 3292 4507 4539 7509 7541 2549
Size: 32 679 32 1215 32 2970 32 2557
Fragments arranged by size:
2970 2557 1215 679 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7549
XbaI T'CTAG_A
Cuts at: 4885 4885
Size: 7549
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7549
XmaI C'CCGG_G
Cuts at: 1870 2137 2516 3280 4874 1870
Size: 267 379 764 1594 4545
Fragments arranged by size:
4545 1594 764 379 267
XmnI GAAnn'nnTTC
Cuts at: 3612 5678 3612
Size: 2066 5483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5357 276
Size: 53 83 186 936 3823 2468
Fragments arranged by size:
3823 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI
RsrII SacI SanDI SexAI SfiI SfoI SwaI XcmI