Restriction map for
pFN21AE2265
(Circular) (Six-base) MAPSORT of: pFN21AE2265.seq Check: 7641 from: 1 to: 6049
pFN21AE2265 6049 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2320 3220 2223
Size: 97 900 5052
Fragments arranged by size:
5052 900 97
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3392 1937
Size: 1455 4594
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2915 2967 3029 3209 3324 4112 5496 5764
Size: 1283 52 62 180 115 788 1384 268
Cuts at: 5764 1632
Size: 1917
Fragments arranged by size:
1917 1384 1283 788 268 180 115 62
52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 4948 5925 1203
Size: 3745 977 1327
Fragments arranged by size:
3745 1327 977
AgeI A'CCGG_T
Cuts at: 2182 4880 2182
Size: 2698 3351
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2878 1184
Size: 1694 4355
AlwNI CAG_nnn'CTG
Cuts at: 2215 2224 2253 3197 3792 5364 2215
Size: 9 29 944 595 1572 2900
Fragments arranged by size:
2900 1572 944 595 29 9
ApaI G_GGCC'C
Cuts at: 2299 2299
Size: 6049
ApaLI G'TGCA_C
Cuts at: 4106 5262 4106
Size: 1156 4893
ApoI r'AATT_y
Cuts at: 1493 3258 3358 3584 3736 5845 1493
Size: 1765 100 226 152 2109 1697
Fragments arranged by size:
2109 1765 1697 226 152 100
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 3374 1870
Size: 73 1431 4545
Fragments arranged by size:
4545 1431 73
BamHI G'GATC_C
Cuts at: 2275 3379 3746 2275
Size: 1104 367 4578
Fragments arranged by size:
4578 1104 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2862 3370 4825 618
Size: 325 217 627 1075 508 1455 1842
Fragments arranged by size:
1842 1455 1075 627 508 325 217
BanII G_rGCy'C
Cuts at: 1391 2299 3368 1391
Size: 908 1069 4072
Fragments arranged by size:
4072 1069 908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 3257 928
Size: 971 546 812 3720
Fragments arranged by size:
3720 971 812 546
BbvCI CC'TCA_GC
Cuts at: 2225 3093 2225
Size: 868 5181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4240 4274 1903
Size: 34 2303 34 3678
Fragments arranged by size:
3678 2303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2630 3099 3941 5151 2630
Size: 469 842 1210 3528
Fragments arranged by size:
3528 1210 842 469
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3178 4660 136
Size: 107 122 71 631 605 1506 1482 1525
Fragments arranged by size:
1525 1506 1482 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2299 4110 5266 1790
Size: 509 1811 1156 2573
Fragments arranged by size:
2573 1811 1156 509
BmgBI CAC'GTC
Cuts at: 1318 2638 1318
Size: 1320 4729
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2638 3780 4738
Size: 416 53 83 399 24 1221 1142 958
Cuts at: 4738 442
Size: 1753
Fragments arranged by size:
1753 1221 1142 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2129 2129
Size: 6049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2547 2727 3012 4709 1239
Size: 860 448 180 285 1697 2579
Fragments arranged by size:
2579 1697 860 448 285 180
Bpu10I CC'TnA_GC
Cuts at: 2225 3093 2225
Size: 868 5181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 2858 4174 5039 5337 2351
Size: 507 1316 865 298 3063
Fragments arranged by size:
3063 1316 865 507 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 4712 882
Size: 617 984 2229 2219
Fragments arranged by size:
2229 2219 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2511 3856 4238 4873
Size: 53 83 186 936 978 1345 382 635
Cuts at: 4873 275
Size: 1451
Fragments arranged by size:
1451 1345 978 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2182 2476 2545 4482 4880 5154 5301 5712 2182
Size: 294 69 1937 398 274 147 411 2519
Fragments arranged by size:
2519 1937 411 398 294 274 147 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2545 2575 1178
Size: 30 165 30 1142 30 4652
Fragments arranged by size:
4652 1142 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6049
BseYI C'CCAG_C
Cuts at: 2094 2188 2343 2581 2595 2939 5252 5748 2094
Size: 94 155 238 14 344 2313 496 2395
Fragments arranged by size:
2395 2313 496 344 238 155 94 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3248 1163
Size: 573 1512 3964
Fragments arranged by size:
3964 1512 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4110 4195 5266 4110
Size: 85 1071 4893
Fragments arranged by size:
4893 1071 85
BsmI GAATG_Cn'
Cuts at: 3329 3560 3653 3329
Size: 231 93 5725
Fragments arranged by size:
5725 231 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2189 2189
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 3368 4110 4195 5266 1391
Size: 399 509 1069 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 742 509 399 85
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 2320 3220 3404 844
Size: 1379 97 900 184 3489
Fragments arranged by size:
3489 1379 900 184 97
BsrBI CCG'CTC
Cuts at: 1394 1823 3936 1394
Size: 429 2113 3507
Fragments arranged by size:
3507 2113 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2750 2961 4544 4718 65
Size: 1183 1502 211 1583 174 1396
Fragments arranged by size:
1583 1502 1396 1183 211 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 4693 4880 1700
Size: 110 372 2511 187 2869
Fragments arranged by size:
2869 2511 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 3197 794
Size: 1421 982 3646
Fragments arranged by size:
3646 1421 982
BstBI TT'CG_AA
Cuts at: 2288 4853 2288
Size: 2565 3484
BstEII G'GTnAC_C
Cuts at: 1184 2585 1184
Size: 1401 4648
BstYI r'GATC_y
Cuts at: 1972 2275 2722 3316 3379 3746 4131 4148 5600
Size: 303 447 594 63 367 385 17 1452
Cuts at: 5600 5686 5698 6044 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 594 447 385 367 346 303
86 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2055 2590 3245 513
Size: 552 990 535 655 3317
Fragments arranged by size:
3317 990 655 552 535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5310
Fragments arranged by size:
5310 683 56
BtsI GCAGTG_nn'
Cuts at: 2465 2573 2574 3565 4359 4379 2465
Size: 108 1 991 794 20 4135
Fragments arranged by size:
4135 991 794 108 20 1
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2149 2149
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3496 4385 6014
Size: 54 1425 50 275 18 1666 889 1629
Cuts at: 6014 8
Size: 43
Fragments arranged by size:
1666 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 3225 3979 1353
Size: 932 940 754 3423
Fragments arranged by size:
3423 940 932 754
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2547 2727 2915 2967 3012 3029
Size: 393 467 448 180 188 52 45 17
Cuts at: 3029 3209 3324 4112 4709 5496 5764 1239
Size: 180 115 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 597 467 448 393 268
188 180 180 115 52 45 17
EcoO109I rG'GnC_Cy
Cuts at: 2295 2295
Size: 6049
EcoRI G'AATT_C
Cuts at: 3358 3736 3358
Size: 378 5671
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 2180 5196 1678
Size: 502 3016 2531
Fragments arranged by size:
3016 2531 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2466 2498 4690
Size: 32 135 32 496 32 366 32 2192
Cuts at: 4690 4722 4764 4796 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2192 496 366 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3393 3639 669
Size: 988 281 1455 246 3079
Fragments arranged by size:
3079 1455 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 748
Size: 1930 4119
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2361 3295 5163 5347 1305
Size: 1056 934 1868 184 2007
Fragments arranged by size:
2007 1868 1056 934 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4227
Fragments arranged by size:
4227 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2772 2793 3015 4007 4366 4525 518
Size: 648 1606 21 222 992 359 159 2042
Fragments arranged by size:
2042 1606 992 648 359 222 159 21
MspA1I CmG'CkG
Cuts at: 1606 3465 4142 5290 5535 1606
Size: 1859 677 1148 245 2120
Fragments arranged by size:
2120 1859 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2590 3245 513
Size: 552 1525 655 3317
Fragments arranged by size:
3317 1525 655 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2609 3407 4952 1132
Size: 97 1380 798 1545 2229
Fragments arranged by size:
2229 1545 1380 798 97
PasI CC'CwG_GG
Cuts at: 1348 2299 3032 1348
Size: 951 733 4365
Fragments arranged by size:
4365 951 733
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2460 2521 3019 3114 1108
Size: 1352 61 498 95 4043
Fragments arranged by size:
4043 1352 498 95 61
PfoI T'CCnGG_A
Cuts at: 2271 3312 2271
Size: 1041 5008
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 2628 3619 2628
Size: 991 5058
PspOMI G'GGCC_C
Cuts at: 2295 2295
Size: 6049
PstI C_TGCA'G
Cuts at: 830 2220 3202 3401 830
Size: 1390 982 199 3478
Fragments arranged by size:
3478 1390 982 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 2220 3401 2220
Size: 1181 4868
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 2216 2814 3198 3397 4532 5213 5404
Size: 219 1171 598 384 199 1135 681 191
Cuts at: 5404 826
Size: 1471
Fragments arranged by size:
1471 1171 1135 681 598 384 219 199
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 2837 4153 5054 5316 820
Size: 197 926 387 507 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 507 387 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 2908 3973 5
Size: 47 2856 1065 2081
Fragments arranged by size:
2856 2081 1065 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2499 2590 3245 3488 513
Size: 552 1434 91 655 243 3074
Fragments arranged by size:
3074 1434 655 552 243 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2335 2573 4076 4093 4246 4431 4876
Size: 1255 755 238 1503 17 153 185 445
Cuts at: 4876 325
Size: 1498
Fragments arranged by size:
1503 1498 1255 755 445 238 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4295 6007 96
Size: 274 80 33 51 1443 2318 1712 138
Fragments arranged by size:
2318 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 6009 6041 2496
Size: 32 3481 32 2504
Fragments arranged by size:
3481 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6049
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2873 3119 2873
Size: 246 5803
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 4178 4178
Size: 6049
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI