Restriction map for
pFN21AE2263
(Circular) (Six-base) MAPSORT of: pFN21AE2263.seq Check: 2505 from: 1 to: 5860
pFN21AE2263 5860 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2731 3203 1937
Size: 794 472 4594
Fragments arranged by size:
4594 794 472
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 2382 3987 4360 1563
Size: 819 1605 373 3063
Fragments arranged by size:
3063 1605 819 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3923 5307 5575 1632
Size: 2291 1384 268 1917
Fragments arranged by size:
2291 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 3136 4759 5736 1203
Size: 1933 1623 977 1327
Fragments arranged by size:
1933 1623 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2682 2859 1184
Size: 1498 177 4185
Fragments arranged by size:
4185 1498 177
AlwNI CAG_nnn'CTG
Cuts at: 3032 3603 5175 3032
Size: 571 1572 3717
Fragments arranged by size:
3717 1572 571
ApaI G_GGCC'C
Cuts at: 2037 2683 2037
Size: 646 5214
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 2279 2760 3169 3395 3547 5656 1493
Size: 786 481 409 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 481 409 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2637 2893 3185 1870
Size: 73 694 256 292 4545
Fragments arranged by size:
4545 694 292 256 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2154 2187 2154
Size: 33 5827
BamHI G'GATC_C
Cuts at: 2817 3190 3557 2817
Size: 373 367 5120
Fragments arranged by size:
5120 373 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3181 4636 618
Size: 325 217 627 1394 1455 1842
Fragments arranged by size:
1842 1455 1394 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2683 2902 2960 3082 3179 1391
Size: 646 646 219 58 122 97 4072
Fragments arranged by size:
4072 646 646 219 122 97 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2988 928
Size: 971 1089 3800
Fragments arranged by size:
3800 1089 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4051 4085 1903
Size: 34 50 34 2030 34 3678
Fragments arranged by size:
3678 2030 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2182 3752 4962 2182
Size: 1570 1210 3080
Fragments arranged by size:
3080 1570 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2906 4471 136
Size: 107 122 71 631 605 1234 1565 1525
Fragments arranged by size:
1565 1525 1234 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2683 3921 5077 1790
Size: 247 646 1238 1156 2573
Fragments arranged by size:
2573 1238 1156 646 247
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2701 3591 4549
Size: 416 53 83 399 24 1284 890 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1284 958 890 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2376 2578 2597 2974 3094 4520 1239
Size: 1137 202 19 377 120 1426 2579
Fragments arranged by size:
2579 1426 1137 377 202 120 19
Bpu10I CC'TnA_GC
Cuts at: 3025 3051 3081 3025
Size: 26 30 5804
Fragments arranged by size:
5804 30 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3139 3651 493
Size: 711 1935 512 2702
Fragments arranged by size:
2702 1935 711 512
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3667 4049 4684 275
Size: 53 83 186 936 2134 382 635 1451
Fragments arranged by size:
2134 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 1186
Size: 1483 4377
BseYI C'CCAG_C
Cuts at: 2374 2675 2812 2974 5063 5559 2374
Size: 301 137 162 2089 496 2675
Fragments arranged by size:
2675 2089 496 301 162 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 1163
Size: 573 468 4819
Fragments arranged by size:
4819 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3921 4006 5077 3921
Size: 85 1071 4704
Fragments arranged by size:
4704 1071 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2683 2902 2960 3082 3179 3921
Size: 399 247 646 219 58 122 97 742
Cuts at: 3921 4006 5077 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 399 247 219 122
97 85 58
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 2094 3749 5479 2094
Size: 1655 1730 2475
Fragments arranged by size:
2475 1730 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 4355 4529 65
Size: 1183 1000 2107 174 1396
Fragments arranged by size:
2107 1396 1183 1000 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3095 4504 4691 1700
Size: 110 1285 1409 187 2869
Fragments arranged by size:
2869 1409 1285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 2022 2024 2026 1925
Size: 97 2 2 5759
Fragments arranged by size:
5759 97 2 2
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 2887 1184
Size: 1703 4157
BstYI r'GATC_y
Cuts at: 1972 2817 3190 3557 3942 3959 5411 5497 5509
Size: 845 373 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 845 385 373 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3045 3045
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2045 513
Size: 552 980 4328
Fragments arranged by size:
4328 980 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5121
Fragments arranged by size:
5121 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DraIII CAC_nnn'GTG
Cuts at: 2227 3142 2227
Size: 915 4945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2745 3097 3118
Size: 54 1425 50 275 18 915 352 21
Cuts at: 3118 3307 4196 5825 8
Size: 189 889 1629 43
Fragments arranged by size:
1629 1425 915 889 352 275 189 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2332 3790 1353
Size: 979 1458 3423
Fragments arranged by size:
3423 1458 979
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2376 2578 2597 2974 3094 3923 4520
Size: 393 744 202 19 377 120 829 597
Cuts at: 4520 5307 5575 1239
Size: 787 268 1524
Fragments arranged by size:
1524 829 787 744 597 393 377 268
202 120 19
EcoO109I rG'GnC_Cy
Cuts at: 2033 2629 2679 3029 3036 2033
Size: 596 50 350 7 4857
Fragments arranged by size:
4857 596 350 50 7
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 5007 1678
Size: 3329 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373
Size: 32 135 32 2929 32 42 32 2626
Fragments arranged by size:
2929 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2800 4974 5158 1305
Size: 1495 2174 184 2007
Fragments arranged by size:
2174 2007 1495 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3123
Fragments arranged by size:
3123 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 3818 4177 4336 518
Size: 648 1075 1577 359 159 2042
Fragments arranged by size:
2042 1577 1075 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2598 2952 3276 3953 5101 5346 1606
Size: 441 551 354 324 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 551 441 354 324 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2140 1348
Size: 792 5068
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PmlI CAC'GTG
Cuts at: 3139 3139
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3029 3036 3029
Size: 7 5853
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2033 2679 2033
Size: 646 5214
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2598 2952 2598
Size: 354 5506
SacII CC_GC'GG
Cuts at: 2048 2048
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 3019 3208 4343 5024 5215 826
Size: 219 1974 189 1135 681 191 1471
Fragments arranged by size:
1974 1471 1135 681 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2722 1517
Size: 1205 4655
StyI C'CwwG_G
Cuts at: 513 1065 2203 3299 513
Size: 552 1138 1096 3074
Fragments arranged by size:
3074 1138 1096 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 2149 2431 3989 2149
Size: 282 1558 4020
Fragments arranged by size:
4020 1558 282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI