Restriction map for
pFN21AE2264
(Circular) (Six-base) MAPSORT of: pFN21AE2264.seq Check: 3691 from: 1 to: 5707
pFN21AE2264 5707 bp
With 182 enzymes: SgfI *
March 28, 2012 12:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2841 3050 1937
Size: 904 209 4594
Fragments arranged by size:
4594 904 209
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2663 2898 3011 3770 5154 5422 1632
Size: 815 216 235 113 759 1384 268 1917
Fragments arranged by size:
1917 1384 815 759 268 235 216 113
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 2271 4436 2271
Size: 2165 3542
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 3450 5022 3450
Size: 1572 4135
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2903 3016 3242 3394 5503 1493
Size: 1410 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2220 2871 3032 1870
Size: 73 110 167 651 161 4545
Fragments arranged by size:
4545 651 167 161 110 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 3028 4483 618
Size: 325 217 627 300 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 300 217
BanII G_rGCy'C
Cuts at: 1391 3026 1391
Size: 1635 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 928
Size: 971 371 4365
Fragments arranged by size:
4365 971 371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2377 2411 3898 3932 1903
Size: 34 440 34 1487 34 3678
Fragments arranged by size:
3678 1487 440 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2302 3599 4809 2302
Size: 1297 1210 3200
Fragments arranged by size:
3200 1297 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2338 2515 5702 2338
Size: 177 3187 2343
Fragments arranged by size:
3187 2343 177
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2534 2534
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2341 2604 2948 4367 1239
Size: 1102 263 344 1419 2579
Fragments arranged by size:
2579 1419 1102 344 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2517 2765 4370 882
Size: 617 1018 248 1605 2219
Fragments arranged by size:
2219 1605 1018 617 248
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 2337 5701 2337
Size: 3364 2343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2746 3514 3896 4531
Size: 53 83 186 936 1213 768 382 635
Cuts at: 4531 275
Size: 1451
Fragments arranged by size:
1451 1213 936 768 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2958 4910 5406 2958
Size: 1952 496 3259
Fragments arranged by size:
3259 1952 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 4489
Fragments arranged by size:
4489 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2838 3157 3918 4946 1540
Size: 275 186 837 319 761 1028 2301
Fragments arranged by size:
2301 1028 837 761 319 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 2446 3218 3311 2446
Size: 772 93 4842
Fragments arranged by size:
4842 772 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2737 2737
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3026 3768 3853 4924 1391
Size: 399 1236 742 85 1071 2174
Fragments arranged by size:
2174 1236 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 2724 3596 5326 2724
Size: 872 1730 3105
Fragments arranged by size:
3105 1730 872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2745 2783 3062 844
Size: 1255 646 38 279 3489
Fragments arranged by size:
3489 1255 646 279 38
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 2856 96
Size: 2760 2947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 2386 3767 4779 2386
Size: 1381 1012 3314
Fragments arranged by size:
3314 1381 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2409 2938 794
Size: 1615 529 3563
Fragments arranged by size:
3563 1615 529
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 2865 1184
Size: 1681 4026
BstYI r'GATC_y
Cuts at: 1972 2338 2515 3037 3404 3789 3806 5258 5344
Size: 366 177 522 367 385 17 1452 86
Cuts at: 5344 5356 5702 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 522 385 367 366 346 177
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 3993
Fragments arranged by size:
3993 939 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2167 2167
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2779 4714 809
Size: 507 1463 1935 1802
Fragments arranged by size:
1935 1802 1463 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2835 3154
Size: 54 1425 50 275 18 177 828 319
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 828 319 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2835 3154 1537
Size: 275 1023 319 4090
Fragments arranged by size:
4090 1023 319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 3637 1353
Size: 1182 1102 3423
Fragments arranged by size:
3423 1182 1102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2341 2447 2604 2663 2898 2948 3011
Size: 393 709 106 157 59 235 50 63
Cuts at: 3011 3770 4367 5154 5422 1239
Size: 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 709 597 393 268 235
157 106 63 59 50
EcoRI G'AATT_C
Cuts at: 2903 3016 3394 2903
Size: 113 378 5216
Fragments arranged by size:
5216 378 113
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 4348 4380 4422
Size: 32 135 32 685 32 2059 32 42
Cuts at: 4422 4454 1373
Size: 32 2626
Fragments arranged by size:
2626 2059 685 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 2368 2842 3051 3297 669
Size: 988 281 352 78 474 209 246 3079
Fragments arranged by size:
3079 988 474 352 281 246 209 78
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 2573 3306 2573
Size: 733 4974
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3708
Fragments arranged by size:
3708 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3123 3800 4948 5193 1606
Size: 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 3993
Fragments arranged by size:
3993 939 552 223
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2319 1108
Size: 1211 4496
PfoI T'CCnGG_A
Cuts at: 2907 2907
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 2246 3277 2246
Size: 1031 4676
PspXI vC'TCGA_Gb
Cuts at: 2053 2220 2871 2053
Size: 167 651 4889
Fragments arranged by size:
4889 651 167
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 2840 3049 1936
Size: 904 209 4594
Fragments arranged by size:
4594 904 209
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2220 2871 3811 4712 4974
Size: 197 926 110 167 651 940 901 262
Cuts at: 4974 820
Size: 1553
Fragments arranged by size:
1553 940 926 901 651 262 197 167
110
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 2357 3631 5
Size: 47 2305 1274 2081
Fragments arranged by size:
2305 2081 1274 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2227 2293 3146 513
Size: 552 939 223 66 853 3074
Fragments arranged by size:
3074 939 853 552 223 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2693 2808 3734 3751 3904 4089 4534
Size: 1255 1113 115 926 17 153 185 445
Cuts at: 4534 325
Size: 1498
Fragments arranged by size:
1498 1255 1113 926 445 185 153 115
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 3953 5665
Size: 274 80 33 51 1443 879 1097 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1097 879 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2889 2921 5667 5699 2889
Size: 32 2746 32 2897
Fragments arranged by size:
2897 2746 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 2437 3043 2437
Size: 606 5101
XhoI C'TCGA_G
Cuts at: 1943 2053 2220 2871 1943
Size: 110 167 651 4779
Fragments arranged by size:
4779 651 167 110
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 3836 2340
Size: 1496 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI